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Causes, consequences and solutions of
Causes, consequences and solutions of

... Phylogenetic incongruence data has increased the variety of classifications rather than reducing the problem [10, 16]. Thus, despite the rise of phylogenomics [17], many important nodes remain unresolved [18]. In reality, large amounts of data with available efficient algorithms have made immense p ...
Microsoft Word - Mapping-Traits-in-Dogs
Microsoft Word - Mapping-Traits-in-Dogs

Correlation of amino acid preference and
Correlation of amino acid preference and

... (300 amino acids) and medium length (660 amino acids). The analysis indicates a correlation between the viral genome types dsDNA, ssDNA, ssRNA positive strand, ssRNA negative strand and retroid and amino acid preference. We investigated three different models of amino acid preference. The simplest a ...
Chip-seq analysis
Chip-seq analysis

... Effective Genome size: 199400000 (80% of chr1 because we restricted the read mapping to this region). ...
Week 2. DNA isolation and PCR
Week 2. DNA isolation and PCR

... Step 2. Set up your reaction. Pipette in this order: 1) water, 2) PCR mix, 3) primer 1, 4) primer 2, 5) DNA. 2. Briefly spin the tube. 3. Ask the instructor how to label the reaction and where to place it when you have completed the PCR setup. Task 3: Predict the PCR results. Despite the fact that y ...
A Resurrection of B Chromosomes?
A Resurrection of B Chromosomes?

... more recent data suggest that the size of a functional centromere might be only a few hundred kilobases (Nagaki et al., 2004). It has been shown that barley centromeric repeats are neither necessary nor sufficient to establish a centromere (Nasuda et al., 2005). Rapid inactivation of the second cent ...
A candidate prostate cancer susceptibility gene at
A candidate prostate cancer susceptibility gene at

Blue eye color in humans may be caused by a perfectly associated
Blue eye color in humans may be caused by a perfectly associated

... quently, several other linkage and association studies conWrmed the locus and OCA2 as the major contributors to human eye color variation (Sturm et al. 2001; Zhu et al. 2004; Posthuma et al. 2006; Frudakis et al. 2003) and 74% of the variation was estimated to a QTL, linked to OCA2 (DuVy et al. 2007 ...
Bioinformatics
Bioinformatics

Tandem and segmental gene duplication and
Tandem and segmental gene duplication and

... clearly distinguishable clades of NBS-LRR genes, respectively (Figure 1). When the phylogenies were projected on the Arabidopsis physical map, most of the ,40 clusters consisted of genes of the same phylogenetic lineage, suggesting that TANDEM DUPLICATION of these DNA sequences had occurred. However ...
Mutations
Mutations

... • If a gene is a linear set of nucleotides nucleotides, recombination between homologous chromosomes carrying different mutations within the same gene should generate wild type wild-type. • T4 phage as an experimental system: • Can examine a large number of progeny to detect rare mutation events • C ...
A pseudogene cluster in the leader region of the Euglena
A pseudogene cluster in the leader region of the Euglena

... precursor are lost by aquisition of mutational changes, due to imperfect duplication or by other reasons, a l l the genes within the same transcription unit may be unable to function normally and subsequently they became pseudogenes, even 1f some of the genes preserve their sequences necessary to fu ...
The plant genome`s methylation status and response to stress
The plant genome`s methylation status and response to stress

... genetic distance [47]. Similar results were found using A. thaliana accessions [48]. Meiotically heritable epialleles are inherited in a Mendelian fashion but revert at some frequency in subsequent generations ([42,43]; C Pires, pers. comm.). In the context of plant improvement, one desires to gener ...
Chpt20_TxnlRegChromatin.doc
Chpt20_TxnlRegChromatin.doc

... nuclei. This shows that the amount of globin gene DNA in erythroid nuclei is substantially reduced by the DNase I treatment, i.e. the globin gene is sensitive to DNase I in a cell that is expressing it. {To put a finer touch on it, the erythrocytes are descended from cells that were actively express ...
Word - NIEHS SNPs Program - University of Washington
Word - NIEHS SNPs Program - University of Washington

... As a launching point, we will begin our search at the NIEHS SNPs resource. This can be accessed at http://egp.gs.washington.edu/ The NIEHS SNPs Program at the University of Washington is part of the EGP. The EGP is a multi-disciplinary effort focused on exploring the relationships between environmen ...
20060710_GOannotCamp_Stanford
20060710_GOannotCamp_Stanford

... for up-to-date info: http://wiki.geneontology.org/index.php/Reference_Genome_Focus BACKGROUND More and more genomes are being sequenced, but few of these are going to have well funded databases or curators. GOALS:  GO provides the set of reference genome (RG) annotations: 9 genomes, 9 organization ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
Explaining the Likelihood Ratio in DNA Mixture Interpretation

... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
Genetic Analysis of Familial Connective Tissue Alterations
Genetic Analysis of Familial Connective Tissue Alterations

... are detected on the Y chromosome (AC006328) and in multiple loci of 15q21 (AC019294; AC104758; AC136698; AC135995; AC135735; AC127482; AC136704; AC044860; AC126605; AC110291; AC005630; AC010725; AC011295; AC010724; AC012064). In order to design specific primers for single exons all these different p ...
Explaining the Likelihood Ratio in DNA Mixture
Explaining the Likelihood Ratio in DNA Mixture

... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
Evaluation of genomic DNA from paraffin
Evaluation of genomic DNA from paraffin

... separated by a clear space with oedematous fluid and in some cases there is also a diffuse infiltration of subendocardial fibrosis (Tidholm & Jönsson 2005). In a study, 64 of 65 (98%) dogs with confirmed DCM were positive for attenuated wavy fibers. In 147 dogs with other heart disease than DCM only ...
Discovery and classification of ecological diversity in the
Discovery and classification of ecological diversity in the

... However, it is becoming increasingly evident that any particular cut-off value (such as 70%) is arbitrary and not guaranteed to yield groups of bacteria that correspond to real ecological units (82). Also, it is not clear what determines the fraction of genomic segments that anneal in hybridization ...
The Chloroplast trnT–trnF Region in the Seed Plant
The Chloroplast trnT–trnF Region in the Seed Plant

... DNAs were checked by 1% agarose gel electrophoresis with a (kHindIII/EcoRI size marker. The chloroplast tRNALeu(UAA) intron and adjacent IGSs were amplified using primers designed by Taberlet et al. (1991) (Fig. 1 shows their placement). The PCR reaction volume was 25 ll and contained 2.5 ll of Taq 1 ...
Biosafety Protocol Registration Form
Biosafety Protocol Registration Form

Chpt13_GeneticCode.doc
Chpt13_GeneticCode.doc

... These data are from Speyer et al. (1963) Cold Spring Harbor Symposium in Quantitative Biology, 28:559. The theoretical incorporation is the expected value given the genetic code as it was subsequently determined. f. When this mixture of mixed copolymers is used to program in vitro translation, Lys i ...
Lampbrush Chromosomes of the Chicken
Lampbrush Chromosomes of the Chicken

... of meiotic prophase and are characterized by extensive transcription on the loops. An excellent review of LBC investigations and techniques has just been published by Callan (1986). This reference should be consulted for a more detailed discussion. Despite a century of study, we still know relativel ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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