 
									
								
									12-Transcription-The Relationship Between Genes and Proteins
									
... These two processes are closely coupled in prokaryotes, whereas they are spacially and temporally separate in eukaryotes. (A) In prokaryotes, the primary transcript serves as mRNA and is used immediately as the template for protein synthesis. (B) In eukaryotes, mRNA precursors are processed and spli ...
                        	... These two processes are closely coupled in prokaryotes, whereas they are spacially and temporally separate in eukaryotes. (A) In prokaryotes, the primary transcript serves as mRNA and is used immediately as the template for protein synthesis. (B) In eukaryotes, mRNA precursors are processed and spli ...
									Mitochondrial DNA
									
... Both strands are transcribed as single RNA molecules The D loop contains one promoter for each strand, and the entire strand is transcribed. ...
                        	... Both strands are transcribed as single RNA molecules The D loop contains one promoter for each strand, and the entire strand is transcribed. ...
									The Cell in Motion
									
... Call out the fourth role, “Transfer RNA’s come stand in the cytoplasm.” Transfer RNA (tRNA) [binds to the messenger RNA (mRNA) at one end and the amino acid at the other end] (1) Students find the play dough representing their amino acid. (2) Students roll the play dough into small balls to represen ...
                        	... Call out the fourth role, “Transfer RNA’s come stand in the cytoplasm.” Transfer RNA (tRNA) [binds to the messenger RNA (mRNA) at one end and the amino acid at the other end] (1) Students find the play dough representing their amino acid. (2) Students roll the play dough into small balls to represen ...
									presentation (spanish ppt format, 4.7 MB)
									
... Previously known as co-suppression, post transcriptional gene silencing (PTGS) and quelling. In 2006, Andrew Fire and Craig C. Mello shared the Nobel Prize in Physiology or Medicine for their work on RNAi in the nematode worm C. elegans. Two types of RNA molecules involved: - microRNA (miRNA) - smal ...
                        	... Previously known as co-suppression, post transcriptional gene silencing (PTGS) and quelling. In 2006, Andrew Fire and Craig C. Mello shared the Nobel Prize in Physiology or Medicine for their work on RNAi in the nematode worm C. elegans. Two types of RNA molecules involved: - microRNA (miRNA) - smal ...
									QPCR Helpful Hints
									
... UV spectrophotometry and fluorescence (Picogreen for DNA or Ribogreen for RNA). The Nelson Lab typically uses the Nanodrop UV spectrophotometer which only requires 2 l of sample. Whatever method is used (UV or fluorescence) it is important to be consistent and not switch back and forth between quan ...
                        	... UV spectrophotometry and fluorescence (Picogreen for DNA or Ribogreen for RNA). The Nelson Lab typically uses the Nanodrop UV spectrophotometer which only requires 2 l of sample. Whatever method is used (UV or fluorescence) it is important to be consistent and not switch back and forth between quan ...
									Aliens? - Johns Hopkins Bloomberg School of Public Health
									
... – Problem: each element is at least in part unique, and RepeatMasker will mask that too ...
                        	... – Problem: each element is at least in part unique, and RepeatMasker will mask that too ...
									Regulation of Gene Expression
									
... “Consider an individual E. coli cell living in the erratic environment of the human colon, dependent for its nutrients on the whimsical eating habits of the host.” “If the environment is lacking the amino acid tryptophan (which the bacterium needs to survive), the cell responds but activating a ...
                        	... “Consider an individual E. coli cell living in the erratic environment of the human colon, dependent for its nutrients on the whimsical eating habits of the host.” “If the environment is lacking the amino acid tryptophan (which the bacterium needs to survive), the cell responds but activating a ...
									2.4 How DNA Codes for Protein
									
... known as the poly(A) tail or polyadenylation. A second RNA strand known as the functional transcript is made by splicing exons together between the G-cap and the poly(A) tail. Occasionally some exons are omitted during splicing and an alternative protein is coded in the functional transcript. The fu ...
                        	... known as the poly(A) tail or polyadenylation. A second RNA strand known as the functional transcript is made by splicing exons together between the G-cap and the poly(A) tail. Occasionally some exons are omitted during splicing and an alternative protein is coded in the functional transcript. The fu ...
									HW Answers pg. 241,2..
									
