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... BLAT's speed stems from an index of all nonoverlapping k-mers in the genome. This index fits inside the RAM of inexpensive computers, and need only be computed once for each genome assembly. BLAT has several major stages. It uses the index to find regions in the genome likely to be homologous to the ...
Vitis 37 (3), 119
Vitis 37 (3), 119

... S u m m a r y : Eleven decamer primers of arbitrary nucleotide sequence were used to amplify genomic DNA through the polymerase chain reaction (PCR-RAPD) in order to identify and discriminate between 14 grape cultivars (types or synonyms) belonging to the muscat family. Over 115 reproducible polymor ...
Mutational Analysis Defines the Roles of Conserved Amino Acid
Mutational Analysis Defines the Roles of Conserved Amino Acid

... domains are shown. The protein backbone is shown in the “ribbons” representation. AdoMet is shown in pink. Target adenine is shown in cyan. A, The coordinates of M.TaqI and the target adenine are taken from the crystal structure of M.TaqI complexed with DNA and a cofactor analog (PDB entry code 1g38 ...
Bio 100 Placement Study Guidelines
Bio 100 Placement Study Guidelines

... 6.! Describe the events of binary fission in prokaryotic cells. 7.! Describe how DNA folds into a visible chromosome. 8.! Explain what is happening in a eukaryotic cell at each stage of the cell cycle. 9.! Diagram and identify the phases in mitosis. 10.!Explain how cell cycle checkpoints relate to c ...
Ammonium transport in Escherichia coli: localization and nucleotide
Ammonium transport in Escherichia coli: localization and nucleotide

... (20 mM) as sole source of nitrogen, as described previously (Jayakumar et al., 1986). In complementation assays, restoration of the Amtphenotype to A52653 was monitored on M9 plates containing 100 p ~ ammonium acetate as sole nitrogen source, as previously described (Jayakumar at al., 1989). Strain ...
(S) tet Resistance Determinant Element Containing the Tetracycline
(S) tet Resistance Determinant Element Containing the Tetracycline

... initially identified in a multiresistant Listeria monocytogenes strain on a 37-kb conjugative plasmid, pIP811 (2). Subsequently tet(S) has been found on plasmid pK214 from Lactococcus lactis (8) and in the chromosome of Enterococcus faecalis (3). The tet(S) gene in L. lactis and L. monocytogenes is ...
Deletion of a conserved noncoding sequence in Plzf intron leads to
Deletion of a conserved noncoding sequence in Plzf intron leads to

... we identified 70 sequence variants (Fig. 4A and Supp. Table S1). There are 47 substitutions or SNPs in strict sense; 22 small indels (insertions and deletions up to 14 bp), from which 12 appear to be in a single base “run” and 5 in more complex ...
PhytoREF: a reference database of the plastidial 16S
PhytoREF: a reference database of the plastidial 16S

... 2011); (ii) ribosomal DNA of large protist cells (mainly heterotrophs and potentially multinucleated) or metazoans tends to be preferentially PCR-amplified because of the relatively higher copy number of ribosomal genes in these organisms (Zhu et al. 2005; Godhe et al. 2008); (iii) distinction betwe ...
Nucleosomal structure of sea urchin and starfish sperm chromatin
Nucleosomal structure of sea urchin and starfish sperm chromatin

... sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, seemed to be very similar as judged from their amino acid compositions and electrophoretic behavior in two systems. To verify the above we considered also the data available on the primar ...
Novel Blocked-Cleavable Primers for Quantitative Detection of
Novel Blocked-Cleavable Primers for Quantitative Detection of

The Mouse Immunoglobulin Heavy Chain V
The Mouse Immunoglobulin Heavy Chain V

Evolution of colour vision in primates
Evolution of colour vision in primates

... have been largely nocturnal species. For them, the ability to see in lowlight conditions was at a premium, rather than the ability to see in colour. The ancestors of modern mammals consequently lost colour vision at the time of the dinosaurs. Some primates, including humans of course, have re-evolve ...
Chapter 17 - Gene to Protein
Chapter 17 - Gene to Protein

... (c) Schematic model with mRNA and tRNA. A tRNA fits into a binding site when its anticodon base-pairs with an mRNA codon. The P site holds the tRNA attached to the growing polypeptide. The A site holds the tRNA carrying the next amino acid to be added to the polypeptide chain. Discharged tRNA leaves ...
abstracts
abstracts

... Our HortResearch fruit EST database contains over 160,000 apple sequences from 62 cDNA libraries representing several cultivars and a range of tissues. Exploitation of this resource is supported by a range of enabling technologies, including bioinformatics, biochemistry, physiology, in vivo function ...
Mendel and After - U3A Site Builder Home Page
Mendel and After - U3A Site Builder Home Page

... Linnaeus began to think the hitherto unthinkable: had God really made all these species in one creative episode or had he arranged things so that new species might arise by cross-breeding among an originally much smaller suite of primordial forms? This idea was not deemed heretical because cross-br ...
- Genetics
- Genetics

... copy was detected. One copy, denoted by E284PAR, segregates as a PAR gene, and this was used in our diversity study, using locus-specific primers. The aligned sequences of the putatively pseudoautosomal genes were first examined to see whether variants are shared between the Y and X chromosomes or are ...
Ch. 17 PPT
Ch. 17 PPT

... (c) Schematic model with mRNA and tRNA. A tRNA fits into a binding site when its anticodon base-pairs with an mRNA codon. The P site holds the tRNA attached to the growing polypeptide. The A site holds the tRNA carrying the next amino acid to be added to the polypeptide chain. Discharged tRNA leaves ...
video slide - Fayetteville State University
video slide - Fayetteville State University

... (c) Schematic model with mRNA and tRNA. A tRNA fits into a binding site when its anticodon base-pairs with an mRNA codon. The P site holds the tRNA attached to the growing polypeptide. The A site holds the tRNA carrying the next amino acid to be added to the polypeptide chain. Discharged tRNA leaves ...
Chapter 1. Fundamental Properties of Genes
Chapter 1. Fundamental Properties of Genes

... apparently neutral variation is the ability of some persons to "roll" their tongue. In general, these common alleles are roughly equivalent in function to the wild type allele. Thus they are not providing a strong selective advantage or disadvantage. All the common alleles can be considered the wild ...
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families

... An Introduction To Motif Based Functional Classification of Large Protein Families Many methods of clustering proteins within large protein families either build up from pairwise sequence alignments or rely solely on hierarchical clustering methods. While these methods can be incredible useful, they ...
Isolation and characterization of Viviparous
Isolation and characterization of Viviparous

PDF - American Society of Mammalogists
PDF - American Society of Mammalogists

... Many traits exhibit nonequivalent effects upon maternal versus paternal inheritance. Such ‘‘parent-of-origin’’ effects may be caused by several mechanisms including sex chromosomes and maternal inheritance of mitochondrial DNA. Recently, a class of mammalian autosomal genes has emerged that shows ex ...
Regulation of Gene Expression
Regulation of Gene Expression

... base pairs away from the start site. • Such long-distance transcription control elements, referred to as enhancers, are common in eukaryotic genomes but fairly rare in bacterial genomes. • An enhancer is typically 100-200 bp long. • Enhance has no functionality without promoter. • Enhances can exert ...
An improved technique for isolating codominant compound
An improved technique for isolating codominant compound

... SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for succes ...
Pattern of diversity in the genomic region near the
Pattern of diversity in the genomic region near the

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Non-coding DNA

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