Involvement of Pax6 and Otx2 in the forebrain
... species, this element is also active in the corresponding expression domain of the zebrafish orthologue. In vivo analysis of two in vitroidentified Otx2 binding sites in this conserved sequence revealed their necessity for activation of the chicken ANF promoter. In addition, we identified a Pax6-bin ...
... species, this element is also active in the corresponding expression domain of the zebrafish orthologue. In vivo analysis of two in vitroidentified Otx2 binding sites in this conserved sequence revealed their necessity for activation of the chicken ANF promoter. In addition, we identified a Pax6-bin ...
Adiponectin mRNA Expression in the Cat (Felis domesticus)
... humans and maybe subcutaneous adipose depots may contribute more to the development of insulin resistance and diabetes in cats. The results of this study are intriguing and provide the opportunity for many additional projects. It would be interesting to compare adiponectin expression patterns in adi ...
... humans and maybe subcutaneous adipose depots may contribute more to the development of insulin resistance and diabetes in cats. The results of this study are intriguing and provide the opportunity for many additional projects. It would be interesting to compare adiponectin expression patterns in adi ...
FEBS Letters
... (coding for the larger subunit of the diaphorase part) could not be detected either as a direct sequence on the 5 kb segment or by heterologous probing with the A. variabilis gene on a 13 kb )~-clone containing the 5 kb segment. The hoxF gene could, however, be identified on genomic D N A cleaved wi ...
... (coding for the larger subunit of the diaphorase part) could not be detected either as a direct sequence on the 5 kb segment or by heterologous probing with the A. variabilis gene on a 13 kb )~-clone containing the 5 kb segment. The hoxF gene could, however, be identified on genomic D N A cleaved wi ...
Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in
... efficient expression and subcellular localisation, we here engineer the baker’s yeast Saccharomyces cerevisiae to produce and secrete the antibiotic penicillin, a betalactam nonribosomal peptide. Using synthetic biology tools combined with long-read DNA sequencing, we optimise productivity by 50-fol ...
... efficient expression and subcellular localisation, we here engineer the baker’s yeast Saccharomyces cerevisiae to produce and secrete the antibiotic penicillin, a betalactam nonribosomal peptide. Using synthetic biology tools combined with long-read DNA sequencing, we optimise productivity by 50-fol ...
University of Groningen Methionine
... The importance of methionine acquisition and synthesis for S. pneumoniae growth and virulence has been reported before (Basavanna et al., 2013). In this study, we explored the impact of methionine on the transcriptome of S. pneumoniae D39. To do so, we performed transcriptome comparison of S. pneumo ...
... The importance of methionine acquisition and synthesis for S. pneumoniae growth and virulence has been reported before (Basavanna et al., 2013). In this study, we explored the impact of methionine on the transcriptome of S. pneumoniae D39. To do so, we performed transcriptome comparison of S. pneumo ...
ABO Blood Group System
... ABO compatibility between donor cell and patient serum is the essential foundation of pre-transfusion testing It is the only system with expected antibodies Whether they are IgG or IgM, ABO antibodies can activate complement readily ...
... ABO compatibility between donor cell and patient serum is the essential foundation of pre-transfusion testing It is the only system with expected antibodies Whether they are IgG or IgM, ABO antibodies can activate complement readily ...
Comprehensive and Rapid Genotyping of Mutations - HAL
... developing two methods: i) a rapid and robust direct sequence analysis of all exons/flanking introns of the CFTR gene based on single condition touchdown amplification/sequencing in 96-well plates, ii) a fluorescent assay which allows haplotyping of IVS8-(TG)mTn even without family linkage study. Co ...
... developing two methods: i) a rapid and robust direct sequence analysis of all exons/flanking introns of the CFTR gene based on single condition touchdown amplification/sequencing in 96-well plates, ii) a fluorescent assay which allows haplotyping of IVS8-(TG)mTn even without family linkage study. Co ...
An assessment of the risks associated with the
... ation, since it is generally accepted that all DNA sequences behave chemically in the same way, in that there is no history of any piece of DNA being toxic to humans or any other animal and that large quantities of DNA are eaten by everyone on the planet on a daily basis as an integral component of ...
