Narcissus pseudonarcissus L. `Dutch Master`
... tepals of D1 and D4 flowers confirmed that many of the sequences (or closely related homologs) were more highly expressed in D4 flowers (data not shown). These sequences are indicated in Table 1 by an asterisk. Conventional RNA gel blot analysis was used to examine the time course of upregulation of ...
... tepals of D1 and D4 flowers confirmed that many of the sequences (or closely related homologs) were more highly expressed in D4 flowers (data not shown). These sequences are indicated in Table 1 by an asterisk. Conventional RNA gel blot analysis was used to examine the time course of upregulation of ...
Genome-Wide RNAi Screen for Host Factors Required for
... infection by DH-L1137 (fig. S1A) and is consistent with a defect in vacuole escape. However, bacteria in dsRNA treated wells appeared to be capable of increased replication within the localized clusters (Fig. 1E). (iii) Increased GFP fluorescence (Up phenotype). The intensity of GFP fluorescence per ...
... infection by DH-L1137 (fig. S1A) and is consistent with a defect in vacuole escape. However, bacteria in dsRNA treated wells appeared to be capable of increased replication within the localized clusters (Fig. 1E). (iii) Increased GFP fluorescence (Up phenotype). The intensity of GFP fluorescence per ...
RNA Express Workflow - support.illumina.com
... expression, fold change, standard error, p-value, etc.) is reported for each gene. The script writes a table of raw counts across all replicates and plots a gene-level heat map sorted by hierarchical clustering. This heat map contains up to 5000 significantly differentially expressed genes, q < 0.05 ...
... expression, fold change, standard error, p-value, etc.) is reported for each gene. The script writes a table of raw counts across all replicates and plots a gene-level heat map sorted by hierarchical clustering. This heat map contains up to 5000 significantly differentially expressed genes, q < 0.05 ...
Emerging Roles for Non-Coding RNAs in Male Reproductive
... A. thaliana and O. sativa pollen for a number of known and new microRNAs [15,21,24,25]. Intriguingly, the microRNA-triggered phased siRNAs reported from rice inflorescence and anther development do not appear to act on their targets through a mechanism of cleavage, and instead may downregulate targe ...
... A. thaliana and O. sativa pollen for a number of known and new microRNAs [15,21,24,25]. Intriguingly, the microRNA-triggered phased siRNAs reported from rice inflorescence and anther development do not appear to act on their targets through a mechanism of cleavage, and instead may downregulate targe ...
Document
... • These noncoding regions are called intervening sequences, or ____________ • The other regions are called ____________ because they are eventually expressed, usually translated into amino acid sequences • _____________________removes introns and joins exons, creating an mRNA molecule with a ...
... • These noncoding regions are called intervening sequences, or ____________ • The other regions are called ____________ because they are eventually expressed, usually translated into amino acid sequences • _____________________removes introns and joins exons, creating an mRNA molecule with a ...
Supplementary Material Legends
... Table S1. T-DNA insertion sites of target transgenes are defined by NCBI accession (acc.) numbers for left border or right border T-DNA - A. thaliana chromosomal DNA fusion sequences. Positions of nucleotides of A. thaliana genomic BAC sequences adjacent to left border and right border T-DNA sequenc ...
... Table S1. T-DNA insertion sites of target transgenes are defined by NCBI accession (acc.) numbers for left border or right border T-DNA - A. thaliana chromosomal DNA fusion sequences. Positions of nucleotides of A. thaliana genomic BAC sequences adjacent to left border and right border T-DNA sequenc ...
Pair-rule genes
... Activator domains may interact with coactivators that in turn bind to components of the basal apparatus. Repressor domains also interact with the transcription apparatus. The repressor Eve, for example, interacts directly with TFIID. ...
... Activator domains may interact with coactivators that in turn bind to components of the basal apparatus. Repressor domains also interact with the transcription apparatus. The repressor Eve, for example, interacts directly with TFIID. ...
HA Tagged Yeast Clones and Collection - Technical
... through triple hemagglutinin epitope tag (3xHA tag), remains. The mTn technology has proven useful for large-scale functional analysis of the yeast genome. The HA tags permit a variety of functional studies including immunolocalization, immunoprecipitation and analysis of binding sites using immuno ...
