R - Genetics
... SIII-2 transformants in a reaction from which both types may appear (reactions 10 or 11) is somewhat complicated. The complication is due to the necessity of providing in the reaction mixture antibodies against the untransformed recipient cells; these antibodies increase the ultimate frequency of th ...
... SIII-2 transformants in a reaction from which both types may appear (reactions 10 or 11) is somewhat complicated. The complication is due to the necessity of providing in the reaction mixture antibodies against the untransformed recipient cells; these antibodies increase the ultimate frequency of th ...
Chapter 1 Introduction
... and duplications) that are approximately 100 times smaller than those identifiable with conventional karyotyping. The resolution of these arrays depends on the distance between the probes as well as on the sizes of the probes. Initially, the BAC arrays contained approximately one clone per Mb (i.e. ...
... and duplications) that are approximately 100 times smaller than those identifiable with conventional karyotyping. The resolution of these arrays depends on the distance between the probes as well as on the sizes of the probes. Initially, the BAC arrays contained approximately one clone per Mb (i.e. ...
summary_Stickleback_Seg_Dup
... Since the repeats might be an issue, I set up a filter to determine how many of WGACs may be affected. If I use >20hit, 400bp on boundary, hit length <10kb, it affected 30% of WAC pairs. If I use >10hit, and 400bp bound overlap, and hit < 10kb, 60% of WGAC is affected. I then generate the nr space o ...
... Since the repeats might be an issue, I set up a filter to determine how many of WGACs may be affected. If I use >20hit, 400bp on boundary, hit length <10kb, it affected 30% of WAC pairs. If I use >10hit, and 400bp bound overlap, and hit < 10kb, 60% of WGAC is affected. I then generate the nr space o ...
Biosafety Form - University of Idaho
... 2. For Rec DNA/RNA/Synthetic DNA, Biological Agents, and/or “Select Agent & Toxins”: Include a description of research activities & agent manipulation(s). Provide sufficient detail for the requisite(section IV-B-2b-1) IBC risk assessment also see BMBL (Section II). ...
... 2. For Rec DNA/RNA/Synthetic DNA, Biological Agents, and/or “Select Agent & Toxins”: Include a description of research activities & agent manipulation(s). Provide sufficient detail for the requisite(section IV-B-2b-1) IBC risk assessment also see BMBL (Section II). ...
Twin methodology in epigenetic studies
... the full ACE model can be compared with its nested models including the AE model (dropping the C component), the CE model (dropping the A component) and E model (dropping the A and C components). This enables selection of the best fitting and most parsimonious model for a given set of data. Instead ...
... the full ACE model can be compared with its nested models including the AE model (dropping the C component), the CE model (dropping the A component) and E model (dropping the A and C components). This enables selection of the best fitting and most parsimonious model for a given set of data. Instead ...
This article appeared in a journal published by Elsevier. The
... results shown in Fig. 3A clearly reveal that antibody responses were induced significantly by these DNA vaccines before challenge. The responses of antibodies specific to ApxIA or ApxIIA were produced at comparable levels in the group immunized with divalent DNA vaccine. The antibody responses of comm ...
... results shown in Fig. 3A clearly reveal that antibody responses were induced significantly by these DNA vaccines before challenge. The responses of antibodies specific to ApxIA or ApxIIA were produced at comparable levels in the group immunized with divalent DNA vaccine. The antibody responses of comm ...
Genetic population structure of the European anchovy
... Population structure. The marine species appear to have a lower degree of geographic differentiation than continental species. This phenomenon is typically explained by a high dispersal rate due to a planktonic egg and larval phase of many of these species, or adult history stages coupled with an a ...
... Population structure. The marine species appear to have a lower degree of geographic differentiation than continental species. This phenomenon is typically explained by a high dispersal rate due to a planktonic egg and larval phase of many of these species, or adult history stages coupled with an a ...
RNA-Seq analysis
... Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing Technology (typical read length: 30-400 bp depending on technology) RNA-Seq analysis ...
... Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing Technology (typical read length: 30-400 bp depending on technology) RNA-Seq analysis ...