... ation, since it is generally accepted that all DNA sequences behave chemically in the same way, in that there is no history of any piece of DNA being toxic to humans or any other animal and that large quantities of DNA are eaten by everyone on the planet on a daily basis as an integral component of ...
CSCE590/822 Data Mining Principles and Applications
... Cluster may represent a group of functionally related genes (Biological Module). Possible Drawbacks with Clustering: ...
... Cluster may represent a group of functionally related genes (Biological Module). Possible Drawbacks with Clustering: ...
Prof. Kamakaka`s Lecture 16 Notes
... In addition to negative regulation by the lac repressor, the lac operon also required positive regulation. That is, a factor was needed for specific activation of the operon. The specific activator was called CAP ( Catabolic activator protein) produced by the gene crp (not part of the lac operon). C ...
... In addition to negative regulation by the lac repressor, the lac operon also required positive regulation. That is, a factor was needed for specific activation of the operon. The specific activator was called CAP ( Catabolic activator protein) produced by the gene crp (not part of the lac operon). C ...
Cancer Prone Disease Section Multiple osteochondromas (MO) Atlas of Genetics and Cytogenetics
... Genes involved and proteins Note MO is a genetically heterogeneous disorder for which at present, two genes, 1 and 2 located respectively on 8q24 and 11p11-p12, have been isolated. The EXT1 gene was reported to show linkage in 44%-66% of the MO families, whereas EXT2 would be involved in ...
... Genes involved and proteins Note MO is a genetically heterogeneous disorder for which at present, two genes, 1 and 2 located respectively on 8q24 and 11p11-p12, have been isolated. The EXT1 gene was reported to show linkage in 44%-66% of the MO families, whereas EXT2 would be involved in ...
Recurrent patterns of DNA copy number alterations in tumors reflect
... of clusters, (i) so more information on how this methodology applies here is warranted. (ii) Which CNAs/amplicons do PC 1, 2, 3, and 4 actually separate, or do they merely separate copy-number stable from more instable (p53 mutated) tumors? (iii) Currently, the authors discuss PC1 (principal compone ...
... of clusters, (i) so more information on how this methodology applies here is warranted. (ii) Which CNAs/amplicons do PC 1, 2, 3, and 4 actually separate, or do they merely separate copy-number stable from more instable (p53 mutated) tumors? (iii) Currently, the authors discuss PC1 (principal compone ...
Growth medium composition-determined regulatory mechanisms
... Expression of the phenol degradation pathway in Pseudomonas putida strain PaW85 requires coordinated transcription of the plasmid-borne pheBA operon encoding catechol 1,2-dioxygenase and phenol monooxygenase, respectively, and the chromosomally encoded catechol degradation catBCA operon. Transcripti ...
... Expression of the phenol degradation pathway in Pseudomonas putida strain PaW85 requires coordinated transcription of the plasmid-borne pheBA operon encoding catechol 1,2-dioxygenase and phenol monooxygenase, respectively, and the chromosomally encoded catechol degradation catBCA operon. Transcripti ...
Genes for Cognitive Function: Developments on the X
... established, and additions remain ongoing. In the recent past this was the point when the family study was abandoned. The gene localization determined by linkage in single families was too broad for positional cloning; very few of the potential candidate genes had been discovered, and there were no ...
... established, and additions remain ongoing. In the recent past this was the point when the family study was abandoned. The gene localization determined by linkage in single families was too broad for positional cloning; very few of the potential candidate genes had been discovered, and there were no ...
Axial homeosis and appendicular skeleton defects in mice with a
... outgrowth of the limb bud is controlled by the apical ectodermal ridge (AER), which maintains the underlying mesoderm (the progress zone) in a highly proliferative state. As the limb grows, cells leave the progress zone and participate in forming the prechondrogenic condensations that generate the l ...
... outgrowth of the limb bud is controlled by the apical ectodermal ridge (AER), which maintains the underlying mesoderm (the progress zone) in a highly proliferative state. As the limb grows, cells leave the progress zone and participate in forming the prechondrogenic condensations that generate the l ...
Applicability of Yeast Genetics to Neurologic Disease
... including p53. Chk2 then phosphorylates Cdc25C, blocking its key role in initiating mitosis by dephosphorylation of Cdc2. This signaling pathway is quite similar in yeast, where DNA damage causes the Atm homolog, Mec1, to phosphorylate Rad53/Spk1. In fact, human Chk2 was found because the yeast syst ...
