Genome-scale CRISPR pooled screens
... with endothelial or immune cells). It also is possible to deliver different pooled libraries to different populations of cells, capitalizing on recent cell type-specific gene expression data from resources such as the GenotypeeTissue Expression (GTEx) project and the Allen Brain Atlas [31,32]. For ex ...
... with endothelial or immune cells). It also is possible to deliver different pooled libraries to different populations of cells, capitalizing on recent cell type-specific gene expression data from resources such as the GenotypeeTissue Expression (GTEx) project and the Allen Brain Atlas [31,32]. For ex ...
Low X/Y divergence in four pairs of papaya sex
... Deletions on the MSY were shown by the gaps on the X BACs within the aligned regions, such as the regions on X BAC 61H02 from 84 to 89 kb and from 93 to 98 kb and on X BAC 53E18 from 192 to 212 kb. Insertions on the MSY were shown by the gaps on the Y BACs within the aligned regions, such as the reg ...
... Deletions on the MSY were shown by the gaps on the X BACs within the aligned regions, such as the regions on X BAC 61H02 from 84 to 89 kb and from 93 to 98 kb and on X BAC 53E18 from 192 to 212 kb. Insertions on the MSY were shown by the gaps on the Y BACs within the aligned regions, such as the reg ...
Aucun titre de diapositive - Universidad Nacional De Colombia
... UniGene clusters are produced with a supervised procedure: ESTs are clustered using GenBank CDSs and mRNAs data as ”seed” sequences. No attempt to produce contigs or consensus sequences. UniGene uses pairwise sequence comparison at various levels of stringency to group related sequences, placing clo ...
... UniGene clusters are produced with a supervised procedure: ESTs are clustered using GenBank CDSs and mRNAs data as ”seed” sequences. No attempt to produce contigs or consensus sequences. UniGene uses pairwise sequence comparison at various levels of stringency to group related sequences, placing clo ...
Submitting an RNA-Seq job at PATRIC
... sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly repetitive genomes or in the presence of pseudogenes. HISAT (hierarchical indexing for spliced alignment of transcripts)[3] is a highly efficient system for aligning reads from RNA sequenc ...
... sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly repetitive genomes or in the presence of pseudogenes. HISAT (hierarchical indexing for spliced alignment of transcripts)[3] is a highly efficient system for aligning reads from RNA sequenc ...
Document
... • Long linear polymers of molecules called amino acids • Information for synthesis of proteins is contained in the nucleic acid • Proteins have a variety of functions – Catalysis of chemical reactions (enzymes) – Structural (histones, cytoskeletal proteins) – Regulatory (transcription factors, growt ...
... • Long linear polymers of molecules called amino acids • Information for synthesis of proteins is contained in the nucleic acid • Proteins have a variety of functions – Catalysis of chemical reactions (enzymes) – Structural (histones, cytoskeletal proteins) – Regulatory (transcription factors, growt ...
Using a Single-Nucleotide Polymorphism to Predict
... samples are then boiled to rupture the cell membranes and extract the DNA. As the cells lyse they also release enzymes that degrade nucleic acids (DNA and RNA), but many of these enzymes require metal ions as cofactors (Mg++). The Chelex® resin in the sample tubes binds all the free metal ions prese ...
... samples are then boiled to rupture the cell membranes and extract the DNA. As the cells lyse they also release enzymes that degrade nucleic acids (DNA and RNA), but many of these enzymes require metal ions as cofactors (Mg++). The Chelex® resin in the sample tubes binds all the free metal ions prese ...
Acanthamoeba mitochondrial 16S rDNA sequences: inferred
... primers were designed to sequence across the gene in both directions. Sequencing of direct, or cloned, PCR products was done by manual or automated fluorescent sequencing methods. ...
... primers were designed to sequence across the gene in both directions. Sequencing of direct, or cloned, PCR products was done by manual or automated fluorescent sequencing methods. ...
Assessing the Homogeneity of Plasmid DNA: An Important
... Figure 5. Agarose gel electrophoresis: two untreated plasmid samples and one sample of the plasmid transferred into the oc-form. ...
... Figure 5. Agarose gel electrophoresis: two untreated plasmid samples and one sample of the plasmid transferred into the oc-form. ...
GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway
... in higher eukaryotes maintain core components to maintain overall shape and remodeling activity and also substitute or add on other components with more specialized or tissue-speci c domains. As in yeast, humans contain two distinct remodeling complexes homologous to SWI/SNF and flSC, respectively. T ...
