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Gene Finding
Gene Finding

... Splicing: the removal of the introns. Performed by complexes called spliceosomes, containing both proteins and snRNA. The snRNA recognizes the splice sites through RNA-RNA base-pairing Recognition must be precise: a 1nt error can shift the reading frame making nonsense of its ...
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... or altered activities that can be useful to organisms in different or changing environments.  Plant and animal breeders often take advantage of such beneficial mutations.  The condition in which an organism has extra sets of chromosomes is called polyploidy.  Often larger and stronger than diploi ...
TOPIC 16: REGULATION OF GENE EXPRESSION
TOPIC 16: REGULATION OF GENE EXPRESSION

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... T antigen at all! The expected deletion of an interior segment of the T antigen does not occur. If these antigen proteins reflect their genes, this doesn’t make any sense at all, as both t and T are read from the same nucleotides in the same reading frame (see chapter 13). How can it be a deletion a ...
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Protein Synthesis DNA vs. RNA

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RNA interference



RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Historically, it was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon. Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998.Two types of small ribonucleic acid (RNA) molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to other specific messenger RNA (mRNA) molecules and either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons. It also influences development.The RNAi pathway is found in many eukaryotes, including animals, and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double-stranded fragments of ~20 nucleotide siRNAs. Each siRNA is unwound into two single-stranded RNAs (ssRNAs), the passenger strand and the guide strand. The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some organisms, this process spreads systemically, despite the initially limited molar concentrations of siRNA.RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.
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