... • be transcribed and binds to this site. Binding to the promoter region results in DNA’s double helix opening up. Once • bound to the DNA template strand, RNA polymerase starts to build the mRNA complementary strand using • ribonucleotides. Finally, RNA polymerase recognizes the termination sequence ...
                        	... • be transcribed and binds to this site. Binding to the promoter region results in DNA’s double helix opening up. Once • bound to the DNA template strand, RNA polymerase starts to build the mRNA complementary strand using • ribonucleotides. Finally, RNA polymerase recognizes the termination sequence ...
									video slide - Greensburg
									
... • In prokaryotes, mRNA produced by transcription is immediately translated without more processing • In a eukaryotic cell, the nuclear envelope separates transcription from translation • Eukaryotic RNA transcripts are modified through RNA processing to yield finished mRNA • Cells are governed by a ...
                        	... • In prokaryotes, mRNA produced by transcription is immediately translated without more processing • In a eukaryotic cell, the nuclear envelope separates transcription from translation • Eukaryotic RNA transcripts are modified through RNA processing to yield finished mRNA • Cells are governed by a ...
									Transcription and Translation
									
... exists. • There are a few prokaryotic examples, but most introns are found in eukaryotes. • Some genes have many long introns: the dystrophin gene (mutants cause muscular dystrophy) has more than 70 introns that make up more than 99% of the gene’s sequence. However, not all eukaryotic genes have int ...
                        	... exists. • There are a few prokaryotic examples, but most introns are found in eukaryotes. • Some genes have many long introns: the dystrophin gene (mutants cause muscular dystrophy) has more than 70 introns that make up more than 99% of the gene’s sequence. However, not all eukaryotic genes have int ...
									Let` review answers as a class.
									
... Roger has short ears. Genevieve is heterozygous for long ears. Use the steps below to solve the problem. Determine the parent’s genotype and phenotype ...
                        	... Roger has short ears. Genevieve is heterozygous for long ears. Use the steps below to solve the problem. Determine the parent’s genotype and phenotype ...
									No Slide Title
									
... mRNA, tRNA, rRNA 1. Messenger RNA (mRNA) [~copy of DNA] 2. transfer RNA (tRNA) [binds to amino acid and codon for mRNA] 3 bases correspond to unique amino acid. ...
                        	... mRNA, tRNA, rRNA 1. Messenger RNA (mRNA) [~copy of DNA] 2. transfer RNA (tRNA) [binds to amino acid and codon for mRNA] 3 bases correspond to unique amino acid. ...
									Promoters - Pennsylvania State University
									
... at the start site for transcription. – beta-galactosidase: measure the hydrolysis of an analog of lactose that generates a colored, fluorescent or chemiluminescent product ...
                        	... at the start site for transcription. – beta-galactosidase: measure the hydrolysis of an analog of lactose that generates a colored, fluorescent or chemiluminescent product ...
									Promega Notes: Technically Speaking: Tips for Working with RNA
									
... phenol:chloroform:isoamyl alcohol, adequate extraction reagents for the amount and type of tissue used and proper pH for the extraction reagents. Commercial reagents are preferred because of the difficulty in making adequately pure and consistent reagents at the exact pH necessary to partition DNA ...
                        	... phenol:chloroform:isoamyl alcohol, adequate extraction reagents for the amount and type of tissue used and proper pH for the extraction reagents. Commercial reagents are preferred because of the difficulty in making adequately pure and consistent reagents at the exact pH necessary to partition DNA ...
									BIOL 112 – Principles of Zoology
									
...  In eukaryotes, one mRNA = one protein. (in bacteria, one mRNA can be polycistronic, or code for several proteins).  DNA in eukaryotes forms a stable, compacted complex with histones. In bacteria, the chromatin is not in a permanently condensed state.  Eukaryotic DNA contains large regions of rep ...
                        	...  In eukaryotes, one mRNA = one protein. (in bacteria, one mRNA can be polycistronic, or code for several proteins).  DNA in eukaryotes forms a stable, compacted complex with histones. In bacteria, the chromatin is not in a permanently condensed state.  Eukaryotic DNA contains large regions of rep ...
									1 - El Camino College
									
