a role for mitochondrial enzymes in inherited neoplasia and beyond
... The diagram shows the five complexes that are involved in the mitochondrial electron-transport chain. Complexes I–IV are the electron-transport complexes, whereas complex V synthesizes adenosine triphosphate (ATP). Electrons are passed down the four complexes (black arrows) to molecular oxygen and t ...
... The diagram shows the five complexes that are involved in the mitochondrial electron-transport chain. Complexes I–IV are the electron-transport complexes, whereas complex V synthesizes adenosine triphosphate (ATP). Electrons are passed down the four complexes (black arrows) to molecular oxygen and t ...
Sex Chromosome Dosage Effects On Gene Expression In
... reverse transcription polymerase chain reaction (qPCR) measures of expression for genes of interest identified in our core sample (Table S1, Material and Methods). To first verify our study design as a tool for probing SCD effects on gene expression, and to identify core SCD-sensitive genes, we scre ...
... reverse transcription polymerase chain reaction (qPCR) measures of expression for genes of interest identified in our core sample (Table S1, Material and Methods). To first verify our study design as a tool for probing SCD effects on gene expression, and to identify core SCD-sensitive genes, we scre ...
Characterization of Chicken MMP13 Expression and Genetic Effect
... follicles of sexually immature chicken ovaries, in the theca cell layers of all sized follicles of sexually mature chicken ovaries. Furthermore, we identified a positive element (positions –1863 to –1036) controlling chicken MMP13 transcription, and, in this region, six single nucleotide polymorphism ...
... follicles of sexually immature chicken ovaries, in the theca cell layers of all sized follicles of sexually mature chicken ovaries. Furthermore, we identified a positive element (positions –1863 to –1036) controlling chicken MMP13 transcription, and, in this region, six single nucleotide polymorphism ...
Mutations in SIN4 and RGR1 Cause Constitutive Expression of MAL
... MAL61promoter-lacZ reporter using the standard -galactosidase plate assay and MAL12 expression by assaying maltase activity levels in galactose-grown cells. Cloning of wild-type alleles of a mutant gene in strains CM-31 and CM-33: Constitutive mutants CM-31 and CM-33 were chosen as representatives ...
... MAL61promoter-lacZ reporter using the standard -galactosidase plate assay and MAL12 expression by assaying maltase activity levels in galactose-grown cells. Cloning of wild-type alleles of a mutant gene in strains CM-31 and CM-33: Constitutive mutants CM-31 and CM-33 were chosen as representatives ...
Prediction and investigation of novel proteins in DNA double
... Ottawa-Carleton Institute of Biology Carleton University Ottawa, Ontario ...
... Ottawa-Carleton Institute of Biology Carleton University Ottawa, Ontario ...
The Cutting Edge of Lung Cancer Research
... metastatic cancers. There are many genetic changes in each lung cancer. A conservative estimate is that a clinically obvious primary lung cancer can have 10-20 such changes. If preneoplastic lesions with a few but not all changes can be identified this rnay provide very early molecular diagnosis and ...
... metastatic cancers. There are many genetic changes in each lung cancer. A conservative estimate is that a clinically obvious primary lung cancer can have 10-20 such changes. If preneoplastic lesions with a few but not all changes can be identified this rnay provide very early molecular diagnosis and ...
PKB - Friedrich Miescher Institute for Biomedical Research
... Mutations of PI3K pathway genes in colorectal cancer Amino acid changes or amplifications observed for each gene in 146 colorectal cancers. When two mutations in the same gene in a tumor were observed, the mutations are separated by a slash. "Amp" indicates amplification, "wt" indicates wild-type s ...
... Mutations of PI3K pathway genes in colorectal cancer Amino acid changes or amplifications observed for each gene in 146 colorectal cancers. When two mutations in the same gene in a tumor were observed, the mutations are separated by a slash. "Amp" indicates amplification, "wt" indicates wild-type s ...
Physiological characterization of natural transformation in
... construct into the recipient chromosome can only occur via a Campbell-like mechanism. Interestingly, transformation frequencies obtained with pAVA2 13-41 (Table 2) were about 50-100 times lower than with pAVA2 13-8. This indicates that replacement recombination is more efficient than Campbell-like i ...
... construct into the recipient chromosome can only occur via a Campbell-like mechanism. Interestingly, transformation frequencies obtained with pAVA2 13-41 (Table 2) were about 50-100 times lower than with pAVA2 13-8. This indicates that replacement recombination is more efficient than Campbell-like i ...
Interaction of a GATA factor with cis-acting elements involved in light
... motifs, indeed existed in promoters of many, if not all, light-regulated genes. These proposed light responsive elements include the I box, GAF sites, and CGF sites in the promoters of RBCS and LHCB genes from pea and Arabidopsis [3,5,8,9]. However, the majority of the putative LREs have not been fu ...
... motifs, indeed existed in promoters of many, if not all, light-regulated genes. These proposed light responsive elements include the I box, GAF sites, and CGF sites in the promoters of RBCS and LHCB genes from pea and Arabidopsis [3,5,8,9]. However, the majority of the putative LREs have not been fu ...
The Differential Killing of Genes by Inversions in Prokaryotic Genomes
... determines the described location of a gene. If the sense strand is located on the leading strand, it is assumed that “the gene lies on the leading strand.” In prokaryotic genomes, the leading and lagging roles of DNA strands are predetermined by location of the origin of replication and the terminu ...
... determines the described location of a gene. If the sense strand is located on the leading strand, it is assumed that “the gene lies on the leading strand.” In prokaryotic genomes, the leading and lagging roles of DNA strands are predetermined by location of the origin of replication and the terminu ...
