Effects of Protein-Deprivation on the Regeneration of Rat Liver after
... DNA synthesis during liver regeneration. The delay in DNA synthesis is eliminated by the administration of casein hydrolysate (given as late as 6h after partial hepatectomy), but not by glucose or incomplete amino acid mixtures. Despite the change in the timing of DNA synthesis, the increases in hep ...
... DNA synthesis during liver regeneration. The delay in DNA synthesis is eliminated by the administration of casein hydrolysate (given as late as 6h after partial hepatectomy), but not by glucose or incomplete amino acid mixtures. Despite the change in the timing of DNA synthesis, the increases in hep ...
Mechanisms of translational regulation in bacteria
... The conversion of genetic information stored in the DNA as a sequence of nucleotides into functional proteins. The synthesis of proteins requires the production of messenger RNAs (mRNAs), short-lived copies of the genes. The nucleotide sequences of these mRNAs are then converted by huge protein-RNA ...
... The conversion of genetic information stored in the DNA as a sequence of nucleotides into functional proteins. The synthesis of proteins requires the production of messenger RNAs (mRNAs), short-lived copies of the genes. The nucleotide sequences of these mRNAs are then converted by huge protein-RNA ...
Introduction to RNA sequencing
... Why sequence RNA (versus DNA)? • Interpreting mutations that do not have an obvious effect on protein sequence – ‘Regulatory’ mutations that affect what mRNA isoform is expressed and how much • e.g. splice sites, promoters, exonic/intronic splicing motifs, etc. ...
... Why sequence RNA (versus DNA)? • Interpreting mutations that do not have an obvious effect on protein sequence – ‘Regulatory’ mutations that affect what mRNA isoform is expressed and how much • e.g. splice sites, promoters, exonic/intronic splicing motifs, etc. ...
Chapter 3 Kinetic analysis of ribozyme cleavage
... It is important to recognize differences in the terms ‘catalytic RNA’ and ‘ribozyme’. Strictly speaking, a catalyst accelerates a multiple-turnover reaction without being changed itself. A few catalytic RNAs have this property, for example, RNase P and 23S rRNA; however, most ribozymes, in natural o ...
... It is important to recognize differences in the terms ‘catalytic RNA’ and ‘ribozyme’. Strictly speaking, a catalyst accelerates a multiple-turnover reaction without being changed itself. A few catalytic RNAs have this property, for example, RNase P and 23S rRNA; however, most ribozymes, in natural o ...
Modeling RNA Molecules
... who said, “The purpose of computing is insight, not numbers” (Hamming 1971), we should remember that the purpose of molecular modeling is functional insight, not detailed atomic models per se. Therefore, as we seek to improve our abilities to construct 3D models for molecules for which we do not yet ...
... who said, “The purpose of computing is insight, not numbers” (Hamming 1971), we should remember that the purpose of molecular modeling is functional insight, not detailed atomic models per se. Therefore, as we seek to improve our abilities to construct 3D models for molecules for which we do not yet ...
Module 1 - Bioinformatics.ca
... Why sequence RNA (versus DNA)? • Interpreting mutations that do not have an obvious effect on protein sequence – ‘Regulatory’ mutations that affect what mRNA isoform is expressed and how much • e.g. splice sites, promoters, exonic/intronic splicing motifs, etc. ...
... Why sequence RNA (versus DNA)? • Interpreting mutations that do not have an obvious effect on protein sequence – ‘Regulatory’ mutations that affect what mRNA isoform is expressed and how much • e.g. splice sites, promoters, exonic/intronic splicing motifs, etc. ...
1st set of Journal Clubs this Wednesday!
... Positive control: ‘An activator causes the accelerator pedal to be pushed.’ Cis-acting sequence: Can only function if on same piece of DNA that its regulating Trans-acting factor: A gene product that can act in ‘trans’ i.e. diffuse to a location at a distance from where it was made. ...
