High-Resolution Single-Copy Gene Fluorescence in Situ
... Koumbaris and Bass (2003) have developed a strategy to avoid the cross-hybridization of repetitive sequences using sorghum (Sorghum bicolor) BACs as probes to the maize chromosome 9 in an oat (Avena sativa)–maize addition line. The most direct and effective way to construct cytogenetic maps for orga ...
... Koumbaris and Bass (2003) have developed a strategy to avoid the cross-hybridization of repetitive sequences using sorghum (Sorghum bicolor) BACs as probes to the maize chromosome 9 in an oat (Avena sativa)–maize addition line. The most direct and effective way to construct cytogenetic maps for orga ...
Full-Text PDF
... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
Genetics of Skin Colour
... externally visible characteristics, including skin, hair and eye colour from DNA has important forensic implications. This information can be used, backed-up by legislation, to provide identifiable characteristics of perpetrators from DNA recovered from a crime scene. Such technology will also permi ...
... externally visible characteristics, including skin, hair and eye colour from DNA has important forensic implications. This information can be used, backed-up by legislation, to provide identifiable characteristics of perpetrators from DNA recovered from a crime scene. Such technology will also permi ...
Word file: Analysis of alternative splicing in Drosophila
... sample is then washed repeatedly in PBT. To block non-specific antibody binding the tissue is then placed in 500 µl PBT supplemented with 5% Normal Goat Serum (Sigma) at room temperature and incubated for 1hour. The tissue is then immersed in PBT + 5% NGS along with primary antibodies directed at bo ...
... sample is then washed repeatedly in PBT. To block non-specific antibody binding the tissue is then placed in 500 µl PBT supplemented with 5% Normal Goat Serum (Sigma) at room temperature and incubated for 1hour. The tissue is then immersed in PBT + 5% NGS along with primary antibodies directed at bo ...
chapter 1 introduction: themes in the study of life
... • Contains DNA that is segregated from the rest of the cell. DNA is organized with proteins into chromosomes that are located within the nucleus, the largest organelle of most cells. • Cytoplasm surrounds the nucleus and contains various organelles of different functions • Some cells have a tough ce ...
... • Contains DNA that is segregated from the rest of the cell. DNA is organized with proteins into chromosomes that are located within the nucleus, the largest organelle of most cells. • Cytoplasm surrounds the nucleus and contains various organelles of different functions • Some cells have a tough ce ...
Microarray-based comparative genomic hybridisation (array CGH)
... (where a section of a chromosome is inverted or reversed), will not be identified using array CGH. This is because balanced chromosome rearrangements do not result in any loss or gain of chromosome material. It will also not detect some types of polyploidy (more than the usual 2 sets of chromosomes) ...
... (where a section of a chromosome is inverted or reversed), will not be identified using array CGH. This is because balanced chromosome rearrangements do not result in any loss or gain of chromosome material. It will also not detect some types of polyploidy (more than the usual 2 sets of chromosomes) ...
- Philsci-Archive
... primary interest is in the content of the notions themselves and less in the empirical details of the illustrations. For example, it is commonly claimed that the causal relationship between DNA sequence and the proteins for which it “codes” is “specific”. My concern is with what this claim means—wit ...
... primary interest is in the content of the notions themselves and less in the empirical details of the illustrations. For example, it is commonly claimed that the causal relationship between DNA sequence and the proteins for which it “codes” is “specific”. My concern is with what this claim means—wit ...
Lecture 10: Reproduction II: Sexual Reproduction and Meiosis
... 90% of the time required for meiosis • Chromosomes begin to condense • In synapsis, homologous chromosomes loosely pair up, aligned gene by gene • This is very different from mitosis, where the homologous chromosomes ignore one ...
... 90% of the time required for meiosis • Chromosomes begin to condense • In synapsis, homologous chromosomes loosely pair up, aligned gene by gene • This is very different from mitosis, where the homologous chromosomes ignore one ...
13LecturePresentation
... Comparison of Asexual and Sexual Reproduction • Asexual reproduction - one parent produces genetically identical offspring by mitosis • Clone -group of genetically identical individuals from the same parent • Sexual reproduction - two parents give rise to offspring that have unique combinations of ...
