c-Myc co-ordinates mRNA cap methylation and ribosomal RNA
... Figure 1. Expression of the mRNA cap methyltransferase complex, RNMT–RAM, is required for 45S rRNA production. HeLa cells were transfected into two independent RAM siRNAs, an RNMT siRNA and a non-targeting control siRNA, for 48 h. (A) Expression of RNMT, RAM and GAPDH was analysed by western blot. ( ...
... Figure 1. Expression of the mRNA cap methyltransferase complex, RNMT–RAM, is required for 45S rRNA production. HeLa cells were transfected into two independent RAM siRNAs, an RNMT siRNA and a non-targeting control siRNA, for 48 h. (A) Expression of RNMT, RAM and GAPDH was analysed by western blot. ( ...
Testing the ABC floral-organ identity model: expression of
... Clone and sequence the ABC genes. Look for similarities with sequenced genes already in the database. ...
... Clone and sequence the ABC genes. Look for similarities with sequenced genes already in the database. ...
Unsaturated Fatty Acids, Desaturases, and Human Health
... bond from the carboxylic end in a fatty acid chain. Delta-6deasturase, a rate-limiting desaturase for the production of PUFA, is known to serve multiple functions, including delta-6 desaturation activities on LA and ALA (Fig. 1B).2,10 In addition to the delta-6 desaturase activity, the enzyme exhibi ...
... bond from the carboxylic end in a fatty acid chain. Delta-6deasturase, a rate-limiting desaturase for the production of PUFA, is known to serve multiple functions, including delta-6 desaturation activities on LA and ALA (Fig. 1B).2,10 In addition to the delta-6 desaturase activity, the enzyme exhibi ...
Monoallelic Expression and Dominance
... posures of RNA gel blots and quantitative reverse transcriptase-PCR, respectively. In contrast, SCRb transcripts were reduced by only approximately 10% in heterozygotes relative to Sb homozygotes. Importantly, comparison of eight SaSa and eight SaSb plants generated by forced selfing of an SaSb plan ...
... posures of RNA gel blots and quantitative reverse transcriptase-PCR, respectively. In contrast, SCRb transcripts were reduced by only approximately 10% in heterozygotes relative to Sb homozygotes. Importantly, comparison of eight SaSa and eight SaSb plants generated by forced selfing of an SaSb plan ...
Genome Evolution and Developmental Constraint in Caenorhabditis
... It has been hypothesized that evolutionary changes will be more frequent in later ontogeny than early ontogeny because of developmental constraint. To test this hypothesis, a genomewide examination of molecular evolution through ontogeny was carried out using comparative genomic data in Caenorhabdit ...
... It has been hypothesized that evolutionary changes will be more frequent in later ontogeny than early ontogeny because of developmental constraint. To test this hypothesis, a genomewide examination of molecular evolution through ontogeny was carried out using comparative genomic data in Caenorhabdit ...
- eScholarship@UMMS - University of Massachusetts
... fibroblast cell lines were used in this study: an HGPS patient fibroblast (HGPS), a normal cell line from the father of the HGPS patient (Father), and an age-matched normal fibroblast line (Age Control). Two biological replicates were performed at different passages, since some changes in HGPS may p ...
... fibroblast cell lines were used in this study: an HGPS patient fibroblast (HGPS), a normal cell line from the father of the HGPS patient (Father), and an age-matched normal fibroblast line (Age Control). Two biological replicates were performed at different passages, since some changes in HGPS may p ...
1 Oviduct-embryo interactions in cattle
... evidence for a positive influence of the oviduct on the quality of the early embryo. For example, short term culture of in vitro produced bovine zygotes in the oviducts of cattle [9, 10], sheep [11, 12] or even mice [13] has been shown to improve embryo quality measured in terms of morphology, gene ...
... evidence for a positive influence of the oviduct on the quality of the early embryo. For example, short term culture of in vitro produced bovine zygotes in the oviducts of cattle [9, 10], sheep [11, 12] or even mice [13] has been shown to improve embryo quality measured in terms of morphology, gene ...
SpliceCenter_DataBuild
... are accepted if they contain a CDS section that identifies a coding start/stop position. In this case, the sequence must also contain canonical start/stop codons at the indicated positions. Accepted sequences are written to the /build//trans director as individual FASTA files. In
addition ...
