Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was a shift (albeit it was small) to shorter wavelengths of the emission spectrum. This means that Hoechst not on ...
... with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was a shift (albeit it was small) to shorter wavelengths of the emission spectrum. This means that Hoechst not on ...
In Vitro Cell. Dev. Biol. In Press
... resistant to disease is essential in breeding programs because agricultural yields are reduced constantly by various diseases caused by phytopathogenic fungi, bacteria, and viruses (Tabei et al., 1998). Breeding for disease resistance has become one of the most crucial objectives in cucumber cultiva ...
... resistant to disease is essential in breeding programs because agricultural yields are reduced constantly by various diseases caused by phytopathogenic fungi, bacteria, and viruses (Tabei et al., 1998). Breeding for disease resistance has become one of the most crucial objectives in cucumber cultiva ...
Divergent TLR7 and TLR9 signaling and type I interferon production
... cells15,17 and facilitating the induction of antiviral CD8+ T cell, CD4+ T cell, NK cell and B cell responses18–20. In both sooty mangabeys and rhesus macaques, DCs could be divided into pDCs and mDCs with antibodies for CD123 and CD11c, respectively (Fig. 1d). As neither pDC nor mDC populations hav ...
... cells15,17 and facilitating the induction of antiviral CD8+ T cell, CD4+ T cell, NK cell and B cell responses18–20. In both sooty mangabeys and rhesus macaques, DCs could be divided into pDCs and mDCs with antibodies for CD123 and CD11c, respectively (Fig. 1d). As neither pDC nor mDC populations hav ...
Journal of Biotechnology Rapid and highly efficient mammalian cell
... protein/gRNA RNP complexes were delivered into cultured human fibroblasts and induced pluripotent stem cells (iPSC) via electroporation with high efficiency and relatively low off-target effects (Kim et al., 2014). In that study, a large amount of Cas9 protein (4.5–45 g) and gRNA (6–60 g) were neces ...
... protein/gRNA RNP complexes were delivered into cultured human fibroblasts and induced pluripotent stem cells (iPSC) via electroporation with high efficiency and relatively low off-target effects (Kim et al., 2014). In that study, a large amount of Cas9 protein (4.5–45 g) and gRNA (6–60 g) were neces ...
CD8 T cell activation predominate early immune
... Abstract Background: It is well established that adaptive immune responses induced by hypercholesterolemia play an important role in the development of atherosclerosis, but the pathways involved remain to be fully characterized. In the present study we assessed immune responses to hypercholesterolem ...
... Abstract Background: It is well established that adaptive immune responses induced by hypercholesterolemia play an important role in the development of atherosclerosis, but the pathways involved remain to be fully characterized. In the present study we assessed immune responses to hypercholesterolem ...
Charge Transport in DNA - Insights from
... DNA molecules between the electrodes were breaking. The fundamental features of CT in DNA have been described as well. First, the exponential dependence of CT efficiency on the length of the DNA oligomers. Second, the role of experimental setup that is both crucial and challenging to characterize. In ...
... DNA molecules between the electrodes were breaking. The fundamental features of CT in DNA have been described as well. First, the exponential dependence of CT efficiency on the length of the DNA oligomers. Second, the role of experimental setup that is both crucial and challenging to characterize. In ...
Equilibrium Statistics of Channel-confined DNA
... and visualising single DNA molecules have been developed by biologists. Similarly, the development of clean room patterning techniques allows for the manufacturing of well-defined geometries down to the nanometre scale [2, 5, 17]. This has enabled the testing of old polymer theory predictions [18–23 ...
... and visualising single DNA molecules have been developed by biologists. Similarly, the development of clean room patterning techniques allows for the manufacturing of well-defined geometries down to the nanometre scale [2, 5, 17]. This has enabled the testing of old polymer theory predictions [18–23 ...
Part III: Laboratory – Electrophoresis
... approximately an eighth of an inch in diameter. If the leaves are too small, take tissue from multiple leaves (from the same plant) until you have the equivalent amount of leaf tissue. Note: Plants with the ago-1 phenotype are so small that you may have to use the entire plant. If you use the entire ...
... approximately an eighth of an inch in diameter. If the leaves are too small, take tissue from multiple leaves (from the same plant) until you have the equivalent amount of leaf tissue. Note: Plants with the ago-1 phenotype are so small that you may have to use the entire plant. If you use the entire ...
Department of Immunology, the Wenner-Gren Institute Stockholm University, Stockholm, Sweden Doctoral thesis
... Fulani, are not due to a general hyper-responsiveness in this group, but neither a malaria specific response. Rather, the higher responses in the Fulani are pathogen related, certain antigens/pathogens being more immunogenic in the Fulani ethnic group. Moreover, it appears as if the IgG subclass pat ...
... Fulani, are not due to a general hyper-responsiveness in this group, but neither a malaria specific response. Rather, the higher responses in the Fulani are pathogen related, certain antigens/pathogens being more immunogenic in the Fulani ethnic group. Moreover, it appears as if the IgG subclass pat ...
Population dynamics of immune repertoires
... The general idea behind this class of models is that one important signal for which lymphocytes compete comes from antigens. Strong antigenic recognition by mature lymphocytes generally triggers clonal expansion into effector and memory cells. These strong signals are usually of pathogenic origin wi ...
... The general idea behind this class of models is that one important signal for which lymphocytes compete comes from antigens. Strong antigenic recognition by mature lymphocytes generally triggers clonal expansion into effector and memory cells. These strong signals are usually of pathogenic origin wi ...
localization of histone gene transcripts in newt lampbrush
... Ideal preparations have the bivalent chromosomes well separated from one another, all their lateral loops should be anchored down on the coverglass surface, and they should be reasonably free from nucleoli. These considerations determine the ideal oocyte size with which to work, as large as possible ...
