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Gene Expression
Gene Expression

... Cells use information in genes to build hundreds of different proteins, each with a specific function. But, not all proteins are required by the cell at any one time. By regulating gene expression, cells are able to control which proteins are made. ...
Key Area 2 – Pupil Booklet
Key Area 2 – Pupil Booklet

... 1. state that genes are located on chromosomes in the nucleus of every cell. 2. state that a gene is a section of DNA which controls an inherited characteristic. 3. state that genes are passed on from parents to offspring in the egg and ...
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... and -Complementation 1. Cut out gene of interest with restriction enzyme 2. Cut B/W cloning vector with same restriction enzyme (MCS) a. Dephosphorylate vector to prevent self-ligation 3. Mix insert with vector and add ligase 4. Transform E. coli that is made for B/W screening 5. Plate onto media t ...
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Science EQT Study Guide: 2nd Quarter

... Offspring that are hybrid (heterozygous) for a trait will have only the dominant trait in the phenotype. This is an example of which of Mendel's laws? In a plant, tall (D) is the dominant allele. Short (d) is the recessive allele. Which genotype would a short offspring have? In DNA, what can be said ...
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The History of Molecular Genetics

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DNA RNA PSyn notes

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... 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC ...
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... represent “genes”. Genes are regions on chromosomes that code for specific proteins. While many parts of that code are important parts of the “recipe”, some parts are simply “filler”, and are unnecessary, so far as we understand. These unnecessary sections are called introns, and they are sequences ...
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Alternative conceptions about genetics

... Single genes code for particular traits The examples we use when teaching about genetics may lead to the view that particular traits are always coded for by a single gene. While there are a few traits that are determined by a single gene (for example, dimples and cleft chin), most traits are complex ...
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... When λ enters a susceptible cell, the single-stranded tails anneal to generate a circle. The site formed by the single stranded tails is called COS (cohesive site) and the left and right arms become contiguous and form a single operon, the right hand operon. The central region comprises a second ope ...
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Looking Beyond Our DNA - Federation of American Societies for

... Two FDA-approved therapeutics, azacitidine and decitabine, which decrease DNA methylation, have been used to treat blood disorders called myelodysplastic syndromes by “reactivating” the tumor suppressor genes. Another class of FDA-approved epigenetic drugs, called histone deacetylase inhibitors, has ...
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Lecture#31 – Evolution and cis

... a. no affect on gene expression/phenotype -> no selection for/against b. random drift causes fixation of DNA sequence c. useful for markers in genetic mapping /DNA finger printing Result: Evolution occurs via random mutation and fixation by random drift – no selection 2) Gene’s coding sequences a. c ...
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DNA Review Questions

... 42. The function of tRNA is to A. provide a place for polypeptide synthesis B. transport amino acids to the ribosome C. travel to the ribosome to direct the assembly of polypeptides D. transcribes DNA E. translates DNA 43. The function of mRNA is to A. provide a place for polypeptide synthesis B. tr ...
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Spatial organization is a key difference between unicellular

... changes. Cells may change behaviour on a temporal plan, including but not limited to the cell cycle. Metazoan: Specialized cell functions and differentiation occur based on cell lineage and spatial location within a body plan. Within this body plan, cells retain their specialized function despite en ...
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... (A nucleus having) Two sets of chromosomes (or two copies of each chromosome) ...
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Agrobacterium tumefaciens

... a. It is normally not stably integrated into the plant cell b. It may be intolerant of changes to the organization of its genome c. Genome may show instability ...
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PCR Lab Notes

... There are 23 pairs of chromosomes which contains 30,000 to 50,000 genes. These genes only comprise about 5 % of chromosomal DNA. The other 95% is non-coding DNA. The sequence with the genes are introns, which is transcribed into RNA but in the end do not make a protein. ...
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doc Review of Lecture 27

... o Chemicals that resemble nucleotides o Can be integrated into DNA in place of nucleotides o Less stable forms than bases, shift base-pairing affinities & lead to changes o 5-bromouracil (5-BU) – causes A-T  G-C or G-C  A-T ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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