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Green, Ed, NEADERTHAL DNA, UC Santa Cruz, June
Green, Ed, NEADERTHAL DNA, UC Santa Cruz, June

... one set of dna from mom and one set from dad... that is what DIPLOID means. where in that genome are these uniquely human characteristictics encoded... what makes humans unique genetically?. 35M SNP changes (single nucleotide polymorphisms.) 5M insertion/deletions 9 pericentric inversions and 1 chro ...
Chapter 24
Chapter 24

... - Innate defenses include the skin and mucous membranes, phagocytic cells, and anti-microbial proteins. - The inflammation mobilizes nonspecific defense forces. - The lymphatic system is a crucial system during infection. - Antigens have specific regions where antibodies can bind. - Helper T cells h ...
Prodution of Biopharmaceuticals : An Overview
Prodution of Biopharmaceuticals : An Overview

... particular virus strains by cultivating them on cells other than target cells, in which the virus infection followed by repeated passaging will be performed The cell-culture process for vaccines offers high potential as an alternative method to egg-based production. Cell culture has the capability t ...
Gel Electrophoresis of DNA
Gel Electrophoresis of DNA

... gel is run. The time of the run depends upon the amount of current and % gel, and requires experimentation • At the end of the run the gel is removed (it is actually quite stiff) • The gel is then visualized - UV light causes the bands of DNA to fluoresce ...
Ch21A - MDC Faculty Home Pages
Ch21A - MDC Faculty Home Pages

... ______________________________________, these cells become activated. T cells mature in the thymus under negative and positive selection pressures. _________________________ Selection selects T cells capable of binding to _______________________ proteins, and ______________ attack. _________________ ...
The discovery of the structure and function of the genetic substance
The discovery of the structure and function of the genetic substance

... 1869 – Discovery of DNA by Miescher 1909 – Chemical structure of nucleotide shown by Levene 1928 – Deoxyribose discovered by Levene 1939 – First X-ray diffraction of DNA by Astbury 1944 – Genetic function of DNA proven by Avery 1949 – X-ray structure of nucleoside by Furberg 1950 – a-helix shown in ...
Biology Final Review
Biology Final Review

DNA Replication - Living Environment H: 8(A,C)
DNA Replication - Living Environment H: 8(A,C)

... • Occurs inside the nucleus of the cell • Occurs when the cell is going to divide so each resulting cell will have a complete set of DNA • During DNA replication, the DNA separates into two strands, then produces ...
Lymphocyte activity and protein synthesis
Lymphocyte activity and protein synthesis

... understanding of what lymphocytes do derives from studies ex vivo or in vitro. Of course, lymphocytes do many things. They proliferate in a clonal fashion in response to antigen. They probably also divide without specific stimulation, or possibly as a consequence of so-called bystander activation, i ...
APUnit9sheet2017
APUnit9sheet2017

... 5. (Based on HW and class work) Relate HIV life cycle to the function of the immune system to explain why AIDS is a particularly devastating disease. 5. What is a vaccine? 6. What are factors common to emerging viruses and the types of viruses that can cause epidemics? 7. What is the difference betw ...
Transduction
Transduction

... repressed. In many cases the prophage is inserted into the bacterial DNA and replicates as part of the chromosome. When lysogeny breaks down and the phage enters the lytic cycle, it is excised from the chromosome by recombination between sequences at each end of the integrated prophage. If this reco ...
SBI 3C genetics Study Guide (SPRING 2015)
SBI 3C genetics Study Guide (SPRING 2015)

... Describe the 3 reasons why cells need to divide Describe the phases of the cell cycle (including mitosis and cytokinesis and the 3 phases of interphase) What is asexual reproduction? Provide examples of organisms that divide through asexual reproduction and compare the DNA in the parent to the DNA i ...
The Hereditary Material - Advanced
The Hereditary Material - Advanced

... from S strain bacteria and tested the remaining compounds for the ability to cause transformation. If the remaining material did not cause transformation, than that material could not be the heredity material. Avery and his colleagues treated the S strain bacteria with the protease enzymes trypsin a ...
Memorandum of Understanding and Agreement (MUA)
Memorandum of Understanding and Agreement (MUA)

... appropriate NIH Guidelines and/or USDA/APHIS, and EPA regulations. Please include the following (if applicable): a. Source or type of Infectious Agent (List complete Latin names for organisms, no abbreviations) and host (i.e. cell line, animal species); b. Recombinant DNA/Plasmids/Vectors to be used ...
basicprinciplesofimmunesystem
basicprinciplesofimmunesystem

... - create immune memory  provide more effective response in further secondary infection  basic principle of vaccination. - create focus response to certain/fixed antigen that invades body & eliminate/destroy it  increase protective capacity of innate immunity. ...
The Basics of Cancer Biology
The Basics of Cancer Biology

... regimens were compared • The primary endpoint was Pathological Complete Remission (absence of tumor cells by histology) which predicts survival • Tumors with higher content of T-cells (or T-cell derived transcripts) responded much better in terms of pCR • These results suggest that the most importan ...
Gene Section CDKN1A (cyclin-dependent kinase inhibitor 1A) Atlas of Genetics and Cytogenetics
Gene Section CDKN1A (cyclin-dependent kinase inhibitor 1A) Atlas of Genetics and Cytogenetics

... CDKN1A is implicated in regulation of cell growth and cell response to DNA damage. It inhibits cell cycle progression in G1 by binding to G1 cyclin-CDK complexes and to PCNA antigen and may also induce G2 arrest. In response to DNA damage p53 induces CDKN1A expression, which is responsible for the c ...
supplementary materials
supplementary materials

... Mutagenesis of the MSE at SPO77. The promoter region of SPO77 was amplified by PCR from -710 to -1 nucleotides relative to the translation start site. The 5’ oligo is located 150 nucleotides in the RPP0 locus, an essential gene encoding a cytoplasmic component of the ribosome [6]. The 710 base-pair ...
MLPA Assay using GSP Kit
MLPA Assay using GSP Kit

... MLPA (Multiplex ligation-dependent probe amplification) is a DNA-based technique developed by Schouten et al., for the detection of duplications and deletions of whole genes and individual exons. It is now widely used in both research and diagnostic genetics laboratories with a large number of comme ...
The Immune System
The Immune System

... body against a wide variety of infections. They are divided into two groups, depending on where they mature : i) B lymphocytes (B cells) mature in bone marrow and  ii) T lymphocytes (T cells) mature in the thymus gland. ...
Acute Phase Proteins and other Systemic
Acute Phase Proteins and other Systemic

... If there are insufficient protein reserves there is: ...
Bacterial Nucleic Acids
Bacterial Nucleic Acids

... • Their information is used to make protein with the help of RNA through Transcription...Translation. • The DNA double helix is stabilized by hydrogen bonds between the bases attached to the two strands. • One major difference between DNA and RNA is the sugar, with the 2deoxyribose in DNA being repl ...
Med Sch lecture Immunology Laboratory SB 2012
Med Sch lecture Immunology Laboratory SB 2012

The Lymphatic System
The Lymphatic System

... receptors for the self-antigens just like for any other antigens, but that those cells are killed off before they reach maturity. If those cells did reach maturity and enter the blood stream, our lymphatic system would attack some of our own cells. It is believed this is how multiple sclerosis works ...
Thermo Scientific Top Vision Low Melting Point Agarose
Thermo Scientific Top Vision Low Melting Point Agarose

... granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of the product does not include or carry any right or license to use, develop, or otherwise exploit this product commercially and no rights are conveyed to the buyer to use the product or co ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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