... 110. Which does NOT describe a function of the DNA polymerase molecule? A.recognize the free nucleotide that pairs with the base on the old strand of DNA B.read the strand of old DNA and recognize the base there C.proofread to ensure that the proper base has been incorporated D.make the proper nucle ...
                        	... 110. Which does NOT describe a function of the DNA polymerase molecule? A.recognize the free nucleotide that pairs with the base on the old strand of DNA B.read the strand of old DNA and recognize the base there C.proofread to ensure that the proper base has been incorporated D.make the proper nucle ...
									AGO1-IP approach to small RNA target discovery in Arabidopsis
									
... A drawback of the method is its intrinsic reliance on sufficient VSR expression levels in the tissues of interest. This may partly explain its poor performances in roots (Figure S5), where the 35S promoter is reputed to be only weakly active in several cell layers. Constitutive VSR expression might ...
                        	... A drawback of the method is its intrinsic reliance on sufficient VSR expression levels in the tissues of interest. This may partly explain its poor performances in roots (Figure S5), where the 35S promoter is reputed to be only weakly active in several cell layers. Constitutive VSR expression might ...
									Exam Review 2 - Fullfrontalanatomy.com
									
... D) enveloped viruses E) bacteriophages 79) At one point, you were just an undifferentiated, single cell. You are now made of many cells; some of these cells function as liver cells, some as muscle cells, some as red blood cells, while others play different roles. What name is given to the process th ...
                        	... D) enveloped viruses E) bacteriophages 79) At one point, you were just an undifferentiated, single cell. You are now made of many cells; some of these cells function as liver cells, some as muscle cells, some as red blood cells, while others play different roles. What name is given to the process th ...
									Final Exam - brownscience
									
... 2. How would the complementary strand of DNA appear if the original strand of DNA contained the bases T-A-GC in that order? 3. DNA or Deoxyribonucleic acid is 4. Which base is normally used in the synthesis of RNA but not in the synthesis of DNA 5. A strand of messenger RNA is transcribed from an or ...
                        	... 2. How would the complementary strand of DNA appear if the original strand of DNA contained the bases T-A-GC in that order? 3. DNA or Deoxyribonucleic acid is 4. Which base is normally used in the synthesis of RNA but not in the synthesis of DNA 5. A strand of messenger RNA is transcribed from an or ...
									An RNA-binding domain in the viral haemorrhagic septicaemia virus
									
... The specificity of this interaction requires the presence of the phosphoprotein. In the absence of this protein, the nucleoprotein is able to bind any RNA (Masters & Banerjee, 1988). In this study, we showed that a short region on VHSV N is implicated in RNA binding, even in the absence of the M1 ph ...
                        	... The specificity of this interaction requires the presence of the phosphoprotein. In the absence of this protein, the nucleoprotein is able to bind any RNA (Masters & Banerjee, 1988). In this study, we showed that a short region on VHSV N is implicated in RNA binding, even in the absence of the M1 ph ...
									Transcription and Translation
									
... exists. • There are a few prokaryotic examples, but most introns are found in eukaryotes. • Some genes have many long introns: the dystrophin gene (mutants cause muscular dystrophy) has more than 70 introns that make up more than 99% of the gene’s sequence. However, not all eukaryotic genes have int ...
                        	... exists. • There are a few prokaryotic examples, but most introns are found in eukaryotes. • Some genes have many long introns: the dystrophin gene (mutants cause muscular dystrophy) has more than 70 introns that make up more than 99% of the gene’s sequence. However, not all eukaryotic genes have int ...
									A Biology Primer for Computer Scientists
									
... DNA replication is the process by which a double-stranded DNA sequence produces two double-stranded sequences identical (in the absence of errors!) to the original one. The way this happens is that the original complementary strands unwind and for each of them a new complementary strand is synthesiz ...
                        	... DNA replication is the process by which a double-stranded DNA sequence produces two double-stranded sequences identical (in the absence of errors!) to the original one. The way this happens is that the original complementary strands unwind and for each of them a new complementary strand is synthesiz ...
RNA
 
                        Ribonucleic acid (RNA) is a polymeric molecule implicated in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the three major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, U, A, and C to denote the nitrogenous bases guanine, uracil, adenine, and cytosine) that directs synthesis of specific proteins. Many viruses encode their genetic information using an RNA genome.Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals. One of these active processes is protein synthesis, a universal function whereby mRNA molecules direct the assembly of proteins on ribosomes. This process uses transfer RNA (tRNA) molecules to deliver amino acids to the ribosome, where ribosomal RNA (rRNA) links amino acids together to form proteins.
 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									