Supplementary Information (doc 28K)
... siRNA (Cy). After 24 hours cells were incubated in the presence or absence of hypoxic condition (1%O2, 5%CO2 atmosphere or CoCl2 treatment) for 12 hours. HIF-1 mRNA expression was evaluated by RT-PCR whereas HIF-1 protein level and activity was detected by western blot and ELISA, respectively, as ...
... siRNA (Cy). After 24 hours cells were incubated in the presence or absence of hypoxic condition (1%O2, 5%CO2 atmosphere or CoCl2 treatment) for 12 hours. HIF-1 mRNA expression was evaluated by RT-PCR whereas HIF-1 protein level and activity was detected by western blot and ELISA, respectively, as ...
Genetics and Epigenetics of Human Disease
... single gene disorders (see Figure 4). Diseases where one defective gene or section of DNA is involved are named ‘Mendelian’ disorders after him. Knowing that there is just one place to look for the mutation causing such a condition has helped scientists to discover which gene is linked to which Mend ...
... single gene disorders (see Figure 4). Diseases where one defective gene or section of DNA is involved are named ‘Mendelian’ disorders after him. Knowing that there is just one place to look for the mutation causing such a condition has helped scientists to discover which gene is linked to which Mend ...
Introduction Exercise 1: Measuring gene expression
... Can you find on the gene cards page an association with MCF-7 cells? Hint: what was MCF-7 for type of cell line again? Each line in the file represents one gene; the 2nd column represents the gene symbol. The Pvalue is a probability that represents the chance to find the expression values that belon ...
... Can you find on the gene cards page an association with MCF-7 cells? Hint: what was MCF-7 for type of cell line again? Each line in the file represents one gene; the 2nd column represents the gene symbol. The Pvalue is a probability that represents the chance to find the expression values that belon ...
Behavioral Objectives
... might carry animal viruses into humans. (HIV is a virus that jumped from monkeys into humans.) Have students read the Health Focus “Organs for Transplant”. Discuss some of the fears brought out in the article. General Public’s Understanding of Biotechnology 2. Students should read the Bioethical Foc ...
... might carry animal viruses into humans. (HIV is a virus that jumped from monkeys into humans.) Have students read the Health Focus “Organs for Transplant”. Discuss some of the fears brought out in the article. General Public’s Understanding of Biotechnology 2. Students should read the Bioethical Foc ...
ashavsky 2006
... declarative memory is unlikely to be based on such a non-stable foundation as synaptic plasticity. Memory that can last throughout an entire lifespan should be ‘‘etched in stone.’’ The only ‘‘stone-like’’ molecules within living cells are DNA molecules. Therefore, I advocate an alternative, genomic ...
... declarative memory is unlikely to be based on such a non-stable foundation as synaptic plasticity. Memory that can last throughout an entire lifespan should be ‘‘etched in stone.’’ The only ‘‘stone-like’’ molecules within living cells are DNA molecules. Therefore, I advocate an alternative, genomic ...
slides
... Since 1990 programs are no longer limited to searching for independent exons, but try instead to identify the whole complex structure of a gene. Given a sequence and using signal sensors, one can accumulate evidence on the occurrence of signals: translation starts and stops and splice sites are th ...
... Since 1990 programs are no longer limited to searching for independent exons, but try instead to identify the whole complex structure of a gene. Given a sequence and using signal sensors, one can accumulate evidence on the occurrence of signals: translation starts and stops and splice sites are th ...
ppt
... • Constructed a large, real-life, heterogeneous benchmark for testing motif finding tools • Demonstrated various applications of motif discovery ...
... • Constructed a large, real-life, heterogeneous benchmark for testing motif finding tools • Demonstrated various applications of motif discovery ...
GcvA, a LysR-type transcriptional regulator protein
... Isolation of cefotaxime (Ctx)-sensitive mutants. Mutagenesis of JRG582(pNU305) was performed using N-methyl-"-nitroN-nitrosoguanidine (NTG) according to the method of Maniatis e t al. (1982). Ctx-sensitive mutants were identified by replica-plating colonies on to nutrient medium containing 50 pg Ctx ...
... Isolation of cefotaxime (Ctx)-sensitive mutants. Mutagenesis of JRG582(pNU305) was performed using N-methyl-"-nitroN-nitrosoguanidine (NTG) according to the method of Maniatis e t al. (1982). Ctx-sensitive mutants were identified by replica-plating colonies on to nutrient medium containing 50 pg Ctx ...
Meta-analysis of Prefrontal Cortex from Acute Ethanol Studies
... important in the analysis of microarray data because it can give clues to underlying mechanisms of regulation of a set of genes. Possible treatment for drugs of abuse can be formulated by targeting specific genes in a gene network. The mechanisms underlying the development of alcoholism remain large ...
... important in the analysis of microarray data because it can give clues to underlying mechanisms of regulation of a set of genes. Possible treatment for drugs of abuse can be formulated by targeting specific genes in a gene network. The mechanisms underlying the development of alcoholism remain large ...
manual - GSA-SNP
... should represent high associations. Some data types have their own parameters: Data type ...
... should represent high associations. Some data types have their own parameters: Data type ...
Expression of an aphid-induced barley methyltransferase in
... methyltransferase, OMT It is also induced by the jasmonic acid signalling pathway OMTs generally – methylate caffeic acid – lead to lignin precursors – or various classes of flavonoids ...
... methyltransferase, OMT It is also induced by the jasmonic acid signalling pathway OMTs generally – methylate caffeic acid – lead to lignin precursors – or various classes of flavonoids ...
Overview of the Recombinant DNA technology- the plasmid vector pUC19
... to manipulate DNA in order to return it to living organisms where it can function normally. Essentially, it involves isolating a piece of DNA from an organism and introducing it into a cloning host, for example bacterium Escherichia Coli which grows and divides rapidly. It is therefore possible to s ...
