article in press - MRC
... probe cannot readily detect abnormal restriction enzyme fragments. Recently, a novel method for the detection of gene deletions and duplications has been devised: multiplex ligationdependent probe amplification (MLPA) [8]. The method depends on the hybridisation of two short specific oligonucleotide ...
... probe cannot readily detect abnormal restriction enzyme fragments. Recently, a novel method for the detection of gene deletions and duplications has been devised: multiplex ligationdependent probe amplification (MLPA) [8]. The method depends on the hybridisation of two short specific oligonucleotide ...
Efficient Screening of CRISPR/Cas9
... with this e guide, we calculated both the percentage of injected individuals that produced ebony offspring, and also the percentage of e within each individual F1 brood. Sixty-eight fertile P animals were obtained from the mutagenesis experiments, and 43 from the knock-in experiments. Individual P w ...
... with this e guide, we calculated both the percentage of injected individuals that produced ebony offspring, and also the percentage of e within each individual F1 brood. Sixty-eight fertile P animals were obtained from the mutagenesis experiments, and 43 from the knock-in experiments. Individual P w ...
MOTIFS MOTIFSMARTIFAMORIFSMOOTIFSMICIFC
... Motifs may be discovered by comparing homologous genes from different species. For example, by aligning the amino acid sequences specified by the GCM (glial cells missing) gene in man, mouse and D. melanogaster, a pattern was discovered (the GCM motif) that spans about 150 amino acids, and begins as ...
... Motifs may be discovered by comparing homologous genes from different species. For example, by aligning the amino acid sequences specified by the GCM (glial cells missing) gene in man, mouse and D. melanogaster, a pattern was discovered (the GCM motif) that spans about 150 amino acids, and begins as ...
Substitution Rates in a New Silene latifolia Sex
... animals, but they have been found in several phylogenetically distant groups, such as Rumex, Cannabis, and Silene (Westergaard 1958). The genus Silene contains over 500 species. Most of these are gynodioecious or hermaphroditic, but there are two clusters of dioecy that, apparently, evolved independ ...
... animals, but they have been found in several phylogenetically distant groups, such as Rumex, Cannabis, and Silene (Westergaard 1958). The genus Silene contains over 500 species. Most of these are gynodioecious or hermaphroditic, but there are two clusters of dioecy that, apparently, evolved independ ...
Evolution of Gene Order and Chromosome Number in
... all 55 duplicated chromosomal regions arose simultaneously in a whole-genome duplication making yeast, in effect, a degenerate tetraploid. Some regions of the K. lactis genome have gene orders that correspond to an amalgamation of genes from both copies of duplicated regions in S. cerevisiae (Wolfe ...
... all 55 duplicated chromosomal regions arose simultaneously in a whole-genome duplication making yeast, in effect, a degenerate tetraploid. Some regions of the K. lactis genome have gene orders that correspond to an amalgamation of genes from both copies of duplicated regions in S. cerevisiae (Wolfe ...
A comparison of methods for haplotype inference
... individuals can be. On the contrary, a monomorphic marker is not informative because all individuals in the population carry the same allele at this locus. Correlating marker information with phenotypes (disease, quantitative performance for animals like milk production, meat production, etc.) expre ...
... individuals can be. On the contrary, a monomorphic marker is not informative because all individuals in the population carry the same allele at this locus. Correlating marker information with phenotypes (disease, quantitative performance for animals like milk production, meat production, etc.) expre ...
The complete inventory of the yeast Saccharomyces cerevisiae P
... Abstract A total of sixteen open reading frames encoding for P-type ATPases have been identified in the complete genome sequence of Saccharomyces cerevisiae. Phylogenetic analysis distinguishes 6 distinct families. Topology predictions, identification of aminoacid sequence motifs and phenotype analy ...
... Abstract A total of sixteen open reading frames encoding for P-type ATPases have been identified in the complete genome sequence of Saccharomyces cerevisiae. Phylogenetic analysis distinguishes 6 distinct families. Topology predictions, identification of aminoacid sequence motifs and phenotype analy ...
Implications of DNA replication for eukaryotic gene expression
... in a transcriptionally active state are now clear (Fig. 1; and Brown, 1984; Mitchell and Tjian, 1989). The initial direct binding of transcription factors to DNA is rapid, the sequestration of non-DNA binding factors is relatively slow. In vitro, the process of assembling a complete transcription co ...
