Restriction fragment differential display of pediocin
... fragments. The analysis was therefore carried out with primers degenerate in the third position, NNA\G or NNC\T, giving a 50 % workload reduction. The amplifications contained on average 50–70 fragments (between 10 and 100 fragments), the majority of which were under 400 bp, the largest being 770 bp ...
... fragments. The analysis was therefore carried out with primers degenerate in the third position, NNA\G or NNC\T, giving a 50 % workload reduction. The amplifications contained on average 50–70 fragments (between 10 and 100 fragments), the majority of which were under 400 bp, the largest being 770 bp ...
Supplemental Table 1 and Figure Legends
... Panel A. Pair plots showing correlations between selected samples: Gene expression intensities obtained from samples containing 100 ng of RNA extracted from MDA-MB-468 cells considered as benchmarks and correlated with intensities obtained in profiles using decreasing RNA inputs, respectively 10 ng, ...
... Panel A. Pair plots showing correlations between selected samples: Gene expression intensities obtained from samples containing 100 ng of RNA extracted from MDA-MB-468 cells considered as benchmarks and correlated with intensities obtained in profiles using decreasing RNA inputs, respectively 10 ng, ...
488KB - The Doudna Lab - University of California, Berkeley
... altered end-recognition specificity and RNA product length. These results explain how Dicer functions as a molecular ruler and provide a structural basis for modifying its activity in cells. ...
... altered end-recognition specificity and RNA product length. These results explain how Dicer functions as a molecular ruler and provide a structural basis for modifying its activity in cells. ...
Recognition of Nucleic Acid Bases and Base
... precisely positioned side-chains in the DNA major groove could discriminate amongst all the basepairs: one in which the guanidinium group of Arg donates two hydrogen bonds to the O6 and N7 acceptor groups of guanine and a second in which the carboxamide group of Asn (or Gln) hydrogen bonds to the N7 ...
... precisely positioned side-chains in the DNA major groove could discriminate amongst all the basepairs: one in which the guanidinium group of Arg donates two hydrogen bonds to the O6 and N7 acceptor groups of guanine and a second in which the carboxamide group of Asn (or Gln) hydrogen bonds to the N7 ...
as a PDF
... The B. subtilis trpEDCFBA operon contains six of the seven genes that are required for the biosynthesis of tryptophan from chorismic acid, the common aromatic amino acid precursor (Fig. 1). The trp operon is present within a histidine and aromatic amino acid supraoperon. In addition to the trp opero ...
... The B. subtilis trpEDCFBA operon contains six of the seven genes that are required for the biosynthesis of tryptophan from chorismic acid, the common aromatic amino acid precursor (Fig. 1). The trp operon is present within a histidine and aromatic amino acid supraoperon. In addition to the trp opero ...
P-Element Transformation with period Locus DNA Restores
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
Controlling morpholino experiments: don`t stop making antisense
... These problems in testing gene function delayed the field until early this century, when it was shown, with some fanfare [Editorial, Nature Genetics 26(2), 129-130], that MOs could be targeted to knockdown specific gene expression both in frog (Heasman et al., 2000) and in zebrafish (Ekker, 2000; Na ...
... These problems in testing gene function delayed the field until early this century, when it was shown, with some fanfare [Editorial, Nature Genetics 26(2), 129-130], that MOs could be targeted to knockdown specific gene expression both in frog (Heasman et al., 2000) and in zebrafish (Ekker, 2000; Na ...
Creation/Evolution - Geoscience Research Institute
... The net effect of wobble base pairing is to reduce the number of tRNAs that must be produced by a cell In reality cells do not make 61 different tRNAs, one for each codon Many tRNAs have anticodons that anneal to several different codons Codons are known for which there are more than one tRNA, altho ...
... The net effect of wobble base pairing is to reduce the number of tRNAs that must be produced by a cell In reality cells do not make 61 different tRNAs, one for each codon Many tRNAs have anticodons that anneal to several different codons Codons are known for which there are more than one tRNA, altho ...
DNA and RNA Extraction Controls Performance Summary
... of EDTA to the sample after extraction. The DEC not only serves as an indicator of the effectiveness of the extraction process, but can also be used to monitor co-purification of PCR inhibitors, as the DEC exhibits a similar profile of inhibition to the target gene www.bioline.com ...
... of EDTA to the sample after extraction. The DEC not only serves as an indicator of the effectiveness of the extraction process, but can also be used to monitor co-purification of PCR inhibitors, as the DEC exhibits a similar profile of inhibition to the target gene www.bioline.com ...
Rabbit Reticulocyte Lysate Technical Manual
... Rabbit Reticulocyte Lysate may contain a variety of post-translational processing activities, including acetylation, isoprenylation and some phosphorylation activity that will vary from lot to lot (2). Processing events such as signal peptide cleavage and core glycosylation can be examined by adding ...
... Rabbit Reticulocyte Lysate may contain a variety of post-translational processing activities, including acetylation, isoprenylation and some phosphorylation activity that will vary from lot to lot (2). Processing events such as signal peptide cleavage and core glycosylation can be examined by adding ...
Conceptual Questions C1. Answer: The start codon begins at the
... C11. Answer: An anticodon that was 3′–UUG–5′ would recognize the two codons. To recognize 5′–AAA–3′, it would have to be modified to 3′–UUI–5′. C12. Answer: All tRNA molecules have some basic features in common. They all have a cloverleaf structure with three stem-loop structures. The second stem-l ...
... C11. Answer: An anticodon that was 3′–UUG–5′ would recognize the two codons. To recognize 5′–AAA–3′, it would have to be modified to 3′–UUI–5′. C12. Answer: All tRNA molecules have some basic features in common. They all have a cloverleaf structure with three stem-loop structures. The second stem-l ...
