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Sequence requirements for function of the
Sequence requirements for function of the

... re-introduced into the Drosophila genome via P elementmediated germline transformation, and these transgenic constructs can amplify with the correct tissue and temporal specificity (deCicco and Spradling, 1984). However, amplification is highly sensitive to chromosomal position effects and only ~1/3 ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007

... differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical differences of strands of DNA. Most importantly, these proposals challenge our understanding of, and ability to manipulate and probe, physical processes at the i ...
FULL TEXT - Journal of Pre-Clinical and Clinical Research
FULL TEXT - Journal of Pre-Clinical and Clinical Research

Stage-specific expressions of four different ribonuclease H genes in
Stage-specific expressions of four different ribonuclease H genes in

... The human pathogen of the genus Leishmania cause worldwide morbidity and infection of visceral organs by some species may be lethal. Lack of rational chemotherapy against these pathogens dictates the study of differential biochemistry and molecular biology of the parasite as compared to its human ho ...
A homozygous double mutation in SMN1
A homozygous double mutation in SMN1

... insufficiency, and was clinically diagnosed with SMA type I. A spinal cord magnetic resonance image (MRI) indicated a normal appearance; however, DNA sequencing of the SMN genes in this patient revealed two different mutations: an eight-base-pair duplication in exon 1 (c.48_55dupGGATTCCG; p.Val19fs* ...
Genetics of Hemophilia
Genetics of Hemophilia

... nucleotides in a single strand, not double like DNA. RNA uses the base uracil in place of thymine.) To copy the gene message, RNA polymerase (an enzyme) partially separates the two strands of DNA so that RNA nucleotides can line up along one strand of DNA to form a complementary copy. ...
Transcription
Transcription

... common s factor in E. coli is s70. 2. Binding of the s factor converts the core RNA pol into the holoenzyme. 3. s factor is critical in promoter recognition, by decreasing the affinity of the core enzyme for non-specific DNA sites (104) and increasing the affinity for the corresponding promoter 4. s ...
Prediction and investigation of novel proteins in DNA double
Prediction and investigation of novel proteins in DNA double

... Percentages of S. pombe, C. elegans and H. sapiens pairs in which both partners share the same GO SLIM annotation as well as third party interactions___________________________________________________51 ...
Neonatal Hyperbilirubinemia and Organic Anion Transporting
Neonatal Hyperbilirubinemia and Organic Anion Transporting

... hepatic enzyme levels, congenital hypothyroidism, sepsis, urinary tract infections, insufficient feeding (> 10% loss of birth weight), dehydration, blood extravasations, a history of hypoxia, polycythemia or a metabolic disease, ABO or Rh incompatibility, G6PD enzyme deficiency, or a diabetic mother ...
Jigsaw handout - the Biology Scholars Program Wiki
Jigsaw handout - the Biology Scholars Program Wiki

... RNA polymerase binds to initiate transcription. When lac repressor binds to the operator site, RNA polymerase cannot bind to the promoter, i.e. lac repressor blocks transcription by blocking the promoter. In the absence of lactose, lac repressor binds to the DNA. When lactose is present, it is taken ...
Curr. Microbiol. 42
Curr. Microbiol. 42

... PCR analysis for cry7 genes was performed on four B. thuringiensis standard strains, as well as on 27 B. thuringiensis field isolates, which have previously been found positive to a pair of universal primers (Un7,8 for cry7 and cry8 groups) [2]. Among the standard strains, only B. thuringiensis subs ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.

... P22pro-I and P22pro-3 are specialized transducing derivatives of phage P22 that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we fo ...
Localization and structural analysis of the ribosomal RNA operons of
Localization and structural analysis of the ribosomal RNA operons of

... Isolation and subcloning of the rRNA operons In order to identify the rRNA genes, purified rRNA (10) was used to probe a lambda bank consisting of R. sphaeroides chromosomal DNA. One recombinant lambda phage was identified and appeared to contain a portion of an rRNA operon (P. Hallenbeck and S. Kap ...
Array-based sequencing of filaggrin gene for
Array-based sequencing of filaggrin gene for

... Visual inspection of identified null-mutations was performed using the Integrated Genome Viewer (Broad Institute; Figure 1E) (Robinson et al. 2011). In an optional pipeline step, the coordinates of identified variants can also be converted to the FLG gene 10-repeat reference sequence that is found i ...
The genomic landscape of meiotic crossovers and gene
The genomic landscape of meiotic crossovers and gene

... eLife digest Most living organisms package their DNA into bundles called chromosomes. These chromosomes generally form pairs, with each chromosome in the pair containing the same number of genes. The genes also come in the same order, but the exact sequence of DNA bases within the genes can be diffe ...
Control of DNA excision efficiency in Paramecium
Control of DNA excision efficiency in Paramecium

... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
DNA Methylation Analysis
DNA Methylation Analysis

... bead-bound probes are used to detect the presence of T or C by hybridization followed by single-base extention with a labeled nucleotide. ...
From the Department of Zoology, University of
From the Department of Zoology, University of

... stable biochemically, and has a very interesting molecular structure which has suggested a useful model for speculations on the process of replication of genes and chromosomes (15). The non-histone protein and ribonucleic acid (RNA) on the other hand vary greatly in amount and this variation is clos ...
Educational Item Section Clinical findings in chromosome aberrations in Oncology and Haematology
Educational Item Section Clinical findings in chromosome aberrations in Oncology and Haematology

... Normalization (horizontalization) of eye position (Down syndrome). Loss of deep palmar furrows (trisomy 8). ...
Ribotyping of Clostridium perfringens from industrially produced
Ribotyping of Clostridium perfringens from industrially produced

... ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl. perfringens varied between 12 and 17. Typical ribotype patterns of Cl. perfringens were as follows: 7–11 bands between 23 kb and 4Æ3 kb, 0–4 bands between 4Æ3 kb and 2Æ0 kb and ...
Databases_what_and_w..
Databases_what_and_w..

... • Proteins come from the DNA via RNA. • Changes in DNA cause changes in proteins. • Changes in proteins cause changes in phenotypes. ...
mMESSAGE mMACHINE® Kit User Guide
mMESSAGE mMACHINE® Kit User Guide

... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
Choice of laxatives and colonoscopic preparation in pregnant
Choice of laxatives and colonoscopic preparation in pregnant

... less common as compared to other osmotic laxatives[5]. Another similarity common to both g roups is the avoidance of phosphosoda preparations. This may be related to the fact that in published studies, newborns were shown to manifest bone demineralization and bone growth failure because of maternal ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH

... and comparison of two repeats indicated 87% nucleotide and 83% amino acid identity (6). The existence of the long homologous repeats suggests that a possible mechanism for the generation of the CRI allotypes may be the addition or deletion of these structural units. The present study addresses this ...
Active Management of the Third Stage of Labor by Skilled Birth
Active Management of the Third Stage of Labor by Skilled Birth

... It is with a tremendous sense of accomplishment and joy that I present this dissertation as partial fulfillment of the requirements for the degree of Doctor of Education. This endeavor would not have been completed without the help of a number of people whom I would like to formally acknowledge. I g ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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