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Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... short DNA sequence just downstream of the promoter near the beginning of lacZ called the lac operator Repressor bound to the operator interferes with binding of RNAP to the promoter, and therefore mRNA encoding LacZ and LacY is only made at very low levels In the presence of lactose, a lactose metab ...
2015 Test 3 study guide Bio 105
2015 Test 3 study guide Bio 105

... • How many nucleotides make up a codon • What does a codon code for? (amino acid) • What is needed for translation? (mRNA, rRNA, tRNA, amino acids, ribosomes) • 6.7 Translation part two • What is a anticodon and what kind of RNA contains the anticodon? • Initiation, elongation, and termination; star ...
Genética Molecular em Medicina Transfusional
Genética Molecular em Medicina Transfusional

... • Base calling programs predict nucleotide locations in sequencing reads where data anomalies occur. Such as multiple peaks at one nucleotide location, spread out peaks, low intensity peaks. ...
投影片 1
投影片 1

... Superhelix, 6 nucleosome per turn, supported by EM and X-ray studies ...
From DNA to Protein Name: What does DNA stand for? What is DNA
From DNA to Protein Name: What does DNA stand for? What is DNA

... what would the nucleotide sequence on the complementary strand of DNA? ...
Chapter 14 - Genomes and genomics
Chapter 14 - Genomes and genomics

... Norm Dovici – Capillary electrophoresis ...
Chromatin Structure 1
Chromatin Structure 1

... 2. Capable of genetic activity (transcription). Normal state of most DNA during interphase. 3. Euchromatin is often divided into several distinguishable states of folding (although tightness of folding is probably really continuous from relatively loose to relatively tight). Correlation between fold ...
Why-do-cells
Why-do-cells

... out. They need repair, and, just like an old car, eventually need ...
Chromosomes
Chromosomes

... • A single recognition site for the restriction enzyme AluI located near the middle of the Alu element. • Alu elements are found only in primates. • Human chromosomes contain about 1,000,000 Alu copies (10% of the total genome). • Alu is a "jumping gene" – a transposable DNA sequence that "reproduce ...
DNA Structure and Replication
DNA Structure and Replication

... • DNA molecule separates at its bases • Forms split, or replication fork • Each strand acts as a template ...
Gene expression An organism`s genome is the complete set of
Gene expression An organism`s genome is the complete set of

... An organism’s genome is the complete set of genes in each of its cells. Given an organism, every one of its cells has a copy of the exact same genome, but ◆ not all its cells express the same genes ◆ different genes express under different conditions Measure the levels of the various mRNAs in a cell ...
Topic 4: Genetics - wfs
Topic 4: Genetics - wfs

... medicines specifically engineered to an individuals needs. It may also give new insights into the origins, evolution and migrations of humans. 7. Because genetic code is universal, when genes are transferred between species, these genes will produce the same polypeptides. This occurs when the gene t ...
DNA, RNA, Genetic Engineering
DNA, RNA, Genetic Engineering

... Semiconservative (one original and one new strand) Copying done by DNA polymerase Okazaki fragments 3’ to 5’ (leading v. lagging strand) Mitosis and Meiosis ...
BILD 10.Problem Set 3 KEY
BILD 10.Problem Set 3 KEY

... a) cookie is to recipe. b) fish is to bicycle. c) cookie is to oven. d) oven is to cookie. e) recipe is to cookie. 9) During transcription, at the point where the DNA strand being copied has an adenine, _______________ is added to the mRNA. a) a cytosine b) an adenine c) a uracil d) a thymine e) a t ...
downloadable  file
downloadable file

... To start, you need a piece of DNA which you want to sequence. Next, you add a DNA priming sequence, the four nucleotides and an enzyme called DNA polymerase which incorporates new nucleotide bases making a new piece of DNA which is a copy of the original piece. In Sanger’s original method, four diff ...
A 3D pattern matching algorithm for DNA sequences
A 3D pattern matching algorithm for DNA sequences

... Biologists usually work with textual DNA sequences (A, C, G, T). Linear coding offers only a local and a onedimensional vision of the molecule. The 3D structure of DNA is known to be very important in many essential biological mechanisms. ...
Chapter 12 Review PPT
Chapter 12 Review PPT

... Identify the three main components in the nucleotide The circles are the phosphate group, the pentagons are deoxyribose, and the A and T (adenosine and thymine) are the bases. ...
point of view that is personal rather than scientific
point of view that is personal rather than scientific

... Identify the three main components in the nucleotide The circles are the phosphate group, the pentagons are deoxyribose, and the A and T (adenosine and thymine) are the bases. ...
PPT File
PPT File

... • An interesting model for studying the role of environmental factors in epigenetic modifications – Epigenetically concordant at birth in most cases – Differences (DNA methylation and histone modifications) accumulate with age – Greatest in those who had lived together for the smallest amount of tim ...
What is Genetic Engineering?
What is Genetic Engineering?

... DNA codes for the proteins that determine our traits. ...
Re-closing linearized plasmids
Re-closing linearized plasmids

... Identify correct clones by PCR or restriction digest. The appropriate screening method should distinguish between the desired plasmid and the parental plasmid. If using PCR, see the PCR protocols page for “Insert verification with Vent.” Analyze the PCR products or restriction digests on a 1% agaros ...
DNA quantification
DNA quantification

... DNA concentration and purity determination Why: •Calculate how much to use in reaction or on gel •Determine whether isolation was successful •Determine whether DNA is clean enough to use. DNA easily dissolves in aqueous solutions. However, at high concentrations (10 mg/ml and above), dissolved DNA i ...
1 - I`m Curious
1 - I`m Curious

... What is a Gene? Hint - Look at the navigation bar at the top, you'll need to click on "What is a Gene" to continue. 4. What is a gene? 5. What are genes made of? 6. How many genes do humans have? 7. What type of molecule do genes contain the instructions for building? 8. Blood cells use a protein ca ...
Document
Document

... the Nobel Prize in physiology or medicine for their work. ...


... 4. How is gene Z used as a marker? (1) 5. What is Bioreactor? What are the advantages of Stirred tank Bioreactor overShake flask. Show diagrammatically a simple Stirred tank Bioreactor? (1) 6. Name the genetically engineered human Insulin? (1) 7. Write the Scientific name of nematode that attacks th ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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