Lack of biological significance in the `linguistic features` of
... of ‘typographical errors’. As a result of these investigations, it was claimed (2) that, in clear contrast with protein coding DNA segments, the noncoding DNA parts are related with a considerable amount of redundancy. Very recently, however, these findings and/or claims have been strongly criticise ...
... of ‘typographical errors’. As a result of these investigations, it was claimed (2) that, in clear contrast with protein coding DNA segments, the noncoding DNA parts are related with a considerable amount of redundancy. Very recently, however, these findings and/or claims have been strongly criticise ...
Identical mutations in RAG1 or RAG2 genes leading
... The diversity of immunoglobulins and T-cell receptors (TCRs) is mediated by the somatic recombination of genes encoding variable (V), diversity (D), and joining (J) segments by a mechanism known as V(D)J recombination.1 The RAG1 and RAG2 proteins, the expression of which is restricted to immature ly ...
... The diversity of immunoglobulins and T-cell receptors (TCRs) is mediated by the somatic recombination of genes encoding variable (V), diversity (D), and joining (J) segments by a mechanism known as V(D)J recombination.1 The RAG1 and RAG2 proteins, the expression of which is restricted to immature ly ...
1st set of Journal Clubs this Wednesday!
... •lacY encodes galactoside permease which is required for transport of lactose into the cells. •lacA encodes galactoside transacetylase which transfers acetyl groups from acetyl-CoA to β-galactosides. The biological function of the enzymatic activity is unclear. ...
... •lacY encodes galactoside permease which is required for transport of lactose into the cells. •lacA encodes galactoside transacetylase which transfers acetyl groups from acetyl-CoA to β-galactosides. The biological function of the enzymatic activity is unclear. ...
Role of the p21 Protein in the Fanconi Anemia Pathway
... – Benign and malignant liver, brain and renal tumors ...
... – Benign and malignant liver, brain and renal tumors ...
Review Molecular Biology in Arteriosclerosis Research
... The topics discussed In this article illustrate how molecular biology will have a dramatic Impact on arteriosclerosis research. DNA clones for a small number of relevant proteins have been isolated, and studies are underway in numerous laboratories to extend these initial studies. The techniques of ...
... The topics discussed In this article illustrate how molecular biology will have a dramatic Impact on arteriosclerosis research. DNA clones for a small number of relevant proteins have been isolated, and studies are underway in numerous laboratories to extend these initial studies. The techniques of ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
... (other than the suspect) contributed to the evidence. These two LR hypotheses (either the suspect contributed or he did not) are exhaustive and mutually exclusive. Statisticians and computers often contrast two hypotheses, using (what is for them) straightforward mathematics. The LR here is a ratio ...
... (other than the suspect) contributed to the evidence. These two LR hypotheses (either the suspect contributed or he did not) are exhaustive and mutually exclusive. Statisticians and computers often contrast two hypotheses, using (what is for them) straightforward mathematics. The LR here is a ratio ...
BBSI Final Report
... electroporated into RN4220, (a SaPI1 negative strain of S. aureus). Upon infection with 80, it was shown that this region caused a significant reduction in 80alpha infectivity, indicating interference. Further studies will confirm the capsid size is indeed being reduced, causing the interference, a ...
... electroporated into RN4220, (a SaPI1 negative strain of S. aureus). Upon infection with 80, it was shown that this region caused a significant reduction in 80alpha infectivity, indicating interference. Further studies will confirm the capsid size is indeed being reduced, causing the interference, a ...
Explaining the Likelihood Ratio in DNA Mixture
... (other than the suspect) contributed to the evidence. These two LR hypotheses (either the suspect contributed or he did not) are exhaustive and mutually exclusive. Statisticians and computers often contrast two hypotheses, using (what is for them) straightforward mathematics. The LR here is a ratio ...
... (other than the suspect) contributed to the evidence. These two LR hypotheses (either the suspect contributed or he did not) are exhaustive and mutually exclusive. Statisticians and computers often contrast two hypotheses, using (what is for them) straightforward mathematics. The LR here is a ratio ...
The Development of a Genetic Marker for Resistance to Fescue
... sequence which may serve as a marker for susceptibility to Fescue Toxicosis. The sequence variation was an intronic A→G single nucleotide polymorphism (SNP) that was found in the region of the DRD2 gene, creating the genotypes AA, AG and GG. In this study, the informativeness of this genetic marker ...
