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... (which cleaves DNA 27 bp 3⬘ to its recognition site) digestion for 4 hours to generate a 27 bp sequence tag ligated to the biotin-labeled adaptor. The biotin-labeled tags were purified using 30 ml of strepavidin-Dynabeads (Invitrogen) per sample. After end repairing using Klenow polymerase, a second ...
... (which cleaves DNA 27 bp 3⬘ to its recognition site) digestion for 4 hours to generate a 27 bp sequence tag ligated to the biotin-labeled adaptor. The biotin-labeled tags were purified using 30 ml of strepavidin-Dynabeads (Invitrogen) per sample. After end repairing using Klenow polymerase, a second ...
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L
... taxus [41], sesame [42], and peanut [43]. However, transcriptomic information is lacking for Sacha Inchi. We were interested in identifying genes involved in PUFA biosynthesis (especially α-linolenic acid) in Sacha Inchi seeds during the period of fast oil accumulation; therefore, transcriptome anal ...
... taxus [41], sesame [42], and peanut [43]. However, transcriptomic information is lacking for Sacha Inchi. We were interested in identifying genes involved in PUFA biosynthesis (especially α-linolenic acid) in Sacha Inchi seeds during the period of fast oil accumulation; therefore, transcriptome anal ...
Dynamics and control of DNA sequence amplification
... operating conditions is required. However, in the absence of such a dynamic model, the operating conditions for PCR reactions are typically selected based on analysis of reaction thermodynamics, and, to a lesser extent, qualitative analysis of reaction kinetics. Reductions in cycle efficiency (eithe ...
... operating conditions is required. However, in the absence of such a dynamic model, the operating conditions for PCR reactions are typically selected based on analysis of reaction thermodynamics, and, to a lesser extent, qualitative analysis of reaction kinetics. Reductions in cycle efficiency (eithe ...
MHF1 plays Fanconi anaemia complementation group M protein
... exhibit meiotic defects similar to those of fancm mutants in A. thaliana. Therefore, we analysed the meiotic stages of DAPI-stained chromosome spreads from mhf1-1 and mhf1-2 pollen mother cells and WT cells that were undergoing meiosis using fluorescence microscopy. In the pachytene stage, the DAPI- ...
... exhibit meiotic defects similar to those of fancm mutants in A. thaliana. Therefore, we analysed the meiotic stages of DAPI-stained chromosome spreads from mhf1-1 and mhf1-2 pollen mother cells and WT cells that were undergoing meiosis using fluorescence microscopy. In the pachytene stage, the DAPI- ...
Identification of proteins that putatively bind the
... investigating the genes involved in stomatal development has been done in the eudicot model system, Arabidopsis thaliana. However, orthologs have been identified in the monocot species rice (Oryza sativa) and corn (Zea mays) suggesting that the genes involved in this process are conserved. Arabidops ...
... investigating the genes involved in stomatal development has been done in the eudicot model system, Arabidopsis thaliana. However, orthologs have been identified in the monocot species rice (Oryza sativa) and corn (Zea mays) suggesting that the genes involved in this process are conserved. Arabidops ...
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility
... phosphorylation and activation of the transcription factors, for example, IFN regulatory factors 3 and 7, or activation of the nuclear factor of kappa B (NF-kB) transcription factor system.12–14 Both signalling cascades can trigger the expression of type I IFN ...
... phosphorylation and activation of the transcription factors, for example, IFN regulatory factors 3 and 7, or activation of the nuclear factor of kappa B (NF-kB) transcription factor system.12–14 Both signalling cascades can trigger the expression of type I IFN ...
The rapidly evolving field of plant centromeres
... satellite evolution is driven by the selection and coevolution of satellites and centromere-binding proteins, rather than by random genetic drift. This requires that certain centromere satellite variants confer a selective advantage upon meiotic cells (i.e. eggs and sperm), a process known as meioti ...
... satellite evolution is driven by the selection and coevolution of satellites and centromere-binding proteins, rather than by random genetic drift. This requires that certain centromere satellite variants confer a selective advantage upon meiotic cells (i.e. eggs and sperm), a process known as meioti ...
Genetics - Aurora City School District
... Each chromosome is condensed and thick, with its sister chromatids still attached at their centromeres Spindle microtubules are attached at centromeres. In each tetrad, the homologous chromosomes are held together at sites of crossing over. Within each tetrad, the spindle microtubules attach ...
... Each chromosome is condensed and thick, with its sister chromatids still attached at their centromeres Spindle microtubules are attached at centromeres. In each tetrad, the homologous chromosomes are held together at sites of crossing over. Within each tetrad, the spindle microtubules attach ...
