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MOLECULAR CYTOGENETIC ANALYSES IN WHEAT AND
MOLECULAR CYTOGENETIC ANALYSES IN WHEAT AND

... decades many results have been achieved in this field. Basic breeding material with more favourable agronomic properties have been developed carrying genes or chromosome segments from the Aegilops, Secale, Agropyron and Triticum genera. Translocations, and addition and substitution lines developed u ...
#2
#2

... equilibrium (GCn). We plotted the GC-content as a function of the number of substitutions (d), and we measured on this graph the value d12 corresponding to half of the distance between GCi and GCn. We repeated simulations for different values of GCi (0.35, 0.40, 0.45, 0.50, and 0.55), and of GCn (0. ...
Replication Protein A (RPA1a) Is Required for Meiotic and Somatic
Replication Protein A (RPA1a) Is Required for Meiotic and Somatic

... Replication protein A (RPA), a highly conserved single-stranded DNA-binding protein in eukaryotes, is a stable complex comprising three subunits termed RPA1, RPA2, and RPA3. RPA is required for multiple processes in DNA metabolism such as replication, repair, and homologous recombination in yeast (S ...
The ARG9 Gene Encodes the Plastid-Resident N
The ARG9 Gene Encodes the Plastid-Resident N

... the dark. Cosegregation of the slow-growth phenotype with the Arg⫹ trait was observed, suggesting that insertion of the ARG9 gene interrupts a gene controlling respiration. However, neither mutant was deficient for complex I activity, as determined by enzymatic measurement or in-gel staining (data n ...
Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of
Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of

... that the organization of the mitochondrial rRNA genes is quite different from the rRNA genes of the chloroplast and nucleus in maize. In spite of the close eubacterial homology observed in the 18S sequence, the organization of rRNA genes is also different from the prokaryotic prototype. Earlier, Gra ...
A Molecularly Defined Duplication Set for the X Chromosome of
A Molecularly Defined Duplication Set for the X Chromosome of

... transformants where possible. Individual balanced G1 transformed flies were backcrossed to w1118; TM2/TM6C,Sb. A single G2 male was backcrossed to w1118; TM2/TM6C,Sb, and a sibling was used for PCR confirmation of proper integration. Sometimes, transgenic progeny were obtained from a female injected ...
Predicting the Genes Regulated by MicroRNAs via Binding Sites in
Predicting the Genes Regulated by MicroRNAs via Binding Sites in

... i.e. single-stranded regions of the secondary RNA structure (Fig. 2b). However, it is not necessary that the full ‘seed match’ (i.e. mRNA sequence complementary to the seed) be accessible at all times; four nucleotides are often sufficient to nucleate the binding (see Section 4 for more details).[14 ...
Bioinformatics Variant Analysis
Bioinformatics Variant Analysis

... which is more prevalent, and a relatively rarely occurring minor allele. The study of single nucleotide polymorphisms is also important in genotyping in crop and livestock breeding. Single nucleotide polymorphisms may fall within coding sequences of genes, non- coding regions, or in the intergenic r ...
Base composition, speciation, and why the mitochondrial
Base composition, speciation, and why the mitochondrial

... mitochondrial DNA sequence (part of the COI gene) could act as a “species barcode” giving high precision species identification (Hebert et al. 2003a). While the term “barcode” raised eyebrows, and problems still remain (especially with plants; Chase and Fay 2009), its accuracy is now clearly recogni ...
Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting.
Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting.

... Ty1 mutagenesis. Each gene was analyzed using DNA (1 mg) isolated at a primary time point for each selection; the pattern of PCR products was compared with that obtained with the time-zero DNA sample. Secondary time points were used to confirm potential growth defects or to resolve ambiguities ident ...
CHAPTER ONE  INTRODUCTION 1
CHAPTER ONE INTRODUCTION 1

... and De Langhe, 1985, Swennen and Vuylsteke, 2001). Further, growth studies in banana have positively correlated leaf emergence and growth rates to faster growth and bunch weight (Swennen and De Langhe, 1985). In addition, Swennen and De Langhe (1985) observed a positive correlation between leaf emer ...
Doing Machine Learning at the Wellcome Trust Sanger Institute
Doing Machine Learning at the Wellcome Trust Sanger Institute

... My Role at the Sanger Genomic mutation and genetic disease group [Matt Hurles] Structural Variation Copy Number Variation in human populations its role in diseases ...
simultaneous detection of colorectal cancer mutations in stool
simultaneous detection of colorectal cancer mutations in stool

