Genes - Gerstein Lab Publications
... ailing families of proteins, but rather the opposite; one might expect more processed pseudogenes for genes that are highly or widely expressed. They have the following features: (i) they lack the introns of the gene from which they are derived; (ii) they tend to have a characteristic polyadenine ta ...
... ailing families of proteins, but rather the opposite; one might expect more processed pseudogenes for genes that are highly or widely expressed. They have the following features: (i) they lack the introns of the gene from which they are derived; (ii) they tend to have a characteristic polyadenine ta ...
Gene targeting by hybridization-hydrolysis process
... The characterization of the genes was monitored by specific amplification of short products differing from each other by their size. H1, H2 and H3 represent the PCR products corresponding to the different genes (from the most abundant to the less abundant in this class). Aliquots were taken every 5 ...
... The characterization of the genes was monitored by specific amplification of short products differing from each other by their size. H1, H2 and H3 represent the PCR products corresponding to the different genes (from the most abundant to the less abundant in this class). Aliquots were taken every 5 ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
... strains is that the recombination pathway is constitutively active in them, causing rearrangements and deletions between the repeat sequences that are found in most BAC and PAC clones. Chi-stimulated recombination. Chi-stimulated recombination provides a way to modify genomes with linear dsDNA in wi ...
... strains is that the recombination pathway is constitutively active in them, causing rearrangements and deletions between the repeat sequences that are found in most BAC and PAC clones. Chi-stimulated recombination. Chi-stimulated recombination provides a way to modify genomes with linear dsDNA in wi ...
Comparison of the separation of Candida albicans chromosome
... karyotypes of organisms refractory to genetic analysis (1,2,3). Candida albicans has attracted particular attention because: 1) It is an important human pathogen and information about its karyotype is of potential diagnostic use; 2) Genetic analysis has been difficult because the organism is natural ...
... karyotypes of organisms refractory to genetic analysis (1,2,3). Candida albicans has attracted particular attention because: 1) It is an important human pathogen and information about its karyotype is of potential diagnostic use; 2) Genetic analysis has been difficult because the organism is natural ...
MayerFrankiPoster
... pharmaceuticals, plastics, and biofuels. One approach to the production of bioproducts is to use cyanobacteria, which are microalgae that derive energy from sunlight and carbon from CO2. Our group had genetically modified the cyanobacterium Synechococcus sp. PCC 7002 to produce isoprene. However, fo ...
... pharmaceuticals, plastics, and biofuels. One approach to the production of bioproducts is to use cyanobacteria, which are microalgae that derive energy from sunlight and carbon from CO2. Our group had genetically modified the cyanobacterium Synechococcus sp. PCC 7002 to produce isoprene. However, fo ...
Altering gene expression by aminocoumarins: the role of DNA
... A mutated gyrB gene (Ile102Ser, Arg144Ile) [23,24] was transduced into strain HG001, resulting in strain HG001 nov142. This strain is resistant to novobiocin and clorobiocin but sensitive towards ciprofloxacin and simocylinone D8 (SD8), with MICs of 80 mg/l, 0.25 mg/l, and 4 mg/l for novobiocin, cip ...
... A mutated gyrB gene (Ile102Ser, Arg144Ile) [23,24] was transduced into strain HG001, resulting in strain HG001 nov142. This strain is resistant to novobiocin and clorobiocin but sensitive towards ciprofloxacin and simocylinone D8 (SD8), with MICs of 80 mg/l, 0.25 mg/l, and 4 mg/l for novobiocin, cip ...
7. glossory - Shodhganga
... Inversion: A chromosomal rearrangement in which chromosome undergoes two breaks and is reconstituted with the segment between the breaks inverted. Inversions are of two types: Paracentric in which both breaks occur in one arm and the centromere is not included during the process, and Pericentric in ...
... Inversion: A chromosomal rearrangement in which chromosome undergoes two breaks and is reconstituted with the segment between the breaks inverted. Inversions are of two types: Paracentric in which both breaks occur in one arm and the centromere is not included during the process, and Pericentric in ...