Differential Gene Expression in the Siphonophore
... * E-mail: [email protected] (SS); [email protected] (CWD) ...
... * E-mail: [email protected] (SS); [email protected] (CWD) ...
Natural selection and the function of genome imprinting:
... homologues during DNA repair and recombination in both meiotic and mitotic cells. Maintenance of differences in chromatin structure in somatic cells can sometimes result in the transcription of only one allele at a locus. This pattern of transcription might be selected, in some instances, for reason ...
... homologues during DNA repair and recombination in both meiotic and mitotic cells. Maintenance of differences in chromatin structure in somatic cells can sometimes result in the transcription of only one allele at a locus. This pattern of transcription might be selected, in some instances, for reason ...
1. Cellular control Booklet TN
... 1. similar/same, cells/metabolism; ACCEPT they are all eukaryotic cells 2. similar/same/share, genes or have genes in common; 3. similar/same, (embryonic) development; 4. shared, ancestry/ancestor or all related by evolution; CREDIT due to phylogeny ACCEPT all same kingdom IGNORE ‘they are all anima ...
... 1. similar/same, cells/metabolism; ACCEPT they are all eukaryotic cells 2. similar/same/share, genes or have genes in common; 3. similar/same, (embryonic) development; 4. shared, ancestry/ancestor or all related by evolution; CREDIT due to phylogeny ACCEPT all same kingdom IGNORE ‘they are all anima ...
Plumage Genes and Little Else Distinguish the Genomes of
... divergent warblers that are indistinguishable using traditional molecular markers and that draw substantial conservation attention [3–5]. Whole-genome comparisons show that differentiation is extremely low: only six small genomic regions exhibit strong differences. Four of these divergence peaks occ ...
... divergent warblers that are indistinguishable using traditional molecular markers and that draw substantial conservation attention [3–5]. Whole-genome comparisons show that differentiation is extremely low: only six small genomic regions exhibit strong differences. Four of these divergence peaks occ ...
Lab 1 genomic DNA
... into the organic phase (and interface) whereas nucleic acids partition in the aqueous phase. Usually phenol is used in a 1: 1 mixture with chloroform since deproteinization is more effective when two different organic solvents are used simultaneously. In addition to denaturing proteins, chloroform i ...
... into the organic phase (and interface) whereas nucleic acids partition in the aqueous phase. Usually phenol is used in a 1: 1 mixture with chloroform since deproteinization is more effective when two different organic solvents are used simultaneously. In addition to denaturing proteins, chloroform i ...
Gene expression - Yale University
... that underlie transcription in both bacteria and eukaryotes, and distinguish it from replication. ...
... that underlie transcription in both bacteria and eukaryotes, and distinguish it from replication. ...
... DNA labeling and competitive genome hybridization. DNA was extracted from yeast strains grown from single colonies in GPY (2% glucose, 0.5% peptone, 0.5% yeast extract) following the procedure described by Querol et al. (44). Before the labeling reaction, 10 g of DNA were digested with HinfI (Takar ...
Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... Of the two primers tested on the population of 200 individuals (150-wild type seedlings and 50 mutant seedlings) only the 1650-bp band generated by primer E11 showed significant genetic linkage with gene ‘Luteus-Pa’. This band was linked to the lethal gene at a distance of 38.5 cM, with a 5.72 LOD s ...
... Of the two primers tested on the population of 200 individuals (150-wild type seedlings and 50 mutant seedlings) only the 1650-bp band generated by primer E11 showed significant genetic linkage with gene ‘Luteus-Pa’. This band was linked to the lethal gene at a distance of 38.5 cM, with a 5.72 LOD s ...
A forkhead-domain gene is mutated in a severe speech and
... vitro functional assays19, indicate that inactivation or loss of the forkhead domain is a general mechanism by which mutation of FOX genes can lead to human disease states. Investigations of forkhead-domain mutations associated with autosomal dominant traits suggests that the resulting disorders ar ...
... vitro functional assays19, indicate that inactivation or loss of the forkhead domain is a general mechanism by which mutation of FOX genes can lead to human disease states. Investigations of forkhead-domain mutations associated with autosomal dominant traits suggests that the resulting disorders ar ...
