OBJECTIVE SHEET MICROBIOLOGY 1 PROKARYOTES 1. List the
... At first sight, it may seem that the conquest of disease is the most important reason for studying bacteria. It is well known that some bacteria can cause disease — although it should be borne in mind that many diseases are caused not by bacteria, but by viruses, fungi or protozoa. Diseases, which a ...
... At first sight, it may seem that the conquest of disease is the most important reason for studying bacteria. It is well known that some bacteria can cause disease — although it should be borne in mind that many diseases are caused not by bacteria, but by viruses, fungi or protozoa. Diseases, which a ...
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... trilobite families retain largely homonomous trunk segments (Eldridge, 1977). The Crustacea present a very different picture, with a Palaeozoic radiation giving rise to a huge diversity of body plans (Cisne, 1974; Schram, 1986; Briggs et ...
... trilobite families retain largely homonomous trunk segments (Eldridge, 1977). The Crustacea present a very different picture, with a Palaeozoic radiation giving rise to a huge diversity of body plans (Cisne, 1974; Schram, 1986; Briggs et ...
En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES
... are widespread among plants [8]. They share a common sequence (5’-CACTA-3’) at their TIR termini and were found in a variety of distantly related species [9-13], but only a few elements were characterized at the molecular level [7, 8]. For Arabidopsis, where the system was shown to transpose with hi ...
... are widespread among plants [8]. They share a common sequence (5’-CACTA-3’) at their TIR termini and were found in a variety of distantly related species [9-13], but only a few elements were characterized at the molecular level [7, 8]. For Arabidopsis, where the system was shown to transpose with hi ...
Bayesian Partition Models for Identifying Expression Quantitative
... of Pr(Y|X). Motivated by the success of BEAM, Zhang et al. (2010) developed a Bayesian partition (BP) model for eQTL studies based on a joint model of gene expression and SNPs. More specifically, correlated expression traits Y and their associated set of markers X are treated as a module in the BP m ...
... of Pr(Y|X). Motivated by the success of BEAM, Zhang et al. (2010) developed a Bayesian partition (BP) model for eQTL studies based on a joint model of gene expression and SNPs. More specifically, correlated expression traits Y and their associated set of markers X are treated as a module in the BP m ...
Networks in leaf development
... the leaf primordium and repress FIL expression on the adaxial side of the leaf primordium. This demonstrates that FIL is actively excluded from the adaxial domain [32]. The role of YABBY genes in abaxial specification is not clear, however, because loss-of-function mutants do not have obvious dorsov ...
... the leaf primordium and repress FIL expression on the adaxial side of the leaf primordium. This demonstrates that FIL is actively excluded from the adaxial domain [32]. The role of YABBY genes in abaxial specification is not clear, however, because loss-of-function mutants do not have obvious dorsov ...
Biochemistry of Starch and Cellulose Mark Guilenan The
... • Amylose: contains glucose residues connected via α‐1,4 linkages • Amylopec8n: contains a backbone of glucose residues linked through α‐1,4 linkages plus nonrandomly placed α‐1,6 branch‐points (5% of glycosidic bonds). The clustered nature of the α‐1,6 branch‐points allows glucan side chains ...
... • Amylose: contains glucose residues connected via α‐1,4 linkages • Amylopec8n: contains a backbone of glucose residues linked through α‐1,4 linkages plus nonrandomly placed α‐1,6 branch‐points (5% of glycosidic bonds). The clustered nature of the α‐1,6 branch‐points allows glucan side chains ...
Mutator Transposon in Maize and MULEs in the Plant Genome
... Mu insertions, especially germinally transmitted insertions [14]. Only two maize lines with active MuDR elements have been identified so far, but all maize lines carry MuDR elements derivatives, or homologous MuDR sequences (hMuDRs), whose coding sequences are 80%–99% identical to those of MuDR. Sur ...
... Mu insertions, especially germinally transmitted insertions [14]. Only two maize lines with active MuDR elements have been identified so far, but all maize lines carry MuDR elements derivatives, or homologous MuDR sequences (hMuDRs), whose coding sequences are 80%–99% identical to those of MuDR. Sur ...
- Wiley Online Library
... genes have been identified, which are expressed specifically at distinct phases of vascular tissue differentiation or in a subset of vascular tissue cell types (Hertzberg et al., 2001; Kuriyama and Fukuda, 2001; Zhao et al., 2000). The subsequent functional analysis of such genes and mutants display ...
