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grade 12 life sciences learner notes
grade 12 life sciences learner notes

... Nucleic acids are responsible for the control and transfer of hereditary characteristics and the structure of proteins that are produced during protein synthesis. Each individual organism consists of proteins that are unique to only that organism. This is why organs are not simply transplanted from ...
Analysis of the mitochondrial COI gene and its
Analysis of the mitochondrial COI gene and its

... DNA barcoding as a marker of mtDNA divergence and the robustness of the phylogenetic signal. Ideally, a region should be chosen to simultaneously maximize all of these factors; however, some tradeoffs will be inevitable (Erpenbeck et al., 2006; Roe and Sperling, 2007). Nucleotide substitution patter ...
(HPV) L1 gene DNA possibly bound to particulate aluminum
(HPV) L1 gene DNA possibly bound to particulate aluminum

... sequence analysis. Alignment analysis of a 45–60 bases sequence in the hypervariable region of the L1 gene excised from the computergenerated base-calling electropherogram was performed against various standard HPV genotype sequences stored in the GenBank, using the on-line BLAST system to validate ...
final1-eu-marie-curie-final-report
final1-eu-marie-curie-final-report

... assay. Studies by Gerwick and co-workers on the near complete genome of Moorea producens (L. majuscula), assert that the cyanobacterium does not contain niFH genes in its genome but is endowed with substantial amount of genes for microbial association. In our study we hypothesized that if any genes ...
Ensembl Variations
Ensembl Variations

... situation in which some combinations of SNP alleles occur more or less frequently in a population than would be expected from a random formation of haplotypes from alleles based on their frequencies ...
l Saccharomyces cerevisiae as a Genetic Model Organism
l Saccharomyces cerevisiae as a Genetic Model Organism

... Saccharomyces strains are unable to synthesize. In addition, rich medium provides many macromolecular precursors such as amino acids and nucleotides that wildtype Saccharomyces strains are able to synthesize if necessary. A sugar or other carbon energy source must be added, such as glucose (dextrose ...
A novel de novo frame-shift mutation of the EDA gene in a
A novel de novo frame-shift mutation of the EDA gene in a

... two markers on chromosome X suggested that the EDA gene was responsible for the disease phenotype (data not shown). Direct DNA sequence analysis identified a novel 1-bp insertion, c.573–574insT, in exon 4 of EDA (GenBank accession number NM_001399) (Fig. 2). This insertion resulted in a Table 1 Prim ...
Genome Evolution Due to Allopolyploidization in Wheat
Genome Evolution Due to Allopolyploidization in Wheat

1 X chromosome crossover formation and genome stability in
1 X chromosome crossover formation and genome stability in

... SYBR Green chemistry (SensiMix SYBR Hi-ROX kit, Bioline #QT-605) with transcript-specific primers designed using GETPrime and Primer3 (Supplemental Table 2, (KORESSAAR AND REMM 2007; GUBELMANN et al. 2011; UNTERGASSER et al. 2012). The reference genes rpl-32 and Y45F10D.4 (HOOGEWIJS et al. 2008) wer ...
HSV 1 & 2 - Scioto County Medical Society
HSV 1 & 2 - Scioto County Medical Society

... by detecting a unique nucleic acid base sequence for specific organisms.  The “goal” of molecular testing is no different from classical methods. ...
Mutations in S-Cone Pigment Genes and the Absence of Colour
Mutations in S-Cone Pigment Genes and the Absence of Colour

... Most primates have short-wavelength sensitive (S) cones and one or more types of cone maximally sensitive in the middle to long wavelengths (M/L cones). These multiple cone types provide the basis for colour vision. Earlier experiments established that two species of nocturnal primate, the owl monke ...
Achieving High-Level Functionality through Complexification
Achieving High-Level Functionality through Complexification

... two genes with the same origin must express the same trait, it is possible to know exactly which genes line up using the historical markings (figure 3). Stanley and Miikkulainen (2002c,d) also showed that historical markings can be used to speciate the population, separating incompatible organisms i ...
CHEM642-07 Powerpoint
CHEM642-07 Powerpoint

