Towards Programmable Molecular Machines
... (also known as branch migration) or by enzymes that can break the backbone of DNA at locations specified by the DNA sequence itself (restriction enzymes) and link two DNA backbones together (ligation enzymes). Many of these machines are self-assembled and several of them move along a 1- or 2- dimens ...
... (also known as branch migration) or by enzymes that can break the backbone of DNA at locations specified by the DNA sequence itself (restriction enzymes) and link two DNA backbones together (ligation enzymes). Many of these machines are self-assembled and several of them move along a 1- or 2- dimens ...
Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... its ATPase activity using a sensitive fluorescence assay which measures the production of Pi [28]. As the ATPase activity of the isolated ATPase domain is quite low, 15 μM protein was used. Figure 1 shows an ATP titration for the ATPase domain. An ATP-dependent increase in activity was observed, and ...
... its ATPase activity using a sensitive fluorescence assay which measures the production of Pi [28]. As the ATPase activity of the isolated ATPase domain is quite low, 15 μM protein was used. Figure 1 shows an ATP titration for the ATPase domain. An ATP-dependent increase in activity was observed, and ...
the pathogenesis, pathology and immunology of smallpox and
... women were delivered of live infants just before admission to hospital, labour having begun at the time of the pre-eruptive fever. Marsden & Greenfield (1934) reported an analysis of 34 of the cases concerned. In 17 cases the baby escaped in utero infection. In 2 of these babies, born during the mot ...
... women were delivered of live infants just before admission to hospital, labour having begun at the time of the pre-eruptive fever. Marsden & Greenfield (1934) reported an analysis of 34 of the cases concerned. In 17 cases the baby escaped in utero infection. In 2 of these babies, born during the mot ...
Hox Genes: Let`s Work Together
... means posteriorly expressed Hox genes can still block the activity of anteriorly expressed Hox gene even in the case of forced expression of anterior Hox gene using different promoter and only translated regions of the gene (Gonzalez-Reyes and Morata, 1990). This convincingly suggests that posterior ...
... means posteriorly expressed Hox genes can still block the activity of anteriorly expressed Hox gene even in the case of forced expression of anterior Hox gene using different promoter and only translated regions of the gene (Gonzalez-Reyes and Morata, 1990). This convincingly suggests that posterior ...
Journal of Bacteriology
... uberolysin (28), butyrivibriocin (29), gassericin (30), and lactocyclicin (31). For this reason, it was assumed that the product of the gene cluster around RBAM_029230 (in the following, named acn) might be involved in the synthesis and processing of a circular peptide which is the putative antibact ...
... uberolysin (28), butyrivibriocin (29), gassericin (30), and lactocyclicin (31). For this reason, it was assumed that the product of the gene cluster around RBAM_029230 (in the following, named acn) might be involved in the synthesis and processing of a circular peptide which is the putative antibact ...
Bacterial viruses targeting multi- resistant Klebsiella
... Phage infection The main concern of phages is temporal: to ensure that their genes are transcribed in the correct order so that the host cell is not lysed before progeny virions are assembled. This process is commonly called a genetic cascade, where each set of genes that gets transcribed during th ...
... Phage infection The main concern of phages is temporal: to ensure that their genes are transcribed in the correct order so that the host cell is not lysed before progeny virions are assembled. This process is commonly called a genetic cascade, where each set of genes that gets transcribed during th ...
using polymerase chain reaction for detection of enterotoxigenic
... encodes the A subunit of the toxin, and ctxB gene encodes the B subunit. PCR technique and specific ctxA primer were used to detect enterotoxigenic. Vibrio cholerae cells were treated by SDS 10%, or by boiling to release DNA. Using the PCR technique could not detect directly Vibrio cholerae in seafo ...
... encodes the A subunit of the toxin, and ctxB gene encodes the B subunit. PCR technique and specific ctxA primer were used to detect enterotoxigenic. Vibrio cholerae cells were treated by SDS 10%, or by boiling to release DNA. Using the PCR technique could not detect directly Vibrio cholerae in seafo ...
