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Bioinformatics Research - Purdue University :: Computer Science
Bioinformatics Research - Purdue University :: Computer Science

Supplemental Data
Supplemental Data

... Supplemental Figure S3. Positive colonies of transformants, PCR and RT-PCR analysis of ble-egfp DNA and transcripts in D. bardawil cells electroporated with pZBET. Transformed cells were spread onto Zeocin selective plate immediately (A). When colonies appeared, single colony was transferred to sel ...
Biology 102 Lecture 12: From DNA to Proteins
Biology 102 Lecture 12: From DNA to Proteins

... Genes are further protected with interspersed non--coding regions called introns non ...
The Immune System: The Mind Body Connection
The Immune System: The Mind Body Connection

...  GI tract – to attack food borne pathogens  Respiratory tract – for air borne pathogens ...
The HPV life cycle has implications for the immune response
The HPV life cycle has implications for the immune response

... • Non-regressing papillomas contain few immune cells and no inflammation • Regression is associated with a Th1 type cell mediated immune response and increased expression of IFN-g, IL-12, and TNF-a • There is a large infiltration of mononuclear cells in the epithelium and stroma. This consists of CD ...
Isotype switching Diversification of Igs after B cells encounter antigens
Isotype switching Diversification of Igs after B cells encounter antigens

...  B cell epitope - can bind to an antigen-binding site on an antibody molecule  Linear epitopes – the antibody binds parts of the molecule that are adjacent in the linear sequence (For example a string of amino acids that are in linear sequence in a protein).  Conformational or discontinuous epito ...
Student Handout - University of California, Irvine
Student Handout - University of California, Irvine

... Uses of Gel Electrophoresis:  Gel electrophoresis is used to provide genetic information in a wide range of data fields. Human DNA can be analyzed to provide ________________ in criminal cases, to diagnose _____________ diseases, and to solve _______________ cases. Samples can be obtained from any ...
10.1 filled in notes CD
10.1 filled in notes CD

... As the cell prepares to divide, the chromosomes condense even further ensuring that the extremely long DNA molecules do not get tangled during cell division. ...
Grading rubric DNA Project Unit
Grading rubric DNA Project Unit

... 2. Project DNA chart Requirements: shows 6 codons for DNA and RNA, 6 amino acids, 6 traits 3. DNA transcribed into RNA Requirements: all DNA translated correctly into RNA 6 traits should be visible 4. Colored picture of the person Requirements: features for chin, skin color, ears, nose, Eye color, a ...
Secondary structure of RNA
Secondary structure of RNA

... autoimmune diseases, the body harms its own healthy cells and tissues. This leads to inflammation and damage of various body tissues. Lupus can affect many parts of the body, including the joints, skin, kidneys, heart, lungs, blood vessels, and brain. Although people with the disease may have many d ...
Ethanol precipitation of DNA with salts
Ethanol precipitation of DNA with salts

... 2 fold alcohol to precipitate the DNA. In high conc. of DNA the water DNA intraction is high and “left over” water molecule availability is very low so there is no requirement for NaAce or any other salt. Some time you will need more alcohol to precipitate some nucleotides; for example small mol. RN ...
ppt - Marric.us
ppt - Marric.us

... • The skin serves as a physical barrier to prevent the passage of many disease-causing microorganisms. The skin is also slightly acidic and has good bacteria. ...
CHALLENGES IN CONTROLLING VIRAL DISEASES OF POULTRY
CHALLENGES IN CONTROLLING VIRAL DISEASES OF POULTRY

... bacteria as carriers and DNA plasmids as genetic vaccines. However, very few recombinant vaccines are available commercially. The majority of these newer vaccines are live recombinant viral vectors based on fowlpox virus and herpesvirus designed to deliver specific gene(s) to stimulate the host’ imm ...
Protein Synthesis Review Guide
Protein Synthesis Review Guide

... Mutations are a change in the sequence of nucleotide bases. This can happen during Replication (DNA  DNA) or during Transcription (DNA  RNA). There are 3 major types of mutations: 1) Point Mutation: A mutation that involves a single nucleotide is called a POINT mutation (it happens at a single poi ...
BIOLOGY PRESENTATION
BIOLOGY PRESENTATION

... inheritance with two alleles one D and one small d which doesn’t have the D antigen. So individuals that are DD and Dd are Rh + and individuals that are dd are Rh negative. Rh group incompatibilies can cause serious problems during transfusion but the model that relates to my subject is the mother-f ...
2008 Academic Challenge BIOLOGY TEST
2008 Academic Challenge BIOLOGY TEST

... 32. Which of the following is not true? a. Sickle cell anemia may be described by anemia, poor circulation, or sickle shaped red blood cells. b. Huntington’s disease is characterized by benign tumors that occur under the skin or deeper. c. Cystic fibrosis is characterized by mucus build up in the lu ...
Supplementary Information
Supplementary Information

Kelsie Pombo - Epigenomics and Aging
Kelsie Pombo - Epigenomics and Aging

... maintain healthy homeostatis of cells in tissues, but alterations in their normal expression can lead to cancer. ...
chapter review answers
chapter review answers

... 6. What is an anticodon? What role does it play? An anticodon is a set of complementary bases on transfer RNA that bind to a mRNA to bring the correct amino acid during polypeptide formation. ...
Making recombinant baculovirus
Making recombinant baculovirus

... which can only be rescued upon homologous recombination with the transfer vector). Add 2-5 µg of the recombinant baculovirus transfer vector (e.g. gene of interest cloned into pVL1392/93, orientation must be correct relative to the polyhedrin promoter, some vectors (pFastBac) are not compatible with ...
immunesystem
immunesystem

... • What is the 1st line of defense as shown in the video presentation? Give three explains that were shown. • What is the body’s 2nd line of defense as shown in the video presentation? What is the inflammatory response? • What is an interferon? ...
Chapter 9: Tools for Analyzing Gene Expression
Chapter 9: Tools for Analyzing Gene Expression

... • Constructing fusion proteins with an easy-todetect tag. ...
DNA (Deoxyribonucleic Acid)
DNA (Deoxyribonucleic Acid)

... 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC ...
genetics, dna replication, protein synthesis, biotechnology
genetics, dna replication, protein synthesis, biotechnology

... 16. DNA and RNA molecules are similar in that they both contain a. Nucleotides b. A double helix c. Deoxyribose sugars d. Thymine 17. Which series is arranged in correct order according to decreasing size of structures? a. DNA, nucleus, chromosome, nucleotide, nitrogenous base, b. Nucleotide, chromo ...
RNA
RNA

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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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