... Positive control: ‘An activator causes the accelerator pedal to be pushed.’ Cis-acting sequence: Can only function if on same piece of DNA that its regulating Trans-acting factor: A gene product that can act in ‘trans’ i.e. diffuse to a location at a distance from where it was made. ...
View Full PDF
... TFIIA, TFIIB, TFIIE, TFIIF and TFIIH [3]. RNAPII is recruited to the promoter as a holoenzyme that includes a subset of GTFs and the co-activator complex that mediates the response to transcriptional activators as well as repressors [5–7]. Eukaryotic repressors are typically modular with functionall ...
... TFIIA, TFIIB, TFIIE, TFIIF and TFIIH [3]. RNAPII is recruited to the promoter as a holoenzyme that includes a subset of GTFs and the co-activator complex that mediates the response to transcriptional activators as well as repressors [5–7]. Eukaryotic repressors are typically modular with functionall ...
Biomarker for Spinal Muscular Atrophy: Expression
... exogenous copy of SMN, or protecting motor neurons from degenerating ([11] [12] for review). Recently, small molecules that modify the splicing of SMN2 toward the inclusion of Exon 7 have been published and show clear efficacy in SMA mouse models ([13]). This approach has the potential to improve th ...
... exogenous copy of SMN, or protecting motor neurons from degenerating ([11] [12] for review). Recently, small molecules that modify the splicing of SMN2 toward the inclusion of Exon 7 have been published and show clear efficacy in SMA mouse models ([13]). This approach has the potential to improve th ...
Cloning and characterization of the Xenopus laevis p8 gene
... The p8 gene encodes a transcription factor with a basic helix-loop-helix motif and has been cloned in rat, mouse and human. It is upregulated in acute pancreatitis. In the present study, the Xenopus laevis homolog of p8 (Xp8) was isolated by PCR. The full-length Xp8 cDNA consists of 677 bp and encod ...
... The p8 gene encodes a transcription factor with a basic helix-loop-helix motif and has been cloned in rat, mouse and human. It is upregulated in acute pancreatitis. In the present study, the Xenopus laevis homolog of p8 (Xp8) was isolated by PCR. The full-length Xp8 cDNA consists of 677 bp and encod ...
Biochimica et Biophysica Acta
... This two-plasmid strategy was applied to the L30e protein and its target RNA. Although the initial two-plasmid constructs produced very low repression ratios, following optimization of the reporter construct and conditions for protein expression, a 450-fold repression of the reporter construct by L3 ...
... This two-plasmid strategy was applied to the L30e protein and its target RNA. Although the initial two-plasmid constructs produced very low repression ratios, following optimization of the reporter construct and conditions for protein expression, a 450-fold repression of the reporter construct by L3 ...
Gene Section JUND (proto-oncogene) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... (Ser90/Ser100), DNA binding domain, nuclear localization signal (NLS), and a leucine zipper domain. Since this protein lacks the JNK docking site, JUND can only be weakly phosphorylated by JNK. Although the JunD gene has no introns and produces a single transcript, the JUND mRNA translates two JUND ...
... (Ser90/Ser100), DNA binding domain, nuclear localization signal (NLS), and a leucine zipper domain. Since this protein lacks the JNK docking site, JUND can only be weakly phosphorylated by JNK. Although the JunD gene has no introns and produces a single transcript, the JUND mRNA translates two JUND ...
Characterization of the unique intron
... acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide linker (3-5). The amino acid sequence of Euglena pLHCPII was ...
... acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide linker (3-5). The amino acid sequence of Euglena pLHCPII was ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
T-box-mediated control of the anabolic proline biosynthetic genes of
... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...
... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...
Strains to Theiler`s Virus Persistent Infection the Difference of
... The D10Pas4 deletion could have been responsible for the susceptibility of the SJL/J strain for two reasons. First, it is present in the only strain for which the H-2D haplotype does not correlate with susceptibility to persistent infection. Second, it is located 44 nt upstream of the TATA box, betw ...
