development, the Linker histone H1 is essential for Drosophila
... ;188 bp to ;176 bp. Furthermore, we observed depletion of the chromatosome band in the H1-depleted chromatin as compared with the control chromatin (Fig. 1D). Thus, bulk native chromatin contains substantially less H1 in pINT-1-H1 + Tubulin-GAL4 animals. To confirm that the lethality of pINT-1-H1 + ...
... ;188 bp to ;176 bp. Furthermore, we observed depletion of the chromatosome band in the H1-depleted chromatin as compared with the control chromatin (Fig. 1D). Thus, bulk native chromatin contains substantially less H1 in pINT-1-H1 + Tubulin-GAL4 animals. To confirm that the lethality of pINT-1-H1 + ...
Leukaemia Section t(5;12)(p13;p1 3) NIPBL/ETV6 Atlas of Genetics and Cytogenetics
... Cytogenetics molecular FISH showed that the breakpoint on 12p13 was located in ETV6. To confirm the position of the breakpoint on chromosome 5, BACs located at 5p13 were used as probes in FISH experiments. Analysis on BM cells of the patient with RP11-140A7 showed that one signal hybridized to the n ...
... Cytogenetics molecular FISH showed that the breakpoint on 12p13 was located in ETV6. To confirm the position of the breakpoint on chromosome 5, BACs located at 5p13 were used as probes in FISH experiments. Analysis on BM cells of the patient with RP11-140A7 showed that one signal hybridized to the n ...
supplementary information - Molecular Systems Biology
... enriched regulators of the co-regulated genes. Each subnetwork was constructed using the shortest molecular interaction paths connecting the seed gene to the positive or negative influence gene-set via enriched transcription factors (Table S3). Pathways involving five or greater links were discarded ...
... enriched regulators of the co-regulated genes. Each subnetwork was constructed using the shortest molecular interaction paths connecting the seed gene to the positive or negative influence gene-set via enriched transcription factors (Table S3). Pathways involving five or greater links were discarded ...
17 - Rutgers Chemistry
... The chloramphenicol acetyltransferase (CAT) gene is a popular reporter gene. This is a bacterial gene that evolved to protect bacteria against the antibiotic chloramphenicol (CAM). The gene encodes for a protein, chloramphenicol acetyltransferase (CAT), that can add an acetyl group (from acetyl CoA) ...
... The chloramphenicol acetyltransferase (CAT) gene is a popular reporter gene. This is a bacterial gene that evolved to protect bacteria against the antibiotic chloramphenicol (CAM). The gene encodes for a protein, chloramphenicol acetyltransferase (CAT), that can add an acetyl group (from acetyl CoA) ...
Reporter Genes and Traps
... These DNA elements are sometimes inserted into the endogenous gene so that the reporter will be expressed in a similar pattern as the endogenous gene. The disruption mutates the endogenous gene. Gene traps have splice acceptor sequences instead of having a promoter so that reporter gene‘s activity c ...
... These DNA elements are sometimes inserted into the endogenous gene so that the reporter will be expressed in a similar pattern as the endogenous gene. The disruption mutates the endogenous gene. Gene traps have splice acceptor sequences instead of having a promoter so that reporter gene‘s activity c ...
Comparison of the NSF45K Array Data with Other Microarray
... Identification of the Primarily Light-induced Genes among those Encoding Seven Components in the Rice Chlorophyll Biosynthesis Pathway To assess the usefulness of our microarray data we surveyed the expression patterns we obtained through microarray analysis for seventeen genes, including gene famil ...
... Identification of the Primarily Light-induced Genes among those Encoding Seven Components in the Rice Chlorophyll Biosynthesis Pathway To assess the usefulness of our microarray data we surveyed the expression patterns we obtained through microarray analysis for seventeen genes, including gene famil ...
A multistep epigenetic switch enables the stable
... that partial expression is not due to the fact that the deletions shift the a mutant in which these sequences were deleted (cHNL332; Fig. 4c). sequences of upstream genes closer to agn43 (cHNL330; Fig. 2d). The above experiments identified two different partial states. One Together, these data sugge ...
... that partial expression is not due to the fact that the deletions shift the a mutant in which these sequences were deleted (cHNL332; Fig. 4c). sequences of upstream genes closer to agn43 (cHNL330; Fig. 2d). The above experiments identified two different partial states. One Together, these data sugge ...