Restriction Enzyme Digest and Plasmid mapping
Restriction Enzyme Digest and Plasmid mapping

... and RNA It is often incorporated into the gel so that staining occurs during electrophoresis, but the gel can also be stained after electrophoresis by soaking in a dilute solution of ethidium bromide (EB). You must wear disposable gloves during the lab because EB is a mutagen and a suspected carcino ...
DNA Sequence Variation in the Human Y Chromosome: Functions
DNA Sequence Variation in the Human Y Chromosome: Functions

... haploinsufficiency. In normal women with two X’s in each cell, genes on one X are permanently switched off to give women the same dose of X-based genes as that in men. The exceptions to this rule are the X-linked housekeeping genes having their counterparts on the Y chromosome [38]. The housekeeping ...
—1— User Guide © Copyright 2009 Robert C. Edgar, all rights
—1— User Guide © Copyright 2009 Robert C. Edgar, all rights

... (inter) once for the entire genome, then the intra-chromosome simulator (intra) once for each chromosome. This process is called a cycle. The output from one cycle can be used as input to another cycle. It is generally better to run many short cycles rather than one or a few long cycles as longer cy ...
Ch11_lecture - Dr Owen class material
Ch11_lecture - Dr Owen class material

...  mRNA, with a specific base sequence, is used during translation to direct the synthesis of a protein with the amino acid sequence encoded by the mRNA. • Decoding the base sequence of mRNA is the job of tRNA and ribosomes in the cytoplasm. • The ability of tRNA to deliver the correct amino acid to ...
Regulation of biosynthesis and transport of aromatic amino acids in
Regulation of biosynthesis and transport of aromatic amino acids in

... Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram-positive bacteria. A new candidate transcription regulation signal of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococc ...
11.4 How Is The Information In A Gene
11.4 How Is The Information In A Gene

...  mRNA, with a specific base sequence, is used during translation to direct the synthesis of a protein with the amino acid sequence encoded by the mRNA. • Decoding the base sequence of mRNA is the job of tRNA and ribosomes in the cytoplasm. • The ability of tRNA to deliver the correct amino acid to ...
Mammals Differences between the Chicken and Antagonist in the
Mammals Differences between the Chicken and Antagonist in the

... The human IL-1 family contains 11 genes encoded at three separate loci. Nine, including IL-1R antagonist (IL-1RN), are present at a single locus on chromosome 2, whereas IL-18 and IL-33 lie on chromosomes 11 and 9, respectively. There are currently only two known orthologs in the chicken, IL-1b and ...
S-Phase Checkpoint Genes Safeguard High
S-Phase Checkpoint Genes Safeguard High

... each knockout. Oligonucleotide tag sequences present in yeast sometimes differ from their design sequences, presumably due to synthesis errors. Some tags will therefore exhibit poor hybridization, but most often only the UPTAG or DOWTAG of any given construct is affected. To filter out the effect of ...
Supplementary Figures (doc 9746K)
Supplementary Figures (doc 9746K)

... categories are junctions whose start and stop sites have been annotated in the Ensembl gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ star ...
Discovery of Cyclotide-Like Protein Sequences in Graminaceous
Discovery of Cyclotide-Like Protein Sequences in Graminaceous

... a range of plants with the aim of discovering and identifying new peptides belonging to the cyclotide family. To date, cyclotides have been identified in every Violaceae plant screened as well as in a few Rubiaceae species. The Rubiaceae and Violaceae are not closely related phylogenetically, with t ...
ZFX has a Gene Structure Similar to ZFY, the Putative
ZFX has a Gene Structure Similar to ZFY, the Putative

Chromosomal G + C Content Evolution in Yeasts
Chromosomal G + C Content Evolution in Yeasts

... the highest GC3s values and S. mikatae has the lowest values throughout the whole genome. The interspecies differences are greatest in the areas around GC3s peaks, whereas in the troughs, all species have more similar GC3s values. When the GC3s values for individual genes are compared between S. bay ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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