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Experiment 1: Determining the presence of E. coli and H. pylori in
Experiment 1: Determining the presence of E. coli and H. pylori in

... therefore it is an ideal indicator of fecal contamination of water. E. coli usually reproduces clonally (without sex, so offspring are genetically identical to parent cells), and clones (also called strains) are host specific. Each clone is genetically different from other clones, so it is possible ...
Genetics: Getting Down to the Basics. Turner syndrome
Genetics: Getting Down to the Basics. Turner syndrome

...  Present in almost every cell  Many genes need to work in pairs, but some only need one functional copy ...
Chapter 10 Practice Test
Chapter 10 Practice Test

... 2. Homologous chromosomes are two chromosomes with identical DNA sequences. 3. During meiosis, chromosome number is reduced through three rounds of cell division. 4. In humans, the ability to roll one's tongue is a dominant trait. Therefore, a tongue roller can only have children who are also tongue ...
Chromosomes - Fall River Public Schools
Chromosomes - Fall River Public Schools

... inherited characteristics. For example, if a gene for eye color is situated at a particular location (locus) on a certain chromosome, then the matching chrom0some will also have a gene specifying eye color at the equivalent locus. However, the eye color code itself might not be the same. For each ho ...
Lecture 11 Biol302 Spring 2012
Lecture 11 Biol302 Spring 2012

...  Cross-linking of adjacent thymine forms thymidine dimers, which block DNA replication and activate error-prone DNA repair mechanisms. ...
slg mock midterm – for practice only
slg mock midterm – for practice only

... b. Each strand of both daughter molecules contains a mixture of old and newly synthesized DNA. c. The two strands of the parental molecule separate, and each functions as a template for synthesis of a new, complementary strand. d. DNA Polymerase III carries out synthesis by extending from the RNA pr ...
Document
Document

...  Asexual organisms don't have back-up copies of genes, sexual organisms have 2 sets of chromosomes and one can act as a back-up if the other is damaged.  Sexual mechanisms, especially recombination, are used to repair damaged DNA - the undamaged chromosome acts as a template and eventually both ch ...
Slide 1
Slide 1

... • Hardy-Weinberg equilibrium is rare for protein-encoding genes that affect the phenotype • However, it does apply to portions of the genome that do not affect phenotype • These include repeated DNA segments ...
Supplementary Information
Supplementary Information

... RNA extracted from each PDAC line and HPDE was subjected to expression profiling on the Agilent 4x44K Whole Genome Microarray, which measures expression levels for over 41,000 transcripts spanning ~30,000 genes. Arrays were conducted according to the Agilent protocol. Briefly, one microgram of total ...
DNA cloning by homologous recombination in Escherichia coli
DNA cloning by homologous recombination in Escherichia coli

... but were surprised because we had anticipated that the major comtarget regions, including the endogenous lacZ gene on the E. coli chropeting product, derived from empty circularization of the linear vecmosome (Fig. 1B, examples 1 & 2), a part of a high-copy plasmid tor, would be dominant. Empty circ ...
Human Genome Case Study
Human Genome Case Study

... “No genetic sample can be totally anonymous,” says Lori large databases such as Framingham and DNA Sciences’ Andrews of the Institute for Science, Law and Technology at Gene Trust, “the property value of any individual genome the Illinois Institute of Technology. “We use DNA in forensics gets smalle ...
jack of diamonds represents the gene for purple pigmentation
jack of diamonds represents the gene for purple pigmentation

... drug), benzo(a)pyrene (found in cigarette and coal smoke), Captan (a fungicide), nitrous oxide (laughing gas), and ozone (a major pollutant when in the lower atmosphere). ...
Synthetic Life - Colin Mayfield
Synthetic Life - Colin Mayfield

... What if a synthetic RNA can be designed to catalyze its own reproduction within an artificial membrane? ...
Promoters - Pennsylvania State University
Promoters - Pennsylvania State University

... The radioactively-labeled DNA probe binds two proteins. Each is sequence specific. ...
Inheritance and Adaptations
Inheritance and Adaptations

... 6. The section of DNA that carries genetic information for a trait is called a(n) __________. ...
Simulating Protein Synthesis to create a CHNOPS! Read the
Simulating Protein Synthesis to create a CHNOPS! Read the

... During transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) nucleotides read and copy the DNA sequence into a single RNA strand. mRNA can leave the nucleus because it is single stranded. mRNA travels to the ribosome where proteins are made. The codons in the mRNA strand ...
Project 1 Concepts in Biology Project 1 Development of a PCR
Project 1 Concepts in Biology Project 1 Development of a PCR

... relatives is discouraged; two genetically similar adults are more likely to give a child two copies of a defective gene. Diseases caused by just one copy of a defective gene, such as Huntington's disease, are rare. Thanks to natural selection, these dominant genetic diseases tend to get weeded out o ...
slg mock midterm – for practice only
slg mock midterm – for practice only

... b. Each strand of both daughter molecules contains a mixture of old and newly synthesized DNA. c. The two strands of the parental molecule separate, and each functions as a template for synthesis of a new, complementary strand. d. DNA Polymerase III carries out synthesis by extending from the RNA pr ...
as a PDF
as a PDF

... feather bulb (old and young) of canary. PCR reaction was performed by a set of novel primers. The results showed two amplified fragments, about 345 and 306 bp for female, and only one 306 bp amplified PCR product for male canaries. This result was shown for each sample. This sex identification syste ...
microarray activity - Blue Valley Schools
microarray activity - Blue Valley Schools

... technology is based on the basic chemistry of DNA. Adenine pairs with thymine and guanine pairs with cytosine. This base complementarity is what allows DNA from cells to bind specifically to known DNA sequences (probes) on a chip. Since a cell expresses hundreds or even thousands of genes at any giv ...
Recombinant DNA Technology
Recombinant DNA Technology

... damaged by mutagens such as toxic chemicals, UV light, or viruses. This process prevents the development of tumors by stopping cells with damaged DNA from undergoing mitosis and passing down this damaged DNA to daughter cells. If it is determined that the DNA can be repaired p53 will activate other ...
Acute diarrhea
Acute diarrhea

... The mitochondria contain their own unique genome. The mitochondrial chromosome consist of a double-stranded circular piece of DNA, which contains 16,568 base pairs(bp) of DNA . All mitochondria are maternally derived because sperm do not usually carry mitochondria into fertilized eggs. The mutation: ...
Teacher Guide: From DNA to Proteins - RI
Teacher Guide: From DNA to Proteins - RI

... amino acid being added, which does not change the structure of the protein at all. Some substitution mutations result in a similar type of amino acid (hydrophobic for hydrophobic or vice versa) being added. This does not greatly affect the protein folding. Some substitution mutations result in a dif ...
Teacher Guide DNA to Protein FINAL-FR - RI
Teacher Guide DNA to Protein FINAL-FR - RI

... amino acid being added, which does not change the structure of the protein at all. Some substitution mutations result in a similar type of amino acid (hydrophobic for hydrophobic or vice versa) being added. This does not greatly affect the protein folding. Some substitution mutations result in a dif ...
High Frequency of Recombination (Hfr)
High Frequency of Recombination (Hfr)

... • 500 cells carry a mutation, – or, one mutation every ~ 100 bases (across a colony), – or, at least a mutation in about every gene (in a colony). ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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