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A helicoidal transfer matrix model for inhomogeneous DNA melting
A helicoidal transfer matrix model for inhomogeneous DNA melting

1_Introduction and Importance
1_Introduction and Importance

... The use of Radio Isotope labelling studies / Mass Spectroscopy / Electrophoresis / Spectrophotometry / NMR and genetic engineering methods speeded up the research on biochemistry. We are inan exciting time in Plant Biochemistry. The next century is anticipated to be "The Century of Biology" with adv ...
Week 2. DNA isolation and PCR
Week 2. DNA isolation and PCR

... calculated in their pre-laboratory assignment. Some students will not have found the reverse PCR primer because they do not understand that the reverse primer sequence needs to be reversed and complemented before they can find and exact sequence match of the primer in the gene sequence. I often have ...
is merriam`s elk really extinct?
is merriam`s elk really extinct?

... Arizona is known for producing large bulls and some people question whether a few small bands of Merriam's elk persisted until 1913 in the rugged and remote canyons of the White Mountains in eastern Arizona. If some of these remnants remained, they would have had the opportunity to interbreed with t ...
transposon
transposon

... and eukaryotes. A genome may contain both functional and nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. The fly genome has >50 types of transposon, with a total of severa ...
Retrovirus Integration Database (RID): a public database for
Retrovirus Integration Database (RID): a public database for

... the “Query option” section. For example, a nucleotide position range on a specific chromosome can be chosen to search for integration sites within a specific region of the host genome or users can search query integration sites based on genes, the PubMed ID of one or two specific publications, or a ...
Expression and purification of four different rhizobial acyl carrier
Expression and purification of four different rhizobial acyl carrier

... and the general tertiary fold of the NodF protein (Ghose et al., 1996) demonstrates that the overall structures of ACPs are surprisingly well conserved. In spite of the general structural similarity between NodF and AcpP of E. coli, AcpP cannot substitute for NodF in vivo in the synthesis of polyuns ...
Revealing the demographic histories of species
Revealing the demographic histories of species

... Review ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to th ...
Origin of New Genes: Evidence from Experimental
Origin of New Genes: Evidence from Experimental

Special Feature —Manipulating Genes to Understand
Special Feature —Manipulating Genes to Understand

... science. Transgenic animals are defined as animals in which a segment of DNA has been physically integrated into the genome of all cells, including the germ line, so that it can be transmitted to offspring as a simple Mendelian trait The DNA segment generally consists of a whole cloned gene, cDNA, o ...
and GvpD-mediated transcription regulation of the p
and GvpD-mediated transcription regulation of the p

... The transcription of the 14 p-gvp genes involved in gas vesicle formation of Halobacterium salinarum PHH1 is driven by the four promoters pA, pD, pF and pO. The regulation of these promoters was investigated in Haloferax volcanii transformants with respect to the endogenous regulatory proteins GvpE ...
Conservation and Variation in Human and Common Chimpanzee
Conservation and Variation in Human and Common Chimpanzee

... AF259061– 63, AF260135– 6, and AF350005–19 (Table II). Sequences previously reported by us have the accession numbers AF259054 – 60 and AF260134 (35) (Table II). Sequences isolated from this study and reported by others are also shown in Table II; the “NM” prefix in the accession number denotes GenB ...
Modifiers of epigenetic reprogramming show paternal effects in the mouse
Modifiers of epigenetic reprogramming show paternal effects in the mouse

... The epigenetic states of alleles that show transgenerational epigenetic inheritance, such as Avy, are sensitive to changes in dosage of chromatin proteins and enzymes involved in epigenetic reprogramming. For example, the tendency to establish a silent state can be influenced by the presence of two ...
spectroscopic studies of mosquito iridescent virus, its capsid
spectroscopic studies of mosquito iridescent virus, its capsid

... orescence excitation spectra were recorded at room temperature with a Varian Cary Eclipse fluorescent spectrophotometer, while an MPF-4 Hitachi spectrofluorimeter was used for measuring the fluorescence and phosphorescence spectra at liquid helium temperature. In both cases, a pulsed xenon lamp with ...
Site-Directed Mutagenesis Using Oligonucleotide
Site-Directed Mutagenesis Using Oligonucleotide

Can epigenetics explain transgenerational transmission of acquired
Can epigenetics explain transgenerational transmission of acquired

... More quoted than read, more misrepresented than understood, Jean-Baptiste Lamarck remains a fascinating, controversial figure in the history of evolutionary biology. For some the true founder of evolution, for others a mind prone to unrestrained flights of imagination, views and doctrines have been ...
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain

... 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been by far the most common housekeeping genetic marker used for a number of reasons. These reasons include (i) its presence in almost all bacteria, often existing as a multigene family, or operons; (ii) the function of the 16S rR ...
Application for rDNA Review/Registration for
Application for rDNA Review/Registration for

... RECOMBINANT DNA Please identify the type of experiment described in this application/registration form by checking the appropriate box(s) below. ...
Oligonucleotide Cross-Linking
Oligonucleotide Cross-Linking

Ernest Just - CPO Science
Ernest Just - CPO Science

... would be secluded from the outside world. However, monasteries were centers of learning. The monastery suited Mendel well. It was here that he conducted his famous pea experiments. Mendel’s approach to explaining his results was unique. He used math to defend what occurred naturally. This was a nove ...
Full Text  - American Diabetes Association
Full Text - American Diabetes Association

... morphogenetic protein (BMP)-signaling pathway. The approach allows us to generate testable hypotheses from GWAS candidates falling in promoter regions and has the potential to help understand the functional impact of genetic variants in DN and other complex genetic diseases. DN is the leading cause ...
Cloning in bacteria other than Escherichia coli
Cloning in bacteria other than Escherichia coli

... as a recipient for genes cloned from eukaryotes or other prokaryotes. Transformation is easy and there is available a wide range of easy-to-use vectors with specialist properties, e.g. regulatable high-level gene expression. However, use of E. coli is not always practicable because it lacks some aux ...
President`s DNA Initiative – Analyst Training
President`s DNA Initiative – Analyst Training

... capillary failure or a bad sample will be to look at the size standard peaks. • If the size standard peaks are as they should be but the samples peaks are low or appear to have lost resolution then their may be a problem with the way the sample was set up. • Also if both the size standard and sample ...
PANTHER version 11: expanded annotation data from Gene
PANTHER version 11: expanded annotation data from Gene

... tools allow users to classify new protein sequences, and to analyze gene lists obtained from large-scale genomics experiments. In the past year, major improvements include a large expansion of classification information available in PANTHER, as well as significant enhancements to the analysis tools. ...
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Non-coding DNA

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