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The new genes of rice: a closer look
The new genes of rice: a closer look

... detail, with the help of experiments, to trap coding DNA and identify the non-coding DNA [21]. Second, in some cases, higher scores were given to rice gene candidates if the same prediction had already been made via a different method or by a different group. Such strategies could initiate a snowbal ...
DNA - CS.Duke
DNA - CS.Duke

... the  cutAndSplice  method.   The   code   given   to   you   will   pop-­‐up   a   file-­‐dialog   box   —   when   run   you   can   use   this   to   navigate   to   either  ecolimed.dat,   which   is   a   smaller   strand  of  DNA   ...
Studying copy number variations using a nanofluidic platform
Studying copy number variations using a nanofluidic platform

... spectrum of human genetic variation but also to assess the significance of such variation in disease-association studies. The first human CNV map was constructed from a study of 270 normal individuals with a total of 1447 CNV regions in the whole genome (9); more than 15 000 CNVs have been found in th ...
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here - Science in School

... 9. Can you think of reasons why, if you are studying more distantly related organisms, it is better to compare amino acid sequences than DNA sequences? Looking at the amino acid sequences gives a functional meaning to the mutations, which DNA sequences do not provide. For example, silent mutations i ...
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Lecture 2 4285 2015 - Scheid Signalling Lab @ York University
Lecture 2 4285 2015 - Scheid Signalling Lab @ York University

... the maternally expressed proteins are made • Consequence: UBE3A is ok, but other genes in the region are not expressed…Prader-Willi syndrome ...
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PERL - unimore.it

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Genotyping of Ryanodine receptor 1 (RYR1) gene associated with
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unit-2 genetics of prokaryotes and eukaryotic
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... As with so many organisms, the occurrence of mutations provides the tools to learn about such things as  The function of the gene;  Its location in the DNA molecule (mapping). ...
Microsoft Word 97
Microsoft Word 97

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BLOOM HELICASE (and BLOOM SYNDROME)
BLOOM HELICASE (and BLOOM SYNDROME)

...  Many types of mutations can occur: missense, frameshift, nonsense, splice-site, etc..  Most common mutation is delATCTGA/insTAGATTC @ position 2281 which is known as a blmAsh mutation ...
Specialized Transduction
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MayerFrankiPoster
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... Our group had genetically modified the cyanobacterium Synechococcus sp. PCC 7002 to produce isoprene. However, for this ‘photo-isoprene’ to become a marketable option for replacing petroleum products, there are many more modifications to be made. One of these involves the gene cpcB that codes for a ...
Fighting the good cause: meaning, purpose
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2011_InstructorSlidesR
2011_InstructorSlidesR

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... of genes, the relationship of genes to chromosomes, and the key differences between DNA and RNA. Here, I’ve included words. A good minute sketch map will need few words, because the maker of the sketch will understand the relationships indicated by lines or arrows based on the sketches. ...
The Bacillus subtilis clpC operon encodes DNA
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... genome sequencing project, suggesting that clpC is the fourth gene of an operon containing six open reading frames (Ogasawara et al., 1994). We recently showed that the six genes are cotranscribed as an operon, preceded by two promoters. One resembles promoters recognized by the vegetative RNA polym ...
Expression of E. coli Phosphofructokinase Gene in an Autotrophic
Expression of E. coli Phosphofructokinase Gene in an Autotrophic

... by the number of transconjugants divided by that of recipients. Plasmids in A. thiooxidans were isolated by the method described by Birnboim and Doly [3], with slight modification (lysozyme was omitted in solution I). A. thiooxidans cells were washed with solution I at least twice before isolation o ...
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Prokaryotes powerpoint
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... • OBLIGATE AEROBES(need oxygen to survive) • OBLIGATE ANAEROBES (killed by oxygen) • AEROTOLERANT(don’t use oxygen, but survive it). ...
The Effects of varying pH on Plasmid Transfer
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... integration is a very simple way to mobilize other genetic material. The F plasmid of Escherichia coli is an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are cal ...
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... tracking dyes (a red dye and an yellow dye) that separate during electrophoresis. In a 1 % agarose gel, the red dye migrates at the same rate as 600 bp DNA fragment and the yellow dye migrates faster than 20 bp. The dyes do not interfere with most downstream enzymatic applications, however it is rec ...
Genetics PPT
Genetics PPT

... Success Criteria: I can describe the factors that control the inheritance of traits in organisms. Warm Up: 1. List at least 3 different characteristics that you possess. This may include skills or physical features. 2. For each characteristic listed, describe where it came from. ...
INTRODUCTOR Y BIOTECHNOLOGY (ABG 504) THEORETICAL MODULE  BY
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... inheritance. In 1928, Frederick Griffith discovered the phenomenon of transformation in which he reported that dead bacteria could transfer genetic material to "transform" other still-living bacteria. Sixteen years later, in 1944, Oswald Theodore Avery, Colin M cLeod and M aclyn M cCarty identified ...
Genome - people.iup.edu
Genome - people.iup.edu

... 6.8 Proteomics and the Interactome • Generates pattern of spots-each spot is an individual protein • Individual spot can be cut out and studies • Mutant and wild type cells can be compared to study protein function ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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