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USB® Thermo Sequenase Cycle Sequencing Kit
USB® Thermo Sequenase Cycle Sequencing Kit

... Electrophoresis equipment—While a standard, non-gradient sequencing gel apparatus is sufficient for much sequencing work, the use of field-gradient (‘wedge’) or salt-gradient gels will allow much greater reading capacity on the gel(13, 14). A power supply offering constant voltage operation at 2000V ...
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... Two scientists, Douglas Cavener and Morris Agaba, conducted the study. Cavener and Agaba compared genomes of giraffes, okapi, and 40 other animals including humans. The scientists wanted to figure out what genes might give giraffes their qualities. The okapi and giraffe genomes are very similar. Ther ...
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CHEM642-07 Powerpoint
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Greedy Algorithms - University of Illinois at Urbana
Greedy Algorithms - University of Illinois at Urbana

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FEBS Letters
FEBS Letters

... Bacillus subtilis and Synechocystis sp. PCC 6803 was performed. This led us to design suitable oligonucleotides (1for, 1rev; see Section 2) to amplify a 1085 bp segment of S. leopoliensis SAUG 1402-1 DNA in a PCR reaction. The deduced amino acid sequence of the sequenced segment shared 51.3% identit ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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