... in higher eukaryotes maintain core components to maintain overall shape and remodeling activity and also substitute or add on other components with more specialized or tissue-speci c domains. As in yeast, humans contain two distinct remodeling complexes homologous to SWI/SNF and flSC, respectively. T ...
Conservation genetics of the endangered grassland earless dragon
... The grassland earless dragon, Tympanocryptis pinguicolla, is listed as endangered throughout its range. A recent taxonomic study based on both morphological and allozyme data elevated pinguicolla from a subspecies of T. lineata to full species status, but the allozyme data showed considerable differ ...
... The grassland earless dragon, Tympanocryptis pinguicolla, is listed as endangered throughout its range. A recent taxonomic study based on both morphological and allozyme data elevated pinguicolla from a subspecies of T. lineata to full species status, but the allozyme data showed considerable differ ...
Tutorial - 1000 Genomes
... The 1000 Genomes Pilot Browser Tutorial http://1000genomes.org/ Updated on 05/05/2011 To facilitate immediate analysis of the 1000 Genomes data by the whole scientific community, the Ensembl genome browser was adopted to display SNP calls made by the project, even before they are integrated into dbS ...
... The 1000 Genomes Pilot Browser Tutorial http://1000genomes.org/ Updated on 05/05/2011 To facilitate immediate analysis of the 1000 Genomes data by the whole scientific community, the Ensembl genome browser was adopted to display SNP calls made by the project, even before they are integrated into dbS ...
The Ingredients for a Postgenomic Synthesis of Nature and Nurture
... structurally- or functionally-defined entities, or even mixed functional-structural entities. Instead, genes are ‘‘things an organism can do with its genome’’ (Stotz, 2006b: 905): they are ways in which cells utilize available template resources to create biomolecules that are needed in a specific p ...
... structurally- or functionally-defined entities, or even mixed functional-structural entities. Instead, genes are ‘‘things an organism can do with its genome’’ (Stotz, 2006b: 905): they are ways in which cells utilize available template resources to create biomolecules that are needed in a specific p ...
Enhancers reside in a unique epigenetic environment during early
... The vast majority of enhancers at hypo-methylated loci are not active in any of the embryonic data sets analyzed if we use H3K27ac as a proxy of enhancer activity. To follow up on this observation, we defined two sets of enhancers based on their DNA methylation: hypo-enhancers (<25 % DNA methylation ...
... The vast majority of enhancers at hypo-methylated loci are not active in any of the embryonic data sets analyzed if we use H3K27ac as a proxy of enhancer activity. To follow up on this observation, we defined two sets of enhancers based on their DNA methylation: hypo-enhancers (<25 % DNA methylation ...
Nucleotide sequence analysis - Bioinformatics Unit
... •BLAST stands for Basic Local Alignment Search Tool. It is used to compare a novel sequence with those contained in nucleotide and protein databases. The emphasis of this tool is to find regions of sequence similarity. These can yield clues about the structure ...
... •BLAST stands for Basic Local Alignment Search Tool. It is used to compare a novel sequence with those contained in nucleotide and protein databases. The emphasis of this tool is to find regions of sequence similarity. These can yield clues about the structure ...
Gene Section FANCE (Fanconi anemia, complementation group E)
... Fanconi anaemia's prognosis is poor; mean survival is 20 years (depending on mutation, treatment): patients die of bone marrow failure (infections, haemorrhages), leukaemia, or androgen therapy related liver tumours. It has recently been shown that significant phenotypic differences were found betwe ...
... Fanconi anaemia's prognosis is poor; mean survival is 20 years (depending on mutation, treatment): patients die of bone marrow failure (infections, haemorrhages), leukaemia, or androgen therapy related liver tumours. It has recently been shown that significant phenotypic differences were found betwe ...
Familial Aortopathy — Gene Panels
... The technology used by Illumina sequencing platforms involves the synthesis of a strand complementary to each of the millions of template strands attached to a solid support, using primers and (reversible) terminator nucleotides labelled with fluorochromes specific to the nucleotide type. The seque ...
... The technology used by Illumina sequencing platforms involves the synthesis of a strand complementary to each of the millions of template strands attached to a solid support, using primers and (reversible) terminator nucleotides labelled with fluorochromes specific to the nucleotide type. The seque ...
Neutral lipid storage disease with subclinical myo
... Cycle exercise and oxidative capacity were determined employing an incremental cycle exercise protocol on a Lode Corival cycle ergometer with respiratory gas exchange measured using a Marquette 1100 medical gas analyzer. Maximal force generation in knee extension and flexion and in elbow flexion and e ...