... through triple hemagglutinin epitope tag (3xHA tag), remains. The mTn technology has proven useful for large-scale functional analysis of the yeast genome. The HA tags permit a variety of functional studies including immunolocalization, immunoprecipitation and analysis of binding sites using immuno ...
VeriScript™ Reverse Transcriptase
... HeLa total RNA (1 µg) was used in a 20 µl first-strand cDNA synthesis with 200 units of VeriScript Reverse Transcriptase. Reactions were incubated at the temperatures indicated on the figure for 30 minutes, followed by heat inactivation for 15 minutes at 70°C. RNA was removed by adding 1 µl (5 units ...
... HeLa total RNA (1 µg) was used in a 20 µl first-strand cDNA synthesis with 200 units of VeriScript Reverse Transcriptase. Reactions were incubated at the temperatures indicated on the figure for 30 minutes, followed by heat inactivation for 15 minutes at 70°C. RNA was removed by adding 1 µl (5 units ...
DNA-free CRISPR-Cas9 genome engineering in
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
study guide for Vlad
... an experimental technique used to identify and select for individuals who possess a phenotype of interest (Wikipedia) A 180 base pair sequence in DNA that codes for a binding domain. Is important in controlling morphological patterns in animal, fungal, and plant development. (Wikipedia/Freeman text) ...
... an experimental technique used to identify and select for individuals who possess a phenotype of interest (Wikipedia) A 180 base pair sequence in DNA that codes for a binding domain. Is important in controlling morphological patterns in animal, fungal, and plant development. (Wikipedia/Freeman text) ...
DNA transcription 3.lecture ENG OK
... The TATA box , often TATAAA and usually found 25-30 base pairs upstream (-25) from the start site of transcription. Only about 10-15% of mammalian genes contain TATA boxes, while the rest contain other core promoter elements. The GC box , often GGGCGG, found in a variety of genes, many lacking a ...
... The TATA box , often TATAAA and usually found 25-30 base pairs upstream (-25) from the start site of transcription. Only about 10-15% of mammalian genes contain TATA boxes, while the rest contain other core promoter elements. The GC box , often GGGCGG, found in a variety of genes, many lacking a ...
Supplemental Material 1 Simultaneous isolation of mRNA, miRNA
... expressed as [(All MREs in transcript X for all miRs predicted to target X’s 3’UTR) - (# of miRs predicted to target X’s 3’UTR + 1)] / (MREs in X’s 3’UTR for all considered miRs). This criteria rewards transcripts for having a higher number of predicted MREs for each targeting miR. As the number of ...
... expressed as [(All MREs in transcript X for all miRs predicted to target X’s 3’UTR) - (# of miRs predicted to target X’s 3’UTR + 1)] / (MREs in X’s 3’UTR for all considered miRs). This criteria rewards transcripts for having a higher number of predicted MREs for each targeting miR. As the number of ...
Transcription
... Some gene products are needed by all cells all the time. These constitutive genes are expressed by all cells. Other genes are only needed by certain cells or at specific times, expression of these inducible genes is tightly controlled in most cells. For example, pancreatic b cells make insulin by ex ...
... Some gene products are needed by all cells all the time. These constitutive genes are expressed by all cells. Other genes are only needed by certain cells or at specific times, expression of these inducible genes is tightly controlled in most cells. For example, pancreatic b cells make insulin by ex ...
Klotho interferes with a novel FGF-signalling pathway and
... gene products (479 and 475 amino-acids, respectively) are 76% identical and share 32 to 34% amino acid identity with their mammalian counterparts. Interestingly, both C. elegans predicted gene products consist of a sole β-glucosidase-like domain that is homologous to the highly conserved KL1 domain ...
... gene products (479 and 475 amino-acids, respectively) are 76% identical and share 32 to 34% amino acid identity with their mammalian counterparts. Interestingly, both C. elegans predicted gene products consist of a sole β-glucosidase-like domain that is homologous to the highly conserved KL1 domain ...
