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Histones
Histones

... In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g. plant seeds, Drosophila embryos), in the entire tis ...
Clark: Biotechnology, 2nd Edition Chapter 2: DNA, RNA, and Protein
Clark: Biotechnology, 2nd Edition Chapter 2: DNA, RNA, and Protein

... a. RNA polymerase finds a 3’ UTR. b. When the first AUG in a template is recognized. c. An open reading frame is discovered. *d. RNA polymerase recognizes a promoter sequence, binds to the DNA, and synthesizes RNA from the 5’ end towards the 3’ end of the new piece. 3. The sequence of mRNA will be t ...
5.2.3 Genomes and Gene Technologies
5.2.3 Genomes and Gene Technologies

... • Genomic sequencing to find the function of genes • Genetic engineering to make chemicals, GM organisms and for xenotransplantation • Gene therapy for treatment of diseases ...
PART
PART

... c. Sequences of 3 bases provides 64 (43) combinations, enough to code for the 20 amino acids. d. Later work confirmed the triplet coding sequence with redundancy. (Table 5.3) e. DNA is stable but subject to chemical and radiation damage. f. Excision repair uses enzymes to separate pyrimidines covale ...
My Genetic Profile Worksheet
My Genetic Profile Worksheet

... • Each DNA cluster will be strongly attracted to any cDNA made from complimentary mRNA strands. For example: DNA strands with the base sequence TTCAGGCAG will be attracted to any cDNA strands with the sequence AAGTCCGTC. In other words each DNA cluster will be attracted to cDNA that were made using ...
Document
Document

... dish. Knowing how many colonies are on the plate and how much the culture was diluted makes it possible to calculate the concentration of cells in the original culture. ...
Name_____________________________________ Which is the
Name_____________________________________ Which is the

... bind adhesion motifs on the underlying ECM. This binding leads to clustering of such surface receptors to form a specific type of anchoring junction called ____________________. Adhesion proteins at this anchoring junction link integrins to _____________________, which polymerize and bundle into str ...
Chapter 14 Human Genetics
Chapter 14 Human Genetics

... X-Chromosome inactivation • In females, most genes in one of the X chromosomes are switched off forming a region in the nucleus known as a bar body ▫ In calico cats, a gene that controls the color of coat spots is on the X chromosome ▫ In different parts of the body, different X chromosomes are swi ...
Exam #3 (final)
Exam #3 (final)

... 35. Genotypic and phenotypic designations in prokaryotes 36. Mechanisms of gene exchange in prokaryotes 37. Role of plasmids in gene exchange 38. Mating types in bacteria 39. Properties of transformation 40. Bacteria with 2 or more copies of one or more genes 41. Structure and activity of virus part ...
SBI4U- Molecular Genetics
SBI4U- Molecular Genetics

... polypeptide and person. (3 marks) The insertion would not only change the order of the triplets downstream of the insertion but this would create a premature stop codon in the next triplet. This would terminate translation earlier resulting in a severely malfunctional polypeptide made. This would dr ...
What is the difference between allele, gene, and trait?
What is the difference between allele, gene, and trait?

... Gene and allele are basically make us who we are. They are genetic sequences of our DNA. Although gene is a more general term than allele. For example, humans have facial hair, which is determined by gene. Hair can be thick or patchy, and that is determined by allele. Alleles determine different tra ...
ppt - Department of Plant Sciences
ppt - Department of Plant Sciences

... Example: EcoRI • What is the probability of a sequence of DNA in a plant genome having the sequence of bases corresponding to an EcoRI cut site? • Each site can be 4 possible bases (A, T, C, or G), and the EcoRI enzyme requires 6 sites (GAATTC) • The probability of finding a random site in a genome ...
dna replication - MacWilliams Biology
dna replication - MacWilliams Biology

... 1. The tips of chromosomes are known as telomeres. 2. Particularly difficult to copy. 3. Over time, DNA may actually be lost from telomeres each time a chromosome is replicated. 4. Enzyme called telomerase compensates for this problem by adding short, repeated DNA sequences to telomeres  lengthens ...
Genetics Assessment
Genetics Assessment

... in the jellyfish genome. Can scientists, and indeed science students, insert this gene into other organisms? Today you will perform a transformation using a paper model. What is a transformation? Bacteria have an extra piece of DNA that is much smaller than the rest of their genome, called a plasmid ...
Chromosome “theory” of inheritance
Chromosome “theory” of inheritance

... between chromosomes, and – within each chromosome – their order are both invariant. In other words, if we examine chr. 1 (by the way, they are numbered according to size, eXcept for the X), then in every human being, that chromosome will contain the exact same genes (note – I did not say the exact s ...
Alternative Approaches to Molecular Biology
Alternative Approaches to Molecular Biology

... With a circular chromosome, the DNA is continuous – it has no "end". This means that there will always be DNA from which to make the RNA primer for the lagging strand. d) Other organisms have non-coding sequences at the ends of linear chromosomes called telomeres. A telomere is simply a long stretch ...
Brock Genetic Exchange in Bacteria
Brock Genetic Exchange in Bacteria

... Artificial Transformation ...
Topic 6: Genetics Page 1
Topic 6: Genetics Page 1

Protein Synthesis
Protein Synthesis

... “The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins.” ...
Chromosomes
Chromosomes

... Conflict between male and female over allocation of maternal resources to offspring Paternally expressed genes would promote growth, maternally expressed genes should slow it down. ...
Mutations Foldable
Mutations Foldable

... Color the segment of DNA that has been deleted; next to the picture write: Deletions occur when a segment of DNA has been removed Color the segment of DNA that has been inverted; next to the picture write: Inversions occur when a segment of DNA has been flipped Color the segment of DNA that has been ...
Review: Genetics
Review: Genetics

... is responsible for on each chromosome. • Since no two individuals have the exact same genome, biologist can use DNA fingerprinting to identify individuals – For example, if blood, sperm or hair is found at a crime scene, DNA from the tissue can be cut using restriction enzymes and fragments can be s ...
gene expression_hour 1 - study
gene expression_hour 1 - study

... DNA as genetic material… Concepts of transformation Transformation is a types of genetic transfer found in bacteria. Bacteria can take up the externally DNA. ...
2 - Griffith-Avery-McLeod-McCarty SBI4U – Molecular Genetics Date
2 - Griffith-Avery-McLeod-McCarty SBI4U – Molecular Genetics Date

... genetic material was transferred and was responsible for the __________________________ ...
Genetics notes, long version
Genetics notes, long version

... DNA is in the nucleus of the cell. This is why, when we studied organelles, we called the nucleus the control center of the cell, because the DNA holds all of the instructions for making it work. Most of the time the DNA is loose in the nucleus, they call it chromatin because it makes the nucleus lo ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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