Molecular genetics of Rett syndrome and clinical
... mechanism that might account for this is dif®cult to predict. These genes may be neuronal-speci®c or widely expressed but detrimental only to neuronal development when misexpressed. In support of the idea that MeCP2 might regulate only a subset of genes, the CpG islands associated with the promoters ...
... mechanism that might account for this is dif®cult to predict. These genes may be neuronal-speci®c or widely expressed but detrimental only to neuronal development when misexpressed. In support of the idea that MeCP2 might regulate only a subset of genes, the CpG islands associated with the promoters ...
Full Text - Harvard University
... Phenotype. Genotype refers to the all the genetic information that constitutes an organism. Phenotype refers to all the observable traits or characteristics of that organism. Considering gene expression as an observable trait, the main focus of all the reverse engineering methods is to model the com ...
... Phenotype. Genotype refers to the all the genetic information that constitutes an organism. Phenotype refers to all the observable traits or characteristics of that organism. Considering gene expression as an observable trait, the main focus of all the reverse engineering methods is to model the com ...
Nucleotide Sequence of the Osmoregulatory proU Operon of
... and 1 kb of Mu c DNA (thick line); the BglII site was lost in the process of construction of pHYD58 and is therefore shown within parentheses. The sequence to the right of the EcoRV site, marked at approximately 0.6 kb in the figure, was determined. An ordered, overlapping series of deletions starti ...
... and 1 kb of Mu c DNA (thick line); the BglII site was lost in the process of construction of pHYD58 and is therefore shown within parentheses. The sequence to the right of the EcoRV site, marked at approximately 0.6 kb in the figure, was determined. An ordered, overlapping series of deletions starti ...
AnsteadSeniorHonorsThesis
... of that range is entered into Flybase in order to generate the Gbrowser displaying the genes specific to that region. Each gene is then sorted through, and genes of interest are identified by their molecular function and biological process performed. Genes associated with functions such as: DNA bind ...
... of that range is entered into Flybase in order to generate the Gbrowser displaying the genes specific to that region. Each gene is then sorted through, and genes of interest are identified by their molecular function and biological process performed. Genes associated with functions such as: DNA bind ...
Pseudogenes as regulators of biological function
... Regulation of mRNA stability The regulation of mRNA stability is one way in which gene expression can be controlled. The stability of an mRNA can be influenced by protein factors that bind at different locations in the RNA [38]. If a pseudogene has a high homology with the parent coding gene, includ ...
... Regulation of mRNA stability The regulation of mRNA stability is one way in which gene expression can be controlled. The stability of an mRNA can be influenced by protein factors that bind at different locations in the RNA [38]. If a pseudogene has a high homology with the parent coding gene, includ ...
Volume 16 - Number 1 January 2012 Atlas of Genetics and Cytogenetics
... Expressions of different members of the ADAM family have been investigated in breast cancer. Despite that some ADAM family members present differential expression between non neoplastic and breast cancer tissue, no difference was observed for ADAM10 (Lendeckel et al., 2005). Nevertheless, Liu and co ...
... Expressions of different members of the ADAM family have been investigated in breast cancer. Despite that some ADAM family members present differential expression between non neoplastic and breast cancer tissue, no difference was observed for ADAM10 (Lendeckel et al., 2005). Nevertheless, Liu and co ...
Of mice and (wo)men: genotype–phenotype
... Since the p53-binding domain is retained in Brca11700T, p53 may still bind to the mutant protein and, in the absence of the Brca1 C-terminal co-activation domain, become sequestered in an inactive complex. Lethality of Brca1D11/D11 embryos occur at 12.5–18.5 d.p.c. (19,22), thus showing that the Brc ...
... Since the p53-binding domain is retained in Brca11700T, p53 may still bind to the mutant protein and, in the absence of the Brca1 C-terminal co-activation domain, become sequestered in an inactive complex. Lethality of Brca1D11/D11 embryos occur at 12.5–18.5 d.p.c. (19,22), thus showing that the Brc ...
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... domains. The N-terminal domain (Sir3N) increases both the frequency and extent of telomere-proximal silencing when expressed ectopically in SIR1 yeast strains, although we are unable to detect interaction between this domain and any known components of the silencing machinery. In contrast to its eff ...
... domains. The N-terminal domain (Sir3N) increases both the frequency and extent of telomere-proximal silencing when expressed ectopically in SIR1 yeast strains, although we are unable to detect interaction between this domain and any known components of the silencing machinery. In contrast to its eff ...
Somatic Cell Gene Mutations in Humans
... usually nonselectable, and, when they do arise, may alter cell proliferation characteristics such that their primary frequencies become difficult to measure. For investigations of the mutation process itself and for monitoring human exposures to environmental mutagens, recourse is made to somatic mu ...
... usually nonselectable, and, when they do arise, may alter cell proliferation characteristics such that their primary frequencies become difficult to measure. For investigations of the mutation process itself and for monitoring human exposures to environmental mutagens, recourse is made to somatic mu ...
Slide 1
... Harmful and Helpful Mutations The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. ...
... Harmful and Helpful Mutations The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. ...
Pathogen Response Genes Mediate Caenorhabditis elegans Innate
... found to be upregulated in wild type worms but were not upregulated in fshr-1 mutants, suggesting that these genes are induced by FSHR-1 in worms infected with Pseudomonas and are thus targets of the FSHR-1 pathway. Through the use of quantitative real-time polymerase chain reactions (qRT-PCR), Powe ...
... found to be upregulated in wild type worms but were not upregulated in fshr-1 mutants, suggesting that these genes are induced by FSHR-1 in worms infected with Pseudomonas and are thus targets of the FSHR-1 pathway. Through the use of quantitative real-time polymerase chain reactions (qRT-PCR), Powe ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.