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Genetic Exchange - Pennsylvania State University
Genetic Exchange - Pennsylvania State University

... Self-replicative recombination •Transposon or IS self-replicates copy to splice into DNA at a specific target sequences. • Endonuclease activity cuts target sequence, leaving single strand overhanging ends. •Transposon is ligated to ends. • Gaps are filled by DNA polymerase to yield a target sequen ...
Genetics Exam 3
Genetics Exam 3

... cell types. ________________________________ A chromosomal mutation in which there is a change in position of chromosome segments to a different location in the genome. ________________________________ A gene present in only one dose. ________________________________ An enzyme that introduces or eli ...
Regulation of Gene Expression Outline Objectives are first and
Regulation of Gene Expression Outline Objectives are first and

... i) chromatin structure -DNA organized into nucleosomes: about 200 b.p. wrapped around a protein core -pr core= 8 histones *Histones are basic, contain positively charged amino acids, which are attracted to and bind with the negative charges of DNA (from the backbone phosphate groups) -DNA tightly wr ...
Introduction to Next Generation Sequencing
Introduction to Next Generation Sequencing

... • MAQ • RMAP • MOSAIK • BLAST • ELAND (Illumina) • Determine the target genome sequence (i.e., repeat classes) • Mapping options • Number of allowed mis-matches (as function of position) • Number of mapped loci (e.g., 1 = unique read sequence) • Generate Consensus Sequence and identify SNPs • Genera ...
DNA Notes Part 1
DNA Notes Part 1

... found when a cell is dividing! - Hold all genetic information. - Chromosomes are passed on to an offspring by its parents. Examples: Humans = 46 Shrimp = 254 Chimps = 48 Chicken = 78 Gorilla = 48 Wolf ...
SNC2D Genes - Malvern Science
SNC2D Genes - Malvern Science

... • Humans have 46 chromosomes in each somatic (body) cell (except the gametes = ovum/sperm, which have 23) • Different species may have different numbers of chromosomes ...
Mapping the DNA Damage Response
Mapping the DNA Damage Response

... Response Case study reveals transcription factor (TF) modules, dynamic TF binding and an expanded role for cell cycle regulators ...
Microbiology Exam II - University of Evansville Faculty Web sites
Microbiology Exam II - University of Evansville Faculty Web sites

... Multiple Choice: Circle the most correct answer for each of the following questions. (2 points each) 1. In a DNA molecule, which base would you expect to find paired with cytosine? a. guanine b. uracil c. adenine d. thymine e. cytosine 2. DNA serves as the template to initiate protein synthesis. Wha ...
Genetics Study Guide
Genetics Study Guide

... 11. A string of nucleotides that has instructions for a certain trait is a gene. 12. The diagram used to trace a trait through generations of a family is a pedigree. 13. What does each gene have instructions for making? A protein 14. When a plant fertilizes itself, it is called self-pollinating plan ...
GM skills - KingsfieldBiology
GM skills - KingsfieldBiology

... Not that different from selective breeding but less inbreeding necessary Genetic “mistakes” are made on purpose Is this paving the way to eugenics, selectively breeding humans ...
DNA_Project - Berkeley Cosmology Group
DNA_Project - Berkeley Cosmology Group

... We did all about DNA. So to start off: DNA is made from a nucleotides which are made from phosphate, a sugar, and one of four nitrogenous bases. The four nitrogenous bases are adenine, thymine, cytosine, and guanine. Based on this cytosine bonds with guanine, and thymine binds with guanine to form b ...
PowerPoint
PowerPoint

... is the process by which DNA fragments are drawn through an agarose gel from a negative to a positive charge due to the negative charge of the phosphate group on the single strand DNA.  The technique used to transfer DNA patterns for reading is called Southern ...
Chapter 9 DNA: The Genetic Material
Chapter 9 DNA: The Genetic Material

... Protein Synthesis / Gene Expression (steps involved in making a protein). 1. Transcription - instructions are transferred (rewritten) from DNA to a molecule of mRNA (messenger RNA). (occurs in the nucleus)  RNA polymerase binds to genes promoter (sequence that signals process to start.)  DNA stran ...
bio-of-cells-lent-restriction-enzymes-information-for-exam
bio-of-cells-lent-restriction-enzymes-information-for-exam

... This technique can be make more informative using southern blotting, where the fractionated DNA is transferred to a nylon membrane, and then specific sequences can be detected and their length determined by interrogating the membrane with a complementary DNA probe, which hybridises with the fragment ...
MUTATIONS TAKS QUESTIONS SPRING 2003 – 10: (22) The
MUTATIONS TAKS QUESTIONS SPRING 2003 – 10: (22) The

... 20 The diagram shows three generations of cells produced by a single cell through mitosis. In the process, a single mutation occurred at the point indicated. The mutation caused changes within a dominant allele. How many of the 15 cells contain the mutation? Record and bubble in your answer on the a ...
Viruses and Bacteria
Viruses and Bacteria

... to certain bases in DNA, is associated with reduced transcription in some species  In some species, DNA methylation causes longterm inactivation of genes in cellular differentiation  In genomic imprinting, methylation turns off either the maternal or paternal alleles of certain genes at the start ...
DNA VACCINES
DNA VACCINES

... Bacterial cell ...
Protein Synthesis Notes Review
Protein Synthesis Notes Review

... 2. To make proteins, what does the DNA have to be decoded into? 3. What are the three parts that make up a RNA nucleotide? 4. What are the three differences between DNA and RNA? 5. If a DNA chain had the following sequence, CCGTAATAGCAT, what RNA nucleotides would attach to this sequence? 6. What is ...
Recombinant and Synthetic Nucleic Acid Activity Registration
Recombinant and Synthetic Nucleic Acid Activity Registration

... Consist entirely of DNA segments from a single nonchromosomal or viral DNA source, though one or more of the segments may be a synthetic equivalent. Consist entirely of DNA from a prokaryotic host including its indigenous plasmids or viruses when propagated only in that host (or a closely related st ...
Entry Test Sample for MS in Bioinformatics Program Weightage Distribution:
Entry Test Sample for MS in Bioinformatics Program Weightage Distribution:

... C. Each of R and S is adjacent to Z. Which of the following is a pair of countries that can be the same color? A. R and S B. S and W C. W and X D. X and Y 3. Many surveys _____ out the idea that effective communication is essential for success and promotion in every field. A. are bearing ...
EXAM 2
EXAM 2

... genereation is referred to as _somatic______________, while those that can be passed on are referred to as _gametic___________. 37. An alteration in the DNA composition that is only expressed under certain circumstances (e.g. temperature) is referred to as __conditional mutation___________. 38. A ta ...
nature v. nurture
nature v. nurture

... predispositions for lots of diseases, like diabetes, cancer and heart disease." Human cells have tens of thousands of genes inside them, each with its own job, such as producing energy or overseeing cell division. But only certain genes are active at any given time or in any cell type while the rest ...
genome433
genome433

... break between them D. PHYSICAL: measures distances between genetic elements in terms of the LENGTH OF DNA between them. ...
12_Clicker_Questions
12_Clicker_Questions

... Restriction enzymes are useful tools for cutting DNA fragments. What do you think is the function of restriction enzymes in their normal bacterial environment? a. Restriction enzymes remove and recycle old mRNAs. b. Restriction enzymes cut up DNA taken from the environment and used as a nutrient sou ...
Comp 5c-2 Packet
Comp 5c-2 Packet

... Change in __________________ caused by change in structure of the DNA Gene mutations can be caused by DNA bases being: When genes are added or removed, the mutation is called a ________ ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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