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Basics in Genetics
Basics in Genetics

... Thus most mutations recessive!! Null mutation= makes no protein or totally non-functional protein. Weak or Hypomorphic mutation= makes protein that retains some but not all function. Loss of function mutation vs. Gain of function mutation c. One gene has different alleles. Normal allele = wild type. ...
Immune System - World of Teaching
Immune System - World of Teaching

... Active Helper T-Cell activates Cytotoxic T-Cells and B-Cells. Cytotoxic T-Cells divide into Active Cytotoxic T-cells and Memory T – Cells. Active Cytotoxic T-Cells kill infected cells. At the same time, B-Cells divide into Plasma Cells and Memory B- Cells. Plasma cells produce antibodies that deacti ...
File
File

Chapter 8
Chapter 8

... 8.1 DNA and the Importance of Proteins 1. What is a gene? A gene must be able to make copies of itself; mutate; store information that determines the characteristics of a cell; use this information synthesize proteins. 2. What four functions are performed by nucleic acids? 1) store information that ...
Course: Immunopathology and Immunotherapeutics
Course: Immunopathology and Immunotherapeutics

Biotechnological Tools and Techniques
Biotechnological Tools and Techniques

... • circular pieces of non-chromosomal DNA found in bacteria cells • artificial plasmids have been engineered to contain an area with many recognition sites (and none in other areas) ...
Genetics Online Scavenger Hunt
Genetics Online Scavenger Hunt

...  What is a Gene?  What is a Chromosome?  What is a protein?  What is Heredity?  What is a Trait? 3. As you go from one tutorial to the next answer the corresponding questions for each topic. ...
Innate immune responses to cationic antimicrobial peptides in the lung
Innate immune responses to cationic antimicrobial peptides in the lung

... least partially due to pre-existing inflammation. In addition there is no question that inflammatory response plays an important role in late stage CF lung disease. Cationic antimicrobial peptides have been proposed as a therapeutic option for the treatment of CF. The ability of peptides to kill P. ...
Lab Aseptic Techniques and Classification
Lab Aseptic Techniques and Classification

... The proteins (antigens) are positioned on the filter exactly as they were on the gel. The filter is then washed with patient’s serum followed by anti-human antibodies tagged with an enzyme. The patient antibodies that combine with their specific antigen are visible (shown here in red) when the enzym ...
Bacteria and Recombinant DNA
Bacteria and Recombinant DNA

... The modification of the genotype of a cell (usually prokaryotic) by introducing DNA from another source The uptake of DNA from an organism’s environment The uptake and expression of DNA in a bacterium ...
Manipulating DNA extracting and studying DNA
Manipulating DNA extracting and studying DNA

... for concerns about their safety , and it does seem that foods made from GM plants are safe to eat. (At least that is what the companies marketing the products claim. The other side doesn’t have enough money or the research facilities to undergo such large scale research.) ...
Immunological Defence Mechanisms Against Biological
Immunological Defence Mechanisms Against Biological

... A vaccine can be developed in many ways: killed (denatured) or non replicating pathogens (viruses), recombinant protein antigens, live, attenuated (less harmful) strains of pathogens (for instance cowpox virus to protect against smallpox …) Vaccines can be very effective: cases of smallpox (extincti ...
Human Heredity - mccombsscience
Human Heredity - mccombsscience

... For a long time, reading the DNA sequences in the human genome seemed impossible (the smallest chromosome contains nearly 50 million base pairs!) ...
Lab - Recombinant DNA Simulation
Lab - Recombinant DNA Simulation

... Notice the EcoR1 cut sites are not directly across from each other on the DNA molecule. When EcoR1 makes its cuts, it leaves single-stranded “tails” on the new ends (see above). This type of end is called a STICKY END because complementary DNA sticky ends can be easily rejoined. In this activity, we ...
Day 58 - upwardsapbio
Day 58 - upwardsapbio

File
File

... characteristics in a line of organisms, but increases the risk of genetic defects in certain animal breeds. 17. To produce a fruit that has some characteristics of an orange and some of a grapefruit, you would use the selective breeding technique of ...
Human Mitochondrial DNA
Human Mitochondrial DNA

... more plasmids are “sucked” in. The culture is then quickly transferred to the ice which traps the plasmids within the cell’s membrane. Cells containing the foreign DNA grow and multiply within the tube, but to ensure that transformation was successful and purification of the gene of interest to proc ...
Sample Question Evaluations
Sample Question Evaluations

... c. The body uses it up as energy d. Half out of the body in secretions and half used up as energy 3. A patient has been identified as having a very virulent bacterial infection that is spread through close physical contact. To decrease the chance of spreading this organism, which infection control p ...
35. Modeling Recominant DNA
35. Modeling Recominant DNA

... restriction enzymes found in bacteria. Each particular enzyme recognizes a specific, short, nucleotide sequence in DNA molecules. The restriction enzyme will cut the backbone of the molecules and result in a set of double-stranded DNA fragments with singlestranded “sticky ends.” These ends are refer ...
AP Biology Study Guide
AP Biology Study Guide

... Steamboat Springs High School AP Biology ...
DNA Technology
DNA Technology

...  Vector may have particular genes to aid in recognition of of cell clones  vector - bacterial plasmid  Has ampR – ampicillin resistance gene  Has lacZ gene – catalyzes hydrolysis of lactose sugar – at restriction site, so the enzyme cuts in middle of gene  Gene - human gene of interest ...
Notes on Mutations - Solon City Schools
Notes on Mutations - Solon City Schools

Recombinant DNA Technology
Recombinant DNA Technology

Gene Cloning
Gene Cloning

... – These are often a symmetrical series of four to eight bases on both strands running in opposite directions. – If the restriction site on one strand is 3’-CTTAAG-5’, the complementary strand is 5’-GAATTC-3 ...
AP Biology - gwbiology
AP Biology - gwbiology

... 4. Define the following terms a. Restriction site b. Restriction fragments c. Sticky end 5. Label the following diagram. ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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