... sequence which may serve as a marker for susceptibility to Fescue Toxicosis. The sequence variation was an intronic A→G single nucleotide polymorphism (SNP) that was found in the region of the DRD2 gene, creating the genotypes AA, AG and GG. In this study, the informativeness of this genetic marker ...
Identifying 3D expression domains by graph clustering
... Before the publication of the TRIP method this generally required low throughput analyses as reporters could only target specific genomic loci. The process of mapping the integration sites is extremely laborious and not suitable for genomewide interrogation[22]. TRIP solves these problems by usi ...
... Before the publication of the TRIP method this generally required low throughput analyses as reporters could only target specific genomic loci. The process of mapping the integration sites is extremely laborious and not suitable for genomewide interrogation[22]. TRIP solves these problems by usi ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
... mitotic recombination between closely linked markers. This result was reproduced in the mitotic crosses in Table 3, where factors of increase of more than tenfold relative to the spontaneous rate were found. However, the stimulation of recombination between plasmid and chromosome by the same doses o ...
... mitotic recombination between closely linked markers. This result was reproduced in the mitotic crosses in Table 3, where factors of increase of more than tenfold relative to the spontaneous rate were found. However, the stimulation of recombination between plasmid and chromosome by the same doses o ...
De novo mutations in familial adenomatous polyposis (FAP)
... mitotic divisions with the number rising every year.25 If the germline mutations arose during these premeiotic mitotic divisions one would expect a marked bias for a paternal origin and a striking parental age effect as seen in disorders like Apert syndrome12 and multiple endocrine neoplasia type 2B ...
... mitotic divisions with the number rising every year.25 If the germline mutations arose during these premeiotic mitotic divisions one would expect a marked bias for a paternal origin and a striking parental age effect as seen in disorders like Apert syndrome12 and multiple endocrine neoplasia type 2B ...
Molecular markers in Brassica Rapa
... of BrCRTISO2 in a normal yellow-colored (YE) cultivar, but full-length BrCRTISO1 transcripts were not detected in the OC cultivar. Genomic sequence analysis revealed that BrCRTISO1 of the OC cultivar had many sequence variations, including single nucleotide polymorphisms (SNPs) and insertions and de ...
... of BrCRTISO2 in a normal yellow-colored (YE) cultivar, but full-length BrCRTISO1 transcripts were not detected in the OC cultivar. Genomic sequence analysis revealed that BrCRTISO1 of the OC cultivar had many sequence variations, including single nucleotide polymorphisms (SNPs) and insertions and de ...
ATM and Artemis promote homologous recombination of
... Homologous recombination (HR) and non-homologous end joining (NHEJ) represent two conceptually different pathways for repairing DNA double-strand breaks (DSBs), the lesion at the heart of many physiological and pathophysiological processes in mammalian cells. HR uses homologous sequences on the sist ...
... Homologous recombination (HR) and non-homologous end joining (NHEJ) represent two conceptually different pathways for repairing DNA double-strand breaks (DSBs), the lesion at the heart of many physiological and pathophysiological processes in mammalian cells. HR uses homologous sequences on the sist ...
1Department of Hematology, 2Department of Dermatology, Leiden
... bone marrow samples from treatment naïve B-ALL, chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), and hairy cell leukemia (HCL) patients and healthy donors by flow cytometry with a fluorescently labeled GPI-specific aerolysin FLAER and CD52 and CD19 ...
... bone marrow samples from treatment naïve B-ALL, chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), and hairy cell leukemia (HCL) patients and healthy donors by flow cytometry with a fluorescently labeled GPI-specific aerolysin FLAER and CD52 and CD19 ...
Screening Mutant Libraries of Fungal Laccases in the Presence of
... Next to Escherichia coli, S. cerevisiae is the most commonly used host organism in laboratory evolution. Its sophisticated cellular machinery simplifies mutant library generation. By homologous recombination, otherwise cumbersome ligation of mutant genes into expression vectors or shuffling of error ...
... Next to Escherichia coli, S. cerevisiae is the most commonly used host organism in laboratory evolution. Its sophisticated cellular machinery simplifies mutant library generation. By homologous recombination, otherwise cumbersome ligation of mutant genes into expression vectors or shuffling of error ...