... are accepted if they contain a CDS section that identifies a coding start/stop position. In this case, the sequence must also contain canonical start/stop codons at the indicated positions. Accepted sequences are written to the /build/
Coexpression of Linked Genes in Mammalian
... Here, we propose that coexpression of linked genes is a form of transcriptional interference that is disadvantageous to the organism. We show that even distantly linked genes that are tens of megabases away exhibit significant coexpression in the human genome. However, the linkage is more likely to ...
... Here, we propose that coexpression of linked genes is a form of transcriptional interference that is disadvantageous to the organism. We show that even distantly linked genes that are tens of megabases away exhibit significant coexpression in the human genome. However, the linkage is more likely to ...
A Bayesian Network Classification Methodology for Gene
... is labeled—associating with each training case the class to which it belongs—supports statistical methods for constructing a classifier. After training on a collection of labeled data, a classifier is constructed which, when presented with new query cases, predicts a class label from gene expression ...
... is labeled—associating with each training case the class to which it belongs—supports statistical methods for constructing a classifier. After training on a collection of labeled data, a classifier is constructed which, when presented with new query cases, predicts a class label from gene expression ...
A Case Study of Leopard Appaloosa Alpacas in one
... lighter colour infused by spots of the same colour as the rest of the coat, the term ‘Leopard’ is used to describe coloured spots evenly distributed over the entire body (Wilpedia, 2009). This pattern of colour occurs in a wide range of species, including the horse, dogs (ie dalmations), moths and f ...
... lighter colour infused by spots of the same colour as the rest of the coat, the term ‘Leopard’ is used to describe coloured spots evenly distributed over the entire body (Wilpedia, 2009). This pattern of colour occurs in a wide range of species, including the horse, dogs (ie dalmations), moths and f ...
Gene methylation in gastric cancer
... Age is an important risk factor in the development of gastric cancer. Tumor-related genes are rarely methylated in nonneoplastic gastric epithelia from young people, which, in contrast, are frequently found in older people. The association between decreasing global DNA methylation and aging has been ...
... Age is an important risk factor in the development of gastric cancer. Tumor-related genes are rarely methylated in nonneoplastic gastric epithelia from young people, which, in contrast, are frequently found in older people. The association between decreasing global DNA methylation and aging has been ...
Genome-wide association studies for complex traits: consensus
... which the effect of misclassification on power is greatest1, one remedy involves adopting a more stringent case definition, for example, based on early age of onset or ascertainment of a more extreme phenotype, while still excluding monogenic cases. Although the most powerful strategy for a given fi ...
... which the effect of misclassification on power is greatest1, one remedy involves adopting a more stringent case definition, for example, based on early age of onset or ascertainment of a more extreme phenotype, while still excluding monogenic cases. Although the most powerful strategy for a given fi ...
outline4003
... Vision more severely affected than in other stromal dystrophies Characterized by stromal haze, and milky white opacities (glucosamineglycans) Progresses to corneal periphery by ages 20-30 By age 40, PK may be required Mutation localized to the 123 gene on chromosome 16 (16q22) Central Crystalline Dy ...
... Vision more severely affected than in other stromal dystrophies Characterized by stromal haze, and milky white opacities (glucosamineglycans) Progresses to corneal periphery by ages 20-30 By age 40, PK may be required Mutation localized to the 123 gene on chromosome 16 (16q22) Central Crystalline Dy ...
Annotation Instruction Sheet A. Information for Beginning Annotators
... intron/exon boundaries. However there are important things to consider when evaluating expression data. It is important to remember that some genes are expressed at very low levels or only for very short periods of time. As such the lack of expression data for any gene should not be considered stron ...
... intron/exon boundaries. However there are important things to consider when evaluating expression data. It is important to remember that some genes are expressed at very low levels or only for very short periods of time. As such the lack of expression data for any gene should not be considered stron ...
Supplementary Discussion References
... transcription which made up to 50 µg of cRNA. The unfragmented cRNA was tested by running on an Agilent electropherogram to measure the size distribution. The median size (nucleotide length) of the cRNA is reported in Supplementary Table 1. If a sample gave low amounts of cRNA by spectrophotometer o ...
... transcription which made up to 50 µg of cRNA. The unfragmented cRNA was tested by running on an Agilent electropherogram to measure the size distribution. The median size (nucleotide length) of the cRNA is reported in Supplementary Table 1. If a sample gave low amounts of cRNA by spectrophotometer o ...