... Comparison of Asexual and Sexual Reproduction • Asexual reproduction - one parent produces genetically identical offspring by mitosis • Clone -group of genetically identical individuals from the same parent • Sexual reproduction - two parents give rise to offspring that have unique combinations of ...
Caenorhabditis elegans chromosome arms are anchored to the
... LEM-2 at the inner nuclear membrane, while the central regions are not. The large LEM-2 domains at the arms consist of smaller subdomains, which are characterized by a high density of repetitive sequences and a low density of genes. These subdomains are transcriptionally inactive, whereas the gaps b ...
... LEM-2 at the inner nuclear membrane, while the central regions are not. The large LEM-2 domains at the arms consist of smaller subdomains, which are characterized by a high density of repetitive sequences and a low density of genes. These subdomains are transcriptionally inactive, whereas the gaps b ...
Word - The Open University
... At this stage, we should distinguish testing from genetic screening. Genetic testing is used with individuals who, because of their family history think they are at risk of carrying the gene for a particular genetic disease. Screening covers wide-scale testing of populations, to discover who may be ...
... At this stage, we should distinguish testing from genetic screening. Genetic testing is used with individuals who, because of their family history think they are at risk of carrying the gene for a particular genetic disease. Screening covers wide-scale testing of populations, to discover who may be ...
Parental Age Affects Somatic Mutation Rates in
... given in “Materials and Methods“ under the heading ”Statistical Analysis.” No asterisk indicates no significant difference. ...
... given in “Materials and Methods“ under the heading ”Statistical Analysis.” No asterisk indicates no significant difference. ...
1 Meiotic sex chromosome inactivation is disrupted in
... testis cDNA. Efficiencies of 95-105% were obtained for all primers. For experimental assays, 16µl reactions with a final concentration of 300nM of each primer were run in 96well plates for 40 cycles with annealing at 60°C. We analyzed 3-5 (FACS cells) or three (testes) biological replicates per geno ...
... testis cDNA. Efficiencies of 95-105% were obtained for all primers. For experimental assays, 16µl reactions with a final concentration of 300nM of each primer were run in 96well plates for 40 cycles with annealing at 60°C. We analyzed 3-5 (FACS cells) or three (testes) biological replicates per geno ...
Role of the p21 Protein in the Fanconi Anemia Pathway
... – Biallelic mutations in any one of thirteen genes results in FA – Autosomal and X-linked ...
... – Biallelic mutations in any one of thirteen genes results in FA – Autosomal and X-linked ...
No Slide Title
... • Anomalies may be detected molecularly providing new tools for cancer screening or detection of relapse • Since the cell-cycle is essential for cell-proliferation, inhibitors of the cell-cycle are anti-proliferative agents useful in a variety of clinical settings (cancer, inflammation, re-stenosis ...
... • Anomalies may be detected molecularly providing new tools for cancer screening or detection of relapse • Since the cell-cycle is essential for cell-proliferation, inhibitors of the cell-cycle are anti-proliferative agents useful in a variety of clinical settings (cancer, inflammation, re-stenosis ...
Recombineering Hunchback identifies two conserved domains
... al., 2001; Walsh and Reid, 1995). However, as progenitors change over time to accommodate the production of different tissues, they also undergo a progressive restriction and lose their competence to produce the full assortment of cell types (Desai and McConnell, 2000; Rapaport et al., 2001). The ab ...
... al., 2001; Walsh and Reid, 1995). However, as progenitors change over time to accommodate the production of different tissues, they also undergo a progressive restriction and lose their competence to produce the full assortment of cell types (Desai and McConnell, 2000; Rapaport et al., 2001). The ab ...
Gibberellin Signaling: Biosynthesis, Catabolism, and
... biosynthesis during seed germination may be part of the mechanism that modulates the synthesis of bioactive GAs by regulating the transport of intermediate(s) between cells. Alternatively, this phenomenon may be simply the evolutionary consequence of this pathway. When detailed knowledge of the loca ...
... biosynthesis during seed germination may be part of the mechanism that modulates the synthesis of bioactive GAs by regulating the transport of intermediate(s) between cells. Alternatively, this phenomenon may be simply the evolutionary consequence of this pathway. When detailed knowledge of the loca ...
François Jacob
... expression levels in cells is a result of feedback on the transcription of DNA sequences. Their experiments and ideas gave impetus to the emerging field of molecular developmental biology, and of transcriptional regulation in particular. For many years it had been known that bacterial and other cell ...
... expression levels in cells is a result of feedback on the transcription of DNA sequences. Their experiments and ideas gave impetus to the emerging field of molecular developmental biology, and of transcriptional regulation in particular. For many years it had been known that bacterial and other cell ...
The Ubiquitin-Proteasome Pathway and Plant Development
... many HECT E3s have been identified in animals (up to 50 putative HECT E3s in humans, for example), only seven HECT E3s are encoded by the Arabidopsis genome (Schwarz et al., 1998; Downes et al., 2003). The first examples of plant HECT E3s, UPL1 (for Ubiquitin Protein Ligase1)and UPL2, were reported ...
... many HECT E3s have been identified in animals (up to 50 putative HECT E3s in humans, for example), only seven HECT E3s are encoded by the Arabidopsis genome (Schwarz et al., 1998; Downes et al., 2003). The first examples of plant HECT E3s, UPL1 (for Ubiquitin Protein Ligase1)and UPL2, were reported ...
LECTURE 13: EPIGENETICS – IMPRINTING Reading: Ch. 18, p
... paternal imprinting, half the progeny of affected females will be affected and (2) in maternal imprinting, half the progeny of affected males will be affected. Why does imprinting exist? Imprinting seems to put individuals at a disadvantage, giving them only one chance for an active copy of the gene ...
... paternal imprinting, half the progeny of affected females will be affected and (2) in maternal imprinting, half the progeny of affected males will be affected. Why does imprinting exist? Imprinting seems to put individuals at a disadvantage, giving them only one chance for an active copy of the gene ...
SILK-BASED DELIVERY OF BIOACTIVE MOLECULES
... Model of receptor mediated transfection via silk-based cationic block copolymers with ligands or functional peptides. (a) Formation of ion complexes between gene(s) and silk-polylysine block copolymers. (b) Binding of the complex to the cell via specific receptors or membrane proteins such as integr ...
... Model of receptor mediated transfection via silk-based cationic block copolymers with ligands or functional peptides. (a) Formation of ion complexes between gene(s) and silk-polylysine block copolymers. (b) Binding of the complex to the cell via specific receptors or membrane proteins such as integr ...
RAPD mapping of three QTLs determining trichome - UvA-DARE
... the location and developmental timing of type D trichomcs seems to be genetically regulated (Bachmann et al. 1987; Bachmann and Chambers 1990). Where they appear first on leaves of vegetative rosettes, they are restricted to the angles of the leaf teeth or to the abaxial (under) side of the leaf tip ...
... the location and developmental timing of type D trichomcs seems to be genetically regulated (Bachmann et al. 1987; Bachmann and Chambers 1990). Where they appear first on leaves of vegetative rosettes, they are restricted to the angles of the leaf teeth or to the abaxial (under) side of the leaf tip ...
Role of aquaporins in leaf physiology
... To understand the role of individual AQP in leaves, information on tissue-specific expression is essential. Translational fusion, in situ hybridization, and immunolocalization are the most common approaches presently used to study the tissue-specific expression of a single isoform. Table 1 presents ...
... To understand the role of individual AQP in leaves, information on tissue-specific expression is essential. Translational fusion, in situ hybridization, and immunolocalization are the most common approaches presently used to study the tissue-specific expression of a single isoform. Table 1 presents ...
Acute myeloid leukemia - Apoptotic signalling and gene expression
... by a clonal expansion of immature myeloid cells in the bone marrow, resulting in severe infections and bleedings. High dose chemotherapy is able to normalize the blood and bone marrow morphology (complete remission, CR) in a majority of treated patients, but recurrent disease, typically occurs withi ...
... by a clonal expansion of immature myeloid cells in the bone marrow, resulting in severe infections and bleedings. High dose chemotherapy is able to normalize the blood and bone marrow morphology (complete remission, CR) in a majority of treated patients, but recurrent disease, typically occurs withi ...