Frequent and histological type-specific inactivation of 14-3
... mers, suggesting `partial' methylation. In total, nine SCLC cell lines (69%) demonstrated hypermethylation of the 14-3-3s gene. The other four SCLC cell lines showed unmethylated patterns. In addition to the SCLC lines, large cell lung cancer lines among NSCLC cell lines also frequently showed hyper ...
... mers, suggesting `partial' methylation. In total, nine SCLC cell lines (69%) demonstrated hypermethylation of the 14-3-3s gene. The other four SCLC cell lines showed unmethylated patterns. In addition to the SCLC lines, large cell lung cancer lines among NSCLC cell lines also frequently showed hyper ...
Nature vs Nurture - YES! in Rock County
... – Creation of “new families” (eg. affiliation with gangs, ...
... – Creation of “new families” (eg. affiliation with gangs, ...
HL Protein Synthesis Question Sheet
... The process of protein synthesis has two main stages: transcription and translation. Transcription is the process of making an mRNA copy of the DNA. Translation is the use of mRNA and tRNA by ribosomes to synthesise a polypeptide chain. Although the processes are similar in both prokaryotes and euka ...
... The process of protein synthesis has two main stages: transcription and translation. Transcription is the process of making an mRNA copy of the DNA. Translation is the use of mRNA and tRNA by ribosomes to synthesise a polypeptide chain. Although the processes are similar in both prokaryotes and euka ...
Ch24- Memory Systems - Biology Courses Server
... The Temporal Lobes and Declarative Memory • Radial arm maze (a) – (b) Normal rats go down each arm for food only once, but with hippocampal lesions revisit arms already explored – (c) Normal and lesioned rats learn which arms are baited and avoid the rest, but still revisit arms (don’t remember tha ...
... The Temporal Lobes and Declarative Memory • Radial arm maze (a) – (b) Normal rats go down each arm for food only once, but with hippocampal lesions revisit arms already explored – (c) Normal and lesioned rats learn which arms are baited and avoid the rest, but still revisit arms (don’t remember tha ...
CHAPTER 19
... tail, so one could add a primer that consists of many T’s, called a poly-dT primer. After the complementary DNA strand has been made, the sample would then be mixed with primers, Taq polymerase, and nucleotides and subjected to the standard PCR protocol. Note: the PCR reaction would have two kinds ...
... tail, so one could add a primer that consists of many T’s, called a poly-dT primer. After the complementary DNA strand has been made, the sample would then be mixed with primers, Taq polymerase, and nucleotides and subjected to the standard PCR protocol. Note: the PCR reaction would have two kinds ...
Chapter Eleven: Chromosome Structure and Transposable Elements
... 3 × 107 nucleosomes × 9 histones = 2.7 × 108 molecules of histones are complexed to 6 billion bp of DNA. *24. Would you expect to see more or less acetylation in regions of DNA that are sensitive to digestion by DNase I? Why? More acetylation. Regions of DNase I sensitivity are less condensed than D ...
... 3 × 107 nucleosomes × 9 histones = 2.7 × 108 molecules of histones are complexed to 6 billion bp of DNA. *24. Would you expect to see more or less acetylation in regions of DNA that are sensitive to digestion by DNase I? Why? More acetylation. Regions of DNase I sensitivity are less condensed than D ...
Article Conserved Higher-Order Chromatin Regulates NMDA Receptor Gene Expression and Cognition Neuron
... expression. We generated stable HEK subclones with tet-on transactivator controlled induction of the histone methyltransferase SET-domain Bifurcated 1 (SETDB1/ESET/KMT1E), a previously described negative regulator of GRIN2B expression which confers, in human cell lines and mouse brain, repressive hi ...
... expression. We generated stable HEK subclones with tet-on transactivator controlled induction of the histone methyltransferase SET-domain Bifurcated 1 (SETDB1/ESET/KMT1E), a previously described negative regulator of GRIN2B expression which confers, in human cell lines and mouse brain, repressive hi ...
HbF inducers
... been shown to induce HbF synthesis in vitro ● Some studies have been carried out using valproic acid, a compound related to short fatty acid derivatives, reported increased HbF levels ...
... been shown to induce HbF synthesis in vitro ● Some studies have been carried out using valproic acid, a compound related to short fatty acid derivatives, reported increased HbF levels ...