... genes have been identified, which are expressed specifically at distinct phases of vascular tissue differentiation or in a subset of vascular tissue cell types (Hertzberg et al., 2001; Kuriyama and Fukuda, 2001; Zhao et al., 2000). The subsequent functional analysis of such genes and mutants display ...
The Tel-PDGFRß fusion gene produces a chronic
... founders and normal littermates were analyzed by flow cytometry. Peripheral blood was obtained from anesthetized mice by orbital bleeding. Mice were sacrificed by cervical dislocation, tissues were removed for histology and cell suspensions were made by gently rubbing one half spleen over a fine sta ...
... founders and normal littermates were analyzed by flow cytometry. Peripheral blood was obtained from anesthetized mice by orbital bleeding. Mice were sacrificed by cervical dislocation, tissues were removed for histology and cell suspensions were made by gently rubbing one half spleen over a fine sta ...
Human mitochondrial leucyl tRNA synthetase can suppress non
... IV. There was no change in CII activity, whilst induction of aaRS significantly increased CI and CIV activity, where in most cases there was at least a doubling of activity (Fig 2B). The analyses thus far were directed at recovery of function rather than any direct consequence on the mutant tRNAval. ...
... IV. There was no change in CII activity, whilst induction of aaRS significantly increased CI and CIV activity, where in most cases there was at least a doubling of activity (Fig 2B). The analyses thus far were directed at recovery of function rather than any direct consequence on the mutant tRNAval. ...
Joint analysis of the influence of CYP11B1 and DGAT1 genetic
... PCR Buffer, 1.5 mM MgCl2, 150 M each of dNTP, 0.5 pM each of forward and reverse primer, and 5% DMSO because of a GC-rich (66%) PCR product. The PCR profile included 1 cycle of 15 min at 95°C, followed by 10 cycles of 30 s at 94°C, 30 s at 72°C to 62°C (1°C less every cycle) and 45 s at 72°C, follo ...
... PCR Buffer, 1.5 mM MgCl2, 150 M each of dNTP, 0.5 pM each of forward and reverse primer, and 5% DMSO because of a GC-rich (66%) PCR product. The PCR profile included 1 cycle of 15 min at 95°C, followed by 10 cycles of 30 s at 94°C, 30 s at 72°C to 62°C (1°C less every cycle) and 45 s at 72°C, follo ...
Neuronal death is enhanced and begins during atrophy spinal cord
... ®rst and second trimesters of gestation was obtained from pregnancies terminated by dilatation and evacuation for social reasons. Gestational age was determined by ultrasound measurements. In total, 58 control samples were collected and analysed. Sixteen SMA foetuses were obtained from abortions aft ...
... ®rst and second trimesters of gestation was obtained from pregnancies terminated by dilatation and evacuation for social reasons. Gestational age was determined by ultrasound measurements. In total, 58 control samples were collected and analysed. Sixteen SMA foetuses were obtained from abortions aft ...
SQA CfE Higher Human Biology Unit 1: Human Cells
... ball of cells and then a hollow ball with a fluid-filled interior. Early in this process, the cells are unspecialised and are capable of developing into any of the body's cell types. Later, the cells lose this general ability and become increasingly limited in terms of their potential functions. How ...
... ball of cells and then a hollow ball with a fluid-filled interior. Early in this process, the cells are unspecialised and are capable of developing into any of the body's cell types. Later, the cells lose this general ability and become increasingly limited in terms of their potential functions. How ...
Assaying … promoter activity
... true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from this culture were plated onto selective (LB with 5 mg/L gentamicin) or non-select ...
... true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from this culture were plated onto selective (LB with 5 mg/L gentamicin) or non-select ...
Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
gene_expression
... Biological process: A recognized series of events or molecular functions (e.g., cell cycle, development, metabolism) Molecular function: What does a gene’s product do? (e.g., binding, enzyme activity, receptor activity) Cellular component: Localization within the cell (e.g., membrane, nucleus, ubiqu ...
... Biological process: A recognized series of events or molecular functions (e.g., cell cycle, development, metabolism) Molecular function: What does a gene’s product do? (e.g., binding, enzyme activity, receptor activity) Cellular component: Localization within the cell (e.g., membrane, nucleus, ubiqu ...
