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Identification of the equine herpesvirus type 1 glycoprotein 17/18 as
Identification of the equine herpesvirus type 1 glycoprotein 17/18 as

... The derived amino acid sequence of the gD homologue was almost identical to that of KyD (Audonnet et al., 1990) but differed significantly from the C terminus of KyA gD, suggesting that the latter may be atypical of EHV-1 strains. Analysis of the strain KyA using Southern blot analysis and D N A hyb ...
Syllabus, Objectives, Guide and Homework
Syllabus, Objectives, Guide and Homework

...  Distinguish between the three types of point mutations (addition, deletion, substitution) and describe how they can affect protein structure. ...
A simple set of rules for primer sequence design is as follows
A simple set of rules for primer sequence design is as follows

... The optimum length of a primer depends upon its (A+T) content, and the Tm. Apart from the Tm, a prime consideration is that the primers should be complex enough so that the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a ¼ chance of finding an A ...
myDNA
myDNA

... •make specific strings of English language letters that •are cut into patterns of shorter strips •these patterns can specifically identify individual people DNA Fingerprinting is a method where: •a person’s genetic traits, genes, are used to •make specific strings of DNA letters that •are cut into p ...
DNA SEQUENCING (using an ABI automated sequencer)
DNA SEQUENCING (using an ABI automated sequencer)

... supernatant and dry pellet. Although this method works in some cases, it is not as acceptable as column purification. Usually, some high background is present near the beginning of the sequence, so that this portion of the region is obscurred and cannot be relaibly read.] C. Dry samples. ...
The Human Genome.
The Human Genome.

... genetic codes, humans and chimpanzees are about 10 times more different than are individual humans from each other. ...
Brooker Chapter 16
Brooker Chapter 16

... In Huntington disease, the TNRE is more likely to occur if inherited from the father In myotonic muscular dystrophy, the TNRE is more likely to occur if inherited from the mother This suggests that TNRE can occur more frequently during oogenesis or spermatogenesis, depending on the gene involved. Co ...
Deletion of DNA sequences of using a polymerase chain
Deletion of DNA sequences of using a polymerase chain

... MacVector for a primer pair excluding the non-overlapping sequences permits proper binding of the primers and obtaining the desired PCR product. The advantages of this method are its high efficiency, very low cost and rapid generation of results. The efficiency is given by proof-reading polymerases ...
Kein Folientitel
Kein Folientitel

... Three clusters corresponding to one gene ...
Genetics: Inherited Traits
Genetics: Inherited Traits

TRaNsgeNIC faRm aNImal pRODUCTION aND
TRaNsgeNIC faRm aNImal pRODUCTION aND

... division) takes place based on the information as above. Obtained results also showed that double microinjection doesn’t cause significantly higher percentage of embryo degeneration. Success of the integration of a foreign gene through this method was at least 30% more than that obtained by the sing ...
Biochemistry - Problem Drill 22: DNA Question No. 1 of 10
Biochemistry - Problem Drill 22: DNA Question No. 1 of 10

... No, while this answer is correct it is not the best answer. E. Correct! Yes, all of the answers are correct, so this is the best answer choice. The characteristics of the DNA primase are listed below. It is important to memorize these and understand what each statement means. The DNA primase is a fo ...
DNA repair – providing chemical stability for life
DNA repair – providing chemical stability for life

... Using bacterial DNA which, just like human DNA, consists of nucleotides with the bases adenine, guanine, cytosine, and thymine, Tomas Lindahl began to look for repair enzymes. One chemical weakness in DNA is that cytosine easily loses an amino group, which can lead to the alteration of genetic infor ...
Leukaemia Section t(X;11)(q22;q23)  Atlas of Genetics and Cytogenetics
Leukaemia Section t(X;11)(q22;q23) Atlas of Genetics and Cytogenetics

... The chromosomal translocation t(X;11)(q22;q23) occurs very rarely, with only three cases of infants young children having been described in the literature; 2 AML cases: a 3 years old male, diagnosed with ...
Annotation Practice Activity [Based on materials from the GEP
Annotation Practice Activity [Based on materials from the GEP

... sequence of codons that results in a series of amino acid sequences in a putative polypeptide, and (3) a termination codon (UAG, UAA, UGA). The genomic sequence of a gene contains both the exons that give rise to an ORF and introns, intervening sequences between exons. During the splicing process of ...
Microarray Analysis 1
Microarray Analysis 1

... DNA microarray is a new technology to measure the level of the mRNA gene products of a living cell. A microarray chip is a rectangular chip on which is imposed a grid of DNA spots. These spots form a two dimensional array. Each spot in the array contains millions of copies of some DNA strand, bonded ...
POLYMERASE-CHAIN-REACTION (PCR) ANALYSIS OF
POLYMERASE-CHAIN-REACTION (PCR) ANALYSIS OF

... Analysis of unrelated individuals revealed femaleappearedto be homozygousin both famextensivepolymorphismat all four avian micro- ilies. satellite loci (e.g. Fig. 1). Ten Pied Flycatchers Detectionof extrapairfertilization.--Given the showed four different alleles at the PTC2 locus assumption that m ...
DNA TM Review
DNA TM Review

... Where tRNA anticodons match with mRNA codons. The mRNA is read and the code is used to build a protein. ...
Inheritance Why we look the way we do
Inheritance Why we look the way we do

... All these traits that make up how we look are carried in our DNA (deoxyribonucleic acid). ...
DNA TM Review And EXAM Review
DNA TM Review And EXAM Review

... Where tRNA anticodons match with mRNA codons. The mRNA is read and the code is used to build a protein. ...
ERF/AP2 Subfamily A3 and ER/AP2 Subfamily A6 Genes
ERF/AP2 Subfamily A3 and ER/AP2 Subfamily A6 Genes

... General seedcoat/ pre-globular stage ...
Nucleic acids and protein synthesis
Nucleic acids and protein synthesis

... The arrangement of the bases, however, does not repeat, ...
In vitro formation of a catabolic plasmid carrying
In vitro formation of a catabolic plasmid carrying

... approximately 7 kb bands (four colonies) or an 11 kb band plus a 3 kb band (eight colonies). These patterns suggested that the HindIII fragment had been incorporated in the two possible orientations. The 12 isolates were grown in Luria-Bertani medium containing ampicillin and 3-hydroxybenzoate. Male ...
Electronic supplementary material
Electronic supplementary material

... electrostatic surface potentials at helix III tend to be less positive in Ctd-TrMBF1 and hEDF1 than in the 434 repressor. This observation is consistent with the fact that the third helix contains more hydrophobic residues in Ctd-TrMBF1 and hEDF1 than in 434 repressor, especially at their N-termini ...
insertion mutation
insertion mutation

... affect on an organism? • Insertion and deletion mutations have the most effect on an organism because they affect many amino acids and consequently the whole protein. ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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