... The D10Pas4 deletion could have been responsible for the susceptibility of the SJL/J strain for two reasons. First, it is present in the only strain for which the H-2D haplotype does not correlate with susceptibility to persistent infection. Second, it is located 44 nt upstream of the TATA box, betw ...
LIN-28 co-transcriptionally binds primary let
... Priscilla M Van Wynsberghe, Zoya S Kai, Katlin B Massirer, Victoria H Burton, Gene W Yeo & Amy E Pasquinelli Nature structural & molecular biology, VOLUME 18, 302-308, MARCH 2011 ...
... Priscilla M Van Wynsberghe, Zoya S Kai, Katlin B Massirer, Victoria H Burton, Gene W Yeo & Amy E Pasquinelli Nature structural & molecular biology, VOLUME 18, 302-308, MARCH 2011 ...
Monocyte chemoattrac- tant protein
... both IL-12 and IFN-γ messages in the interstitium reflected the inhibition of Th1 cells and macrophage infiltration and activation. These results are consistent with those documented in MCP1-deficient MRL-lpr mice (13). Therefore, we suggest that the blockade of MCP-1 has little effect on helper T c ...
... both IL-12 and IFN-γ messages in the interstitium reflected the inhibition of Th1 cells and macrophage infiltration and activation. These results are consistent with those documented in MCP1-deficient MRL-lpr mice (13). Therefore, we suggest that the blockade of MCP-1 has little effect on helper T c ...
Turnip yellow mosaic virus RNA can be separated into two distinct
... RNA was extracted with water-saturated phenol in the presence of bentonite (0.5 mg per mg of virus) to inhibit traces of ribonucleases. The RNA in the aqueous phase was precipitated with ethanol after residual phenol had been eliminated by ether. RNA solutions were prepared in sterile distilled wate ...
... RNA was extracted with water-saturated phenol in the presence of bentonite (0.5 mg per mg of virus) to inhibit traces of ribonucleases. The RNA in the aqueous phase was precipitated with ethanol after residual phenol had been eliminated by ether. RNA solutions were prepared in sterile distilled wate ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... LHCPIIs can not be classified as type I or II based on amino acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide ...
... LHCPIIs can not be classified as type I or II based on amino acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide ...
Autoregulation of Actin Synthesis by Physiological
... decrease of G-actin (fig. 3a). On the other hand, treatment with C2 toxin increased G-actin by about 50% (fig. 3a) and decreased the F-actin content by 45% within 120 min (tab. 1). This effect was identical under normotonic as well as under hypotonic conditions (fig. 3a), indicating that C2 toxin ov ...
... decrease of G-actin (fig. 3a). On the other hand, treatment with C2 toxin increased G-actin by about 50% (fig. 3a) and decreased the F-actin content by 45% within 120 min (tab. 1). This effect was identical under normotonic as well as under hypotonic conditions (fig. 3a), indicating that C2 toxin ov ...
Microbial Genetics - MyCourses
... • Ribonucleic acid • Single-stranded nucleotide • 5-carbon ribose sugar • Contains uracil (U) instead of thymine (T) ...
... • Ribonucleic acid • Single-stranded nucleotide • 5-carbon ribose sugar • Contains uracil (U) instead of thymine (T) ...
Messenger RNA
Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression. Following transcription of primary transcript mRNA (known as pre-mRNA) by RNA polymerase, processed, mature mRNA is translated into a polymer of amino acids: a protein, as summarized in the central dogma of molecular biology.As in DNA, mRNA genetic information is in the sequence of nucleotides, which are arranged into codons consisting of three bases each. Each codon encodes for a specific amino acid, except the stop codons, which terminate protein synthesis. This process of translation of codons into amino acids requires two other types of RNA: Transfer RNA (tRNA), that mediates recognition of the codon and provides the corresponding amino acid, and ribosomal RNA (rRNA), that is the central component of the ribosome's protein-manufacturing machinery.The existence of mRNA was first suggested by Jacques Monod and François Jacob, and subsequently discovered by Jacob, Sydney Brenner and Matthew Meselson at the California Institute of Technology in 1961.