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Immunology
Immunology

... ◦ Antigens-a marker on the surface of cell that identifies it as “self” or “non-self” ◦ Antibody-a substance produced by B lymphocytes in response to the presence of a foreign antigen that will combine with and control the antigen, thus preventing infection ◦ Immunity-a long term condition of protec ...
RT-PCR - Faperta UGM
RT-PCR - Faperta UGM

... established protocols ...
Repeated DNA sequences - lecture 1
Repeated DNA sequences - lecture 1

... The alpha 2(1) collagen gene is 38kb long with over 50 exons. Each exon is 54 or 108bp long, i.e. an exact multiple of the 3 amino-acid repeat; 6 or 12 copies. ...
Biology Final Review
Biology Final Review

... bigger cattle that produce more milk c. bacteria to consume pollutants d. all of the above _____29. The replacement of a defective gene with a normal gene is called _____. A. cloning C. plasmid B. genetic engineering D. gene therapy ...
Topic 5: On the Wild Side
Topic 5: On the Wild Side

... grow in the different climatic conditions, therefore analysis of what pollen/spores in the peat gives an idea of what the temperature was like. The lower you go into the peat bog, the longer the time difference. Correlation – When one variable changes, another changes Causation – when one variable c ...
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation

... replication. Can the cell utilize lactose? (i.e. is the operon inducible?) Why or why not? The cell cannot utilize lactose because the promoter is defective. The addition of a normal promoter on a plasmid cannot substitute because promoters act in cis – the promoter must be upstream of the gene it r ...
Aucun titre de diapositive
Aucun titre de diapositive

... *Sub-unit vaccine candidates that provide protection have been identified *Protection conferred by sub-unit vaccine candidates are superior to BCG in a murine model *However, in other animal models no protection superior to BCG or even equal to BCG was observed *Prime boost protocols including BCG, ...
Specific Host Defense IMMUNOLOGY
Specific Host Defense IMMUNOLOGY

... 1. Active: Ab produced the body, long: A. Natural: Infections; protective Ab B. Artificial: Vaccination ...
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... Some antibiotics inhibit cell wall synthesis and others bind to ribosomes to inhibit protein synthesis ...
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DNA Replication - OG

... An insertion mutation is when a nitrogen base is added to the existing DNA A deletion mutation is when a nitrogen base is subtracted from the DNA A substitution mutation is when one nitrogen base is put in place of another. If our DNA was AATTGGCC An insertion would be AATTAGGCC A deletion would be ...
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Chapter 13

... Many egg cells are large enough that DNA can be directly injected into the nucleus Once inside the nucleus, enzymes normally responsible for DNA repair and recombination may help to insert the foreign DNA into the chromosomes of the injected cell. Like bacterial plasmids, the DNA molecules used for ...
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siRNA therapy delivery etc.pptx

... siRNA
Design
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use
of
longer
dsRNA
lead
to
a
non‐specific
 Type
I
interferon
response
(widespread
changes
in
 protein
expressionapoptosis)
 •  Dr.
Thomas
Tuschl’s
lab
discovered
that
RNAi
is
 mediated
by
21
and
22
nt
RNAs
 •  Also
discovered
the
important
characteristics
needed
 by
the
R ...
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Biotechnology

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Mock Exam 3 Chapters 14-18 Anthony Todd http

... c. MIH which inhibits the formation of Mullerian ducts so male development can continue d. A and B are correct e. B and C are correct Use the following information for Questions 15 and 16: A dominant sex-linked gene B produces white bars on black chickens. A clutch of chickens has equal numbers of b ...
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... for 1 hour in Assay Buffer in the presence of CFX. 0.2 % SDS and 0.1 mg/ml Proteinase K are added before a further incubation at 37 C for 30 minutes. gyrase ...
CP Biology 9.2 Copying DNA PCR uses polymerase to copy DNA
CP Biology 9.2 Copying DNA PCR uses polymerase to copy DNA

... DNA might be used to make a DNA fingerprint. The more regions that are used, the less likely it is that two people will have the same DNA fingerprint. There is a very small change – in in many million – that two people have the same DNA fingerprint. DNA fingerprinting is used for many different purp ...
Chapter 2 Antigen
Chapter 2 Antigen

... antigens are taken into the antigenpresenting cells (APCs) and processed into fragments. ...
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... had human genes added to their DNA so that human proteins are secreted into the whites of their eggs, along with complex medicinal proteins similar to drugs used to treat skin cancer and other diseases. ...
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Biochemistry of the immune system

... • Interleukins - presumed targets are principally leukocytes. • Lymphokines - produced by activated T lymphocytes direct the immune system response by signaling between its cells ...
Synthetic Nanovaccines
Synthetic Nanovaccines

... in mice also are present in humans with Parkinson’s disease. The cause of Parkinson’s disease — which affects more than 4 million people worldwide — is the loss of neurons that produce dopamine, a nerve signaling chemical that controls movement and balance. Neurodegeneration occurs when a normal pro ...
Protocol for Phage T1-Resistant TransforMax™ EC100™
Protocol for Phage T1-Resistant TransforMax™ EC100™

... Note: Refrozen cells may have reduced transformation efficiency. 6. Transfer the desired amount of DNA and 50 μl of cells to a pre-chilled microcentrifuge tube. Note: A smaller volume of cells can be used based on the needs and experiences of the user. Mix the cells and DNA by pipetting up and do ...
in this issue - The Journal of Immunology
in this issue - The Journal of Immunology

... that is used as a model for human systemic lupus erythematosus. NZB/W mice exhibit an abnormal expansion of an autoreactive B1a cell population in both the peritoneal cavity and spleen. Balababian et al. (p. 3392) studied the effect of stromal cell-derived factor-1 (SDF-1/CXCL12) on peritoneal B1a c ...
Biotechnology
Biotechnology

... world to map the genes of a human. B. The purpose of the HGP was to identify the location of genes on specific chromosomes to better understand human genetics. C. A primary application of the Human Genome Project is to determine whether individuals may carry genes for genetic conditions such as sick ...
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... C. allows crossing over during meiosis. D. removes exons from an RNA molecule. E. occurs in the cytosol. 8. The enhancers located near the albumin gene A. are only present in liver cells. B. bind transcription factors only found in the liver. C. are located in introns. D. change the position at whic ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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