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The search for small regulatory RNA
The search for small regulatory RNA

... www.people.vcu.edu/~elhaij/bnfo650 Try Problem 1 with simple oligonucleotides ...
Molecular evolution of swine vesicular disease virus
Molecular evolution of swine vesicular disease virus

... Bush, Roslin EH25 9RG, UK. ...
Two genes from Bacillus subtilis under the sole control
Two genes from Bacillus subtilis under the sole control

... The general stress response of Bacillus subtilis is triggered by a variety of environmental and metabolic stresses which activate the aBtranscription factor. Among the more than 100 genes controlled b y aB.(thecsb genes), the functions identified thus far include resistance to oxidative stress, resi ...
FEMS Microbiology Ecology
FEMS Microbiology Ecology

... Endophytic bacteria are ubiquitous in most plants and colonise plants without exhibiting pathogenicity. Studies on the diversity of bacterial endophytes have been mainly approached by characterisation of isolates obtained from internal tissues. Despite the broad application of culture-independent te ...
$doc.title

... • RNA polymerase I synthesizes rRNA in the nucleolus. • RNA polymerase II synthesizes mRNA in the nucleoplasm. – heterogeneous nuclear RNA (hnRNA) – RNA that comprises transcripts of nuclear genes made primarily by RNA polymerase II; it has a wide size distribution and variable stability. • RNA poly ...
A global view of pleiotropy and phenotypically derived gene function
A global view of pleiotropy and phenotypically derived gene function

... we compared our data to published data sets generated using the homozygous diploid yeast deletion set that assayed similar experimental conditions by a competitive growth/Affymetrix bar-code hybridization method (Winzeler et al, 1999) (Supplementary information). Figure 1 shows a comparison with the ...
Gene Section IGH (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics
Gene Section IGH (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics

... genes are in a more centromeric position. The potentiel genomic IGH repertoire is more limited since it comprises 38-46 functional IGHV genes belonging to 6 or 7 subgroups depending from the haplotypes 23 IGHD, 6 IGHJ, and 9 IGHC genes. Thirty-five IGH genes have been found outside the main locus in ...
Candidate gene resequencing to identify rare, pedigree
Candidate gene resequencing to identify rare, pedigree

... our custom hybridization array. The thresholds used for filtering called variants from sequencing data were determined by comparing common base positions shared with existing GWA data. For genelevel burden tests, variants were filtered for coverage <5fold, quality score <45, call rate < 60 %, MAF > ...
Dolieslager SM, Riggio MP, Lennon A, Lappin DF, Johnston N
Dolieslager SM, Riggio MP, Lennon A, Lappin DF, Johnston N

... S.M.J. Dolieslager et al. / Veterinary Microbiology xxx (2010) xxx–xxx ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
DNA MUTATION, REPAIR, AND TRANSPOSITION

... multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions, on the other hand, only affect a single codon. Therefore, frameshift mutations will have a more deleterious effect than basesubstitution mutations because they affect a larger ...
Module 5: Alternative Open Reading Frame
Module 5: Alternative Open Reading Frame

... downstream start codon was correct. A BLAST search would be done for the sequence in black to compare the e value and score with that obtained for the original amino acid sequence as described in the text. 22. Identify the portions of open reading frames that correspond to the alternative start codo ...
Geminivirus Replication Origins Have a Modular
Geminivirus Replication Origins Have a Modular

... geminiviruses, these proteins can show specificity for replication of their cognate genomes. The A and B genome components are usually only infectious on plants when both are derived from the same geminivirus. Studies carried out with squash leaf curl virus (SqLCV) and tomato golden mosaic virus (TG ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... and L. marthii (4, 7, 17). Of these only, L. monocytogenes (15) and L. ivanovii (1, 18) are considered as pathogens. The pathogenicity is closely associated with a virulence gene cluster, although other genes like those coding for internalines are implicated in pathogenesis too (16). Like L. monocyt ...
SAMPLE LITERATURE Please refer to included weblink for correct
SAMPLE LITERATURE Please refer to included weblink for correct