Real-time Quantification of HER2/neu Gene Amplification by
... The techniques used to evaluate the HER2/neu gene status have included gene-based assays such as Southern and slot blotting, in-situ hybridization (fluorescent and nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast p ...
... The techniques used to evaluate the HER2/neu gene status have included gene-based assays such as Southern and slot blotting, in-situ hybridization (fluorescent and nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast p ...
AthaMap web tools for database-assisted identification of
... sites. A criterion for the conservation of a site is the individual score of a TFBS determined by using Patser (5). In general, only TFBSs with a specific score above a threshold score determined for each matrix were imported into the AthaMap database and are displayed as putative binding sites. A h ...
... sites. A criterion for the conservation of a site is the individual score of a TFBS determined by using Patser (5). In general, only TFBSs with a specific score above a threshold score determined for each matrix were imported into the AthaMap database and are displayed as putative binding sites. A h ...
Gene Order Form - life
... Bio Basic Inc. will deliver to customer 2-4.0ug plasmid DNA containing gene of interest. Plasmid DNA will be in pUC vector and provided in lyophilized powder form. Along with the final gene product, Bio Basic Inc. will provide chromatogram sequencing results, gene report, alignment file, complete nu ...
... Bio Basic Inc. will deliver to customer 2-4.0ug plasmid DNA containing gene of interest. Plasmid DNA will be in pUC vector and provided in lyophilized powder form. Along with the final gene product, Bio Basic Inc. will provide chromatogram sequencing results, gene report, alignment file, complete nu ...
Optimizing Restriction Site Placement for Synthetic
... prized, as they cut the sequence unambiguously in exactly one place. Many techniques for manipulating DNA make use of unique restriction sites [6, 7]. In particular, subcloning is an important method of inserting a new sequence between two different unique restriction sites. Thus a genomic sequence ...
... prized, as they cut the sequence unambiguously in exactly one place. Many techniques for manipulating DNA make use of unique restriction sites [6, 7]. In particular, subcloning is an important method of inserting a new sequence between two different unique restriction sites. Thus a genomic sequence ...
pdf
... either side is 138 kb and ranges from 312 bp to 1.2 Mbp. It is a natural question whether the genes near or overlapping ultra-conserved elements tend to code for similar proteins. We divided the set of 96 genes with ultra-conserved overlap into 3 groups based on where in the gene the overlap occurre ...
... either side is 138 kb and ranges from 312 bp to 1.2 Mbp. It is a natural question whether the genes near or overlapping ultra-conserved elements tend to code for similar proteins. We divided the set of 96 genes with ultra-conserved overlap into 3 groups based on where in the gene the overlap occurre ...
Genetics - Max Appeal!
... What will be the affects of 22q Deletion? The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!) or just a few or have some minor problems, sa ...
... What will be the affects of 22q Deletion? The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!) or just a few or have some minor problems, sa ...
Genetics - Max Appeal!
... What will be the effects of 22q Deletion? The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!), just a few issues or have some minor problem ...
... What will be the effects of 22q Deletion? The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!), just a few issues or have some minor problem ...
The Incompatible Desiderata of Gene Cluster Properties
... gives a simple illustration of how it can be difficult to design a cluster definition that satisfies our basic intuitions about cluster properties. Order: For whole-genome comparison, a cluster is considered ordered if the homologs in the second genome are in the identical or opposite order of the h ...
... gives a simple illustration of how it can be difficult to design a cluster definition that satisfies our basic intuitions about cluster properties. Order: For whole-genome comparison, a cluster is considered ordered if the homologs in the second genome are in the identical or opposite order of the h ...
Chapter 2 Assignment: Genetics
... 35. Insurance companies provide benefits—such as the cost of prescription drugs—to those who take out insurance policies and then become ill. Suppose genetic tests that indicate people’s health risks become widely available. How do you think this will affect the insurance industry? Explain your thin ...
... 35. Insurance companies provide benefits—such as the cost of prescription drugs—to those who take out insurance policies and then become ill. Suppose genetic tests that indicate people’s health risks become widely available. How do you think this will affect the insurance industry? Explain your thin ...
