- Lab Fabiana Perocchi
... Figure 1 Measuring allelic expression on a tiling array. (A) The array contains 25-mer probes (black and blue) that tile both strands of the genome of S288c with a probe offset of 8 bp and a 4-bp shift between the two strands. The array also contains probes (red) complementary to the YJM789 sequence ...
... Figure 1 Measuring allelic expression on a tiling array. (A) The array contains 25-mer probes (black and blue) that tile both strands of the genome of S288c with a probe offset of 8 bp and a 4-bp shift between the two strands. The array also contains probes (red) complementary to the YJM789 sequence ...
Control of the acetamidase gene of Mycobacterium smegmatis by
... AmiC and AmiD also have a role to play in AmiE expression. We constructed individual deletion mutants of amiC and amiD and looked at acetamidase expression under non-induced and induced conditions. Fig. 1 shows the deletion constructs used to generate the mutants by homologous recombination. Mutants ...
... AmiC and AmiD also have a role to play in AmiE expression. We constructed individual deletion mutants of amiC and amiD and looked at acetamidase expression under non-induced and induced conditions. Fig. 1 shows the deletion constructs used to generate the mutants by homologous recombination. Mutants ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... islands upstream of genes for which an altered methylation status in one or more types of tumours has been reported in literature. In case interesting results are obtained by users, it is possible to develop methylation kits specific for a certain tumour in collaboration with MRC-Holland. Interpreta ...
... islands upstream of genes for which an altered methylation status in one or more types of tumours has been reported in literature. In case interesting results are obtained by users, it is possible to develop methylation kits specific for a certain tumour in collaboration with MRC-Holland. Interpreta ...
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY
... (1) a and c only (2) a and b only (3) b only (4) b and c only Ans (2) 49. Offsprings formed during sexual reproduction exhibits more variation than, those formed asexual method, because, (1) sexual reproduction is more complicated (2) genetic material comes from two different individuals (3) genetic ...
... (1) a and c only (2) a and b only (3) b only (4) b and c only Ans (2) 49. Offsprings formed during sexual reproduction exhibits more variation than, those formed asexual method, because, (1) sexual reproduction is more complicated (2) genetic material comes from two different individuals (3) genetic ...
P-Element Transformation with period Locus DNA Restores
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
Coupling of zygotic transcription to mitotic control at the Drosophila
... degradation are coupled through the transcription of specific microRNAs that promote maternal RNA deadenylation and clearance (Giraldez et al., 2006). A similar role for zygotically expressed miRNAs has been postulated for maternal RNA degradation during Drosophila MBT (Bushati et al., 2008). Howeve ...
... degradation are coupled through the transcription of specific microRNAs that promote maternal RNA deadenylation and clearance (Giraldez et al., 2006). A similar role for zygotically expressed miRNAs has been postulated for maternal RNA degradation during Drosophila MBT (Bushati et al., 2008). Howeve ...
annotation and analysis of newly discovered mycobacteriophage
... MEME to determine in either Dori or Firecracker contains repetitive DNA elements. We observed a highly repeated sequence in the Firecracker genome using Gepard, and using MEME we found that this repeat is a 17 base pair palindrome. The palindromic sequence contained a 3 nucleotide center region that ...
... MEME to determine in either Dori or Firecracker contains repetitive DNA elements. We observed a highly repeated sequence in the Firecracker genome using Gepard, and using MEME we found that this repeat is a 17 base pair palindrome. The palindromic sequence contained a 3 nucleotide center region that ...
gene duplication in the evolution of sexual dimorphism
... number of sex-biased genes identified. Furthermore, because evolutionarily recent gene duplicates are expected to be more similar in expression, minor differences in expression are more likely to be detected when sample size is large. Next, we used the fact that these two studies did not always agre ...
... number of sex-biased genes identified. Furthermore, because evolutionarily recent gene duplicates are expected to be more similar in expression, minor differences in expression are more likely to be detected when sample size is large. Next, we used the fact that these two studies did not always agre ...
