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DNA replication
DNA replication

... Meselson-Stahl Experiment ...
03/09/05, 1 Endosymbiosis Theory I. Introduction A. Evolution of
03/09/05, 1 Endosymbiosis Theory I. Introduction A. Evolution of

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Name_______________________ Period___________ Chapter

... 1. Certain aspects of gene expression are different in eukaryotes and bacteria. To overcome differences in promoters and other DNA control sequences, scientists usually employ an expression vector, a cloning vector that contains a highly active bacterial promoter just upstream of a restriction site ...
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... fragment between the primers increases about 2-fold with each cycle. • For n = number of cycles, the amplification is approximately [2exp(n-1)]-2. • After 21 cycles, the fragment has been amplified about a million-fold. • E.g. a sample with 0.1 pg of the target fragment can be amplified to 0.1 micro ...
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... lips. Like many other viruses, HSV can remain inactive inside the body for years. When HSV becomes active, it causes cold sores around the mouth. Human cells infected with a virus may undergo programmed cell death. While HSV is inactive inside the body, only one of its genes is transcribed. This gen ...
Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD
Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD

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DNA Structure and Replication
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... parent cell. DNA replication occurs during the S phase of the cell cycle, before mitosis and cell division. The base pairing rules are crucial for the process of replication. DNA replication occurs when DNA is copied to form an identical molecule of DNA. The general steps involved in DNA replication ...
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... Characteristics of a useful plasmid. i. Single recognition site • Plasmid only cuts in one place, so this ensures that the plasmid is reformed in the correct order. ii. Origin of replication • Allows plasmid to replicate and make copies for new cells. iii. Marker genes • Identifies cells that have b ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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