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2013 Training Handout
2013 Training Handout

Where Is DNA Found?
Where Is DNA Found?

... Sir Alec Jeffreys is credited with DNA profiling using RFLP. In September of 1984 after years of work, he saw his first series of blots on an X-ray. The technique was first used in forensics, when in 1985 he was asked by police to confirm the rape confession of 17 year old Richard Buckland, who was ...
BIO 110 Survey of Biology QZM 3 QA 150701.1
BIO 110 Survey of Biology QZM 3 QA 150701.1

... a. They are both made of amino acids. b. Their structures contain sugars. c. They are hydrophobic. d. They are large polymers. e. They each consist of four basic kinds of subunits. 5. To what does the term "polypeptide" specifically refer? a. organic molecules linked by dehydration synthesis b. orga ...
A Brief Introduction to Antigen Receptors
A Brief Introduction to Antigen Receptors

... „ The TCR is composed of two transmembrane chains (either αβ or γδ) linked by a disulfide bridge and associates with the CD3 complex. „ The BCR consists of membrane-bound Ig’s. The BCR associates with an ITAMcontaining heterodimer (Igα and Igβ). „ Tyrosine kinases involved downstream of antigen rec ...
14–3 Human Molecular Genetics
14–3 Human Molecular Genetics

... DNA fingerprinting analyzes sections of DNA that have little or no known function but vary widely from one individual to another. Only identical twins are genetically identical. DNA samples can be obtained from blood, sperm, and hair strands with tissue at the base. ...
Down syndrome
Down syndrome

... • Trisomy 21 (95% of all cases): three complete copies in all cells. • Mosaicism (1-2% of cases): three copies in some but not all cells. • Translocation (3-4% of cases): partial copy of chromosome 21 attached to another chromosome. ...
Chapter 11 - Jamestown Public Schools
Chapter 11 - Jamestown Public Schools

... Genetic Engineering Basic Steps of Genetic Engineering continued •Cutting DNA and Making Recombinant DNA Restriction enzymes are used to generate sticky ends. Sticky ends allow DNA fragments from different organisms to join together to form recombinant DNA. •Cloning, Selecting, and Screening Cells R ...
Cystic Fibrosis treatment and genetic screening
Cystic Fibrosis treatment and genetic screening

... treatable with gene therapy 7. Process where RNA copies DNA 8. Loop of DNA from bacteria used in gene therapy 9. This protein, needed to regulate ion movement, is faulty or absent in people with cystic fibrosis 11. Can be used as a vector, but needs to be disabled first 12. Liposomes and cell membra ...
slides
slides

... •  As a result, all of the cells of the organism will have the genetic alteration present (whether it’s a plant or an animal). A bacterium, since it is a single cell, obviously just contains the altered piece of DNA. ...
DNA ppt notes 2015
DNA ppt notes 2015

...  DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification.  Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions.  Commercial kits are now available for easy PCR reaction setup an ...
DNA Damage and Repair - American Federation for Aging Research
DNA Damage and Repair - American Federation for Aging Research

DNA Damage and Repair - American Federation for Aging Research
DNA Damage and Repair - American Federation for Aging Research

... our cells, except sperm and eggs, contain two copies of each gene. That is, all the genes we need are encoded by 23 chromosomes, but our cells contain a duplicate copy of each chromosome­—or a total of 46. DNA can be damaged in ­several ways. For example, energy 2 | Infoaging Guide to DNA Damage and ...
Topic 5 DNA, mutation and genetic variation study version
Topic 5 DNA, mutation and genetic variation study version

... but most genes actually code for multiple proteins because they join different “exons” the executable or coding portions of a gene together to make different proteins. This process is called alternative splicing. ...
MCQ Sample I- Blue
MCQ Sample I- Blue

... MCQ Sample I- Blue 4. Which of the following is NOT TRUE about bacterial chromosomes? a) the bacterial chromosome is a circular loop of DNA b) the bacterial chromosome is in the cytoplasm of the cell c) the bacterial chromosome is wrapped around histone proteins d) bacteria have the haploid number, ...
Chapter 5 DNA and heritable variation among humans
Chapter 5 DNA and heritable variation among humans

... but most genes actually code for multiple proteins because they join different “exons” the executable or coding portions of a gene together to make different proteins. This process is called alternative splicing. ...
Click www.ondix.com to visit our student-to
Click www.ondix.com to visit our student-to

... Yeast and other similar cells are ideal for such a purpose due to the fact that these cells contain all of the enzymes necessary for normal eukaryotic transcription. Furthermore, recombinant DNA molecules permit integration into the genomes of larger, multicellular organisms such as mammals. A possi ...
Transposons
Transposons

... transcriptase into cDNA the cDNA integrates into the genome Retroelements are found in all eukaryotes such as Tos in rice, copia in animals Ty1 in yeast ...
Recombinant DNA Technology
Recombinant DNA Technology

... environment and incorporates it into its genome. In animal cells, this process is often called transfection, because transformation also means going from normal to cancerous. A cell that is in a state that allows transformation is called competent. ...
From DNA to Protein
From DNA to Protein

...  RNA regulates DNA  Most RNA transcribed is mRNA  mRNA is translated into protein  Some RNA is not translated into protein  tRNA  rRNA  microRNAs Genetic Regulation  All microRNAs identified to date reduce the production of specific proteins  Interfere with mRNAs  Target mRNAs for destruct ...
Molecular Biology DNA Expression
Molecular Biology DNA Expression

Topic 3 notesTEACHER
Topic 3 notesTEACHER

... For years, scientists wondered how cells with identical genetic instructions could be so different. The answer is that each kind of cell uses only some of the genetic information it contains. It uses only the instructions it needs to operate its own kind of cell. For instance, information for build ...
Producing a Recombinant Plasmid, pARA-R
Producing a Recombinant Plasmid, pARA-R

... Producing a Recombinant Plasmid, pARA-R during Lab 2 will be ligated, or bonded together, using DNA ligase, making new recombinant plasmids. These newly formed plasmids will represent recombinant DNA molecules because the four restriction fragments have been recombined in different ways to produce n ...
Eukaryotic Genome: Organization, Regulation, and Evolution
Eukaryotic Genome: Organization, Regulation, and Evolution

... The coordinate regulation of clustered genes in eukaryotic cells is thought to involve changes in the chromatin structure that makes the entire group of genes either available or unavailable for transcription. ...
Biotechnology PPT
Biotechnology PPT

... micropipette and loading wells) TRANSFORMATION Lab 6 Plasmid Lab GEL ELECTROPHORESIS APPLICATION Forensic Lab (Outbreak) in addition to AP Lab 6 Lab Questions Completed in Class Review all Biotech ...
C tudi - DNA to Darwin
C tudi - DNA to Darwin

... in the wrong place on an evolutionary tree (they may look very different when they are in fact closely-related). b. Any examples of convergent evolution could be suggested here, for example, wings in bats and birds, camera-like eyes in primates and cephalopods, streamlined body shapes in dolphins a ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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