Ovarian surface epithelium: family history and early events in
... mutations showed significantly larger nuclei and nonhomogenous chromatin distribution than in control ovaries. Similar nuclear atypia was also identified in the noncancerous surface epithelium adjacent to primary ovarian tumors compared to OSE from control ovaries [12]. However, there is no evidence ...
... mutations showed significantly larger nuclei and nonhomogenous chromatin distribution than in control ovaries. Similar nuclear atypia was also identified in the noncancerous surface epithelium adjacent to primary ovarian tumors compared to OSE from control ovaries [12]. However, there is no evidence ...
Illustrating Python via Bioinformatics Examples
... make the difference between the cells. This regulation of genes is orchestrated by an immensely complex mechanism, which we have only started to understand. A central part of this mechanism consists of molecules called transcription factors that float around in the cell and attach to DNA, and in doi ...
... make the difference between the cells. This regulation of genes is orchestrated by an immensely complex mechanism, which we have only started to understand. A central part of this mechanism consists of molecules called transcription factors that float around in the cell and attach to DNA, and in doi ...
The Structures of DNA and RNA
... the hereditary information within chromosomes, immediately focused attention on its structure. It was hoped that knowledge of the structure would reveal how DNA carries the genetic messages that are replicated when chromosomes divide to produce two identical copies of themselves. During the late 194 ...
... the hereditary information within chromosomes, immediately focused attention on its structure. It was hoped that knowledge of the structure would reveal how DNA carries the genetic messages that are replicated when chromosomes divide to produce two identical copies of themselves. During the late 194 ...
Journal of Plant Physiology
... to growth and development, as well as in responses to environmental stimuli (Riechmann and Meyerowitz, 1998; Gutterson and Reuber, 2004). The Arabidopsis genome encodes 145 AP2/EREBP proteins. Recently Mizoi et al. (2012) classified these proteins into five families, namely AP2, RAV, DREB (dehydration ...
... to growth and development, as well as in responses to environmental stimuli (Riechmann and Meyerowitz, 1998; Gutterson and Reuber, 2004). The Arabidopsis genome encodes 145 AP2/EREBP proteins. Recently Mizoi et al. (2012) classified these proteins into five families, namely AP2, RAV, DREB (dehydration ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
... single-stranded DNA, which stimulates a protease activity that is normally silent in the RecA protein. The RecA* protease activity cleaves the LexA protein. LexA is a transcriptional repressor of about 18 genes, many of which are involved in DNA repair and the inhibition of cell division. Therefore, ...
... single-stranded DNA, which stimulates a protease activity that is normally silent in the RecA protein. The RecA* protease activity cleaves the LexA protein. LexA is a transcriptional repressor of about 18 genes, many of which are involved in DNA repair and the inhibition of cell division. Therefore, ...
The Isolation of Mutagen-Sensitive nuv Mutants of
... thedeterminations were carriedout as two large experiments, one for sensitivity to M N N G and the other for sensitivity to 4 - N Q O , in which all the plates at a particular mutagen concentration were made up using the same mutagen-containing media. This was necessary due to the inherent instabili ...
... thedeterminations were carriedout as two large experiments, one for sensitivity to M N N G and the other for sensitivity to 4 - N Q O , in which all the plates at a particular mutagen concentration were made up using the same mutagen-containing media. This was necessary due to the inherent instabili ...
Induction of XIST expression from the human active
... a significantly different pattern with product observed in both the cytoplasmic and nuclear fractions (Fig. 3), as would be expected of a mRNA which is transported into the cytoplasm for translation. Induced XIST expression does not alter expression of other X-linked genes The induction of XIST expr ...
... a significantly different pattern with product observed in both the cytoplasmic and nuclear fractions (Fig. 3), as would be expected of a mRNA which is transported into the cytoplasm for translation. Induced XIST expression does not alter expression of other X-linked genes The induction of XIST expr ...