... including p53. Chk2 then phosphorylates Cdc25C, blocking its key role in initiating mitosis by dephosphorylation of Cdc2. This signaling pathway is quite similar in yeast, where DNA damage causes the Atm homolog, Mec1, to phosphorylate Rad53/Spk1. In fact, human Chk2 was found because the yeast syst ...
Floral Symmetry - Coen Lab
... structure of promoter or regulatory regions. The question then became what factors determined the prepattern. A candidate prepattern gene for flower colour was delila (del). The five petals of an Antirrhinum flower are united for part of their length to form a tube, as distinct from the petal lobes ...
... structure of promoter or regulatory regions. The question then became what factors determined the prepattern. A candidate prepattern gene for flower colour was delila (del). The five petals of an Antirrhinum flower are united for part of their length to form a tube, as distinct from the petal lobes ...
abstracts
... Our HortResearch fruit EST database contains over 160,000 apple sequences from 62 cDNA libraries representing several cultivars and a range of tissues. Exploitation of this resource is supported by a range of enabling technologies, including bioinformatics, biochemistry, physiology, in vivo function ...
... Our HortResearch fruit EST database contains over 160,000 apple sequences from 62 cDNA libraries representing several cultivars and a range of tissues. Exploitation of this resource is supported by a range of enabling technologies, including bioinformatics, biochemistry, physiology, in vivo function ...
Profiling genes expressed in human fetal cartilage using 13,155
... with IGF-II, its receptor (four copies) and four different binding proteins (IGFBP) were identified, including IGFBP2 (one copy), IGFBP3 (two copies), IGFBP4 (one copy) and IGFBP5 (six copies). The second most abundant growth factor was connective growth factor (CTGF) with six EST copies. Two transf ...
... with IGF-II, its receptor (four copies) and four different binding proteins (IGFBP) were identified, including IGFBP2 (one copy), IGFBP3 (two copies), IGFBP4 (one copy) and IGFBP5 (six copies). The second most abundant growth factor was connective growth factor (CTGF) with six EST copies. Two transf ...
Tracking the evolution of 3D gene organization demonstrates its
... regulation (4,9), thus divergence in gene expression is expected to be reflected in 3D gene organization (21). Recently, tools for studying differential Hi-C contacts have recently been proposed, and applied to the study of genomic organization in a cancer cell line (22,23), but to the best of our k ...
... regulation (4,9), thus divergence in gene expression is expected to be reflected in 3D gene organization (21). Recently, tools for studying differential Hi-C contacts have recently been proposed, and applied to the study of genomic organization in a cancer cell line (22,23), but to the best of our k ...
HMG 9_9.book(ddd146.fm)
... MECP2 gene in 46 typical RTT patients and screened by DGGE (n = 38) and by direct sequencing (n = 8) the whole coding sequence of this gene. Upon analysis by DGGE of exon 1 to exon 3, and sequencing of PCR fragments exhibiting abnormal DGGE migration profiles, we have identified 25/38 (66%). Direct ...
... MECP2 gene in 46 typical RTT patients and screened by DGGE (n = 38) and by direct sequencing (n = 8) the whole coding sequence of this gene. Upon analysis by DGGE of exon 1 to exon 3, and sequencing of PCR fragments exhibiting abnormal DGGE migration profiles, we have identified 25/38 (66%). Direct ...
RNA-Seq analysis
... RNA-Seq technology -Aims: Catalogue all species of transcript including: mRNAs, non-coding RNAs and small RNAs Determine the transcriptional structure of genes in terms of: Start sites 5′ and 3′ ends Splicing patterns Other post-transcriptional modifications Quantification of expressi ...
... RNA-Seq technology -Aims: Catalogue all species of transcript including: mRNAs, non-coding RNAs and small RNAs Determine the transcriptional structure of genes in terms of: Start sites 5′ and 3′ ends Splicing patterns Other post-transcriptional modifications Quantification of expressi ...
CRISPR-Cas9 Mouse Toolbox
... an sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence (20bp). The cloning protocol can be found below. ...
... an sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence (20bp). The cloning protocol can be found below. ...