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E1. Due to semiconservative DNA replication, one of the sister
E1. Due to semiconservative DNA replication, one of the sister

... E8. Transposon tagging is an experimental method that is aimed at cloning genes. In this approach, a transposon is introduced into a strain, and the experimenter tries to identify individuals in which the gene of interest has lost its function. In many cases, the loss of function has occurred becaus ...
Tailor-Made Poisons for Pathogens
Tailor-Made Poisons for Pathogens

... killing of those cells that encoded the cognate resistance genes, whereas cells that lacked these genes survived. In addition, Citorik et al. found that a customized guide RNA was capable of discriminating between sensitive and resistant strains that differed by a single-nucleotide mutation in the D ...
Karyotypes
Karyotypes

... Biology ...
Bacterial Gene Finding
Bacterial Gene Finding

... Salzberg Genome Biology 2007 8:102 ...
What is a Genome? - Auburn University
What is a Genome? - Auburn University

Transcription and Translation Candy
Transcription and Translation Candy

... Get your original DNA model or rebuild it using the pictures from the DNA structure and replication lab. Transcription is the process of copying the genetic information from DNA into a usable copy of mRNA. Make a model clearly indicating this process make sure to include in your model representation ...
Noncoding DNA - University of Mysore
Noncoding DNA - University of Mysore

Genetic Recombination in Eukaryotes
Genetic Recombination in Eukaryotes

... Recombination between linked genes can be used to map their relative distance on the chromosome. The map unit (1m.u. or 1cM) is defined as a recombinant frequency of 1%. In a dihybrid of linked genes the RF will be between 0% and 50%. ...
homologous structures
homologous structures

... enclosed within a protein coat C. treatable with antibiotics D. single-celled organisms ...
Atypical Patterns of Inheritance
Atypical Patterns of Inheritance

... Autosomal Dominant defects ...
How Do Heritable Changes in Genes Occur?
How Do Heritable Changes in Genes Occur?

... transcription and replication of the genes in which they are present, these lesions are fatal if they go uncorrected. Not surprisingly, therefore, organisms (including ourselves) have a number of different DNA repair systems for eliminating such damage from DNA. But it is the very act of attempting ...
Ch. 5: Presentation Slides
Ch. 5: Presentation Slides

... Replication of Linear DNA • In eukaryotic cell, origins of replication are about 40,000 bp apart, which allows each chromosome to be replicated in 15 to 30 minutes. • Because chromosomes do not replicate simultaneously, complete replication of all chromosomes in eukaryotes usually takes from 5 to 1 ...
Restriction enzymes
Restriction enzymes

... • These enzymes protect bacteria against intruding DNA from other organisms. • They work by cutting up the foreign DNA, a process called restriction. • If foreign DNA enters the bacteria cell the restriction enzyme will cut it up into small pieces. They cut up only certain base pair sequences and th ...
Exam 3 Fa08
Exam 3 Fa08

... Answer only one of the question below. 1. What would happen, in terms of a cell’s ability to do photosynthesis, if a mutation occurred that caused the primary electron acceptor in the water-splitting photosystem (PS2) to become a stronger electron acceptor than the chlorophyll a in the NADPH-produci ...
EEB-20
EEB-20

... increasing morbidity and mortality in populations, the inability to cope with novel and enduring parasites can precipitate population declines and extinction. Maintaining high levels of genetic diversity at immune genes (such as the major histocompatibility complex or MHC gene family) is one way to ...
genotypes
genotypes

... disease like muscular dystrophy, it is important to consider two steps. The first is to determine if the disorder is autosomal or X-linked. • If the disorder is X-linked most of the males will have the disorder because the Y-chromosome cannot mask the affects of an affected X-chromosome. A female ca ...
RPS17 - Diamond Blackfan Anemia Foundation, Inc.
RPS17 - Diamond Blackfan Anemia Foundation, Inc.

... • Genes are segments of DNA that tell your body what proteins to make. There are over 40,000 genes in a human cell: 20,000 on the chromosomes from your mother and a matching set of 20,000 on the chromosomes from your father. (Peas have 10s of thousands of genes too). • Changes in the sequence of the ...
The Genetics of Viruses and Bacteria
The Genetics of Viruses and Bacteria

...  Techniques enabling scientists to manipulate genes and transfer them from one organism to another were developed in microbes.  In addition, viruses and bacteria have unique genetic features with implications for understanding the diseases that they cause.  Bacteria are prokaryotic organisms, wit ...
the genetics of viruses and bacteria
the genetics of viruses and bacteria

...  Techniques enabling scientists to manipulate genes and transfer them from one organism to another were developed in microbes.  In addition, viruses and bacteria have unique genetic features with implications for understanding the diseases that they cause.  Bacteria are prokaryotic organisms, wit ...
Association for Molecular Pathology v. Myriad Genetics, Inc. June 17, 2013 PDF
Association for Molecular Pathology v. Myriad Genetics, Inc. June 17, 2013 PDF

... U.S. Supreme court holds that unmodified genes are products of nature and not patentable On June 13, 2013, the U.S. Supreme Court in Association for Molecular Pathology v. Myriad Genetics, Inc. (referred to as “Myriad”) held that unmodified genes are “products of nature” and not patentable, but that ...
Chapter 7C
Chapter 7C

... Yeast genes can be turned on by histone acetylation and chromatin decondensation (Fig. 7.36b). In the example shown, the GCN4 activator first binds to its UAS upstream of the TATA box of a regulated gene. GCN4-UAS binding recruits a co-activator complex containing the GCN5 histone acetylase to the s ...
DNA Replication, Repair, and Recombination
DNA Replication, Repair, and Recombination

... Copy RNA into DNA by Reverse Transcripase (RT) Similar to Pol I, 5’->3’ synthesis of DNA from RNA template, primed by host tRNA RNA is degraded by RNase H ssDNA directs dsDNA synthesis dsDNA integration into host genome RT: ...
Variant - NC DNA Day
Variant - NC DNA Day

... Every cell in your body has the same genome, except… If you are a mosaic or a chimera, some cells in your body carry a different genome. ...
Mitochondrial Eve
Mitochondrial Eve

... sapiens sapiens from whom all living humans are descended patrilineally. The inherited DNA in the male case is his nuclear Y chromosome rather than the mtDNA. Mitochondrial Eve and Y-chromosomal Adam need not have lived at the same time. For example, Y-chromosomal Adam has been estimated to have liv ...
2013 Training Handout
2013 Training Handout

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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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