Inquiry into Life Twelfth Edition
... or depress, respectively, transcription of associated genes • Are often tissue-specific in that they rely on tissue-specific DNA-binding proteins for their activities • Some DNA elements can act either as enhancer or silencer depending on what is bound to it ...
... or depress, respectively, transcription of associated genes • Are often tissue-specific in that they rely on tissue-specific DNA-binding proteins for their activities • Some DNA elements can act either as enhancer or silencer depending on what is bound to it ...
Handouts
... The Yoneda lemma allows the embedding of any category into a category of functors defined on that category. It suggests that instead of studying the (small) category C, one should study the category o ...
... The Yoneda lemma allows the embedding of any category into a category of functors defined on that category. It suggests that instead of studying the (small) category C, one should study the category o ...
RACC BIO transcription and translation
... • The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins and of RNA molecules involved in protein synthesis. • Gene expression is the process by which DNA directs the synthesis of proteins. • The process by which DNA directs protein synthesis, gene expressio ...
... • The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins and of RNA molecules involved in protein synthesis. • Gene expression is the process by which DNA directs the synthesis of proteins. • The process by which DNA directs protein synthesis, gene expressio ...
Full Text
... sequences that we call GtPax6A. Comparative sequence analysis confirms that both planarian genes are bona fide Pax6, conserving specific residues of Pax6 family in the paired and homedomain regions. Such duplication is not homologous to the Pax6 duplication observed in insects, and can be considered ...
... sequences that we call GtPax6A. Comparative sequence analysis confirms that both planarian genes are bona fide Pax6, conserving specific residues of Pax6 family in the paired and homedomain regions. Such duplication is not homologous to the Pax6 duplication observed in insects, and can be considered ...
PCR settings, pitfalls and artefacts
... but only a portion of the cDNA products is used as the template for PCR, which is performed in a separate tube. ...
... but only a portion of the cDNA products is used as the template for PCR, which is performed in a separate tube. ...
Protein synthesis File
... Transfer RNA (tRNA) transports amino acids to the ribosome for incorporation into protein. Each tRNA is a single polynucleotide that is folded into a clover-leaf shape. Hydrogen bonds between complementary bases stabilise the shape. tRNA has: an anticodon that is complementary to the codon ...
... Transfer RNA (tRNA) transports amino acids to the ribosome for incorporation into protein. Each tRNA is a single polynucleotide that is folded into a clover-leaf shape. Hydrogen bonds between complementary bases stabilise the shape. tRNA has: an anticodon that is complementary to the codon ...
File
... Small nuclear RNA, forms complexes with proteins used in eukaryotic RNA processing (e.g., exon splicing and intron removal). ...
... Small nuclear RNA, forms complexes with proteins used in eukaryotic RNA processing (e.g., exon splicing and intron removal). ...
Measurement of flowering time
... 2006). It remains to be shown whether any of the three tomato members of CO family group Ia plays a role in tomato flowering, since the results reported so far do not exclude this possibility (Ben-Naim et al., 2006). Whether other CO family genes from potato have a stronger effect on flowering is st ...
... 2006). It remains to be shown whether any of the three tomato members of CO family group Ia plays a role in tomato flowering, since the results reported so far do not exclude this possibility (Ben-Naim et al., 2006). Whether other CO family genes from potato have a stronger effect on flowering is st ...
MicroarraysExp
... If none of the columns are in U (so that the corresponding coefficients are 0), then, if we do any statistical test for b=0 and reject for p-value10000, we will make a lot of
mistak ...
... If none of the columns are in U (so that the corresponding coefficients are 0), then, if we do any statistical test for b=0 and reject for p-value
RNA interference
RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Historically, it was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon. Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998.Two types of small ribonucleic acid (RNA) molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to other specific messenger RNA (mRNA) molecules and either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons. It also influences development.The RNAi pathway is found in many eukaryotes, including animals, and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double-stranded fragments of ~20 nucleotide siRNAs. Each siRNA is unwound into two single-stranded RNAs (ssRNAs), the passenger strand and the guide strand. The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some organisms, this process spreads systemically, despite the initially limited molar concentrations of siRNA.RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.