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...  Goals/uses of transformation & genetic engineering: o significance of plasmids, restriction enzymes & ligase, “sticky ends”  GMOs: production, uses, controversy  Animal cloning: process, controversy  DNA technology o PCR o Electrophoresis: How does it work? What can it be used for?  How is the ...
DNA Lab Techniques
DNA Lab Techniques

... of a group of genetically identical cells • May be produced by asexual reproduction (mitosis) ...
Slide 1 - Loyola Blakefield
Slide 1 - Loyola Blakefield

... Nucleotides and primers in solution initiate replication. ...
DNA, Chromosomes & Genes
DNA, Chromosomes & Genes

... What is a GENE? • A specific sequence of bases – Sequences carry the information needed for constructing proteins • Proteins provide the structural components of cells and tissues as well as enzymes for essential biochemical reactions. ...
DNA and proteins
DNA and proteins

... • A genome is the entire sequence of DNA of an organism (about 25000 genes in the human genome). • Each gene occupies a specific locus (position) on a chromosome and each chromosome consists of one molecule of DNA. • The DNA is wrapped around basic histone proteins (Chromatin) • In between genes is ...
DNA, Chromosomes & Genes - Science
DNA, Chromosomes & Genes - Science

... • A specific sequence of bases – Sequences carry the information needed for constructing proteins • Proteins provide the structural components of cells and tissues as well as enzymes for essential biochemical reactions. ...
Biotechnology webquest
Biotechnology webquest

... together. (Include nitrogen bases and which type of enzyme is used at each stage.) a) b) c) ...
From Mendel to DNA
From Mendel to DNA

... 3. The development of the microscope played an important part in helping to convince people that Mendel was right. How? 4. Explain with reference to the structure of DNA the saying ‘One gene, one protein’. ...
How DNA Determines Traits - Liberty Union High School District
How DNA Determines Traits - Liberty Union High School District

... Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with 6 genes on it. You job is to analyze the genes of its DNA and determine what traits the organism has. ...
Midterm Review Paper
Midterm Review Paper

... 1. How many chromosomes are in a “normal” human karyotype? 2. How would Down’s syndrome be detected on a karyotype? 3. Know how to read the genetic code chart (both circle and square). 4. What is the difference between a point mutation and a chromosomal mutation? 5. What is produced during transcrip ...
1. Compare the organization of prokaryotic and eukaryotic genomes.
1. Compare the organization of prokaryotic and eukaryotic genomes.

... methylation, genomic imprinting, and histone acetylation. • DNA methylation  the attachment of methyl groups (-CH3) to DNA bases -Inactive DNA is usually highly methylated (adding methyl groups inactivates DNA) • Genomic imprinting  where methylation permanently turns off either the maternal or pa ...
1. Compare the organization of prokaryotic and
1. Compare the organization of prokaryotic and

... methylation, genomic imprinting, and histone acetylation. • DNA methylation  the attachment of methyl groups (-CH3) to DNA bases -Inactive DNA is usually highly methylated (adding methyl groups inactivates DNA) • Genomic imprinting  where methylation permanently turns off either the maternal or pa ...
DNA Structure: Deoxyribonucleic acid
DNA Structure: Deoxyribonucleic acid

... _____________________________________________________________ Inherited example: _____________________________________________ Why is this trait an inherited trait? ________________________________ _____________________________________________________________ Definition of Environmental Trait: _____ ...
Genetic Engineering and Recombinant DNA
Genetic Engineering and Recombinant DNA

... Restriction Enzymes • Bacteria produce many different restriction enzymes that cut genetic material at different sites. Some make “blunt” ends: ATTC GGATC TAAG CCTAG Some make “sticky” ends: ATTCGG ATC TAA GCCTAG These pieces are restriction fragments. ...
History of Genetics
History of Genetics

... • 1966: Marshall Nirenberg solves the genetic code, showing that 3 DNA bases code for one amino acid. ...
Genetic Engineering
Genetic Engineering

... Recognize some of the basic strategies and methods of gene manipulation and analysis.  Identify representative examples of the applications of DNA technology.  Be prepared to discuss the implications of ...
Teacher Resource 8: Genetic engineering
Teacher Resource 8: Genetic engineering

... Learners work in groups of 2 or 3. Each has a worksheet that they can cut out the statements explaining the process of genetically engineering insulin, including the use of antibiotic marker genes to select plasmids containing the insulin gene. Learners work together to place the statements in the c ...
DNA Technology
DNA Technology

... EE ...
You should be able to find the information necessary to answer
You should be able to find the information necessary to answer

... 17. Explain why it is important for a bacterial cell to be able to turn off the synthesis of gene products if those products are already available in its environment, or to turn on synthesis of a substance it needs only under some circumstances. ...
No Slide Title
No Slide Title

... • Proteins: macromolecules composed of one or more chains of amino acids • Amino acids: class of 20 different organic compounds containing a basic amino group (NH2) and an acidic carboxyl group (-COOH) • The order of amino acids is determined by the base sequence of nucleotides in the gene coding fo ...
problem set
problem set

... perform different functions in a given organism. Orthologous genes typically perform the same function in different organisms, and have diverged in sequence due to mutations associated with speciation (Fig. 6.26b). The complexity of an organism is not simply related to the size of its genome. Due to ...
review-genetics-final-exam-2016
review-genetics-final-exam-2016

... 50. What are restriction enzymes used for? 51. What process is used to separate the DNA fragments after restriction enzymes have been used? 52. If an electrophoresis gel was used to separate DNA fragments and it ran from bottom to top, where would the longer fragments be located? 53. What charge doe ...
Molecular Biology
Molecular Biology

... How do we know that DNA is the molecule that transfers info? • T.H. Morgan showed that differences in chromosomes determined fly traits ...
09/06
09/06

... Molecular Basis for Relationship between Genotype and Phenotype ...
Insects and genetics
Insects and genetics

... 5. Mendel's law of _____________ states that alternative forms of a particular factor (gene) remain discrete during the reproductive process; his second law, the law of ____________ ____________, states that different factors are inherited independently of one another. 6. Who was Thomas Hunt Morgan? ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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