`Candidatus Midichloria mitochondrii`, an endosymbiont of the tick
... and Rickettsia. Sequences of the gyrB gene from each of these genera were downloaded from the databases and aligned with the sequence from ‘Candidatus M. mitochondrii’. Phylogenetic inference from gyrB gene sequence alignments clearly showed that ‘Candidatus M. mitochondrii’ is placed within the Alp ...
... and Rickettsia. Sequences of the gyrB gene from each of these genera were downloaded from the databases and aligned with the sequence from ‘Candidatus M. mitochondrii’. Phylogenetic inference from gyrB gene sequence alignments clearly showed that ‘Candidatus M. mitochondrii’ is placed within the Alp ...
1 Combining Gene Expression with Marker Genotypes in Poultry
... specific to a particular tissue, but contains oligonucleotide gene probes for most chicken genes. The pituitary was chosen on the basis of its central role in reproduction and growth for which the two lines are markedly different. In order to compare whether any of the differentially expressed gene ...
... specific to a particular tissue, but contains oligonucleotide gene probes for most chicken genes. The pituitary was chosen on the basis of its central role in reproduction and growth for which the two lines are markedly different. In order to compare whether any of the differentially expressed gene ...
Phat—a gene finding program for Plasmodium falciparum
... Sequencing of the Plasmodium falciparum genome is proceeding apace. Two completely sequenced chromosomes have been published [1,2] as well as the mitochondrion, and substantial amounts of the sequence of other chromosomes are already available [3 – 6]. The two published chromosomes have been annotat ...
... Sequencing of the Plasmodium falciparum genome is proceeding apace. Two completely sequenced chromosomes have been published [1,2] as well as the mitochondrion, and substantial amounts of the sequence of other chromosomes are already available [3 – 6]. The two published chromosomes have been annotat ...
Duplication of an approximately 1.5 Mb DNA segment
... mitotic recombination at the molecular level is restricted to an approximate 150 kb DNA fragment between the APC and MCC genes at chromosome 5q22. We have shown in this study that the same recombination site is involved in the duplication of the smallest overlapping region of approximately 1.5 Mb DN ...
... mitotic recombination at the molecular level is restricted to an approximate 150 kb DNA fragment between the APC and MCC genes at chromosome 5q22. We have shown in this study that the same recombination site is involved in the duplication of the smallest overlapping region of approximately 1.5 Mb DN ...
Pair-wise sequence alignment
... • As we can start a new alignment anywhere the scores cannot be negative. • Trace-back is started at the highest values rather than at the lower right hand corner. • Trace-back is stopped as soon as a zero is encountered. ...
... • As we can start a new alignment anywhere the scores cannot be negative. • Trace-back is started at the highest values rather than at the lower right hand corner. • Trace-back is stopped as soon as a zero is encountered. ...
Module 6: Enzymatic Function
... shown in Figure 6.19 are obtained. Results are thus likely to be less specific, and you may need to spend some time determining which is the best fit for your protein. In figure 6.19 the choice of “DNA-directed DNA polymerase” would be most appropriate since Ksed_00020 is not an “RNA-directed DNA po ...
... shown in Figure 6.19 are obtained. Results are thus likely to be less specific, and you may need to spend some time determining which is the best fit for your protein. In figure 6.19 the choice of “DNA-directed DNA polymerase” would be most appropriate since Ksed_00020 is not an “RNA-directed DNA po ...
All research involving recombinant DNA molecules must comply
... ----------------------------------------------------------------------------------------------------------------------------------------Please review this entire form prior to completion, and provide information as requested. Complete the Project Questionnaire on the following page. If you answer al ...
... ----------------------------------------------------------------------------------------------------------------------------------------Please review this entire form prior to completion, and provide information as requested. Complete the Project Questionnaire on the following page. If you answer al ...
Prokaryotes: The First Life on Earth
... Prokaryotes have a small amount of DNA compared to other living organisms.They have a limited number of genes so they can only make a limited number of products from their proteins. If 2 different types of bacterial cells are living very close together, each can use the others products to enhance th ...
