Plant Molecular Biology
... All plant seeds were sterilized by subsequent soakings in concentrated sulfuric acid and bleach as described by Van Brussel et al. [23]. After germination, seedlings with a tooth length of about 1 cm were transferred to test tubes containing Jensen agar slants [23]. After incubation of the seedlings ...
... All plant seeds were sterilized by subsequent soakings in concentrated sulfuric acid and bleach as described by Van Brussel et al. [23]. After germination, seedlings with a tooth length of about 1 cm were transferred to test tubes containing Jensen agar slants [23]. After incubation of the seedlings ...
light control of seedling development
... Perhaps the most important component for encoding the complexity of responses is the multiple families of photoreceptors. Seedling responses to light are mediated by at least three classes of regulatory photoreceptors: (a) phytochromes, which respond mainly to red and far-red light but which also ab ...
... Perhaps the most important component for encoding the complexity of responses is the multiple families of photoreceptors. Seedling responses to light are mediated by at least three classes of regulatory photoreceptors: (a) phytochromes, which respond mainly to red and far-red light but which also ab ...
Unbalanced translocation, a major chromosome alteration
... Unbalanced translocation could also be an additional responsible alteration due to the following reasons. This alteration was shown to cause LOH of the VHL tumor suppressor gene locus in individuals of a family predisposed to renal cell cancer who carried a constitutional reciprocal chromosome trans ...
... Unbalanced translocation could also be an additional responsible alteration due to the following reasons. This alteration was shown to cause LOH of the VHL tumor suppressor gene locus in individuals of a family predisposed to renal cell cancer who carried a constitutional reciprocal chromosome trans ...
Tum/RacGAP50C provides a critical link between anaphase
... centrosomes, grow to contact the cell cortex, and an actomyosin-based contractile ring assembles where the cell will ultimately cleave. In recent years many proteins involved in cytokinesis have been identified (Giansanti et al., 2004; Glotzer, 2005; Skop et al., 2004), and we are beginning to under ...
... centrosomes, grow to contact the cell cortex, and an actomyosin-based contractile ring assembles where the cell will ultimately cleave. In recent years many proteins involved in cytokinesis have been identified (Giansanti et al., 2004; Glotzer, 2005; Skop et al., 2004), and we are beginning to under ...
Cilia are at the heart of vertebrate left–right asymmetry
... across larger regions of the embryo. Finally, regional molecular asymmetry must be converted into asymmetric organogenesis. Over the past decade, much progress has been made elucidating asymmetrically expressed molecules that comprise a pathway that signals small-scale LR asymmetry across the embryo ...
... across larger regions of the embryo. Finally, regional molecular asymmetry must be converted into asymmetric organogenesis. Over the past decade, much progress has been made elucidating asymmetrically expressed molecules that comprise a pathway that signals small-scale LR asymmetry across the embryo ...
Introduction - Stellenbosch University
... 8. thickEnd - The ending position at which the feature is drawn thickly (for example the stop ...
... 8. thickEnd - The ending position at which the feature is drawn thickly (for example the stop ...
The KIebsieIIa pneumoniae cytochrome bd
... The molecular and genetic characterization of the E. coli cytochrome bd’ has been extensive. Subunit I (cytochrome b5,&is encoded by cydA, and subunit I1 (which contains haem b,,, and haem d ) is encoded by cydB (Green et al., 1988). The cydAB genes are co-transcribed from a promoter upstream of cyd ...
... The molecular and genetic characterization of the E. coli cytochrome bd’ has been extensive. Subunit I (cytochrome b5,&is encoded by cydA, and subunit I1 (which contains haem b,,, and haem d ) is encoded by cydB (Green et al., 1988). The cydAB genes are co-transcribed from a promoter upstream of cyd ...
HMW glutenin subunits in multiploid Aegilops species: composition
... 1981 that the composition of HMW glutenin subunits in a given common wheat variety could affect the baking quality of its flour[4]. Recent biotechnological investigations on transgenic expression of the genes encoding the functionally superior HMW subunit (such as the 1Dx5 subunit specified by the G ...
... 1981 that the composition of HMW glutenin subunits in a given common wheat variety could affect the baking quality of its flour[4]. Recent biotechnological investigations on transgenic expression of the genes encoding the functionally superior HMW subunit (such as the 1Dx5 subunit specified by the G ...
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... probe that binds Nitrosococcus sequences accounted for only 2.0 to 2.6% of the relative fluorescence signal, suggesting that the abundance of this gammaproteobacterial AOB were sufficiently low and uniform that their omission from the qPCR amplification does not change the observed trends. By contra ...
... probe that binds Nitrosococcus sequences accounted for only 2.0 to 2.6% of the relative fluorescence signal, suggesting that the abundance of this gammaproteobacterial AOB were sufficiently low and uniform that their omission from the qPCR amplification does not change the observed trends. By contra ...
Development of Co-Dominant Amplified Polymorphic Sequence
... most cost-effective means to mitigate disease losses. However, monogenic resistance commonly is rendered ineffective if frequency of corresponding virulence alleles increases in the pathogen population (15). Strategies that would create more durably resistant cultivars presently are focusing upon th ...
... most cost-effective means to mitigate disease losses. However, monogenic resistance commonly is rendered ineffective if frequency of corresponding virulence alleles increases in the pathogen population (15). Strategies that would create more durably resistant cultivars presently are focusing upon th ...
The mitochondrial genome of the soybean cyst nematode
... touch-down PCR, in which the cycling conditions remain the same, but the annealing temperature is increased to 65 °C (for 20 s), dropping by 1 °C each cycle. The touch-down approach is designed to amplify only those double-stranded molecules produced during cycle 6 (reducing the amount of nonspecifi ...