Transcriptional Attenuation
... bacterial gene regulation, however, it has acquired a more restricted definition and is used to describe a mechanism in which the level of transcriptional termination at a single, specific site within an operon, called an attenuator, is regulated in response to a physiologically relevant signal (Bau ...
... bacterial gene regulation, however, it has acquired a more restricted definition and is used to describe a mechanism in which the level of transcriptional termination at a single, specific site within an operon, called an attenuator, is regulated in response to a physiologically relevant signal (Bau ...
Modeling tRNA*s Translator Function
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
Evolutionary Patterns in the Sequence and Structure of
... the evolution of closely related phytopathogenic fungi [17] or ...
... the evolution of closely related phytopathogenic fungi [17] or ...
Identification of functional domains in Arabidopsis thaliana mRNA
... has been proposed that the availability of the 7-methyl guanosine cap in mRNA is a dominant feature in competition for limited translation initiation factors, primarily eIF4E (1). Cap-independent translation mechanisms such as re-initiation and internal initiation generally lack the efficiency associa ...
... has been proposed that the availability of the 7-methyl guanosine cap in mRNA is a dominant feature in competition for limited translation initiation factors, primarily eIF4E (1). Cap-independent translation mechanisms such as re-initiation and internal initiation generally lack the efficiency associa ...
PDF File
... coworkers discovered the self-splicing activity of the group I intron from Tetrahymena thermophila (Kruger et al., 1982; Cech, 1992). The ability of RNA to serve as an information carrier is obvious, as it has the same code as DNA and is even used as such in viruses; RNA’s ability to serve a role an ...
... coworkers discovered the self-splicing activity of the group I intron from Tetrahymena thermophila (Kruger et al., 1982; Cech, 1992). The ability of RNA to serve as an information carrier is obvious, as it has the same code as DNA and is even used as such in viruses; RNA’s ability to serve a role an ...
Molecular Characterization of NADH-Dependent
... We therefore screened a second cDNA library by plaque hybridization using the 1.7-kb insert. A 765-bp BamHl fragment from the 5’end of the longest resulting cDNA (2.7 kb) was then used as a hybridization probe to screen a third cDNA library that had been prepared using a reverse transcriptase lackin ...
... We therefore screened a second cDNA library by plaque hybridization using the 1.7-kb insert. A 765-bp BamHl fragment from the 5’end of the longest resulting cDNA (2.7 kb) was then used as a hybridization probe to screen a third cDNA library that had been prepared using a reverse transcriptase lackin ...
Review over DNA, RNA, proteins, viruses, bacteria, DNA technology
... Essential knowledge 3.A.1: DNA, and in some cases RNA, is the primary source of heritable information. b. DNA and RNA molecules have structural similarities and differences that define function. [See also 4.A.1] Evidence of student learning is a demonstrated understanding of each of the following: 1 ...
... Essential knowledge 3.A.1: DNA, and in some cases RNA, is the primary source of heritable information. b. DNA and RNA molecules have structural similarities and differences that define function. [See also 4.A.1] Evidence of student learning is a demonstrated understanding of each of the following: 1 ...
Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
translation and protein structure
... Amino acids with polar side chains have a permanent charge separation, in which one end of the side chain is slightly more negatively charged than the other. As we saw in Chapter 2, polar molecules are hydrophilic, and they tend to form hydrogen bonds with each other or with water molecules. The sid ...
... Amino acids with polar side chains have a permanent charge separation, in which one end of the side chain is slightly more negatively charged than the other. As we saw in Chapter 2, polar molecules are hydrophilic, and they tend to form hydrogen bonds with each other or with water molecules. The sid ...
Strong association between mRNA folding strength and protein
... sequence biases, (B) the protocol has a very small bias towards particular regions along the transcript; (C) the protocol captures RNA fragments in proportion to their abundance in the initial pool; (D) the signals generated by RNase V1 (that measures basepair conformation) are highly distinct from ...
... sequence biases, (B) the protocol has a very small bias towards particular regions along the transcript; (C) the protocol captures RNA fragments in proportion to their abundance in the initial pool; (D) the signals generated by RNase V1 (that measures basepair conformation) are highly distinct from ...
Lehninger Principles of Biochemistry
... thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broader range of functions, and several classes are found in cells. Ribosomal RNAs (rRNAs) are components of ribosom ...
... thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broader range of functions, and several classes are found in cells. Ribosomal RNAs (rRNAs) are components of ribosom ...
Sense and Nonsense in the Genetic Code
... The recent elucidation of the genetic code, shown in Table 1 marks a notable milestone in biology (1). This code designates the relations between the 64 possible codons (2) present in messenger RNA and the 20 amino acids present in proteins. The RNA codons are derived by transcription of com?lementa ...
... The recent elucidation of the genetic code, shown in Table 1 marks a notable milestone in biology (1). This code designates the relations between the 64 possible codons (2) present in messenger RNA and the 20 amino acids present in proteins. The RNA codons are derived by transcription of com?lementa ...
Single Processing Center Models for Human Dicer and Bacterial
... function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated ...
... function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated ...
RNA
Ribonucleic acid (RNA) is a polymeric molecule implicated in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the three major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, U, A, and C to denote the nitrogenous bases guanine, uracil, adenine, and cytosine) that directs synthesis of specific proteins. Many viruses encode their genetic information using an RNA genome.Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals. One of these active processes is protein synthesis, a universal function whereby mRNA molecules direct the assembly of proteins on ribosomes. This process uses transfer RNA (tRNA) molecules to deliver amino acids to the ribosome, where ribosomal RNA (rRNA) links amino acids together to form proteins.