... Prokaryotes have a small amount of DNA compared to other living organisms.They have a limited number of genes so they can only make a limited number of products from their proteins. If 2 different types of bacterial cells are living very close together, each can use the others products to enhance th ...
Joanne Ramsey
... Increased cycles and amount of template DNA Scored some fragments as ?No mutation detected ...
... Increased cycles and amount of template DNA Scored some fragments as ?No mutation detected ...
Nematode genome evolution
... an 11-gene region sequenced from P. pacificus chromosome III, 10/11 genes had orthologs on C. elegans chromosome III (Lee et al., 2003). This led Lee et al. to suggest that P. pacificus chromosome III and C. elegans chromosome III shared a common ancestor. If this is true, there must have been a lot ...
... an 11-gene region sequenced from P. pacificus chromosome III, 10/11 genes had orthologs on C. elegans chromosome III (Lee et al., 2003). This led Lee et al. to suggest that P. pacificus chromosome III and C. elegans chromosome III shared a common ancestor. If this is true, there must have been a lot ...
A Blueprint for Change
... ago, most testing was ordered by doctors specializing in medical genetics; today most testing is initiated by non-genetics subspecialties such as oncology, neurology, and cardiology, all of which use genetic testing as a standard diagnostic tool for patient management. However, interpretation of nex ...
... ago, most testing was ordered by doctors specializing in medical genetics; today most testing is initiated by non-genetics subspecialties such as oncology, neurology, and cardiology, all of which use genetic testing as a standard diagnostic tool for patient management. However, interpretation of nex ...
Supplemental Figure and Methods
... We used CAESAR to select 217 genes (1). CAESAR prioritizes candidate genes for complex human traits by semantically mapping trait relevant natural language descriptions to a variety of functional annotation sources. The trait relevant input text used here was four concatenated T2D review articles (2 ...
... We used CAESAR to select 217 genes (1). CAESAR prioritizes candidate genes for complex human traits by semantically mapping trait relevant natural language descriptions to a variety of functional annotation sources. The trait relevant input text used here was four concatenated T2D review articles (2 ...
Specific oligonucleotide primers for detection of endoglucanase
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
Developing expressed sequence tag libraries and
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
Application of a Real Time Polymerase Chain Reaction Method to
... confirm the melting temperature (Tm) of a single target nucleic acid sequence within an unknown sample. Figure 2 displays the melting curves associated with the dissociation analyses from PCRs. The curves obtained from PCR assays using the primer pair, Ricin-F4/R4, SYBR Green I dye, and DNA from mil ...
... confirm the melting temperature (Tm) of a single target nucleic acid sequence within an unknown sample. Figure 2 displays the melting curves associated with the dissociation analyses from PCRs. The curves obtained from PCR assays using the primer pair, Ricin-F4/R4, SYBR Green I dye, and DNA from mil ...
The importance of alternative splicing in the drug discovery process
... comparing the transcriptomes of different cell types or cells under different conditions, as well as for toxicity analyses. In this method, short probes, each representing a gene, are printed on a slide. The cDNA of the tested tissue, fluorescently labeled, is then hybridized to the microarray, and ...
... comparing the transcriptomes of different cell types or cells under different conditions, as well as for toxicity analyses. In this method, short probes, each representing a gene, are printed on a slide. The cDNA of the tested tissue, fluorescently labeled, is then hybridized to the microarray, and ...
The novel mutation of CYP21A2 gene and congenital adrenal
... surgery because of malformation of external genitalia. DNA sequence analysis of CYP21A2 revealed the compound heterozygous mutations (g.6119T>A and g.6699delA) in this patient and her elder sister. Interestingly, the g.6119T>A mutation is associated with a Simple Virilizing (SV) phenotype of CAH, an ...