... touch-down PCR, in which the cycling conditions remain the same, but the annealing temperature is increased to 65 °C (for 20 s), dropping by 1 °C each cycle. The touch-down approach is designed to amplify only those double-stranded molecules produced during cycle 6 (reducing the amount of nonspecifi ...
Respiratory terminal oxidases in the facultative chemoheterotrophic
... site of a respiratory electron transport chain. The two processes share several components, making cyanobacteria the only cells in which these two most important bioenergetic processes occur in the same cellular compartment. The cytoplasmic membrane, in contrast, does not contain a functional photos ...
... site of a respiratory electron transport chain. The two processes share several components, making cyanobacteria the only cells in which these two most important bioenergetic processes occur in the same cellular compartment. The cytoplasmic membrane, in contrast, does not contain a functional photos ...
1 Supplemental Table 1. FACS-isolated, SSEA-4
... growth, and follicle survival 35, -mutations in this gene are associated with cancer. -may play a role in the development and normal function of the ovaries, defects in this gene have been linked to premature ovarian failure 2, -could be involved in oogenesis. -this gene encodes an orphan nuclear ...
... growth, and follicle survival 35, -mutations in this gene are associated with cancer. -may play a role in the development and normal function of the ovaries, defects in this gene have been linked to premature ovarian failure 2, -could be involved in oogenesis. -this gene encodes an orphan nuclear ...
phylogenetic analysis of the rompb genes of rickettsia felis and
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
Recombinant DNA technology - The Centre for Plant Sciences
... soluble, and then to concentrate the DNA by precipitation with alcohols and salt, followed by resuspension in a small volume of an aqueous buffer at slightly alcaline pH (7.5-8). One can then visualise the DNA on a gel. The detection limit of this method is approximately 10 nanograms (nano gram mea ...
... soluble, and then to concentrate the DNA by precipitation with alcohols and salt, followed by resuspension in a small volume of an aqueous buffer at slightly alcaline pH (7.5-8). One can then visualise the DNA on a gel. The detection limit of this method is approximately 10 nanograms (nano gram mea ...
A preanaphase role for a Cks/Suc1 in acentrosomal spindle
... mammalian genomes also have two Cks genes, they are more similar in sequence to each other than to either of the two cks genes in Drosophila (Fig 4A). We next examined the gene expression pattern of the two cks genes during Drosophila development. RNAs were isolated from various stages of developmen ...
... mammalian genomes also have two Cks genes, they are more similar in sequence to each other than to either of the two cks genes in Drosophila (Fig 4A). We next examined the gene expression pattern of the two cks genes during Drosophila development. RNAs were isolated from various stages of developmen ...
The Biochemical Machinery of Plastid Envelope
... the information present in their own genome but are strongly dependent on imported proteins that are encoded in the nuclear genome and translated in the cytoplasm. Plastids are present in every plant cell, with very few exceptions (such as the highly specialized male sexual cells), and their structu ...
... the information present in their own genome but are strongly dependent on imported proteins that are encoded in the nuclear genome and translated in the cytoplasm. Plastids are present in every plant cell, with very few exceptions (such as the highly specialized male sexual cells), and their structu ...
Three-dimensional Structures of Bulge
... usually resulting in a non-functioning gene product. Frameshifts that result from unpaired nucleotides can arise from recombination processes or from displacement of bases during replication (Streisinger et al., 1966), e.g. when the template strand contains an unpaired base, the progeny strand will ...
... usually resulting in a non-functioning gene product. Frameshifts that result from unpaired nucleotides can arise from recombination processes or from displacement of bases during replication (Streisinger et al., 1966), e.g. when the template strand contains an unpaired base, the progeny strand will ...
Non-invasive prenatal assessment of trisomy 21 by multiplexed
... at high risk of fetal trisomy 21. The traditional method of identifying the high risk group has been increased maternal age, but screening by this method would require invasive testing in about 5% of pregnant women and identify only 30% of affected fetuses.1 In the past 20 years maternal age has bee ...
... at high risk of fetal trisomy 21. The traditional method of identifying the high risk group has been increased maternal age, but screening by this method would require invasive testing in about 5% of pregnant women and identify only 30% of affected fetuses.1 In the past 20 years maternal age has bee ...
Localization and structural analysis of the ribosomal RNA operons of
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
Familial Adenomatous Polyposis (FAP)
... Some people develop benign growths in their abdomen called desmoid tumours. These tumours sometimes need to be removed if they cause symptoms but they do not become cancerous. There is no need to have regular checks for desmoids but if you have any lumps or unexplained symptoms, you should discuss t ...
... Some people develop benign growths in their abdomen called desmoid tumours. These tumours sometimes need to be removed if they cause symptoms but they do not become cancerous. There is no need to have regular checks for desmoids but if you have any lumps or unexplained symptoms, you should discuss t ...
Identification of Functional Connections Between Calmodulin and the Yeast Actin Cytoskeleton.
... Three independent libraries containing CMD1 random substitution mutations (i.e., altered sequences still capable of producing functional calmodulin) at positions 89–92 were used in this study. Plasmids containing these CMD1 mutations were recovered from yeast, amplified in E. coli, and transformed b ...
... Three independent libraries containing CMD1 random substitution mutations (i.e., altered sequences still capable of producing functional calmodulin) at positions 89–92 were used in this study. Plasmids containing these CMD1 mutations were recovered from yeast, amplified in E. coli, and transformed b ...
A pair of Sox: distinct and overlapping functions of
... and sox9b transcripts accumulate in overlapping and unique domains. Gene expression, however, does not equate to gene function. The jef mutation destroys sox9a activity (Yan et al., ...
... and sox9b transcripts accumulate in overlapping and unique domains. Gene expression, however, does not equate to gene function. The jef mutation destroys sox9a activity (Yan et al., ...
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... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ...
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ...