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Genes have fixed positions on chromosomes.
Genes have fixed positions on chromosomes.

Cell fusion
Cell fusion

... So double knockout could be 0.00072~ 5X10-7. One 10cm tissue culture dish holds ~ 5x106 cells. Note: Same considerations for creation of recessive tumor suppressor genes in cancer: requires a double knockout. But there are lots of cells in a human tissue or in a mouse. RNAi screen, should knock down ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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