... to manipulate DNA in order to return it to living organisms where it can function normally. Essentially, it involves isolating a piece of DNA from an organism and introducing it into a cloning host, for example bacterium Escherichia Coli which grows and divides rapidly. It is therefore possible to s ...
William’s syndrome: gene expression is related to ORIGINAL ARTICLE
... controls on the x axis and that for WS subjects on the y axis. The relative levels of expression are generally similar in WS and normal subjects (Figure 1b). However, there is a set of genes whose correlations with GTF2I differ notably between WS and control subjects, including several where the cor ...
... controls on the x axis and that for WS subjects on the y axis. The relative levels of expression are generally similar in WS and normal subjects (Figure 1b). However, there is a set of genes whose correlations with GTF2I differ notably between WS and control subjects, including several where the cor ...
Biology 4.7 Gene Types
... activated and when and where. Could be able to explain the purpose of variations in gene activity. ...
... activated and when and where. Could be able to explain the purpose of variations in gene activity. ...
University of Groningen Regulation of hepatobiliary transport
... one of the major functions of the liver. Membrane transport proteins that primarily determine bile formation are located at the basolateral (uptake) and canalicular (secretion) membrane domains of hepatocytes. The last step in hepatobiliary transport, i.e., the secretion of the major bile constituen ...
... one of the major functions of the liver. Membrane transport proteins that primarily determine bile formation are located at the basolateral (uptake) and canalicular (secretion) membrane domains of hepatocytes. The last step in hepatobiliary transport, i.e., the secretion of the major bile constituen ...
Microarray Analysis of Drosophila Development During
... (before the late larval ecdysone pulse) but then fall to low or undetectable levels during this pulse. This category is colored red in the first column. They are potentially repressed by ecdysone The second category consists of genes expressed at low or undetectable levels before the late larval ecd ...
... (before the late larval ecdysone pulse) but then fall to low or undetectable levels during this pulse. This category is colored red in the first column. They are potentially repressed by ecdysone The second category consists of genes expressed at low or undetectable levels before the late larval ecd ...
Document
... heat shock proteins and chaperons) are highly expressed at 48˚C than are at lower temperatures, indicating that the fungus is under heat stress. 2. More putative virulence genes (ex. those coding for the proteins responsive to oxidative stress and host immune system and for toxin production) are hig ...
... heat shock proteins and chaperons) are highly expressed at 48˚C than are at lower temperatures, indicating that the fungus is under heat stress. 2. More putative virulence genes (ex. those coding for the proteins responsive to oxidative stress and host immune system and for toxin production) are hig ...
Chapter Sixteen: Control of Gene Expression
... as hypersensitivity to DNase I. The reverse reaction by deacetylases stabilizes nucleosome structure and lessens DNase I sensitivity. Other transcriptional factors and regulatory proteins, called chromatin remodeling complexes, bind directly to the DNA, altering chromatin structure without acetylati ...
... as hypersensitivity to DNase I. The reverse reaction by deacetylases stabilizes nucleosome structure and lessens DNase I sensitivity. Other transcriptional factors and regulatory proteins, called chromatin remodeling complexes, bind directly to the DNA, altering chromatin structure without acetylati ...
Molecular genetics in Streptococcus thermophilus
... Mollet, unpublished results). Similarly, mutations are being analysed which cause a decrease in the expession of the 2 genes. Using the ~-galactosidase minus ST11 host strain, work is ongoing on the characterization of other important signal sequences involved in gene expression. Promoter probe vect ...
... Mollet, unpublished results). Similarly, mutations are being analysed which cause a decrease in the expession of the 2 genes. Using the ~-galactosidase minus ST11 host strain, work is ongoing on the characterization of other important signal sequences involved in gene expression. Promoter probe vect ...
Insulin-like signaling pathway: flies and mammals
... metabolism, while IGF-1R promotes growth. • IGF-1R is activated by its ligand IGF-1, which is secreted in response to growth hormone. • Pathway more complicated: more tissue specific signaling and regulation. – Multiple homologs, some specific to certain somatic tissues. – Genetic investigation is m ...
... metabolism, while IGF-1R promotes growth. • IGF-1R is activated by its ligand IGF-1, which is secreted in response to growth hormone. • Pathway more complicated: more tissue specific signaling and regulation. – Multiple homologs, some specific to certain somatic tissues. – Genetic investigation is m ...
CRS questions
... 3) It has been estimated that approximately half of human genes yield mRNAs of different sequences because the splicing of introns can vary among different tissues and cellular circumstances. Consider what would be the consequence of splicing RNAs in different ways; different proteins would be produ ...
... 3) It has been estimated that approximately half of human genes yield mRNAs of different sequences because the splicing of introns can vary among different tissues and cellular circumstances. Consider what would be the consequence of splicing RNAs in different ways; different proteins would be produ ...
ppt for
... Recent studies have reported rare and de novo mutations in ASD, but the allelic architecture of ASD remains unclear. To assess the role of common and rare variations in ASD, we constructed a gene co-expression network based on a widespread survey of gene expression in the human brain. We identified ...
... Recent studies have reported rare and de novo mutations in ASD, but the allelic architecture of ASD remains unclear. To assess the role of common and rare variations in ASD, we constructed a gene co-expression network based on a widespread survey of gene expression in the human brain. We identified ...
Leukaemia Section t(5;12)(q31;p13) in MDS, AML and AEL in Oncology and Haematology
... (AML) with eosinophilia, and a patient with acute eosinophilic leukemia (AEL). ...
... (AML) with eosinophilia, and a patient with acute eosinophilic leukemia (AEL). ...