... Cycle exercise and oxidative capacity were determined employing an incremental cycle exercise protocol on a Lode Corival cycle ergometer with respiratory gas exchange measured using a Marquette 1100 medical gas analyzer. Maximal force generation in knee extension and flexion and in elbow flexion and e ...
RTPrimerDB: the real-time PCR primer and probe database, major
... RTPrimerDB is a public primer and probe database for real-time quantitative PCR (qPCR) applications hosted at the Center for Medical Genetics in the Ghent University Hospital in Gent, Belgium. The database was initially developed to address the problem of the repeated laborious primer design and ass ...
... RTPrimerDB is a public primer and probe database for real-time quantitative PCR (qPCR) applications hosted at the Center for Medical Genetics in the Ghent University Hospital in Gent, Belgium. The database was initially developed to address the problem of the repeated laborious primer design and ass ...
AllBio_DJK
... • What are the local and global effects of this QTL on gene expression? • Identify 12 birds with QQ genotypes on the basis of flanking markers and 12 with qq genotype • Perform microarray analyis using mRNA from ...
... • What are the local and global effects of this QTL on gene expression? • Identify 12 birds with QQ genotypes on the basis of flanking markers and 12 with qq genotype • Perform microarray analyis using mRNA from ...
in Stickler syndrome - Journal of Medical Genetics
... radioactivity and is laborious, tim e consuming, and significantly more expensive e. This PCR based restriction digest assay is a Esimple, rapid, and non-radioactive method wk?iich we have used to identify the mutation in the COL2A1 gene in one pedigree with Stickle]r syndrome. Using this assay, we ...
... radioactivity and is laborious, tim e consuming, and significantly more expensive e. This PCR based restriction digest assay is a Esimple, rapid, and non-radioactive method wk?iich we have used to identify the mutation in the COL2A1 gene in one pedigree with Stickle]r syndrome. Using this assay, we ...
Ribosome reinitiation at leader peptides increases translation of
... distance in known operons has been made. In Escherichia coli the distance between two consecutive genes peaks at around 10 nt. While it might support authors’ hypothesis (and there is a glimpse of such discussion in the text), there is no discussion of other mechanisms putatively involved. Response: ...
... distance in known operons has been made. In Escherichia coli the distance between two consecutive genes peaks at around 10 nt. While it might support authors’ hypothesis (and there is a glimpse of such discussion in the text), there is no discussion of other mechanisms putatively involved. Response: ...
Mismatch Repair Error Implies Chargaff`s Second Parity Rule
... latter as Chargaff’s Second Parity Rule (PR2), respectively. Unlike PR, PR2 is not an exact rule but a statistical one. More specifically, according to a recent comprehensive test in [12], PR2 holds for four of the five types of double stranded genomes: the archeal chromosomes, the bacterial chromos ...
... latter as Chargaff’s Second Parity Rule (PR2), respectively. Unlike PR, PR2 is not an exact rule but a statistical one. More specifically, according to a recent comprehensive test in [12], PR2 holds for four of the five types of double stranded genomes: the archeal chromosomes, the bacterial chromos ...
Scientists have found that memories might be passed down through
... He added: “It addresses constitutional fearfulness that is highly relevant to phobias, anxiety and post-traumatic stress disorders, plus the controversial subject of transmission of the ‘memory’ of ancestral experience down the generations. “It is high time public health researchers took human trans ...
... He added: “It addresses constitutional fearfulness that is highly relevant to phobias, anxiety and post-traumatic stress disorders, plus the controversial subject of transmission of the ‘memory’ of ancestral experience down the generations. “It is high time public health researchers took human trans ...
Genetics projects 2015
... computational and bioinformatics analyses, to integrate findings with our existing genome-wide epigenomic and transcription datasets. These analyses will offer crucial novel insight into the genes and factors that direct the cell fate decisions between endocardial and endothelial cells. The delineat ...
... computational and bioinformatics analyses, to integrate findings with our existing genome-wide epigenomic and transcription datasets. These analyses will offer crucial novel insight into the genes and factors that direct the cell fate decisions between endocardial and endothelial cells. The delineat ...
Table 1
... The evolution of genomic technologies is occurring rapidly and often requires large amounts of source DNA. There is also an expanded desire to analyze smaller numbers of cells for higher resolution studies as well as to take advantage of large numbers of archived samples (eg. FFPE, serum, etc.). To ...
... The evolution of genomic technologies is occurring rapidly and often requires large amounts of source DNA. There is also an expanded desire to analyze smaller numbers of cells for higher resolution studies as well as to take advantage of large numbers of archived samples (eg. FFPE, serum, etc.). To ...