Maternal plasma folate during pregnancy impacts differential DNA
... – ALSPAC, GenR, INMA, CHS, Project Viva ...
... – ALSPAC, GenR, INMA, CHS, Project Viva ...
Genome-wide identification of mononuclear cell DNA methylation
... donates methyl groups for various biological functions, including methylation of DNA. Methylation of DNA is one example of epigenetic regulation, and epigenetic regulation has been closely linked to development of human diseases6,7. New hypotheses have emerged that diet may affect metabolism via epi ...
... donates methyl groups for various biological functions, including methylation of DNA. Methylation of DNA is one example of epigenetic regulation, and epigenetic regulation has been closely linked to development of human diseases6,7. New hypotheses have emerged that diet may affect metabolism via epi ...
study guide for Vlad
... an experimental technique used to identify and select for individuals who possess a phenotype of interest (Wikipedia) A 180 base pair sequence in DNA that codes for a binding domain. Is important in controlling morphological patterns in animal, fungal, and plant development. (Wikipedia/Freeman text) ...
... an experimental technique used to identify and select for individuals who possess a phenotype of interest (Wikipedia) A 180 base pair sequence in DNA that codes for a binding domain. Is important in controlling morphological patterns in animal, fungal, and plant development. (Wikipedia/Freeman text) ...
Nonconventional Initiation Complex Assembly by STAT and NF
... A. Nos2 expression required signaling through both the IFN and NF-κB pathways -> ΔStat1 or ΔRela (NF-κB p65) genes suppressed iNOS mRNA induction B. IFN-I receptor (Ifnar1-/- mice) & the NF-κB pathway (Rela-/- & Ikbkb-/- mice, deficient for NF-κB p65 and the IKKβ kinase, respectively) & the ISGF3 ( ...
... A. Nos2 expression required signaling through both the IFN and NF-κB pathways -> ΔStat1 or ΔRela (NF-κB p65) genes suppressed iNOS mRNA induction B. IFN-I receptor (Ifnar1-/- mice) & the NF-κB pathway (Rela-/- & Ikbkb-/- mice, deficient for NF-κB p65 and the IKKβ kinase, respectively) & the ISGF3 ( ...
excercise handout
... Associated Brain Region(s)_________ Associated Disease(s) ____________________ Judgment (check one): Is potentially interesting Is not potentially interesting PMID ...
... Associated Brain Region(s)_________ Associated Disease(s) ____________________ Judgment (check one): Is potentially interesting Is not potentially interesting PMID ...
Learning to Remember Rare Events
... Figure 3: Extended Neural GPU with memory module. Memory query is read from the position one below the current output logit, and the embedded memory value is put at the same position of the output tape p. The network learns to use these values to produce the output in the next step. Sequence-to-sequ ...
... Figure 3: Extended Neural GPU with memory module. Memory query is read from the position one below the current output logit, and the embedded memory value is put at the same position of the output tape p. The network learns to use these values to produce the output in the next step. Sequence-to-sequ ...
Supplementary Information (doc 28K)
... siRNA (Cy). After 24 hours cells were incubated in the presence or absence of hypoxic condition (1%O2, 5%CO2 atmosphere or CoCl2 treatment) for 12 hours. HIF-1 mRNA expression was evaluated by RT-PCR whereas HIF-1 protein level and activity was detected by western blot and ELISA, respectively, as ...
... siRNA (Cy). After 24 hours cells were incubated in the presence or absence of hypoxic condition (1%O2, 5%CO2 atmosphere or CoCl2 treatment) for 12 hours. HIF-1 mRNA expression was evaluated by RT-PCR whereas HIF-1 protein level and activity was detected by western blot and ELISA, respectively, as ...
De Novo Nonsense Mutations in KAT6A, a Lysine Acetyl
... processes including the cell-cycle, cell differentiation, metabolism, and apoptosis. Nonsense mutations in genes that are involved in histone acetylation and deacetylation result in multiple congenital anomalies with most individuals displaying significant developmental delay, microcephaly and dysmo ...
... processes including the cell-cycle, cell differentiation, metabolism, and apoptosis. Nonsense mutations in genes that are involved in histone acetylation and deacetylation result in multiple congenital anomalies with most individuals displaying significant developmental delay, microcephaly and dysmo ...