Optimizing cofactor availability for the production of recombinant
... Co-overexpressions of HEM genes with HRP. HRP overexpression was regulated by PAOX1, co-overexpressions of eGFP and HEM were regulated by PGAP. Bars are average values of HRP production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations f ...
... Co-overexpressions of HEM genes with HRP. HRP overexpression was regulated by PAOX1, co-overexpressions of eGFP and HEM were regulated by PGAP. Bars are average values of HRP production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations f ...
Colicins produced by the Escherichia fergusonii strains closely
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
Characterization of chaperonin 10 (Cpn10)
... Identification, isolation and characterization of the E. histolytica gene encoding Cpn10 Searches of preliminary data generated by the E. histolytica genome project at the Sanger Institute revealed several clones with sequence similarity to the human Cpn10 protein sequence. PCR primers based on thes ...
... Identification, isolation and characterization of the E. histolytica gene encoding Cpn10 Searches of preliminary data generated by the E. histolytica genome project at the Sanger Institute revealed several clones with sequence similarity to the human Cpn10 protein sequence. PCR primers based on thes ...
Phosphatidylglycerol-containing ER
... contacts between organelles could accommodate transfer of calcium, the process of mitochondrial membranes repair and restitution, and the demand to coordinate nuclear gene expression and maintain regulatory processes in response to cellular need for ATP synthesis were not plausible [3,4,16-18]. The ...
... contacts between organelles could accommodate transfer of calcium, the process of mitochondrial membranes repair and restitution, and the demand to coordinate nuclear gene expression and maintain regulatory processes in response to cellular need for ATP synthesis were not plausible [3,4,16-18]. The ...
WJEC s13-biology1
... conferences were held shortly after the papers were taken so that reference could be made to the full range of candidates' responses, with photocopied scripts forming the basis of discussion. The aim of the conferences was to ensure that the marking schemes were interpreted and applied in the same w ...
... conferences were held shortly after the papers were taken so that reference could be made to the full range of candidates' responses, with photocopied scripts forming the basis of discussion. The aim of the conferences was to ensure that the marking schemes were interpreted and applied in the same w ...
Inheritance of White Colour in Alpacas
... at the time of the Spanish conquest, and to subsequent lack of research into the field. In very recent times, molecular genetics tools have been made available to the alpaca research community that allow us to study pigmentation in alpacas much more easily and thoroughly. These tools include the alp ...
... at the time of the Spanish conquest, and to subsequent lack of research into the field. In very recent times, molecular genetics tools have been made available to the alpaca research community that allow us to study pigmentation in alpacas much more easily and thoroughly. These tools include the alp ...
Analyzing the Paper
... After picking MC1R as their candidate gene, the Mogil group set out to determine whether its gene product is actually required for activity of the female-specific analgesia pathway. Formal proof that they picked the correct gene would require the group to engineer their own knock-out mouse and then ...
... After picking MC1R as their candidate gene, the Mogil group set out to determine whether its gene product is actually required for activity of the female-specific analgesia pathway. Formal proof that they picked the correct gene would require the group to engineer their own knock-out mouse and then ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
Yeast lipid metabolism at a glance
... Tamura et al., 2012). Therefore, a lipid transport system must exist to translocate lipids from their site of synthesis to their final destination (Achleitner et al., 1999; Schnabl et al., 2005; Peretti et al., 2008). Due to the very well-conserved mechanisms between all eukaryotes and due to other ...
... Tamura et al., 2012). Therefore, a lipid transport system must exist to translocate lipids from their site of synthesis to their final destination (Achleitner et al., 1999; Schnabl et al., 2005; Peretti et al., 2008). Due to the very well-conserved mechanisms between all eukaryotes and due to other ...
Regulation of meiotic progression by the meiosis
... transmitted through a transduction pathway usually formed by protein kinases. Ultimately, checkpoint effectors act on cellular targets, triggering various responses, including cell cycle arrest or delay and DNA repair. In mammals, defects in checkpoint responses cause genomic instability, leading to ...