Comparison of two codon optimization strategies to enhance
... Many transgenic proteins expressed in E. coli are recovered as insoluble aggregates in the form of inclusion bodies. The formation of these aggregates seems to be independent of the type of protein, and this drawback has been proven difficult to overcome [18, 19]. Nevertheless, the fact that inclusi ...
... Many transgenic proteins expressed in E. coli are recovered as insoluble aggregates in the form of inclusion bodies. The formation of these aggregates seems to be independent of the type of protein, and this drawback has been proven difficult to overcome [18, 19]. Nevertheless, the fact that inclusi ...
Repeat mediated gene duplication in the Drosophila
... was added, and this longer sequence was used to identify the endpoints (via BLAST). This process was repeated until the alignment no longer terminated at the end of the partition, and the duplication endpoint was identified. Protein coding sequences were inferred using the initial published annotatio ...
... was added, and this longer sequence was used to identify the endpoints (via BLAST). This process was repeated until the alignment no longer terminated at the end of the partition, and the duplication endpoint was identified. Protein coding sequences were inferred using the initial published annotatio ...
Chromosomal breakpoint positions suggest a direct role for radiation
... properties, but was not sucient for transformation (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause pe ...
... properties, but was not sucient for transformation (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause pe ...
Evolution of synonymous codon usage in metazoans Laurent Duret
... the size of populations that can be studied in laboratories, this experimental approach is limited to the identification of mutations with a strong phenotypic impact [1]. This problem is far from negligible; for example, 50% of gene knockouts in yeast have no detectable effect on the phenotype [2]. ...
... the size of populations that can be studied in laboratories, this experimental approach is limited to the identification of mutations with a strong phenotypic impact [1]. This problem is far from negligible; for example, 50% of gene knockouts in yeast have no detectable effect on the phenotype [2]. ...
Gene transfer in bacteria - McGraw Hill Higher Education
... Most are crucial to maintenance of earth's environment • Release oxygen to atmosphere • Recycle carbon, nitrogen, and other elements • Digest human and other animal waste • Neutralize pesticides and other pollutants • Produce vitamins and other materials essential to humans and other organisms Copyr ...
... Most are crucial to maintenance of earth's environment • Release oxygen to atmosphere • Recycle carbon, nitrogen, and other elements • Digest human and other animal waste • Neutralize pesticides and other pollutants • Produce vitamins and other materials essential to humans and other organisms Copyr ...
Imprinted gene expression in hybrids: perturbed
... The developmental establishment and maintenance of imprinting involve complex interactions between cis-regulatory elements and trans-acting factors, some of which are themselves subject to imprinting (Kelsey and Feil, 2013; Wolf, 2013). The transcription factor gene ZAC1 is imprinted in both mice an ...
... The developmental establishment and maintenance of imprinting involve complex interactions between cis-regulatory elements and trans-acting factors, some of which are themselves subject to imprinting (Kelsey and Feil, 2013; Wolf, 2013). The transcription factor gene ZAC1 is imprinted in both mice an ...
Growth medium composition-determined regulatory mechanisms
... Expression of the phenol degradation pathway in Pseudomonas putida strain PaW85 requires coordinated transcription of the plasmid-borne pheBA operon encoding catechol 1,2-dioxygenase and phenol monooxygenase, respectively, and the chromosomally encoded catechol degradation catBCA operon. Transcripti ...
... Expression of the phenol degradation pathway in Pseudomonas putida strain PaW85 requires coordinated transcription of the plasmid-borne pheBA operon encoding catechol 1,2-dioxygenase and phenol monooxygenase, respectively, and the chromosomally encoded catechol degradation catBCA operon. Transcripti ...
LECTURE 13: EPIGENETICS – IMPRINTING Reading: Ch. 18, p
... pronucleus and a paternally-derived pronucleus developed normally, but embryos from the fusion of two maternally-derived pronuclei or two maternally-derived pronuclei did not develop normally. The only possible genetic difference between males and females in this experiment was the sex chromosomes, ...
... pronucleus and a paternally-derived pronucleus developed normally, but embryos from the fusion of two maternally-derived pronuclei or two maternally-derived pronuclei did not develop normally. The only possible genetic difference between males and females in this experiment was the sex chromosomes, ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.