... in a transcriptionally active state are now clear (Fig. 1; and Brown, 1984; Mitchell and Tjian, 1989). The initial direct binding of transcription factors to DNA is rapid, the sequestration of non-DNA binding factors is relatively slow. In vitro, the process of assembling a complete transcription co ...
Chpt11_TxnPromoters.doc
... DNase I will cut at many (but not all) phosphodiester bonds in the free DNA. The protein-DNA complex is treated lightly with DNase I, so that on average each DNA molecule is cleaved once. The presence of a bound protein will block access of the DNase, and the bound region will be visible as a region ...
... DNase I will cut at many (but not all) phosphodiester bonds in the free DNA. The protein-DNA complex is treated lightly with DNase I, so that on average each DNA molecule is cleaved once. The presence of a bound protein will block access of the DNase, and the bound region will be visible as a region ...
Morris Goodman - National Academy of Sciences
... working now at the DNA level, it was also the vehicle for showing adaptive evolution (Bailey et al. 1992; Bailey et al. 1991; Koop et al. 1986). Recalling this, Morris said, “Using nucleotide sequences of the globins, not just the amino acid sequences, and comparing the non-synonymous changes to syn ...
... working now at the DNA level, it was also the vehicle for showing adaptive evolution (Bailey et al. 1992; Bailey et al. 1991; Koop et al. 1986). Recalling this, Morris said, “Using nucleotide sequences of the globins, not just the amino acid sequences, and comparing the non-synonymous changes to syn ...
Role of Utility and Inference in the Evolution of Functional Information
... Introduction: Functional Information, Knowledge, and Umwelt Information theory explores the statistical properties of signals but entirely ignores the meaning of the transmitted messages. Biosemiotics attempts to fill this gap by studying the meaning of the various kinds of information that are used ...
... Introduction: Functional Information, Knowledge, and Umwelt Information theory explores the statistical properties of signals but entirely ignores the meaning of the transmitted messages. Biosemiotics attempts to fill this gap by studying the meaning of the various kinds of information that are used ...
Disrupting antibiotic resistance propagation by inhibiting
... strains (reviewed in ref. 1), as first described for the Escherichia coli F plasmid by Lederberg and Tatum in 1946 (2). Conjugative DNA transfer is also the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations (reviewed in ref. 3). Indeed, it ...
... strains (reviewed in ref. 1), as first described for the Escherichia coli F plasmid by Lederberg and Tatum in 1946 (2). Conjugative DNA transfer is also the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations (reviewed in ref. 3). Indeed, it ...
Coat Protein of the Ectocarpus siliculosus Virus
... reading frame. Presently, we cannot explain this difference, but processing of a precursor translation product may be a realistic possibility (Kräussl-ich and Wimmer, 1988). In fact, we could identify in the aminoterminal part of the predicted gpl sequence a signal sequence cleavage site, characteri ...
... reading frame. Presently, we cannot explain this difference, but processing of a precursor translation product may be a realistic possibility (Kräussl-ich and Wimmer, 1988). In fact, we could identify in the aminoterminal part of the predicted gpl sequence a signal sequence cleavage site, characteri ...
Cloning and characterisation of a cysteine proteinase gene
... ORF 1. Considering that the Ldccys2 gene from L. (L.) chagasi also presents a C-terminal extension in its 30 UTR (Omara-Opyene and Gedamu, 1997), besides the high identity between this gene and Llacys1 (88%), the nucleotide sequence of the Ldccys2 30 UTR was compared with those from 3A4 and 2A1 clon ...
... ORF 1. Considering that the Ldccys2 gene from L. (L.) chagasi also presents a C-terminal extension in its 30 UTR (Omara-Opyene and Gedamu, 1997), besides the high identity between this gene and Llacys1 (88%), the nucleotide sequence of the Ldccys2 30 UTR was compared with those from 3A4 and 2A1 clon ...
Pourcel et al., Microbiology 2005
... Yersin (1894), strains of biovar Antiqua have been generally isolated from Asia and Africa; Medievalis was found in Central Asia, and Orientalis worldwide. Pestoides are particular strains isolated in Central Asia, and have never been found associated with disease in humans (Anisimov et al., 2004). ...
... Yersin (1894), strains of biovar Antiqua have been generally isolated from Asia and Africa; Medievalis was found in Central Asia, and Orientalis worldwide. Pestoides are particular strains isolated in Central Asia, and have never been found associated with disease in humans (Anisimov et al., 2004). ...