Genetics of fibrosing lung diseases REVIEW
... studies is the need for substantial sample sizes. The second strategy is based on case–control association studies, which seek evidence for a significant association between an allele of a candidate gene or gene haplotype (series of polymorphisms in the same gene that cosegregate) and a disease/phen ...
... studies is the need for substantial sample sizes. The second strategy is based on case–control association studies, which seek evidence for a significant association between an allele of a candidate gene or gene haplotype (series of polymorphisms in the same gene that cosegregate) and a disease/phen ...
Temperature-dependent expression of virulence genes in fish
... 2010; Steinmann and Dersch, 2013). In addition, bacteria also have response mechanisms to drastic changes in temperature. The paradigms of this adaptation are the cold and heat shock response systems under which the bacteria induce a fast response to sudden and extreme temperature shift (Guisbert et ...
... 2010; Steinmann and Dersch, 2013). In addition, bacteria also have response mechanisms to drastic changes in temperature. The paradigms of this adaptation are the cold and heat shock response systems under which the bacteria induce a fast response to sudden and extreme temperature shift (Guisbert et ...
Mendel`s Genes: Toward a Full Molecular Characterization
... are amenable to candidate gene approaches on the basis of their function, linkage relationships, and synteny between the pea and Medicago genomes. However, even the gene (locus) identity is not known for certain for the seventh character, the pod form, although it is probably V. While the nature of ...
... are amenable to candidate gene approaches on the basis of their function, linkage relationships, and synteny between the pea and Medicago genomes. However, even the gene (locus) identity is not known for certain for the seventh character, the pod form, although it is probably V. While the nature of ...
the full article - Medical Writing
... overweight and obese, whereas some of the overweight can’t manage to lose weight. This suggests that in a population exposed to the same environment, there is inter-individual variation in obesity susceptibility, suggesting that biological mechanisms or genes influence obesity. Obesity is known to r ...
... overweight and obese, whereas some of the overweight can’t manage to lose weight. This suggests that in a population exposed to the same environment, there is inter-individual variation in obesity susceptibility, suggesting that biological mechanisms or genes influence obesity. Obesity is known to r ...
The Value of MLPA in Waardenburg Syndrome - MRC
... Since PAX3 was cloned (Baldwin et al., 1992), no other gene has been discovered as an etiology for WS 1 or 3. Point mutations in PAX3 have been identified in more than 90% of affected individuals with WS 1 or 3. In contrast, WS2 is genetically heterogeneous, with only 10–15% of affected individuals ...
... Since PAX3 was cloned (Baldwin et al., 1992), no other gene has been discovered as an etiology for WS 1 or 3. Point mutations in PAX3 have been identified in more than 90% of affected individuals with WS 1 or 3. In contrast, WS2 is genetically heterogeneous, with only 10–15% of affected individuals ...
REVIEW ARTICLE Gene cassettes
... integron, and thus form a family of mobile elements that are more akin to integrating phage and plasmids than to transposons. Cassettes do not, in general, include a promoter and are transcribed from a promoter in the integron. The multi-component cassette-integron system is thus a natural cloning a ...
... integron, and thus form a family of mobile elements that are more akin to integrating phage and plasmids than to transposons. Cassettes do not, in general, include a promoter and are transcribed from a promoter in the integron. The multi-component cassette-integron system is thus a natural cloning a ...
Gene Duplication, Gene Conversion and the Evolution of
... coupled with gene conversion between duplicate pairs, can potentially counteract forces of evolutionary decay that accompany asexual reproduction. Using a combination of analytical and computer simulation methods, we explicitly show that, although gene conversion has little impact on the probability ...
... coupled with gene conversion between duplicate pairs, can potentially counteract forces of evolutionary decay that accompany asexual reproduction. Using a combination of analytical and computer simulation methods, we explicitly show that, although gene conversion has little impact on the probability ...
mutation and Y486D mutation in exon 5 of the UGT1A1 - Funpec-RP
... causes of Crigler-Najjar (CN) syndrome and Gilbert’s syndrome (Canu et al., 2013). The TATA promoter plays a critical role in controlling the expression of the UGT1A1 gene. An insertion mutation with the nucleotide base “A” in the TATA promoter region leads to common mild hyperbilirubinemia, also kn ...
... causes of Crigler-Najjar (CN) syndrome and Gilbert’s syndrome (Canu et al., 2013). The TATA promoter plays a critical role in controlling the expression of the UGT1A1 gene. An insertion mutation with the nucleotide base “A” in the TATA promoter region leads to common mild hyperbilirubinemia, also kn ...