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REVIEW 5: GENETICS 1. Chromosomes
REVIEW 5: GENETICS 1. Chromosomes

... DNA fingerprinting, or gel electrophoresis, creates banded patterns based on a person’s DNA base sequence. ...
Instructional Objectives—DNA, RNA and Protein Synthesis
Instructional Objectives—DNA, RNA and Protein Synthesis

... Objective 3: Explain how nucleotides are arranged in DNA and RNA.  If DNA is a ladder, where are sugars and phosphates located? Nitrogen bases? On the sides of the ladder. NB are on the rungs.  DNA is double stranded, but RNA is Single stranded Objective 4: Relate the structure of DNA to its funct ...
slides
slides

... •  Predict how different mutations in the DNA affect RNA and protein in different ways •  Explain how changes to chromosome structure and presence and absence of cell-specific transcription factors dictate which genes get transcribed and ultimately translated ...
Library types
Library types

... • Restriction mapping – The human genome project ...
Nucleotide is composed of a ribose sugar, a base and a phosphate
Nucleotide is composed of a ribose sugar, a base and a phosphate

... DNA strands: The start of the gene is always closest to the 5' end • Template strand/ non-coding strand/ antisense (3' to 5') strand – The template used for transcription • Non-template strand/ coding strand/ sense (5' to 3') strand – Codes for the gene. Same sequence as the new RNA strand (T replac ...
Reproduction and Genetics
Reproduction and Genetics

... Students relate changes in amino acid sequences to changes in the three-dimensional structure of the protein, which may alter its function. Students interpret charts and graphs, make scientific drawings, and label scientific diagrams. ...
Basic principles of DT40
Basic principles of DT40

... • Good model for genome stability in mammals • Complementation by human genes • Good database ...
Common Assessment Review
Common Assessment Review

... Nucleotide- subunit of nucleic acid. Composed of a nitrogenous base, 5-carbon sugar, and a phosphate group 2. What is the order of steps in protein synthesis (known as the central dogma of biology)? Define replication, transcription and translation. Replication transcription translation Replication- ...
Genes in Context Gene–Environment Interplay
Genes in Context Gene–Environment Interplay

... question: ‘‘If the effects of genetic variation can vary depending on characteristics of the environment, then what are environments doing to genes to alter their impact?’’ To address this question, we must first address the following question: ‘‘What do genes do?’’ Historically, gene was a term use ...
ppt - Department of Plant Sciences
ppt - Department of Plant Sciences

... 1. What are the established methods to determine if a plant is transgenic and whether the transgene(s) is expressed? 2. In a Southern or northern blot, through what type of chemical bond does the complementary probe bind to nucleic acid? 3. Nucleic acids and proteins are separated according to size ...
Ch. 13 Genetic Engineering
Ch. 13 Genetic Engineering

... The Enzymes cut the strands. The cut produces DNA fragments with short strands on each end that are complementary to each other ...
PCR reading answers
PCR reading answers

... from abnormal tissue and cDNA from healthy tissue is mixed with the single strands of DNA (genes) on the microarray. 25. In step three, how can someone tell if the gene is expressed ? Generally, cDNA from healthy tissue is tagged with green markers (stains). cDNA from abnormal tissue is tagged with ...
DNA - Snow Elementary School
DNA - Snow Elementary School

... 1. Why is transcription necessary? Transcription makes messenger RNA (MRNA) to carry the code for proteins out of the nucleus to the ribosomes in the cytoplasm. 2. Describe transcription. RNA polymerase binds to DNA, separates the strands, then uses one strand as a template to assemble MRNA. 3. Why ...
Sample Questions for EXAM III
Sample Questions for EXAM III

... 1. p53 is important for control of the cell cycle in very specific cell types that can spread when they become cancerous. 2. the p53 protein can move from cell to cell, causing cancer. 3. p53 is a kinase, which can phosphorylate many different cells cycle proteins. 4. p53 is involved in cell-cycle r ...
DNA Packing
DNA Packing

... – Recombinant DNA is formed by joining DNA sequences from two different sources: ...
Genetic Technology Discussion
Genetic Technology Discussion

... DNA is extracted from cells and mixed with restriction enzymes which cut the DNA at specific sequences Gel electrophoresis: DNA fragments are exposed to electrical current and separate leaving a unique pattern…a DNA “fingerprint”  DNA is negatively charged so it moves towards the + end  Short frag ...
No Slide Title
No Slide Title

... Transcription of b-interferon gene is activated during viral infection. ...
Powerpoint - Wishart Research Group
Powerpoint - Wishart Research Group

... Mix the City DNA with the Path DNA and let them randomly anneal (ligate with enzyme) After annealing/ligation they will form (7-2)! different long (150 bp) DNA molecules Select DNA molecules with the right start and ends (select by PCR) and length (gel) Sequence the DNA to determine the best pathway ...
genetics, dna replication, protein synthesis, biotechnology
genetics, dna replication, protein synthesis, biotechnology

... 2. For a given trait, the two genes of an allelic pair are not alike. An individual possessing this gene combination is said to be a. Homozygous for that trait b. Heterozygous for hat trait c. Recessive for that trait d. Pure for that trait 3. Curly hair in humans, white fur in guinea pigs, and need ...
Blotting : Southern, Northern and Western techniques
Blotting : Southern, Northern and Western techniques

... • DNA in the gel is placed on the filter paper with wigs dipped in a reservoir containing transfer buffer. • Nitrocellulose or Nylon membrane can be used for transfer. • Nitrocellulose has a binding capacity of 100µg/cm, while nylon has a binding capacity of about 500 µg/cm. • Membrane is placed on ...
Timeline of Genetic Engineering
Timeline of Genetic Engineering

... Gene Therapy 1. Process of changing a gene to treat a medical disease or disorder. 2. Absent or faulty gene is replaced by a normal, working gene. 3. This process allows the body to make the protein or enzyme it needs, which eliminates the cause of the disorder. ...
The Production of a
The Production of a

... Transformation – the uptake and expression of foreign DNA by a cell Transduction – the use of viruses to transform or genetically engineer cells Endonucleases – enzymes that cut RNA or DNA at specific sites; restriction enzymes are endonucleases that cut DNA Sticky cells – restriction fragments in w ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) ISSN: 2278-3008.
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) ISSN: 2278-3008.

... this reaction as a methyl group donor and also DNA methyltransferases (DNMT) play role in it [7]. DNA methylation, in alliance with histone adaptation is an indispensable constituent of the epigenetic mechanism, which controls gene expression and chromatin building. Throughout the human genome, CpG ...
ppt - Faculty
ppt - Faculty

... DNA replication involves a great many building blocks, enzymes and a great deal of ATP energy. DNA replication in humans occurs at a rate of 50 nucleotides per second and ~500/second in prokaryotes. Nucleotides have to be assembled and available in the nucleus, along with energy to make bonds betwe ...
How are we different? …at the RNA level.
How are we different? …at the RNA level.

... • In fact (in 2002), there was only one well understood biochemical difference, – A 92 bp deletion in a gene that codes for a hydroxylase, results in an un-hydroxylated secretion protein in our immune system. ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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