A new monoclonal antibody (CAL2) detects
... Bone marrow (BM) biopsy histology is mandatory for discriminating the different chronic Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) from reactive BM lesions and from each other. This discrimination is in a proportion of cases not possible on purely histological grounds. The ...
... Bone marrow (BM) biopsy histology is mandatory for discriminating the different chronic Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) from reactive BM lesions and from each other. This discrimination is in a proportion of cases not possible on purely histological grounds. The ...
2- pcr primer design and reaction optimisation
... The specific complementary association due to hydrogen bonding of singlestranded nucleic acids is referred to as "annealing": two complementary sequences will form hydrogen bonds between their complementary bases (G to C, and A to T or U) and form a stable double-stranded, anti-parallel "hybrid" mol ...
... The specific complementary association due to hydrogen bonding of singlestranded nucleic acids is referred to as "annealing": two complementary sequences will form hydrogen bonds between their complementary bases (G to C, and A to T or U) and form a stable double-stranded, anti-parallel "hybrid" mol ...
Mapping of the Recessive White Locus and
... typed for 2 new markers (169333023 and 180893258). Figure 1 shows the genotypes of the marker used to determine gene order around the C locus. Based on the recombination between the markers, the most likely gene order and genetic distance were determined as follows: 163386361-(4.0 cM)-ABR631-(7.7 cM ...
... typed for 2 new markers (169333023 and 180893258). Figure 1 shows the genotypes of the marker used to determine gene order around the C locus. Based on the recombination between the markers, the most likely gene order and genetic distance were determined as follows: 163386361-(4.0 cM)-ABR631-(7.7 cM ...
Molecular Plant-Microbe Interactions
... O. viciifolia (Fig. 2; Table 1). These mutants showed a reduced number of nodules on A. cicer plants, compared with the wild-type strain N33. This reduction in the number of nodules was not observed on O. viciifolia plants except for the intergenic nodG-B mutant (JC306), which showed a slightly redu ...
... O. viciifolia (Fig. 2; Table 1). These mutants showed a reduced number of nodules on A. cicer plants, compared with the wild-type strain N33. This reduction in the number of nodules was not observed on O. viciifolia plants except for the intergenic nodG-B mutant (JC306), which showed a slightly redu ...
The coiled-coil of the human Rad50 DNA repair protein contains
... For polymers like DNA, this method yields an average persistence length that is in good agreement with values obtained by other techniques (8). This method has been modified to describe known segments of different persistence length (9). Another method for the determination of overall flexibility of ...
... For polymers like DNA, this method yields an average persistence length that is in good agreement with values obtained by other techniques (8). This method has been modified to describe known segments of different persistence length (9). Another method for the determination of overall flexibility of ...
Construction of consecutive deletions of the Escherichia
... mini-F plasmid, which has an FRT site and is replication defective at 421C. The other is a R6K derivative, which has an FRT site and is replication defective due to the absence the pir gene necessary for replication. In step 1, the suicide plasmid carrying B is introduced into an E. coli strain. The ...
... mini-F plasmid, which has an FRT site and is replication defective at 421C. The other is a R6K derivative, which has an FRT site and is replication defective due to the absence the pir gene necessary for replication. In step 1, the suicide plasmid carrying B is introduced into an E. coli strain. The ...
The Co-Evolution of Genes and Culture Pedigrees and the
... lactose tolerant. They can also be referred to as being lactase persistent, meaning that lactase production persists beyond childhood. (People who no longer produce lactase as adults are called lactase nonpersistent.) Genetic studies suggest that lactose tolerance arose among human populations in th ...
... lactose tolerant. They can also be referred to as being lactase persistent, meaning that lactase production persists beyond childhood. (People who no longer produce lactase as adults are called lactase nonpersistent.) Genetic studies suggest that lactose tolerance arose among human populations in th ...
Yeast as a Model Genetic Organism
... When diploid budding yeast are starved of nutrients, they can undergo meiosis to generate four haploid spores (Figures 1 and 2). Spores are more resistant to the environment than are vegetatively dividing cells. The four spores of a single meiosis are held together in an ascus, or tetrad, surrounded ...
... When diploid budding yeast are starved of nutrients, they can undergo meiosis to generate four haploid spores (Figures 1 and 2). Spores are more resistant to the environment than are vegetatively dividing cells. The four spores of a single meiosis are held together in an ascus, or tetrad, surrounded ...
as a PDF
... (Meyer et al., 1991). However, their capacity to use alternative genetic decoding can be extended to the utilization of ⫹1 frameshifting to express nuclear proteins (Klobutcher and Farabaugh, 2002). It has been estimated that ⬎5% of genes require ⫹1 frameshifting for their expression and although th ...
... (Meyer et al., 1991). However, their capacity to use alternative genetic decoding can be extended to the utilization of ⫹1 frameshifting to express nuclear proteins (Klobutcher and Farabaugh, 2002). It has been estimated that ⬎5% of genes require ⫹1 frameshifting for their expression and although th ...
Chapter 9 Applications of probability
... This means that each of the nucleotides has a probability 1/4 of occurring in any spot. There are 4 × 4 × 4 = 64 possible combinations of three letters, as shown in Table 1. Out of these, four codons (GGT, GGC, GGA, GGG) code for Glycine. The probability that a codon chosen at random codes for Glyci ...
... This means that each of the nucleotides has a probability 1/4 of occurring in any spot. There are 4 × 4 × 4 = 64 possible combinations of three letters, as shown in Table 1. Out of these, four codons (GGT, GGC, GGA, GGG) code for Glycine. The probability that a codon chosen at random codes for Glyci ...
