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Constructing DNA
Constructing DNA

... Constructing DNA DNA is called the blueprint of life. It gets this name because it contains the instructions for making every protein in your body. Why are proteins important? Proteins are important because they are what your muscles and tissues are made of; they synthesize the pigments that color y ...
Griffith/Hershey/Chase
Griffith/Hershey/Chase

... to distinguish between them. DNA contains phosphorus and proteins do not; proteins, on the other hand, usually contain sulfur, and DNA does not. By specifically labeling the phosphorus and sulfur atoms with radioisotopes, Hershey and Chase could distinguish unambiguously between the protein and the ...
Abrams Presentation for 11/22 and 11/29
Abrams Presentation for 11/22 and 11/29

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Intorduction to basic vaccine technology - UK-CAB
Intorduction to basic vaccine technology - UK-CAB

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DNA Structure and Replication

... ! H-bonds are weak bonds, broken by high temperature ! DNA “melts”--strands separate--at 85-95oC ! When cool, the strands can re-associate, if they have complementary base sequences ! Use this technique to recognize specific sequences ! make radioactive DNA of particular sequence ! fix unknowns to m ...
Saturday Study Session 2 Theme of the day: Information Transfer
Saturday Study Session 2 Theme of the day: Information Transfer

Biotechnology Lab
Biotechnology Lab

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... Name some proteins • Structural – Keratin ( hair, skin and nails ) Reactions Enzymes, lactase, Signal Insulin, human growth hormone Hemoglobin- carriers oxygen in your red ...
Chapter 12: Genetic Engineering
Chapter 12: Genetic Engineering

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... 7. The genetic code is redundant/degenerate but unambiguous. Clearly explain (a) how the code is redundant and (b) why the code must be unambiguous. The genetic code is redundant because more than one codon can specify a particular amino acid due to (1) wobble and (2) many amino acids are carried by ...
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Ch 11- Controlling Gene Expression

... • Coordinating gene expression- eukaryotes rarely have operons, so enhancer sequences and transcription factors are important for the transcription of genes ...
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Genetic engineering – stepping stones

... Genetic engineering – stepping stones Draw a path across the stepping stones in the correct order for each process. 1) Dolly the sheep – cloning technique ...
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How is a vaccine prepared?

... pollen, insect toxins) that are normally harmless. These people have an allergy. • The immune system reacts by releasing chemicals called histamines, which can cause runny nose, sneezing, rashes and ...
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... 13. inbreeding 14. genetic engineering – 15. restriction enzymes – 16. recombinant DNA – 17. vector – 18. Plasmids 19. Gel Electrophoresis – 20. DNA fingerprint – 21. Transgenic organisms – 22. What are some products produced by genetic engineering? 23. Gene therapy – 24. What medical advances have ...
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Genetic Engineering

... are located on human chromosomes DNA fingerprinting has been used in criminal investigations because- DNA analysis allows investigators to distinguish body cells of different individuals, who are unlikely to have the same DNA ...
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Systematic Implications of DNA variation in subfamily
Systematic Implications of DNA variation in subfamily

... but there are some exceptions Plastid genome – uniparentally (maternally) inherited - rbcL – ribulose-bisphosphate carboxylase large subunit - ndhF – NADH dehydrogenase subunit F - atpB – ATP synthetase subunit B - matK – maturase subunit K - rpl16 intron – ribosomal protein L16 intron Nuclear genom ...
File - Pomp
File - Pomp

... • Neutrophils • 60-70% WBCs; engulf and destroy microbes at infected tissue Short lived • Monocytes • 5% WBCs; develop into…. – Macrophages 1)phagocytosis – 2)lysosomal enzymes destroy microbes ...
Recombinant Expression of PDI in E. coli
Recombinant Expression of PDI in E. coli

... • A process that allows E. coli to be able to uptake the vector containing the foreign DNA • Weaken cell walls. This can be done chemically (CaCl2 solution), or through electroporation. Ours were done chemically. • Heat Shock the cells for 30 seconds so that cells swell • Quick chill to make vectors ...
Innate immunity against malaria: studies on the mechanisms of Plasmodium -phagocyte interactions and their consequences.
Innate immunity against malaria: studies on the mechanisms of Plasmodium -phagocyte interactions and their consequences.

... immunity. The potential for innate immune mechanisms to provide rapid protection against malaria have largely been neglected. Recent studies from animal models, and clinical studies have demonstrated that innate immune cells directed against Plasmodium infected red blood cells contribute to protecti ...
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Science 9 Chapter 4 Practice Test
Science 9 Chapter 4 Practice Test

... c. happens every time an individual produces new cells. d. happens only when a geneticist uses gene therapy. A neutral mutation a. does not affect the organism. b. never happens since all mutations affect an individual. c. cannot be transmitted to the next generation. d. will not be seen until two o ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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