Diversity of DNA methyltransferases that recognize asymmetric
... such an extensive dimer interface strengthens the possibility that M.TTHA0409 functions as a dimer. Gel filtration analysis yielded an apparent molecular weight of 82 kDa, suggesting that M.TTHA0409 exists in a dimeric form in the absence of DNA. Although its structure has been determined, the recog ...
... such an extensive dimer interface strengthens the possibility that M.TTHA0409 functions as a dimer. Gel filtration analysis yielded an apparent molecular weight of 82 kDa, suggesting that M.TTHA0409 exists in a dimeric form in the absence of DNA. Although its structure has been determined, the recog ...
Different physical delivery systems: An important approach for
... efficiency of transduction as well as low cytotoxicity. Compared to electroporation, microinjection requires low protein amounts. This is efficient for transferring recombinant proteins and synthesized peptides with high cost. In contrast with chemical transfection (e.g., liposomes) and viral infect ...
... efficiency of transduction as well as low cytotoxicity. Compared to electroporation, microinjection requires low protein amounts. This is efficient for transferring recombinant proteins and synthesized peptides with high cost. In contrast with chemical transfection (e.g., liposomes) and viral infect ...
Receptor Gene in a Patient with GH Insensitivity Syndrome
... mg) was reverse-transcribed with avian myeloblastosis virus reverse transcriptase (GIBCO BRL, Gaithersburg, MD) in a 20-mL reaction buffer containing dNTPs, random hexamers, RNase inhibitor, and MgCl2 at 37 C for 30 min, followed by heating at 95 C for 5 min. Then, 1–2 mL of the complementary DNA (c ...
... mg) was reverse-transcribed with avian myeloblastosis virus reverse transcriptase (GIBCO BRL, Gaithersburg, MD) in a 20-mL reaction buffer containing dNTPs, random hexamers, RNase inhibitor, and MgCl2 at 37 C for 30 min, followed by heating at 95 C for 5 min. Then, 1–2 mL of the complementary DNA (c ...
Open Access - Cambridge Neuroscience
... little knowledge about its function. RNA expression assays have found FAM43A RNA in a variety of tissues including tissues from the brain, cerebellum and spinal cord. Since FAM43A is not a wellcharacterized gene at this stage, conclusions about its viability as a candidate, and its possible contribu ...
... little knowledge about its function. RNA expression assays have found FAM43A RNA in a variety of tissues including tissues from the brain, cerebellum and spinal cord. Since FAM43A is not a wellcharacterized gene at this stage, conclusions about its viability as a candidate, and its possible contribu ...
Molluscan Studies - Oxford Academic
... within P. hochstetteri lignaria, due to their assumed hybrid origin; however, Powell’s (1979) classification is still followed by some authors (e.g. Trewick et al., 2008; Walker et al., 2008). Many of these P. lignaria subspecies are distributed along the Mokihinui River valley and associated catchme ...
... within P. hochstetteri lignaria, due to their assumed hybrid origin; however, Powell’s (1979) classification is still followed by some authors (e.g. Trewick et al., 2008; Walker et al., 2008). Many of these P. lignaria subspecies are distributed along the Mokihinui River valley and associated catchme ...
Environmental Pollution
... above-mentioned target genes. This method provided a clone library, and sequencing revealed information on new prokaryotes which grouped mainly next to uncultured microorganisms. However, due to the high diversity of approximately 104 ribotypes (wdifferent bacteria) in soils (Torsvik et al., 1990), ...
... above-mentioned target genes. This method provided a clone library, and sequencing revealed information on new prokaryotes which grouped mainly next to uncultured microorganisms. However, due to the high diversity of approximately 104 ribotypes (wdifferent bacteria) in soils (Torsvik et al., 1990), ...
Methods for pattern discovery in unaligned biological sequences
... Pattern discovery in biological sequences is the problem of ®nding patterns that are overrepresented in a set of unaligned DNA or protein sequences of related biological function. Such patterns could correspond to regions of the sequences responsible for the function itself, and could be used later ...
... Pattern discovery in biological sequences is the problem of ®nding patterns that are overrepresented in a set of unaligned DNA or protein sequences of related biological function. Such patterns could correspond to regions of the sequences responsible for the function itself, and could be used later ...