Rolling circle transcription on smallest size double stranded DNA
... While DNA is much more stable than its twin RNA owing to the absence of the latter’s ribose 2’ Hydroxyl group it is still very much affected by its surroundings. The major modes of damage to hydrolysed DNA are depurination of the sugar base, deamination of the base itself and general oxidative proce ...
... While DNA is much more stable than its twin RNA owing to the absence of the latter’s ribose 2’ Hydroxyl group it is still very much affected by its surroundings. The major modes of damage to hydrolysed DNA are depurination of the sugar base, deamination of the base itself and general oxidative proce ...
Imprinting capacity of gamete lineages in C. elegans
... with DNA methylation levels, with the maternally-derived alleles generally being more methylated than the paternally-derived alleles. Although reports of parent-of-origin effects in other organisms have been abundant, accounts of parent-of-origin phenomenon in C. elegans have been very rare. A scree ...
... with DNA methylation levels, with the maternally-derived alleles generally being more methylated than the paternally-derived alleles. Although reports of parent-of-origin effects in other organisms have been abundant, accounts of parent-of-origin phenomenon in C. elegans have been very rare. A scree ...
Creating conditional dual fluorescence labelled transgenic animals
... critical to the field. We developed a fast and straightforward method to determine ncRNAtransgene copy number, orientation, and insertion site in the genome. Furthermore, upon tissuespecific expression of ncRNA, a Cre-loxP mediated dual-fluorescence expression system facilitates fluorescence signal ...
... critical to the field. We developed a fast and straightforward method to determine ncRNAtransgene copy number, orientation, and insertion site in the genome. Furthermore, upon tissuespecific expression of ncRNA, a Cre-loxP mediated dual-fluorescence expression system facilitates fluorescence signal ...
Genetics - Michael
... traits, farmers were able to establish food crops that produced larger fruits (and seeds) and made harvesting easier. Civilizations that could produce more food could also sustain larger armies. The cultural influence of these civilizations expanded over time, and with their success spread their kno ...
... traits, farmers were able to establish food crops that produced larger fruits (and seeds) and made harvesting easier. Civilizations that could produce more food could also sustain larger armies. The cultural influence of these civilizations expanded over time, and with their success spread their kno ...
Gene Finding by Computational Analysis
... • DNA is usually tightly wound around histone proteins and forms a chromosome • The total info stored in all chromosomes constitutes a genome • In most multi-cell organisms, every cell contains the same complete set of chromosomes – May have some small different due to mutation ...
... • DNA is usually tightly wound around histone proteins and forms a chromosome • The total info stored in all chromosomes constitutes a genome • In most multi-cell organisms, every cell contains the same complete set of chromosomes – May have some small different due to mutation ...
PDF
... FIGURE 2 | Orientation of magnetic field and magnetic forces. DNA tethered magnetic beads experience a force (F) along the field gradient. (A) A pair of horizontally placed magnets induces a horizontal magnetic moment (µ) in the beads. Due to anisotropic polarization, the induced magnetic moment wil ...
... FIGURE 2 | Orientation of magnetic field and magnetic forces. DNA tethered magnetic beads experience a force (F) along the field gradient. (A) A pair of horizontally placed magnets induces a horizontal magnetic moment (µ) in the beads. Due to anisotropic polarization, the induced magnetic moment wil ...
The use of the twin model to investigate the genetics and
... occurs from the age of 40 years onwards. It has been observed clinically that the senescence of naevi appears to be delayed in patients with multiple atypical naevi, especially in those belonging to susceptible melanoma families. The individuals with multiple atypical naevi also appear to have delay ...
... occurs from the age of 40 years onwards. It has been observed clinically that the senescence of naevi appears to be delayed in patients with multiple atypical naevi, especially in those belonging to susceptible melanoma families. The individuals with multiple atypical naevi also appear to have delay ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.