Expansion of the Pseudo-autosomal Region and Ongoing
... progress towards a complete genome sequence (International Human Genome Sequencing Consortium 2001). Eutherian PARs now include only a few genes (e.g., Van Laere et al. 2008), making it unlikely that SA polymorphisms will be found among them, so mammal PARs are no longer likely to be informative abo ...
... progress towards a complete genome sequence (International Human Genome Sequencing Consortium 2001). Eutherian PARs now include only a few genes (e.g., Van Laere et al. 2008), making it unlikely that SA polymorphisms will be found among them, so mammal PARs are no longer likely to be informative abo ...
Genes Identified by Visible Mutant Phenotypes Show Increased Bias
... the dotted line, and features on the reserve strand are displayed below the line. Grey lines mark the extent of gene models with CDS sequences in green and UTR sequences in blue. Orange bars mark the gaps between assembled contigs of the maize genome (stretches of N’s). Red boxes connected by lines ...
... the dotted line, and features on the reserve strand are displayed below the line. Grey lines mark the extent of gene models with CDS sequences in green and UTR sequences in blue. Orange bars mark the gaps between assembled contigs of the maize genome (stretches of N’s). Red boxes connected by lines ...
Analysis of the DNA microarray hybridization images using
... A critical stage of analysis of the DNA array hybridization pictures is determination positions of the spots. Typical hybridization pictures contain, besides desirable objects (spots), some amount of noise. Considering requirement of elimination of noise and performing analysis in reasonable amount ...
... A critical stage of analysis of the DNA array hybridization pictures is determination positions of the spots. Typical hybridization pictures contain, besides desirable objects (spots), some amount of noise. Considering requirement of elimination of noise and performing analysis in reasonable amount ...
Candidatus Mycoplasma haemomuris subsp. ratti
... also aligned using CLUSTAL W (Thompson et al., 1994). Although the dnaK nucleotide sequences showed 84 % similarity between the subgroups (data not shown), the amino acid sequences showed 96 % similarity (Fig. S2). Thus, dnaK analysis, including both nucleotide and amino acid sequences, supported se ...
... also aligned using CLUSTAL W (Thompson et al., 1994). Although the dnaK nucleotide sequences showed 84 % similarity between the subgroups (data not shown), the amino acid sequences showed 96 % similarity (Fig. S2). Thus, dnaK analysis, including both nucleotide and amino acid sequences, supported se ...
#2
... equilibrium (GCn). We plotted the GC-content as a function of the number of substitutions (d), and we measured on this graph the value d12 corresponding to half of the distance between GCi and GCn. We repeated simulations for different values of GCi (0.35, 0.40, 0.45, 0.50, and 0.55), and of GCn (0. ...
... equilibrium (GCn). We plotted the GC-content as a function of the number of substitutions (d), and we measured on this graph the value d12 corresponding to half of the distance between GCi and GCn. We repeated simulations for different values of GCi (0.35, 0.40, 0.45, 0.50, and 0.55), and of GCn (0. ...
Specialized techniques for site-directed mutagenesis in cyanobacteria
... exchanging a wild-type chromosomal allele with an in-frame deletion construct that will not exert polar effects on neighboring genes. In addition, they can be used to introduce specific site-directed mutations within a gene (e.g., a single nucleotide mutation), or for the incorporation of additional ...
... exchanging a wild-type chromosomal allele with an in-frame deletion construct that will not exert polar effects on neighboring genes. In addition, they can be used to introduce specific site-directed mutations within a gene (e.g., a single nucleotide mutation), or for the incorporation of additional ...
The role of variable DNA tandem repeats in bacterial adaptation
... Closer examination of the SSR distribution across the genome shows significant differences in coding and noncoding regions. Because bacterial genomes are more compact than those of eukaryotes, they have comparatively more intragenic than intergenic SSRs. For example, in Escherichia coli K-12, 79.5% ...
... Closer examination of the SSR distribution across the genome shows significant differences in coding and noncoding regions. Because bacterial genomes are more compact than those of eukaryotes, they have comparatively more intragenic than intergenic SSRs. For example, in Escherichia coli K-12, 79.5% ...