- Lab Fabiana Perocchi
... two expression levels. Intensities measured for the probes specific to the S or Y alleles are expected to be proportional to their expression levels, hS or hY, respectively. Owing to cross-hybridization, probes with sequence highly similar to the other allele yield higher intensities (shown here for ...
... two expression levels. Intensities measured for the probes specific to the S or Y alleles are expected to be proportional to their expression levels, hS or hY, respectively. Owing to cross-hybridization, probes with sequence highly similar to the other allele yield higher intensities (shown here for ...
Divergence Pattern of Duplicate Genes in Protein
... some period of divergent evolution. However, the inference was based on the use of synonymous substitutions between two duplicate copies as a proxy of their age since gene duplication occurred. The accuracy in approximating age of duplication by this means is questionable because it can be greatly b ...
... some period of divergent evolution. However, the inference was based on the use of synonymous substitutions between two duplicate copies as a proxy of their age since gene duplication occurred. The accuracy in approximating age of duplication by this means is questionable because it can be greatly b ...
zChap12_140901 - Online Open Genetics
... 12.1.2 cis- AND trans- REGULATORS In addition to the three protein-coding genes, the lac operon contains short DNA sequences that do not encode proteins, but are instead binding sites for proteins involved in transcriptional regulation of the operon. In the lac operon, these sequences are called P ( ...
... 12.1.2 cis- AND trans- REGULATORS In addition to the three protein-coding genes, the lac operon contains short DNA sequences that do not encode proteins, but are instead binding sites for proteins involved in transcriptional regulation of the operon. In the lac operon, these sequences are called P ( ...
Functional genomics of plant photosynthesis in
... powerful approach for identifying interacting genes, is easily applied using Chlamydomonas. Although nonphotosynthetic mutants can be isolated using plant models such as Arabidopsis or maize, their mutants can only be propagated as heterozygotes, making suppressor analysis much more difficult. The m ...
... powerful approach for identifying interacting genes, is easily applied using Chlamydomonas. Although nonphotosynthetic mutants can be isolated using plant models such as Arabidopsis or maize, their mutants can only be propagated as heterozygotes, making suppressor analysis much more difficult. The m ...
Chapter 18
... NEW AIM: Viruses: Packaged Genes… Lysogenic cycle - After the bacteriophage injects its DNA, it might get incorporated into the bacterial chromosome and is now called a prophage. Now when the bacterial cells replicates, the phage DNA replicates with it. ...
... NEW AIM: Viruses: Packaged Genes… Lysogenic cycle - After the bacteriophage injects its DNA, it might get incorporated into the bacterial chromosome and is now called a prophage. Now when the bacterial cells replicates, the phage DNA replicates with it. ...
Characterisation of a Non-canonical Genetic Code in
... canonical CAA and CAG codons, and by TAA and TAG codons. This is only the fifth lineage of eukaryotes known to employ a non-canonical genetic code in the nuclear genome, but already the fourth lineage where this particular variant is found. The recurrent evolution of this variant code in nuclear gen ...
... canonical CAA and CAG codons, and by TAA and TAG codons. This is only the fifth lineage of eukaryotes known to employ a non-canonical genetic code in the nuclear genome, but already the fourth lineage where this particular variant is found. The recurrent evolution of this variant code in nuclear gen ...
SM 2 Gen Evn
... The first section gives the achievement objectives covered and the achievement standard that could be used to assess the student’s understanding. There is an indication to teachers of the prior learning required by students before they begin the topics. This will vary in some cases depending on the ...
... The first section gives the achievement objectives covered and the achievement standard that could be used to assess the student’s understanding. There is an indication to teachers of the prior learning required by students before they begin the topics. This will vary in some cases depending on the ...
Genes - Gerstein Lab Publications
... ailing families of proteins, but rather the opposite; one might expect more processed pseudogenes for genes that are highly or widely expressed. They have the following features: (i) they lack the introns of the gene from which they are derived; (ii) they tend to have a characteristic polyadenine ta ...
... ailing families of proteins, but rather the opposite; one might expect more processed pseudogenes for genes that are highly or widely expressed. They have the following features: (i) they lack the introns of the gene from which they are derived; (ii) they tend to have a characteristic polyadenine ta ...