Targeted gene expression as a means of altering cell fates and
... 1. pUAST: we constructed a vector into which genes can be subcloned behind the GAL UAS. A fragment containing five optimized GAL4 binding sites (the ‘ScaI site’ 17-mer; Webster et al., 1988) and a synthetic TATA box (Lillie and Green, 1989) separated by a unique NotI site from the SV40 terminator wa ...
... 1. pUAST: we constructed a vector into which genes can be subcloned behind the GAL UAS. A fragment containing five optimized GAL4 binding sites (the ‘ScaI site’ 17-mer; Webster et al., 1988) and a synthetic TATA box (Lillie and Green, 1989) separated by a unique NotI site from the SV40 terminator wa ...
Redalyc.Molecular Epidemiology and Evolution of Avian Infectious
... virus (IBV) is, by definition, a coronavirus of domestic fowl (Gallus gallus). Although this virus does indeed cause respiratory disease, there are strains that replicate in many non-respiratory epithelial cells, where they may cause pathologies (e.g. kidney, genital tract), while others replicate o ...
... virus (IBV) is, by definition, a coronavirus of domestic fowl (Gallus gallus). Although this virus does indeed cause respiratory disease, there are strains that replicate in many non-respiratory epithelial cells, where they may cause pathologies (e.g. kidney, genital tract), while others replicate o ...
Chapter 14
... lysogeny and the lytic cycle. • The critical event is whether cII causes sufficient synthesis of repressor to overcome the action of ...
... lysogeny and the lytic cycle. • The critical event is whether cII causes sufficient synthesis of repressor to overcome the action of ...
Exploitation of genes affecting meiotic non
... seed abortion and high frequency of additional chromosomes (reviewed in Savidan 2001). The new molecular tools developed in recent years strongly suggest that breeding efforts can be aided by direct gene transfer, and several genes of interest have been isolated and expressed in cultivated genotypes ...
... seed abortion and high frequency of additional chromosomes (reviewed in Savidan 2001). The new molecular tools developed in recent years strongly suggest that breeding efforts can be aided by direct gene transfer, and several genes of interest have been isolated and expressed in cultivated genotypes ...
during the Somatic Hypermutation Process Trends in Antibody
... scoring matrices. Mutation frequencies can be derived from a multiple sequence alignment by observing residue type variability at a given position. If this information is compiled in a residue typespecific fashion and averaged over all positions, then a global view of how easily a given residue type ...
... scoring matrices. Mutation frequencies can be derived from a multiple sequence alignment by observing residue type variability at a given position. If this information is compiled in a residue typespecific fashion and averaged over all positions, then a global view of how easily a given residue type ...
Ecological and molecular investigations of cyanotoxin production
... and are often selectively avoided [27]. As a result, zooplankton feed on phytoplankton otherwise in competition with cyanobacteria. In the process they release essential nutrients, further fertilising cyanobacterial growth. During cyanobacterial blooms, when alternative food sources for zooplankton ...
... and are often selectively avoided [27]. As a result, zooplankton feed on phytoplankton otherwise in competition with cyanobacteria. In the process they release essential nutrients, further fertilising cyanobacterial growth. During cyanobacterial blooms, when alternative food sources for zooplankton ...
Insights into Protein–DNA Interactions through Structure
... nucleotide. The interactions of the amino acid with the nucleotide can be considered at different levels: with the phosphate (p), deoxyribose sugar (S) or base (B) components individually, or with the nucleotide as a complete entity. The edges are defined upon quantification of the interaction betwe ...
... nucleotide. The interactions of the amino acid with the nucleotide can be considered at different levels: with the phosphate (p), deoxyribose sugar (S) or base (B) components individually, or with the nucleotide as a complete entity. The edges are defined upon quantification of the interaction betwe ...
Mapping the Genetic Architecture of Gene Expression in Human Liver
... well known functions, nailing down how these genes confer disease susceptibility can linger for years, or even decades, as has been the case for genes like ApoE, an Alzheimer disease susceptibility gene identified more than 15 years ago [10]. Complex networks of molecular phenotypes—gene expression ( ...
... well known functions, nailing down how these genes confer disease susceptibility can linger for years, or even decades, as has been the case for genes like ApoE, an Alzheimer disease susceptibility gene identified more than 15 years ago [10]. Complex networks of molecular phenotypes—gene expression ( ...
Major City Chiefs Position Paper on Sworn vs
... o A hybrid model, as long as all staff meet the same scientific/technical standards, can be beneficial due to the diversity of experiences. o This model also has the potential for problems, when sworn and civilian staff are performing identical functions at different pay levels. The disparity in sal ...