... Summary: Colorectal cancer (CRC) is the second main cause of cancer-related death in the Western world and like many other tumours is curable if detected at an early stage. Current detection options include faecal occult blood testing and invasive direct visualisation techniques such as flexible sig ...
Endogenous Retroviral Elements in Human DNA1
Endogenous Retroviral Elements in Human DNA1

... to infectious retroviruses. Indeed, transposition of LINES has been shown to take place in humans, causing disease by insertional mutation in at least one case (5, 6). Thus retroelements are implicated to be mobile genetic elements with a potential to act as causative agents of disease. Retrovirus-l ...
Gene Section FANCD2 (Fanconi anemia, complementation group D2) Atlas of Genetics and Cytogenetics
Gene Section FANCD2 (Fanconi anemia, complementation group D2) Atlas of Genetics and Cytogenetics

... in the FA pathway, will then interact with other proteins involved in DNA repair, possibly BRCA1; after DNA repair, FANCD2 return to the nonubiquinated form (FANCD2-S). FANCD2co-localizes with BRCA1 in DNA damagedinduced loci and in the synaptonemal complex of meotic chromosomes as well. ...
Bacterial genospecies that are not ecologically
Bacterial genospecies that are not ecologically

... while sv. trifolii is confined to clovers (Trifolium). The ‘nodulation genes’ that define these two distinct host specificities, together with genes responsible for the process of nitrogen fixation, are normally encoded on a plasmid in this species. There are complete published genome sequences of R ...
Restriction Enzymes in Microbiology, Biotechnology and
Restriction Enzymes in Microbiology, Biotechnology and

... “The most far-reaching consequence of the emergence of the recombinant DNA technology has been the great strides made in understanding fundamental life processes and the ability to investigate problems that had previously been unapproachable. Emerging from myriad investigations has been the apprecia ...
Rate Asymmetry After Genome Duplication Causes Substantial
Rate Asymmetry After Genome Duplication Causes Substantial

... National University of Ireland, Maynooth, County Kildare, Ireland Whole-genome duplication (WGD) produces sets of gene pairs that are all of the same age. We therefore expect that phylogenetic trees that relate these pairs to their orthologs in other species should show a single consistent topology. ...
PartTwoAnswers.doc
PartTwoAnswers.doc

... periods, labeled nucleotides can be incorporated during initiation of the short nascent chain as well as the during the elongation and termination. Since the 5’ end was labeled only during longer pulses, it must be the part synthesized first. Thus the direction of chain growth is 5’ to 3. Answer 5.1 ...
Initiation of recombination suppression and PAR formation during
Initiation of recombination suppression and PAR formation during

... non-functional DNA segments from the Y chromosome [3]. Sex chromosomes have evolved independently many times from different autosomes in different lineages [4]. In each lineage, different features are associated with sex-chromosome evolution. Drosophila lacks recombination during male meiosis, so th ...
PPT - Bruce Blumberg
PPT - Bruce Blumberg

... • how to do recombinant DNA manipulations • I may begin each lecture with discussion of an important technique – present a practical introduction to techniques • library construction and use • gene identification • functional analysis – point out some of the pitfalls of various methods and why certa ...
Sequence Heterogeneities Among 16s
Sequence Heterogeneities Among 16s

... We have analyzed what phylogenetic signal can be derived by small subunit rRNA comparison for bacteria of different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons wi ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR

... resistance in cell microorganisms by changing the compound toxic to non-toxic. The bacteria that live in extreme environment have a mechanism of resistance toxic heavy metals such as selenium that only specific bacteria can survive on environment containing selenium. The mechanism of resistance is d ...
pdf
pdf

... periods, labeled nucleotides can be incorporated during initiation of the short nascent chain as well as the during the elongation and termination. Since the 5’ end was labeled only during longer pulses, it must be the part synthesized first. Thus the direction of chain growth is 5’ to 3. Answer 5.1 ...
Pandora - W.W. Norton
Pandora - W.W. Norton

... meme-line. The meme for utopia eventually gives rise to a mutation, the meme for dystopia. This meme-line embodies such works as George Orwell’s 1984, Aldous Huxley’s Brave New World and Ray Bradbury’s Fahrenheit 451. Utopia and dystopia adequately illustrate the concept of the meme-line as well as ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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