... transmitted through a transduction pathway usually formed by protein kinases. Ultimately, checkpoint effectors act on cellular targets, triggering various responses, including cell cycle arrest or delay and DNA repair. In mammals, defects in checkpoint responses cause genomic instability, leading to ...
B1 topic 1 questions
... C mutualism D parasitism (ii) Some bees have evolved a new method of collecting nectar from flowers. They drill a small hole in the base of the flower and collect the nectar through the hole. This means the pollen does not stick to the bee. Suggest why this is an advantage to the bees. ...
... C mutualism D parasitism (ii) Some bees have evolved a new method of collecting nectar from flowers. They drill a small hole in the base of the flower and collect the nectar through the hole. This means the pollen does not stick to the bee. Suggest why this is an advantage to the bees. ...
Alu - Environmental
... Alu elements • Alu elements are only found in the primate branch • Each Alu insertion is a unique event and is inherited from each parent • Most occurred millions of years ago and are often on both pairs of chromosomes • There are Alu elements that have occurred since humans branched from other pri ...
... Alu elements • Alu elements are only found in the primate branch • Each Alu insertion is a unique event and is inherited from each parent • Most occurred millions of years ago and are often on both pairs of chromosomes • There are Alu elements that have occurred since humans branched from other pri ...
Synthesis of Oligonucleotides
... chemistries and new nucleoside building blocks have been introduced, which require milder deprotection conditions at the end of the synthesis. For example, a matched set of phenoxyacetyl (PAC) for dA, isopropylphenoxyacetyl for dG and acetyl for dC can be removed by treatment with 0.05 M potassium c ...
... chemistries and new nucleoside building blocks have been introduced, which require milder deprotection conditions at the end of the synthesis. For example, a matched set of phenoxyacetyl (PAC) for dA, isopropylphenoxyacetyl for dG and acetyl for dC can be removed by treatment with 0.05 M potassium c ...
Epidemiological and molecular biological studies of
... facultative anaerobes. They are approximately 1 μm in size and grow in pigmented yellow or white colonies. They may occur singly or grouped in pairs, short chains or grape-like clusters. Staphylococci are catalase-positive, oxidase-negative and have different proteins, teikon acids and polysaccharid ...
... facultative anaerobes. They are approximately 1 μm in size and grow in pigmented yellow or white colonies. They may occur singly or grouped in pairs, short chains or grape-like clusters. Staphylococci are catalase-positive, oxidase-negative and have different proteins, teikon acids and polysaccharid ...
Feline Genetics: a Combinatorial Approach
... sharply instead of gradually: their effect shows up completely or not at all, according to which variant (allele) of the gene is considered. So, rather than DNA proteins, we shall have the combinatorics of symbols, standing for genes. Please be warned that the mathematical models are aimed to explai ...
... sharply instead of gradually: their effect shows up completely or not at all, according to which variant (allele) of the gene is considered. So, rather than DNA proteins, we shall have the combinatorics of symbols, standing for genes. Please be warned that the mathematical models are aimed to explai ...
Adrenomedullin Gene Expression Is Developmentally Regulated and
... homology to the calcitonin gene-related peptide (CGRP) family (4). It is capable of acting through the CGRP receptor and the recently cloned Adm receptor (AdmR) (5). The Adm peptide has been implicated as an important regulator in the renal and cardiovascular systems, where it has been observed to p ...
... homology to the calcitonin gene-related peptide (CGRP) family (4). It is capable of acting through the CGRP receptor and the recently cloned Adm receptor (AdmR) (5). The Adm peptide has been implicated as an important regulator in the renal and cardiovascular systems, where it has been observed to p ...
Cytokinin and auxin have a crucial role in the control of this balance.
... We also focus on recent progress in understanding the molecular mechanisms through which these hormones interact to control plant organ development. Cytokinin biosynthesis, transport and signalling Cytokinins came to light because of their ability to promote cell division in tobacco tissue culture [ ...
... We also focus on recent progress in understanding the molecular mechanisms through which these hormones interact to control plant organ development. Cytokinin biosynthesis, transport and signalling Cytokinins came to light because of their ability to promote cell division in tobacco tissue culture [ ...