... Ideal preparations have the bivalent chromosomes well separated from one another, all their lateral loops should be anchored down on the coverglass surface, and they should be reasonably free from nucleoli. These considerations determine the ideal oocyte size with which to work, as large as possible ...
Pathogen Response Genes Mediate Caenorhabditis elegans Innate
... innate immune response in C. elegans was originally described as a non-specific, first line of defense against infection, serving to detect pathogens and initiate a neutralizing response (Shivers et al. 2008; Akira et al. 2006). However, recent studies suggest a greater specificity than was initial ...
... innate immune response in C. elegans was originally described as a non-specific, first line of defense against infection, serving to detect pathogens and initiate a neutralizing response (Shivers et al. 2008; Akira et al. 2006). However, recent studies suggest a greater specificity than was initial ...
![Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides](http://s1.studyres.com/store/data/023790436_1-6c83654c03a0a35fc50303cc056eabf6-300x300.png)
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... Complex between 1 and d(CCGGTACCGG) This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in ...
... Complex between 1 and d(CCGGTACCGG) This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in ...
Site-Directed Mutagenesis Using Oligonucleotide
... an additional suppressor mutation, sbc (suppressor of recB and recC), possess activation of the RecET recombination pathway (sbcA) or enhanced recombination by the RecF pathway (sbcB). recE and recT are found on the defective lambdoid E. coli prophage Rac and encode an exonuclease and a ssDNA-bindin ...
... an additional suppressor mutation, sbc (suppressor of recB and recC), possess activation of the RecET recombination pathway (sbcA) or enhanced recombination by the RecF pathway (sbcB). recE and recT are found on the defective lambdoid E. coli prophage Rac and encode an exonuclease and a ssDNA-bindin ...
Virology: Research and Treatment
... activator, Tax, which include deregulating mitotic checkpoints, inducing nuclear factor (NF)-κB activation, and inactivating tumor suppressors, play a critical role in the early stages of leukemogenesis.5,6 However, Tax expression may require strict regulation during clinical latency and may be expe ...
... activator, Tax, which include deregulating mitotic checkpoints, inducing nuclear factor (NF)-κB activation, and inactivating tumor suppressors, play a critical role in the early stages of leukemogenesis.5,6 However, Tax expression may require strict regulation during clinical latency and may be expe ...
PPT - Altogen Biosystems
... 3. Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
... 3. Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
REVIEWS - Max-Planck-Institut für Biochemie
... mycobacterial survival9. So mycobacteria can survive and replicate intracellularly through other strategies that protect them from subsequent attack by antimicrobial components in the phagosomal maturation pathway. Phagosomal maturation involves a series of sequential fusion events with various vesi ...
... mycobacterial survival9. So mycobacteria can survive and replicate intracellularly through other strategies that protect them from subsequent attack by antimicrobial components in the phagosomal maturation pathway. Phagosomal maturation involves a series of sequential fusion events with various vesi ...
HTLV-1, Immune Response and Autoimmunity
... 1. Introduction The etiology of autoimmune diseases is unknown, but it is clear that the interaction between genes and the environment is an important step in breaking immune tolerance to self-antigens. This can lead to inflammation and destruction of specific tissues and organs. Although host genet ...
... 1. Introduction The etiology of autoimmune diseases is unknown, but it is clear that the interaction between genes and the environment is an important step in breaking immune tolerance to self-antigens. This can lead to inflammation and destruction of specific tissues and organs. Although host genet ...
The role of lymph node sinus macrophages in host defense
... shown to directly target B cells to enhance humoral immune responses upon subsequent antigen challenge.46 These off-target effects should be taken into account when interpreting experimental results that make use of bisphosphonate-based preparations to study macrophage function. An alternative appro ...
... shown to directly target B cells to enhance humoral immune responses upon subsequent antigen challenge.46 These off-target effects should be taken into account when interpreting experimental results that make use of bisphosphonate-based preparations to study macrophage function. An alternative appro ...
Analysis of clones carrying repeated DNA sequences in two YAC
... First, a set of overlapping cosmid clones has been assembled into 750 contigs covering 90-95% of the genome (Hauge et a/., 1991). Second, efforts are underway to construct overlapping YAC contigs for the five Arabidopsis chromosomes. The three YAC libraries commonly in use are the EG (Grill and Some ...
... First, a set of overlapping cosmid clones has been assembled into 750 contigs covering 90-95% of the genome (Hauge et a/., 1991). Second, efforts are underway to construct overlapping YAC contigs for the five Arabidopsis chromosomes. The three YAC libraries commonly in use are the EG (Grill and Some ...
BCelsa_WDA
... specificity and affinity which is characteristic of the parent immunoglobulin. Aside from the CDRs, the Fv also contains more highly conserved framework segments which connect the CDRs and are mainly involved in supporting the CDR loop conformations, although in some cases, framework residues also c ...
... specificity and affinity which is characteristic of the parent immunoglobulin. Aside from the CDRs, the Fv also contains more highly conserved framework segments which connect the CDRs and are mainly involved in supporting the CDR loop conformations, although in some cases, framework residues also c ...
Template for PowerPoint Use
... specificity and affinity which is characteristic of the parent immunoglobulin. Aside from the CDRs, the Fv also contains more highly conserved framework segments which connect the CDRs and are mainly involved in supporting the CDR loop conformations, although in some cases, framework residues also c ...
... specificity and affinity which is characteristic of the parent immunoglobulin. Aside from the CDRs, the Fv also contains more highly conserved framework segments which connect the CDRs and are mainly involved in supporting the CDR loop conformations, although in some cases, framework residues also c ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.