... can be “tagged” with fluorescent proteins and then expressed in cells. These tags simplify purification because a GFP-labeled protein can be tracked using UV light. The most useful application of GFP is as a visualization tool during fluorescent microscopy studies. By tagging other proteins with GFP, r ...
Maternal and paternal genomes contribute equally to the
Maternal and paternal genomes contribute equally to the

... In animals, maternal gene products deposited into eggs regulate embryonic development before activation of the zygotic genome1. In plants, an analogous period of prolonged maternal control over embryogenesis is thought to occur based on some gene-expression studies2–6. However, other gene-expression ...
Transferrin receptor 2 (TfR2) and HFE mutational analysis in non
Transferrin receptor 2 (TfR2) and HFE mutational analysis in non

... ver, BC, Canada, for genetic testing for HH prior to May 5, 2000. From this database, we selected all patients with a transferrin saturation greater than or equal to 60%, an elevated serum ferritin level, and a negative test result for the C282Y HFE mutation. From the same laboratory, 93 control sam ...
Block 1: Genetics Dr. McKinney Test 1: Transcription (4) The order
Block 1: Genetics Dr. McKinney Test 1: Transcription (4) The order

... i. tRNA and other small RNAs (c) ii. mRNA, miRNA, snRNAs (b) iii. rRNA (a) 1. It’s important to not that all have the same mechanism of action but different types of promoters, and therefore, different transcripts. Also, cell mitochondria have their own RNA polymerases. In contrast, Prokaryotes only ...
Drosophila windpipe codes for a leucine
Drosophila windpipe codes for a leucine

... protein has 677 residues with a potential signal peptide cleavage sequence located between amino acids 20 and 21 (ANA-TP). Based on hydropathy analysis, residues 451– 472 form a transmembrane domain, followed by an acidic-rich ‘stop-transfer’ sequence (KRKC). The predicted extracellular domain conta ...
Distinct effects of 11q aberrations on neuroblastoma with
Distinct effects of 11q aberrations on neuroblastoma with

... Indicated are the stage of disease according to INSS, the age at diagnosis (days), the genomic MYCN, 1p, 11q and 3p status according to FISH results (1, not amplified; n, not deleted; del, deletion; im, imbalance; n.d., not determined), the histological assessment according to Shimada (F, favourable ...
Degree Thesis Adoption of EBPP by DNA: Are Customers
Degree Thesis Adoption of EBPP by DNA: Are Customers

... Finland. But business is business whether it is B2B or B2C, they need to take compensation for the services they have been providing to their clients. All the business organizations deals these kind of process first by sending invoice to the customers and accordingly customers will pay the bills as ...
Widespread expression of the bovine Agouti gene results from at
Widespread expression of the bovine Agouti gene results from at

... (exons 2, 3 and 4). A single 402 bp fragment was amplified from skin samples of each breed and different tissues (brain, heart, kidney, spleen, lung and liver). PCR fragments were purified and subjected to nucleotide sequence analysis to verify that they contain bovine Agouti coding sequences. These ...
Searching for fossil genes
Searching for fossil genes

... are vestigial genes. That is, they were once functional in an ancestral species, but since they were no longer needed they accumulated mutations until they became nonfunctional. In many cases they evolve to the point where a protein can no longer be produced at all. Pseudogenes represent molecular e ...
Additional file 1
Additional file 1

... Normalize any source of signal profile in WIGGLE format then use z-scores to call enriched regions. Combine the signals from different array platform or design, use metaanalysis to call enriched regions. Based on MM-ChIP algorithm. Combine different libraries with different fragment sizes, and use a ...
order code
order code

... Methyl-CpG-binding protein 2 (MeCP2), as the main cause of RTT. MECP2 mutations have also been found in females with atypical RTT and in males with severe neonatal encephalopathy, as well as in patients with clinical features of Angelman syndrome, Klinefelter syndrome, autism, mental retardation, re ...
STPM 2011 - Soalan-Percubaan-STPM
STPM 2011 - Soalan-Percubaan-STPM

... statement best describes restriction enzymes? They are named according to their actions. They cut double-stranded DNA to produce blunt ends They cut double-stranded DNA at specific nucleotide sequences They are used in recombinant DNA technology to join two DNA fragments, ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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