Applied and Environmental Microbiology
... A PCR-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) form I large-subunit genes (cbbL) as a functional marker of autotrophic bacteria that fix carbon dioxide via the Calvin-Benson-Bassham cycle. We constructed two different primer sets, targeting the ...
... A PCR-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) form I large-subunit genes (cbbL) as a functional marker of autotrophic bacteria that fix carbon dioxide via the Calvin-Benson-Bassham cycle. We constructed two different primer sets, targeting the ...
14 Phylogenomic Approach to the Evolutionary Dynamics of Gene
... 2004). Birds may also have fewer protein-coding genes in their genomes than mammals have, with roughly 18,000 in chickens compared with approximately 22,000 in humans (Hillier et al., 2004). By comparing the genomes of chicken with those of humans and pufferfish (Fugu), the difference in gene count ...
... 2004). Birds may also have fewer protein-coding genes in their genomes than mammals have, with roughly 18,000 in chickens compared with approximately 22,000 in humans (Hillier et al., 2004). By comparing the genomes of chicken with those of humans and pufferfish (Fugu), the difference in gene count ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
Genetics_Review_Jeopardy_
... During this process the DNA strands separate and one DNA strand is used to make mRNA, and during this process mRNA is used to produce proteins. What are transcription and translation? ...
... During this process the DNA strands separate and one DNA strand is used to make mRNA, and during this process mRNA is used to produce proteins. What are transcription and translation? ...
Exploring Human Visual Pigments Copyright 2008, Gale Rhodes
... your search, in the colors that signify their BLAST scores, are located for you on the diagram. Notice that there are about 100 proteins (discovered so far, that is) that have 40% or more positives in alignment with red opsin. The opsins are members of the very large family of G protein-coupled rece ...
... your search, in the colors that signify their BLAST scores, are located for you on the diagram. Notice that there are about 100 proteins (discovered so far, that is) that have 40% or more positives in alignment with red opsin. The opsins are members of the very large family of G protein-coupled rece ...
Article PDF
... 2.2. Verification of Convergence. It was shown previously that 50 ns was needed to equilibrate ions near DNA chains.22,23 To verify the validity of this assumption in our simulation, we divided the trajectory into four 50 ns sections and computed radial distribution functions for each section indivi ...
... 2.2. Verification of Convergence. It was shown previously that 50 ns was needed to equilibrate ions near DNA chains.22,23 To verify the validity of this assumption in our simulation, we divided the trajectory into four 50 ns sections and computed radial distribution functions for each section indivi ...
PDF
... to first intron in rice, so our results are similar to those previously reported in Drosophila. In a comparison of two species of Drosophila (D. melanogaster and D. yakuba), Haddrill et al. [13] found that first introns evolve at similar rates to other introns. In rodents and mammals, however, it ha ...
... to first intron in rice, so our results are similar to those previously reported in Drosophila. In a comparison of two species of Drosophila (D. melanogaster and D. yakuba), Haddrill et al. [13] found that first introns evolve at similar rates to other introns. In rodents and mammals, however, it ha ...
De novo DNA cytosine methyltransferase activities in
... 3196 H. Lei and others of development and to identify the gene or genes that encode these activities. Biochemical studies in the past have shown that mammalian DNA methyltransferases purified from somatic cells prefer hemimethylated DNA as substrate as would be expected for a maintenance methyltran ...
... 3196 H. Lei and others of development and to identify the gene or genes that encode these activities. Biochemical studies in the past have shown that mammalian DNA methyltransferases purified from somatic cells prefer hemimethylated DNA as substrate as would be expected for a maintenance methyltran ...
Assembly of additional heterochromatin distinct from centromere
... centromere assembly or centromere inactivation. CENP-A chromatin and centromere components, however, can be reassembled specifically on the ectopic alphoid YAC integration sites that correlate with transcriptional activation on the marker gene on the YAC sites (Nakano et al., 2003). The result indic ...
... centromere assembly or centromere inactivation. CENP-A chromatin and centromere components, however, can be reassembled specifically on the ectopic alphoid YAC integration sites that correlate with transcriptional activation on the marker gene on the YAC sites (Nakano et al., 2003). The result indic ...
Whole Genome Sequencing and foodborne pathogens: a UK
... outbreaks of E. coli O157 PT2 both linked to the consumption of watercress Strains from Outbreak 1 cluster were not closely related to any other UK strain in the Public Health England WGS database suggesting a possible imported source Epidemiologically unrelated isolates on the same branch as the Ou ...
... outbreaks of E. coli O157 PT2 both linked to the consumption of watercress Strains from Outbreak 1 cluster were not closely related to any other UK strain in the Public Health England WGS database suggesting a possible imported source Epidemiologically unrelated isolates on the same branch as the Ou ...
Protein Interactions Limit the Rate of Evolution of
... protein-coding sequences were downloaded based on the most recently released contig assemblies. Ortholog candidates between two species are defined primarily by reciprocal top scores of gapped Blast. This relationship is extended to multiple genomes so that every open reading frame (ORF) from an ass ...
... protein-coding sequences were downloaded based on the most recently released contig assemblies. Ortholog candidates between two species are defined primarily by reciprocal top scores of gapped Blast. This relationship is extended to multiple genomes so that every open reading frame (ORF) from an ass ...
Evolutionary Origin of Recombination during Meiosis
... bacterial cell, whereby nonsister homologous chromosomes (or parts of chromosomes) originating from different parent cells align and undergo genetic recombination. The recombined DNA is passed on to a progeny bacterium. The core genes for meiosis were very likely present in the common ancestor of al ...
... bacterial cell, whereby nonsister homologous chromosomes (or parts of chromosomes) originating from different parent cells align and undergo genetic recombination. The recombined DNA is passed on to a progeny bacterium. The core genes for meiosis were very likely present in the common ancestor of al ...