... surgery because of malformation of external genitalia. DNA sequence analysis of CYP21A2 revealed the compound heterozygous mutations (g.6119T>A and g.6699delA) in this patient and her elder sister. Interestingly, the g.6119T>A mutation is associated with a Simple Virilizing (SV) phenotype of CAH, an ...
serious asthma should focus on the possible confounding role
... the regulated retrotransposon transcriptome of healthy tissue is known, it can be compared with that of asthma patients or additional complex diseases. This will provide unprecedented details about genes and gene expression patterns, and how they relate to the development and/or establishment of com ...
... the regulated retrotransposon transcriptome of healthy tissue is known, it can be compared with that of asthma patients or additional complex diseases. This will provide unprecedented details about genes and gene expression patterns, and how they relate to the development and/or establishment of com ...
Wide-spread polyploidizations during plant evolution Dicot
... Figure 2. Cart oon showing t he rearrangem ent s ind icat ed b y lowercase let t ers in Figure 1. Monocolored chromosomes belong to the WGD Ancestor. Chromosomes in gray boxes are extant L. kluyveri chromosomes. Events encircled by a color correspond to events on branches of the same color in Figure ...
... Figure 2. Cart oon showing t he rearrangem ent s ind icat ed b y lowercase let t ers in Figure 1. Monocolored chromosomes belong to the WGD Ancestor. Chromosomes in gray boxes are extant L. kluyveri chromosomes. Events encircled by a color correspond to events on branches of the same color in Figure ...
Document
... or Equivalent for DNA Sequencing of PCR Products of 1800 Samples” for OliveProject entitled “ Rapid Identification of Olive Cultivars Using DNA Markers” from the sole agents/sole distributors/authorized dealers of the original manufacturers on free delivery basis at NARC, Chak Shahzad, Park Road, Is ...
... or Equivalent for DNA Sequencing of PCR Products of 1800 Samples” for OliveProject entitled “ Rapid Identification of Olive Cultivars Using DNA Markers” from the sole agents/sole distributors/authorized dealers of the original manufacturers on free delivery basis at NARC, Chak Shahzad, Park Road, Is ...
Molecular tools for breeding basidiomycetes
... Summary The industrial production of edible basidiomycetes is increasing every year as a response to the increasing public demand of them because of their nutritional properties. About a dozen of fungal species can be currently produced for food with sound industrial and economic bases. Notwithstand ...
... Summary The industrial production of edible basidiomycetes is increasing every year as a response to the increasing public demand of them because of their nutritional properties. About a dozen of fungal species can be currently produced for food with sound industrial and economic bases. Notwithstand ...
A Tn 10-lacZ-kanR-URA3 Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes.
... cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RADSO, and CDC48 genes of Saccharomyces cerevisiae. This transposon, nicknamed mini-Tn 10-LUK, contains a lacZ gene without efficient transcription or translation ...
... cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RADSO, and CDC48 genes of Saccharomyces cerevisiae. This transposon, nicknamed mini-Tn 10-LUK, contains a lacZ gene without efficient transcription or translation ...
Functional gene groups are concentrated within chromosomes
... like to check whether genes from the same group that belong to the same chromosome tend to be closer than expected. In order to measure this clustering tendency, we calculated for each group i the average distance (in bases), di , between pairs of genes in group i that reside on the same chromosome. ...
... like to check whether genes from the same group that belong to the same chromosome tend to be closer than expected. In order to measure this clustering tendency, we calculated for each group i the average distance (in bases), di , between pairs of genes in group i that reside on the same chromosome. ...
! Mendel`s Law of Independent Assortment
... see that each of these is ¼ of the total number of squares. How do we get the phenotypic results? The sum rule of probability tells us that when the same event can occur in more than one way, we can add the results. Because 1, 2, and 3 all result in unattached earlobes, we add them up to know that t ...
... see that each of these is ¼ of the total number of squares. How do we get the phenotypic results? The sum rule of probability tells us that when the same event can occur in more than one way, we can add the results. Because 1, 2, and 3 all result in unattached earlobes, we add them up to know that t ...