EOCT PREP - Reed Biology
... • 46) The finches that Darwin studied in the Galapagos islands have evolved with different types of beaks: Drilling, cracking, tearing and fishing. This is an example of: • A)protective resemblence • B)adaptive radiation • C)warning coloration • D)predation ...
... • 46) The finches that Darwin studied in the Galapagos islands have evolved with different types of beaks: Drilling, cracking, tearing and fishing. This is an example of: • A)protective resemblence • B)adaptive radiation • C)warning coloration • D)predation ...
Control of the acetamidase gene of Mycobacterium smegmatis by
... Much interest has been focussed on this system for its potential use in mycobacterial genetic studies. The availability of an inducible promoter which functions well in mycobacteria including the important human pathogen Mycobacterium tuberculosis would be extremely useful. The acetamidase system ha ...
... Much interest has been focussed on this system for its potential use in mycobacterial genetic studies. The availability of an inducible promoter which functions well in mycobacteria including the important human pathogen Mycobacterium tuberculosis would be extremely useful. The acetamidase system ha ...
Analysis of genetic mosaics in developing and adult Drosophila
... requires that the FRT sequence be located closer to the centromere than the gene of interest. We have introduced dominantly marked FRT sequences into the genome near the centromere on each major chromosome arm. We have constructed chromosomes that carry cell markers or adult cuticular markers in add ...
... requires that the FRT sequence be located closer to the centromere than the gene of interest. We have introduced dominantly marked FRT sequences into the genome near the centromere on each major chromosome arm. We have constructed chromosomes that carry cell markers or adult cuticular markers in add ...
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... breakpoints for these events is analyzed, short regions of sequence similarity are found in some cases. For instance, recombination between two similar genes that are several million bp apart can lead to deletion of the intervening genes in somatic cells. Site-specific recombination occurs between p ...
... breakpoints for these events is analyzed, short regions of sequence similarity are found in some cases. For instance, recombination between two similar genes that are several million bp apart can lead to deletion of the intervening genes in somatic cells. Site-specific recombination occurs between p ...
Genetic diversity and epidemiology of infectious hematopoietic
... 6 or 7 d post-inoculation. Infected cells and media for each virus isolate were centrifuged and 1.0 m1 aliquots of supernatant were frozen at -7O0C. These served as the virus stock used in the study. The reference virus strain, RB1, isolated in 1975 from steelhead Oncorhynchus mykiss at the Round Bu ...
... 6 or 7 d post-inoculation. Infected cells and media for each virus isolate were centrifuged and 1.0 m1 aliquots of supernatant were frozen at -7O0C. These served as the virus stock used in the study. The reference virus strain, RB1, isolated in 1975 from steelhead Oncorhynchus mykiss at the Round Bu ...
Molecular cloning of a cDNA encoding a novel Ca2+
... Ca2 , Mg2 , and Zn2 on the activity of the nuclease. The presence of EGTA (grid C2) and EDTA (grid C3) inhibited nuclease activity, as well as DNase I activity (grids A2 and A3). The addition of Ca2 led to an increase in the size of the pink halos (grid C4), indicating an enhancement of nuclease ...
... Ca2 , Mg2 , and Zn2 on the activity of the nuclease. The presence of EGTA (grid C2) and EDTA (grid C3) inhibited nuclease activity, as well as DNase I activity (grids A2 and A3). The addition of Ca2 led to an increase in the size of the pink halos (grid C4), indicating an enhancement of nuclease ...
AP & Regents Biology
... 1. The mechanism of action of restriction enzymes 2. The different results you would expect if a mutation occurred at the recognition site for enzyme Y. ...
... 1. The mechanism of action of restriction enzymes 2. The different results you would expect if a mutation occurred at the recognition site for enzyme Y. ...
DNA sequencing revealed a definitive
... Figure 4. Similarity matrix for the amino acid sequences of the homeo domains of iab-7 and its deuterostome cognates. Numbers represent % aa positional identity, which is 100 x the number of matched aa/60 aa. 72% aa positional identity, respectively (26,28,30,31,32). Also, the HB4 homeo domain share ...
... Figure 4. Similarity matrix for the amino acid sequences of the homeo domains of iab-7 and its deuterostome cognates. Numbers represent % aa positional identity, which is 100 x the number of matched aa/60 aa. 72% aa positional identity, respectively (26,28,30,31,32). Also, the HB4 homeo domain share ...