... o A hybrid model, as long as all staff meet the same scientific/technical standards, can be beneficial due to the diversity of experiences. o This model also has the potential for problems, when sworn and civilian staff are performing identical functions at different pay levels. The disparity in sal ...
Plant Genome Resources at the National Center for Biotechnology
... for several plant genomes. Queries may include clone names or aliases, gene names or symbols, locus identifiers, gene products or descriptions, genetic marker or GenBank identifiers. Multiple search terms can be combined using Boolean logic. Since the early 1990s, various lines of research have show ...
... for several plant genomes. Queries may include clone names or aliases, gene names or symbols, locus identifiers, gene products or descriptions, genetic marker or GenBank identifiers. Multiple search terms can be combined using Boolean logic. Since the early 1990s, various lines of research have show ...
Potential regulation of gene expression in photosynthetic cells by
... responses that should be kept in mind when planning an experimental set-up. A dark – light transition of an etiolated seedling, for instance, will activate strong photomorphogenic effects controlled by photoreceptors, which include the build-up and assembly of the photosynthetic apparatus. Therefore ...
... responses that should be kept in mind when planning an experimental set-up. A dark – light transition of an etiolated seedling, for instance, will activate strong photomorphogenic effects controlled by photoreceptors, which include the build-up and assembly of the photosynthetic apparatus. Therefore ...
Lecture9_10_extra2 - Welcome to people.pharmacy.purdue.edu!
... same gene Generation of new MHC alleles HLA B*5301Found in African populations and associated with resistance to severe malaria ...
... same gene Generation of new MHC alleles HLA B*5301Found in African populations and associated with resistance to severe malaria ...
NSC 108 - National Open University of Nigeria
... utilization of fuel molecules such as glucose. By studying and understanding these highly complex reactions, medical biochemists have found better ways to fight infections and diseases at the molecular level. Since an Engineer cannot repair a vehicle if he does not understand how it works, so a Nurs ...
... utilization of fuel molecules such as glucose. By studying and understanding these highly complex reactions, medical biochemists have found better ways to fight infections and diseases at the molecular level. Since an Engineer cannot repair a vehicle if he does not understand how it works, so a Nurs ...
Molecular Genetics of Autosomal-Dominant Demyelinating Charcot
... disorders and is the most common inherited neuromuscular disorder, with an estimated overall prevalence of 17–40/10,000. Although there has been major advances in the understanding of the genetic basis of CMT in recent years, the most useful classification is still a neurophysiological classificatio ...
... disorders and is the most common inherited neuromuscular disorder, with an estimated overall prevalence of 17–40/10,000. Although there has been major advances in the understanding of the genetic basis of CMT in recent years, the most useful classification is still a neurophysiological classificatio ...
Gene Section SHC4 (SHC (Src homology 2 domain containing) family, member 4)
... encode proteins sharing a unique structural organization. SHC proteins function as phosphotyrosine-binding docking molecule mediating the signaling transduction of cell-membrane receptors, including receptor tyrosine kinases (RTKs). SHC proteins display mutually exclusive tissue localization. SHC1 i ...
... encode proteins sharing a unique structural organization. SHC proteins function as phosphotyrosine-binding docking molecule mediating the signaling transduction of cell-membrane receptors, including receptor tyrosine kinases (RTKs). SHC proteins display mutually exclusive tissue localization. SHC1 i ...
Inferring gene-to-phenotype and gene-to
... endogenous mouse genes overlapping the deletion region. Gene-to-phenotype and gene-to-disease annotations can then be derived for the genes in nearly all genotypes with a single associated genetic locus (see docking sites below for the exception). For genotypes including more than one locus, such as ...
... endogenous mouse genes overlapping the deletion region. Gene-to-phenotype and gene-to-disease annotations can then be derived for the genes in nearly all genotypes with a single associated genetic locus (see docking sites below for the exception). For genotypes including more than one locus, such as ...
GENETIC CHARACTERIZATION OF NITRA RABBITS AND ZOBOR
... In an attempt to characterize the two rabbit breeds concerning the shift on the genetic level we studied microsatellite and genetic markers associated with coat color. These two local rabbit breeds, Nitra rabbit (Ni) and Zobor rabbit (Zo) with different phenotypes have a common origin. Based on the ...
... In an attempt to characterize the two rabbit breeds concerning the shift on the genetic level we studied microsatellite and genetic markers associated with coat color. These two local rabbit breeds, Nitra rabbit (Ni) and Zobor rabbit (Zo) with different phenotypes have a common origin. Based on the ...